New polymorphic microsatellite loci for the Greek smooth newt, Lissotriton vulgaris graecus, and their utility in the nominotypical subspecies.

abstract：:A multiplex haplotype-specific polymerase chain reaction (MHS-PCR) method was developed, which identified seven Clupeiform species living in the tropical Eastern Atlantic region: Sardinella aurita, Sardinella maderensis, Ethmalosa fimbriata, Sardina pilchardus, Engraulis encrasicholus, Pellonula leonensis and Ilisha a...

journal_title：Molecular ecology resources

authors： Durand JD,Diatta MA,Diop K,Trape S

更新日期：2010-05-01 00:00:00

abstract：:Linkage disequilibrium (LD, association of allelic states across loci) is poorly understood by many evolutionary biologists, but as technology for multilocus sampling improves, we ignore LD at our peril. If we sample variation at 10 loci in an organism with 20 chromosomes, we can reasonably treat them as 10 'independe...

journal_title：Molecular ecology resources

authors： Baird SJ

更新日期：2015-09-01 00:00:00

abstract：:Bactrocera cucurbitae (Coquillett) is an important insect pest of melon fruit causing extensive losses of production in Asia and Pacific areas. Twelve polymorphic microsatellite loci were isolated from an enriched DNA library of this species and their amplification characteristics are described. Genetic parameters wer...

journal_title：Molecular ecology resources

authors： Wu Y,Li Z,Wu J

更新日期：2009-09-01 00:00:00

abstract：:Microsatellite markers have played a major role in ecological, evolutionary and conservation research during the past 20 years. However, technical constrains related to the use of capillary electrophoresis and a recent technological revolution that has impacted other marker types have brought to question the continued...

journal_title：Molecular ecology resources

authors： De Barba M,Miquel C,Lobréaux S,Quenette PY,Swenson JE,Taberlet P

更新日期：2017-05-01 00:00:00

abstract：:Many plants and some animal species are polyploids. Nondisomically inherited markers (e.g. microsatellites) in such species cannot be analysed directly by standard population genetics methods developed for diploid species. One solution is to transform the polyploid codominant genotypes to pseudodiploid-dominant genoty...

journal_title：Molecular ecology resources

authors： Wang J,Scribner KT

更新日期：2014-05-01 00:00:00

abstract：:We developed 16 microsatellite markers in Cordia bifurcata, a Central and South American shrub. The markers show low polymorphism in C. bifurcata, a species suspected of self-fertilization or apomixis. Of four polymorphic loci, three had only two alleles. However, current research indicates that these markers hold val...

journal_title：Molecular ecology resources

authors： Spoon TR,Kesseli RV

更新日期：2008-09-01 00:00:00

abstract：:Genomic data sets are increasingly central to ecological and evolutionary biology, but far fewer resources are available for invertebrates. Powerful new computational tools and the rapidly decreasing cost of Illumina sequencing are beginning to change this, enabling rapid genome assembly and reference marker extractio...

journal_title：Molecular ecology resources

authors： Sun X,Ding Y,Orr MC,Zhang F

更新日期：2020-05-01 00:00:00

abstract：:Streamlining the development and genotyping of microsatellites in species for which no genetic information is available represents an important technical challenge to overcome in order to enable mainstream application of state-of-the-art population genetic analysis techniques in nonmodel organisms. Using the example o...

journal_title：Molecular ecology resources

authors： Lepais O,Bacles CF

更新日期：2011-07-01 00:00:00

abstract：:An enriched genomic library was constructed and 9 novel polymorphic tetranucleotide microsatellite markers developed for Austrocedrus chilensis, the most economically important native conifer in the Andean Patagonian region. Polymorphism was investigated for these markers in 48 individuals from two populations. Number...

journal_title：Molecular ecology resources

authors： Arana MV,Buonamici A,Sebastiani F,Alia R,Gallo LA,Marchelli P,Moreno C,Vendramin GG

更新日期：2008-07-01 00:00:00

abstract：:Pedigree reconstruction using genotypic markers has become an important tool for the study of natural populations. The nonstandard nature of the underlying statistical problems has led to the necessity of developing specialized statistical and computational methods. In this article, a new version of pedigree reconstru...

journal_title：Molecular ecology resources

authors： Almudevar A,Anderson EC

更新日期：2012-01-01 00:00:00

abstract：:A variety of challenges arise when monitoring wildlife populations for disease. Sampling tissues can be invasive to hosts, and obtaining sufficient sample sizes can be expensive and time-consuming, particularly for rare species and when pathogen prevalence is low. Environmental DNA (eDNA)-based detection of pathogens ...

