A newly developed genetic sex marker and its application to understanding chemically induced feminisation in roach (Rutilus rutilus).

abstract：:Mitochondrial DNA (mtDNA) has formed the backbone of phylogeographic research for many years; however, recent trends focus on genome-wide analyses. One method proposed for calibrating inferences from noisy next-generation data, such as RAD sequencing, is to compare these results with analyses of mitochondrial sequence...

journal_title：Molecular ecology resources

authors： Stobie CS,Cunningham MJ,Oosthuizen CJ,Bloomer P

更新日期：2019-01-01 00:00:00

abstract：:Streamlining the development and genotyping of microsatellites in species for which no genetic information is available represents an important technical challenge to overcome in order to enable mainstream application of state-of-the-art population genetic analysis techniques in nonmodel organisms. Using the example o...

journal_title：Molecular ecology resources

authors： Lepais O,Bacles CF

更新日期：2011-07-01 00:00:00

abstract：:Horseshoe crabs, represented by only four extant species, have existed for around 500 million years. However, their existence is now under threat because of anthropogenic activities. The availability of genomic resources for these species will be valuable in planning appropriate conservation measures. Whole-genome seq...

journal_title：Molecular ecology resources

authors： Shingate P,Ravi V,Prasad A,Tay BH,Venkatesh B

更新日期：2020-11-01 00:00:00

abstract：:We report the characterization of eight microsatellite markers in the big-headed ant Pheidole megacephala, a pest ant registered in the list of '100 of the world's worst invasive alien species'. An enrichment protocol was used to isolate microsatellite loci, and polymorphism was explored with 36 individuals collected ...

journal_title：Molecular ecology resources

authors： Fournier D,Dubois D,Aron S

更新日期：2008-07-01 00:00:00

abstract：:Asian arowana or dragonfish (Scleropages formosus) is an important fish species due to its unusual breeding biology and high economic value in the ornamental fish markets. In the present study, we aimed to (i) create the first transcriptome by Roche 454 pyrosequencing of Asian arowana brain and gonad samples; (ii) ide...

journal_title：Molecular ecology resources

authors： Shen XY,Kwan HY,Thevasagayam NM,Prakki SR,Kuznetsova IS,Ngoh SY,Lim Z,Feng F,Chang A,Orbán L

更新日期：2014-05-01 00:00:00

abstract：:Quantitative real-time PCR (qPCR) is slowly becoming established as a tool to quantify abundance of different arbuscular mycorrhizal fungal (AMF) taxa in roots and in soil. Here, we describe the development and field validation of qPCR markers (i.e. primers with associated hydrolysis probes), targeting taxon-specific ...

journal_title：Molecular ecology resources

authors： Thonar C,Erb A,Jansa J

更新日期：2012-03-01 00:00:00

abstract：:A microsatellite-enriched genomic library was developed for the water flea Daphnia atkinsoni Baird, 1859, a dominant species of intermittent wetlands in Europe. Eight polymorphic microsatellite markers were successfully optimized. Characterization of 77 individuals from Belgium and Spain showed moderate (in the former...

journal_title：Molecular ecology resources

authors： Ortells R,VAN Houdt JK,Geldof S,DE Meester L,Mergeay J

更新日期：2009-01-01 00:00:00

abstract：:Noninvasive genetic approaches continue to improve studies in molecular ecology, conservation genetics and related disciplines such as forensics and epidemiology. Noninvasive sampling allows genetic studies without disturbing or even seeing the target individuals. Although noninvasive genetic sampling has been used fo...

journal_title：Molecular ecology resources

authors： Beja-Pereira A,Oliveira R,Alves PC,Schwartz MK,Luikart G

更新日期：2009-09-01 00:00:00

abstract：:In noninvasive genetic sampling, when genotyping error rates are high and recapture rates are low, misidentification of individuals can lead to overestimation of population size. Thus, estimating genotyping errors is imperative. Nonetheless, conducting multiple polymerase chain reactions (PCRs) at multiple loci is tim...

journal_title：Molecular ecology resources

authors： Hansen H,Ben-David M,McDonald DB

更新日期：2008-03-01 00:00:00

abstract：:In this study, we reported the isolation and analysis of new polymorphic microsatellites in mungbean (Vigna radiata (L.) Wilczek). Twelve out of 210 primer pairs screened in 30 mungbean accessions gave polymorphism. The polymorphic markers detected two to three alleles per locus with an average of 2.08. Observed heter...

journal_title：Molecular ecology resources

authors： Somta P,Musch W,Kongsamai B,Chanprame S,Nakasathien S,Toojinda T,Sorajjapinun W,Seehalak W,Tragoonrung S,Srinives P

更新日期：2008-09-01 00:00:00

abstract：:Betel nut (Areca nut, Areca catechu L.) is a conspicuous and important cultivated plant of tropical and subtropical habitats throughout Southeast Asia and Oceania. As a significant cultural and social offering, the migration of betel nut associated with human dispersal is an important issue in ethnobotany and anthropo...

