Abstract:
:In this study, 49 primers were designed from sequences containing di-, tri-, tetra-, penta- and hexanucleotide motifs with a minimum of four repeats and presence of motif size polymorphisms (insertion/deletion) from cassava (Manihot esculenta Crantz) expressed sequence tags deposited in public sequence database. Each locus was subsequently screened on 29 M. esculenta Crantz obtained from 15 different countries. Cross-amplification was tested with M. esculenta Crantz (ssp. flabellifolia) and four different Manihot species, M. chlorosticta, M. carthaginensis, M. filamentosa and M. tristis. Of these, nine loci showed polymorphic profiles within M. esculenta Crantz, which revealed two to four alleles per locus. The average unbiased and direct count heterozygosities were 0.4901 and 0.5674, respectively.
journal_name
Mol Ecol Resourjournal_title
Molecular ecology resourcesauthors
Tangphatsornruang S,Sraphet S,Singh R,Okogbenin E,Fregene M,Triwitayakorn Kdoi
10.1111/j.1471-8286.2007.02047.xsubject
Has Abstractpub_date
2008-05-01 00:00:00pages
682-5issue
3eissn
1755-098Xissn
1755-0998journal_volume
8pub_type
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