journal_title：Molecular ecology resources

authors： Hall EM,Crespi EJ,Goldberg CS,Brunner JL

更新日期：2016-03-01 00:00:00

abstract：:DNA extraction from minute hymenopterans and their larvae is difficult and challenging because of their small size indicating a low amount of starting material. Hence, 11 DNA extraction methods were compared to determine their efficacy in isolating DNA. Success of each method was scored on a 2% agarose gel after PCR o...

journal_title：Molecular ecology resources

authors： Dittrich-Schröder G,Wingfield MJ,Klein H,Slippers B

更新日期：2012-01-01 00:00:00

abstract：:We report the development of 60 microsatellite markers on four species of the fungal complex Microbotryum, causing anther smut of the Caryophyllaceae. Microsatellites were found in four expressed sequence tag (EST) libraries, built from isolates of M. lychnis-dioicae, M. violaceum sensus stricto, M. lagerheimii and M....

journal_title：Molecular ecology resources

authors： Giraud T,Yockteng R,Marthey S,Chiapello H,Jonot O,Lopez-Villavicencio M,DE Vienne DM,Hood ME,Refregier G,Gendrault-Jacquemard A,Wincker P,Dossat C

更新日期：2008-03-01 00:00:00

abstract：:Restriction-site-associated DNA sequencing (RAD-seq) and related methods are revolutionizing the field of population genomics in nonmodel organisms as they allow generating an unprecedented number of single nucleotide polymorphisms (SNPs) even when no genomic information is available. Yet, RAD-seq data analyses rely o...

journal_title：Molecular ecology resources

authors： Rodríguez-Ezpeleta N,Bradbury IR,Mendibil I,Álvarez P,Cotano U,Irigoien X

更新日期：2016-07-01 00:00:00

abstract：:The sea urchin Arbacia lixula is a keystone species in Mediterranean ecosystems that drive landscape changes in littoral communities. However, genomic information available for the whole order Arbacioida is very limited. Using RNA-seq techniques, we have characterized the transcriptome of four different tissue types i...

journal_title：Molecular ecology resources

authors： Pérez-Portela R,Turon X,Riesgo A

更新日期：2016-05-01 00:00:00

abstract：:With the advent of next generation sequencing technologies, transcriptome level sequence collections are arising as prominent resources for the discovery of gene-based molecular markers. In a previous study more than 15,000 simple sequence repeats (SSRs) in expressed sequence tag (EST) sequences resulting from 454 pyr...

journal_title：Molecular ecology resources

authors： Lesser MR,Parchman TL,Buerkle CA

更新日期：2012-05-01 00:00:00

abstract：:Local adaptation is often studied via (i) multiple common garden experiments comparing performance of genotypes in different environments and (ii) sequencing genotypes from multiple locations and characterizing geographic patterns in allele frequency. Both approaches aim to characterize the same pattern (local adaptat...

journal_title：Molecular ecology resources

authors： Lasky JR,Forester BR,Reimherr M

更新日期：2018-01-01 00:00:00

abstract：:Plants stably transformed to manipulate the expression of genes mediating ecological performance have profoundly altered research in plant ecology. Agrobacterium-mediated transformation remains the most effective method of creating plants harbouring a limited number of transgene integrations of low complexity. For eco...

journal_title：Molecular ecology resources

authors： Gase K,Weinhold A,Bozorov T,Schuck S,Baldwin IT

更新日期：2011-09-01 00:00:00

abstract：:sam is a Windows program designed to detect candidate loci for selection in whole-genome scans. It also gives valuable clues as regards the ecological factors at stake in the selection process. The method used is based on multiple univariate logistic regression models to test for association between allelic frequencie...

journal_title：Molecular ecology resources

authors： Joost S,Kalbermatten M,Bonin A

更新日期：2008-09-01 00:00:00

abstract：:Microsatellite genotyping of hair and faeces using standard polymerase chain reaction (PCR) resulted in low success rates and high error rates in a 2003-2004 pilot study using noninvasive genetic sampling for the brown bear (Ursus arctos) in the Italian Alps. Thus, we evaluated the performance of multiplex pre-amplifi...