journal_title：Molecular ecology resources

authors： Hu CH,Huang CC,Hung KH,Hsu TW,Chiang TY

更新日期：2009-03-01 00:00:00

abstract：:Sex-linked molecular markers have become valuable tools for understanding sex ratio evolution and sex-specific physiology in pre-reproductive plants. To develop new accurate methods for sexing Distichlis spicata juveniles and nonflowering individuals, we converted a random amplified polymorphic DNA-polymerase chain re...

journal_title：Molecular ecology resources

authors： Eppley SM,O'Quinn R,Brown AL

更新日期：2009-09-01 00:00:00

abstract：:Palaeoenvironments and former climates are typically inferred from pollen and macrofossil records. This approach is time-consuming and suffers from low taxonomic resolution and biased taxon sampling. Here, we test an alternative DNA-based approach utilizing the P6 loop in the chloroplast trnL (UAA) intron; a short (13...

journal_title：Molecular ecology resources

authors： Sønstebø JH,Gielly L,Brysting AK,Elven R,Edwards M,Haile J,Willerslev E,Coissac E,Rioux D,Sannier J,Taberlet P,Brochmann C

更新日期：2010-11-01 00:00:00

abstract：:Inventory of the caterpillars, their food plants and parasitoids began in 1978 for today's Area de Conservacion Guanacaste (ACG), in northwestern Costa Rica. This complex mosaic of 120 000 ha of conserved and regenerating dry, cloud and rain forest over 0-2000 m elevation contains at least 10 000 species of non-leaf-m...

journal_title：Molecular ecology resources

authors： Janzen DH,Hallwachs W,Blandin P,Burns JM,Cadiou JM,Chacon I,Dapkey T,Deans AR,Epstein ME,Espinoza B,Franclemont JG,Haber WA,Hajibabaei M,Hall JP,Hebert PD,Gauld ID,Harvey DJ,Hausmann A,Kitching IJ,Lafontaine D,Lan

更新日期：2009-05-01 00:00:00

abstract：:The brushtail possum is a major agricultural and ecological pest in New Zealand. A novel noninvasive DNA sampling tool for detecting its presence (WaxTags, or WT) was tested. DNA was recovered from saliva left on WT, and two lengths (407 bp and 648 bp) of the cytochrome c oxidase I (COI) barcoding region were amplifie...

journal_title：Molecular ecology resources

authors： Vargas ML,Cruickshank RH,Ross JG,Holyoake AJ,Ogilvie SC,Paterson AM

更新日期：2009-03-01 00:00:00

abstract：:Simple sequence repeats (SSRs) derived from expressed sequence tags (ESTs) are valuable markers because they represent transcribed regions and often transferable to related taxa. Here, we report the development and characterization of EST-SSRs from Shorea leprosula. Fifty-four sequences containing SSRs were identified...

journal_title：Molecular ecology resources

authors： Ng KK,Lee SL,Tsumura Y,Ueno S,Ng CH,Lee CT

更新日期：2009-01-01 00:00:00

abstract：:Anolis lizards are important models in studies of ecology and evolution. Here we describe 13 polymorphic microsatellites for use in population screening in the St Lucia anole, Anolis luciae, that can be used as a natural replicate to Anolis roquet on Martinique to study processes involved in population differentiation...

journal_title：Molecular ecology resources

authors： Johansson H,Surget-Groba Y,Gow JL,Thorpe RS

更新日期：2008-11-01 00:00:00

abstract：:We developed 11 polymorphic microsatellite DNA markers for an Australian native plant, Daviesia triflora. The number of alleles per locus in 40 individuals varied from four to 19, and observed and expected heterozygosities ranged from 0.450 to 0.925 and from 0.497 to 0.899, respectively. Nine loci showed no significan...

journal_title：Molecular ecology resources

authors： He T,Krauss SL,Lamont BB

更新日期：2008-11-01 00:00:00

abstract：:Fifteen polymorphic markers were developed from a microsatellite-enriched library for the lizard Podarcis gaigeae. The loci were checked for variability in 68 individuals from a population on the island of Skyros, Greece. The number of alleles ranged from 3 to 23 per locus and expected heterozygosity from 0.29 and 0.9...

journal_title：Molecular ecology resources

authors： Wellenreuther M,Runemark A,Svensson EI,Hansson B

更新日期：2009-05-01 00:00:00

abstract：:High-throughput sequencing has changed many aspects of population genetics, molecular ecology and related fields, affecting both experimental design and data analysis. The software package angsd allows users to perform a number of population genetic analyses on high-throughput sequencing data. angsd uses probabilistic...

