Identification of sawflies and horntails (Hymenoptera, 'Symphyta') through DNA barcodes: successes and caveats.

abstract：:The availability of numerous universal markers and suitable phylogenetic analysis methods are both very important for phylogenomics inference. Based on PCR amplification, a total of 122 markers, which were amplified in 19 representative species, were developed for Laurasiatherian phylogenomics. Subsequently, we illust...

journal_title：Molecular ecology resources

authors： Zhou X,Xu S,Zhang P,Yang G

更新日期：2011-01-01 00:00:00

abstract：:The spatial signature of microevolutionary processes structuring genetic variation may play an important role in the detection of loci under selection. However, the spatial location of samples has not yet been used to quantify this. Here, we present a new two-step method of spatial outlier detection at the individual ...

journal_title：Molecular ecology resources

authors： Wagner HH,Chávez-Pesqueira M,Forester BR

更新日期：2017-11-01 00:00:00

abstract：:Microsatellite loci were identified from the charcoal rot fungus (Macrophomina phaseolina). Primer pairs for 46 loci were developed, and of these, 13 were optimized and screened using genomic DNA from 55 fungal isolates collected predominantly from two soybean fields in Mississippi. Twelve of the optimized loci were p...

journal_title：Molecular ecology resources

authors： Baird RE,Wadl PA,Wang X,Johnson DH,Rinehart TA,Abbas HK,Shier T,Trigiano RN

更新日期：2009-05-01 00:00:00

abstract：:We developed nine new nuclear dinucleotide microsatellite loci for Castor canadensis. All loci were polymorphic, except for one. The number of alleles ranged from two to four and from five to 12 in populations from Arizona and Wisconsin, respectively. Average heterozygosity ranged from 0.13 to 0.86 per locus. Since cr...

journal_title：Molecular ecology resources

authors： Pelz-Serrano K,Munguia-Vega A,Piaggio AJ,Neubaum M,Munclinger P,Pártl A,VAN Riper Iii C,Culver M

更新日期：2009-03-01 00:00:00

abstract：:Microsatellite markers were developed to assess population structure and patterns of translocation in the introduced European common genet (Genetta genetta). Primer pairs were designed for 60 microsatellite sequences enriched for CA, GA, CATC and TAGA repeat motifs. Eleven loci that proved to be polymorphic were genot...

journal_title：Molecular ecology resources

authors： Gaubert P,Del Cerro I,Palomares F,Godoy JA

更新日期：2008-11-01 00:00:00

abstract：:This study examined the interspecific amplification of nuclear microsatellite loci developed mainly for eucalypts in the subgenus Symphyomyrtus across five species within the second most speciose subgenus, subgenus Eucalyptus. A set of eight to 10 loci, depending on taxon, have been identified that are highly variable...

journal_title：Molecular ecology resources

authors： Nevill PG,Reed A,Bossinger G,Vaillancourt RE,Larcombe M,Ades PK

更新日期：2008-11-01 00:00:00

abstract：:We implemented multilocus selection in a spatially-explicit, individual-based framework that enables multivariate environmental gradients to drive selection in many loci as a new module for the landscape genetics programs, CDPOP and CDMetaPOP. Our module simulates multilocus selection using a linear additive model, pr...

journal_title：Molecular ecology resources

authors： Landguth EL,Forester BR,Eckert AJ,Shirk AJ,Menon M,Whipple A,Day CC,Cushman SA

更新日期：2020-03-01 00:00:00

abstract：:Microsatellite markers have played a major role in ecological, evolutionary and conservation research during the past 20 years. However, technical constrains related to the use of capillary electrophoresis and a recent technological revolution that has impacted other marker types have brought to question the continued...

journal_title：Molecular ecology resources

authors： De Barba M,Miquel C,Lobréaux S,Quenette PY,Swenson JE,Taberlet P

更新日期：2017-05-01 00:00:00

abstract：:Asian arowana or dragonfish (Scleropages formosus) is an important fish species due to its unusual breeding biology and high economic value in the ornamental fish markets. In the present study, we aimed to (i) create the first transcriptome by Roche 454 pyrosequencing of Asian arowana brain and gonad samples; (ii) ide...

journal_title：Molecular ecology resources

authors： Shen XY,Kwan HY,Thevasagayam NM,Prakki SR,Kuznetsova IS,Ngoh SY,Lim Z,Feng F,Chang A,Orbán L

更新日期：2014-05-01 00:00:00

abstract：:This article documents the addition of 153 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Brassica oleracea, Brycon amazonicus, Dimorphandra wilsonii, Eupallasella percnurus, Helleborus foetidus, Ipomoea purpurea, Phrynops geoffroanus, Prochilodus...