journal_title：Molecular ecology resources

authors： DE Barba M,Waits LP

更新日期：2010-07-01 00:00:00

abstract：:We developed 11 polymorphic microsatellite DNA markers for an Australian native plant, Daviesia triflora. The number of alleles per locus in 40 individuals varied from four to 19, and observed and expected heterozygosities ranged from 0.450 to 0.925 and from 0.497 to 0.899, respectively. Nine loci showed no significan...

journal_title：Molecular ecology resources

authors： He T,Krauss SL,Lamont BB

更新日期：2008-11-01 00:00:00

abstract：:The almendro (Dipteryx panamensis, Fabaceae) is a tetraploid tree native to the Atlantic lowland rainforests of Central America. We present nine microsatellite primer pairs amplified in three multiplexed reactions for 549 individuals from four sites in Costa Rica. All loci were polymorphic, ranging from three to 13 al...

journal_title：Molecular ecology resources

authors： Hanson TR,Brunsfeld SJ,Finegan B,Waits LP

更新日期：2008-03-01 00:00:00

abstract：:Oestrogenic wastewater treatment works (WwTW) effluents discharged into UK rivers have been shown to affect sexual development, including inducing intersex, in wild roach (Rutilus rutilus). This can result in a reduced breeding capability with potential population level impacts. In the absence of a sex probe for roach...

journal_title：Molecular ecology resources

authors： Lange A,Paris JR,Gharbi K,Cézard T,Miyagawa S,Iguchi T,Studholme DJ,Tyler CR

更新日期：2020-07-01 00:00:00

abstract：:Although nematodes are one of the most diverse metazoan phyla, species identification through morphology is difficult. Several genetic markers have been used for their identification, but most do not provide species-level resolution in all groups, and those that do lack primer sets effective across the phylum, preclud...

journal_title：Molecular ecology resources

authors： Prosser SW,Velarde-Aguilar MG,León-Règagnon V,Hebert PD

更新日期：2013-11-01 00:00:00

abstract：:Type specimens have high scientific importance because they provide the only certain connection between the application of a Linnean name and a physical specimen. Many other individuals may have been identified as a particular species, but their linkage to the taxon concept is inferential. Because type specimens are o...

journal_title：Molecular ecology resources

authors： Prosser SW,deWaard JR,Miller SE,Hebert PD

更新日期：2016-03-01 00:00:00

abstract：:We describe the cloning and characterization of eight novel tetranucleotide microsatellite loci in the ornate chorus frog (Pseudacris ornata). We also screened 26 loci from GenBank that were isolated from other Pseudacris species and obtained consistent product from five of these dinucleotide loci. All loci are polymo...

journal_title：Molecular ecology resources

authors： Degner JF,Hether TD,Hoffman EA

更新日期：2009-03-01 00:00:00

abstract：:Phylogenomic methods have proven useful for resolving deep nodes and recalcitrant groups in the spider tree of life. Across arachnids, transcriptomic approaches may generate thousands of loci, and target-capture methods, using the previously designed arachnid-specific probe set, can target a maximum of about 1,000 loc...

journal_title：Molecular ecology resources

authors： Kulkarni S,Wood H,Lloyd M,Hormiga G

更新日期：2020-01-01 00:00:00

abstract：:DNA barcoding has become an important current scientific trend to the understanding of the world biodiversity. In the case of mega-diverse hot spots like Mexico, this technique represents an important tool for taxonomists, allowing them to concentrate in highlighted species by the barcodes instead of analyzing entire ...

journal_title：Molecular ecology resources

authors： Elías-Gutiérrez M,León-Regagnon V

更新日期：2013-11-01 00:00:00

abstract：:Noninvasive genetic approaches continue to improve studies in molecular ecology, conservation genetics and related disciplines such as forensics and epidemiology. Noninvasive sampling allows genetic studies without disturbing or even seeing the target individuals. Although noninvasive genetic sampling has been used fo...

journal_title：Molecular ecology resources

authors： Beja-Pereira A,Oliveira R,Alves PC,Schwartz MK,Luikart G

更新日期：2009-09-01 00:00:00

abstract：:The rice stem borer, Chilo suppressalis, is one of the most damaging insect pests to rice production worldwide. Although C. suppressalis has been the focus of numerous studies examining cold tolerance and diapause, plant-insect interactions, pesticide targets and resistance, and the development of RNAi-mediated pest m...

journal_title：Molecular ecology resources

authors： Ma W,Zhao X,Yin C,Jiang F,Du X,Chen T,Zhang Q,Qiu L,Xu H,Joe Hull J,Li G,Sung WK,Li F,Lin Y

更新日期：2020-01-01 00:00:00