journal_title：Molecular ecology resources

authors： Durvasula A,Hoffman PJ,Kent TV,Liu C,Kono TJ,Morrell PL,Ross-Ibarra J

更新日期：2016-11-01 00:00:00

abstract：:Mites (Acari) are one of the most diverse groups of life on Earth; yet, their evolutionary relationships are poorly understood. Also, the resolution of broader arachnid phylogeny has been hindered by an underrepresentation of mite diversity in phylogenomic analyses. To further our understanding of Acari evolution, we ...

journal_title：Molecular ecology resources

authors： Van Dam MH,Trautwein M,Spicer GS,Esposito L

更新日期：2019-03-01 00:00:00

abstract：:Restriction-site-associated DNA sequencing (RAD-seq) and related methods are revolutionizing the field of population genomics in nonmodel organisms as they allow generating an unprecedented number of single nucleotide polymorphisms (SNPs) even when no genomic information is available. Yet, RAD-seq data analyses rely o...

journal_title：Molecular ecology resources

authors： Rodríguez-Ezpeleta N,Bradbury IR,Mendibil I,Álvarez P,Cotano U,Irigoien X

更新日期：2016-07-01 00:00:00

abstract：:Karyotypic changes in chromosome number and structure are drivers in the divergent evolution of diverse plant species and lineages. This study aimed to reveal the origins of the unique karyotype (2n = 12) and phylogenetic relationships of the genus Megadenia (Brassicaceae). A high-quality chromosome-scale genome was a...

journal_title：Molecular ecology resources

authors： Yang W,Zhang L,Mandáková T,Huang L,Li T,Jiang J,Yang Y,Lysak MA,Liu J,Hu Q

更新日期：2020-11-05 00:00:00

abstract：:Biodiversity analyses based on next-generation sequencing (NGS) platforms have developed by leaps and bounds in recent years. A PCR-free strategy, which can alleviate taxonomic bias, was considered as a promising approach to delivering reliable species compositions of targeted environments. The major impediment of suc...

journal_title：Molecular ecology resources

authors： Liu S,Wang X,Xie L,Tan M,Li Z,Su X,Zhang H,Misof B,Kjer KM,Tang M,Niehuis O,Jiang H,Zhou X

更新日期：2016-03-01 00:00:00

abstract：:The development of genetic markers has revolutionized molecular studies within and among populations. Although poly-allelic microsatellites are the most commonly used genetic marker for within-population studies of free-living animals, biallelic single nucleotide polymorphisms, or SNPs, have also emerged as a viable o...

journal_title：Molecular ecology resources

authors： Weinman LR,Solomon JW,Rubenstein DR

更新日期：2015-05-01 00:00:00

abstract：:The protist Labyrinthula zosterae (Phylum Bigyra, sensu Tsui et al. 2009) has been identified as a causative agent of wasting disease in eelgrass (Zostera marina), of which the most intense outbreak led to the destruction of 90% of eelgrass beds in eastern North America and western Europe in the 1930s. Outbreaks still...

journal_title：Molecular ecology resources

authors： Bergmann N,Fricke B,Schmidt MC,Tams V,Beining K,Schwitte H,Boettcher AA,Martin DL,Bockelmann AC,Reusch TB,Rauch G

更新日期：2011-11-01 00:00:00

abstract：:Genomic data sets are increasingly central to ecological and evolutionary biology, but far fewer resources are available for invertebrates. Powerful new computational tools and the rapidly decreasing cost of Illumina sequencing are beginning to change this, enabling rapid genome assembly and reference marker extractio...

journal_title：Molecular ecology resources

authors： Sun X,Ding Y,Orr MC,Zhang F

更新日期：2020-05-01 00:00:00

abstract：:The spatial signature of microevolutionary processes structuring genetic variation may play an important role in the detection of loci under selection. However, the spatial location of samples has not yet been used to quantify this. Here, we present a new two-step method of spatial outlier detection at the individual ...

journal_title：Molecular ecology resources

authors： Wagner HH,Chávez-Pesqueira M,Forester BR

更新日期：2017-11-01 00:00:00

abstract：:The concept of effective population size (N(e) ) is based on an elegantly simple idea which, however, rapidly becomes very complex when applied to most real-world situations. In natural populations, spatial and temporal stratifications create different classes of individuals with different vital rates, and this in tur...

journal_title：Molecular ecology resources

authors： Waples RS

更新日期：2010-09-01 00:00:00

abstract：:Long a major focus of genetic research and breeding, sunflowers (Helianthus) are emerging as an increasingly important experimental system for ecological and evolutionary studies. Here, we review the various attributes of wild and domesticated sunflowers that make them valuable for ecological experimentation and descr...

journal_title：Molecular ecology resources

authors： Kane NC,Burke JM,Marek L,Seiler G,Vear F,Baute G,Knapp SJ,Vincourt P,Rieseberg LH

更新日期：2013-01-01 00:00:00