journal_title：Molecular ecology resources

authors： Molecular Ecology Resources Primer Development Consortium.,Aksoy S,Almeida-Val VM,Azevedo VC,Baucom R,Bazaga P,Beheregaray LB,Bennetzen JL,Brassaloti RA,Burgess TI,Caccone A,Chang SM,Ciampi AY,Ciancaleoni S,Clímaco GT,Cloue

更新日期：2013-03-01 00:00:00

abstract：:Two recent articles were written in response to our paper "Breaking RAD: An evaluation of the utility of restriction site associated DNA sequencing scans of adaptation." While we agree with some of the comments made by the authors of these two response papers, we still believe caution should be employed in RADseq stud...

journal_title：Molecular ecology resources

authors： Lowry DB,Hoban S,Kelley JL,Lotterhos KE,Reed LK,Antolin MF,Storfer A

更新日期：2017-05-01 00:00:00

abstract：:We report the development of 10 polymorphic molecular markers containing short tandem repeats in the cosmopolitan reef-building coral, Pocillopora damicornis, an important model species for coral health, physiology, ecology, and genetics. The availability of polymorphic DNA markers in P. damicornis can act as impetus ...

journal_title：Molecular ecology resources

authors： Starger CJ,Yeoh SS,Dai CF,Baker AC,Desalle R

更新日期：2008-05-01 00:00:00

abstract：:This article documents the public availability of (i) microbiomes in diet and gut of larvae from the dipteran Dilophus febrilis using massive parallel sequencing, (ii) SNP and SSR discovery and characterization in the transcriptome of the Atlantic mackerel (Scomber scombrus, L) and (iii) assembled transcriptome for an...

journal_title：Molecular ecology resources

authors： Genomic Resources Development Consortium.,Álvarez P,Arthofer W,Coelho MM,Conklin D,Estonba A,Grosso AR,Helyar SJ,Langa J,Machado MP,Montes I,Pinho J,Rief A,Schartl M,Schlick-Steiner BC,Seeber J,Steiner FM,Vilas C

更新日期：2015-11-01 00:00:00

abstract：:We describe primers and polymerase chain reaction conditions to amplify 22 microsatellite loci from the Barrow's goldeneye (Bucephala islandica). The primers were tested on 27 individuals from a population breeding in British Columbia, Canada. The developed primer pairs yielded an average of 6.11 alleles per locus (ra...

journal_title：Molecular ecology resources

authors： Jaari S,Jaatinen K,Merilä J

更新日期：2009-05-01 00:00:00

abstract：:We report results of cross-species amplification in Dactylis glomerata and Lolium perenne of 12 simple sequence repeats (SSRs) isolated from Lolium multiflorum×Festuca glaucescens, 42 SSRs from Festuca arundinacea and two sequence tagged sites from Oryza sativa. We compared the transferability and diversity between D....

journal_title：Molecular ecology resources

authors： Litrico I,Bech N,Flajoulot S,Cadier D,Talon C,Gibelin C,Barre P

更新日期：2009-01-01 00:00:00

abstract：:We describe 15 polymorphic microsatellite loci from the white-ruffed manakin Corapipo altera, a common understory bird of Neotropical lowland and montane evergreen forests from eastern Honduras to northwestern Colombia. These markers were developed in order to assess population structure and genetic diversity in a fra...

journal_title：Molecular ecology resources

authors： Barnett JR,Stenzler LM,Ruiz-Gutierrez V,Bogdanowicz SM,Lovette IJ

更新日期：2008-01-01 00:00:00

abstract：:Two of the five subspecies of the western big-eared bat, Corynorhinus townsendii, are listed as federally endangered with the remaining three being of conservation concern. Knowing the degree of connectivity among populations would aid in the establishment of sound conservation and management plans for this taxon. For...

journal_title：Molecular ecology resources

authors： Piaggio AJ,Miller KE,Matocq MD,Perkins SL

更新日期：2009-01-01 00:00:00

abstract：:High-throughput sequencing technologies are a proposed solution for accessing the molecular data in historical specimens. However, degraded DNA combined with the computational demands of short-read assemblies has posed significant laboratory and bioinformatics challenges for de novo genome assembly. Linked-read or "sy...

journal_title：Molecular ecology resources

authors： Colella JP,Tigano A,MacManes MD

更新日期：2020-07-01 00:00:00

abstract：:Twelve polymorphic microsatellite loci were isolated for the bonefish, Albula vulpes using a polymerase chain reaction-based procedure. The number of alleles ranged from two to 23 (mean = 8.8) in 37 specimens from south Florida. Observed and expected heterozygosities ranged from 0.07 to 0.77 (mean = 0.42) and from 0.0...

