Abstract:
:In this study, we reported the isolation and analysis of new polymorphic microsatellites in mungbean (Vigna radiata (L.) Wilczek). Twelve out of 210 primer pairs screened in 30 mungbean accessions gave polymorphism. The polymorphic markers detected two to three alleles per locus with an average of 2.08. Observed heterozygosity varied from 0 to 0.133, while expected heterozygosity ranged from 0.095 to 0.498. Tests for Hardy-Weinberg equilibrium (HWE) and pairwise linkage disequilibrium of the polymorphic loci revealed that all loci except MB-SSR14 significantly departed from HWE and four pairwise combinations, viz. MB-SSR14 vs. MB-SSR42, MB-SSR42 vs. MB-SSR87, MB-SSR114 vs. MB-SSR121, and MB-SSR175 vs. MB-SSR231 significantly deviated from linkage disequilibrium. The markers are being used to study genetic diversity and genome mapping of mungbean.
journal_name
Mol Ecol Resourjournal_title
Molecular ecology resourcesauthors
Somta P,Musch W,Kongsamai B,Chanprame S,Nakasathien S,Toojinda T,Sorajjapinun W,Seehalak W,Tragoonrung S,Srinives Pdoi
10.1111/j.1755-0998.2008.02219.xsubject
Has Abstractpub_date
2008-09-01 00:00:00pages
1155-7issue
5eissn
1755-098Xissn
1755-0998journal_volume
8pub_type
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