Abstract:
:This study reports the isolation and characterization of 11 polymorphic microsatellites from a sand smelt (Atherina boyeri) genomic library. Enrichment was performed with di-, tri- and tetranucleotide motifs following the FIASCO procedure (fast isolation by AFLP of sequences containing repeats). All loci were found to be in linkage and in Hardy-Weinberg equilibrium. This represents the first microsatellite isolation for the family Atherinidae and the isolated loci were accordingly tested on four additional species of the family: two recognized (A. presbyter and A. hepsetus) and two proposed ('punctata' and 'non-punctata' forms). Moreover their cross-species suitability on Menidia menidia, belonging to the same order but to the family Atherinopsidae, was also tested.
journal_name
Mol Ecol Resourjournal_title
Molecular ecology resourcesauthors
Milana V,Sola L,Rossi AR,Barbisan F,Congiu Ldoi
10.1111/j.1755-0998.2008.02366.xsubject
Has Abstractpub_date
2009-05-01 00:00:00pages
889-92issue
3eissn
1755-098Xissn
1755-0998journal_volume
9pub_type
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