journal_title：Molecular ecology resources

authors： Seyoum S,Wallace EM,Tringali MD

更新日期：2008-03-01 00:00:00

abstract：:The formation of chimeric sequences can create significant methodological bias in PCR-based DNA metabarcoding analyses. During mixed-template amplification of barcoding regions, chimera formation is frequent and well documented. However, profiling of fungal communities typically uses the more variable rDNA region ITS....

journal_title：Molecular ecology resources

authors： Bjørnsgaard Aas A,Davey ML,Kauserud H

更新日期：2017-07-01 00:00:00

abstract：:Restriction-site-associated DNA sequencing (RAD-seq) and related methods are revolutionizing the field of population genomics in nonmodel organisms as they allow generating an unprecedented number of single nucleotide polymorphisms (SNPs) even when no genomic information is available. Yet, RAD-seq data analyses rely o...

journal_title：Molecular ecology resources

authors： Rodríguez-Ezpeleta N,Bradbury IR,Mendibil I,Álvarez P,Cotano U,Irigoien X

更新日期：2016-07-01 00:00:00

abstract：:Karyotypic changes in chromosome number and structure are drivers in the divergent evolution of diverse plant species and lineages. This study aimed to reveal the origins of the unique karyotype (2n = 12) and phylogenetic relationships of the genus Megadenia (Brassicaceae). A high-quality chromosome-scale genome was a...

journal_title：Molecular ecology resources

authors： Yang W,Zhang L,Mandáková T,Huang L,Li T,Jiang J,Yang Y,Lysak MA,Liu J,Hu Q

更新日期：2020-11-05 00:00:00

abstract：:We introduce phylodyn, an r package for phylodynamic analysis based on gene genealogies. The package's main functionality is Bayesian nonparametric estimation of effective population size fluctuations over time. Our implementation includes several Markov chain Monte Carlo-based methods and an integrated nested Laplace...

journal_title：Molecular ecology resources

authors： Karcher MD,Palacios JA,Lan S,Minin VN

更新日期：2017-01-01 00:00:00

abstract：:We isolated and characterized 52 novel microsatellite markers from Florida largemouth bass, Micropterus salmoides floridanus, for use in conservation, management and population genetic studies. Markers were assessed in M. s. floridanus from peninsular Florida (n = 30) and averaged eight alleles per locus with observed...

journal_title：Molecular ecology resources

authors： Lutz-Carrillo DJ,Hagen C,Dueck LA,Glenn TC

更新日期：2008-01-01 00:00:00

abstract：:We combined restriction site associated DNA sequencing (RADseq) using a hypomethylation-sensitive enzyme and messenger RNA sequencing (mRNAseq) to develop molecular markers for the 16 gigabase genome of Cedrus atlantica, a conifer tree species. With each method, Illumina(®) reads from one individual were used to gener...

journal_title：Molecular ecology resources

authors： Karam MJ,Lefèvre F,Dagher-Kharrat MB,Pinosio S,Vendramin GG

更新日期：2015-05-01 00:00:00

abstract：:Data on hundreds or thousands of single nucleotide polymorphisms (SNPs) provide detailed information about the relationships between individuals, but currently few tools can turn this information into a multigenerational pedigree. I present the r package sequoia, which assigns parents, clusters half-siblings sharing a...

journal_title：Molecular ecology resources

authors： Huisman J

更新日期：2017-09-01 00:00:00

abstract：:Evolutionary alterations to cis-regulatory sequences are likely to cause adaptive phenotypic complexity, through orchestrating changes in cellular proliferation, identity and communication. For nonmodel organisms with adaptive key innovations, patterns of regulatory evolution have been predominantly limited to targete...

journal_title：Molecular ecology resources

authors： Kratochwil CF,Meyer A

更新日期：2015-07-01 00:00:00

abstract：:Species identification based on short sequences of DNA markers, that is, DNA barcoding, has emerged as an integral part of modern taxonomy. However, software for the analysis of large and multilocus barcoding data sets is scarce. The Basic Local Alignment Search Tool (BLAST) is currently the fastest tool capable of ha...

journal_title：Molecular ecology resources

authors： Fan L,Hui JH,Yu ZG,Chu KH

更新日期：2014-07-01 00:00:00

abstract：:Recent advances in high-throughput sequencing technologies have offered the possibility to generate genomewide sequence data to delineate previously unidentified genetic structure, obtain more accurate estimates of demographic parameters and to evaluate potential adaptive divergence. Here, we identified 27 556 single ...

journal_title：Molecular ecology resources

authors： Zhang BD,Xue DX,Wang J,Li YL,Liu BJ,Liu JX

更新日期：2016-05-01 00:00:00

abstract：:This article documents the public availability of approximately 15 000 polymorphic SNP loci for the bighorn sheep Ovis canadensis. ...

journal_title：Molecular ecology resources

authors： Genomic Resources Development Consortium.,Coltman DW,Hogg JT,Miller JM

更新日期：2013-09-01 00:00:00