High-throughput identification and diagnostics of pathogens and pests: Overview and practical recommendations.

abstract：:The brushtail possum is a major agricultural and ecological pest in New Zealand. A novel noninvasive DNA sampling tool for detecting its presence (WaxTags, or WT) was tested. DNA was recovered from saliva left on WT, and two lengths (407 bp and 648 bp) of the cytochrome c oxidase I (COI) barcoding region were amplifie...

journal_title：Molecular ecology resources

authors： Vargas ML,Cruickshank RH,Ross JG,Holyoake AJ,Ogilvie SC,Paterson AM

更新日期：2009-03-01 00:00:00

abstract：:Local adaptation is often studied via (i) multiple common garden experiments comparing performance of genotypes in different environments and (ii) sequencing genotypes from multiple locations and characterizing geographic patterns in allele frequency. Both approaches aim to characterize the same pattern (local adaptat...

journal_title：Molecular ecology resources

authors： Lasky JR,Forester BR,Reimherr M

更新日期：2018-01-01 00:00:00

abstract：:DNA sourced from faeces is notoriously less reliable than that from tissue. Hence, understanding whether faecal pellet quality varies within faecal piles may be important for sample selection. We hypothesized that the order in which faecal pellets are deposited may influence microsatellite polymerase chain reaction (P...

journal_title：Molecular ecology resources

authors： Walker FM,Horsup A,Taylor AC

更新日期：2009-05-01 00:00:00

abstract：:DNA barcoding is a global initiative that provides a standardized and efficient tool to catalogue and inventory biodiversity, with significant conservation applications. Despite progress across taxonomic realms, globally threatened marine turtles remain underrepresented in this effort. To obtain DNA barcodes of marine...

journal_title：Molecular ecology resources

authors： Naro-Maciel E,Reid B,Fitzsimmons NN,LE M,Desalle R,Amato G

更新日期：2010-03-01 00:00:00

abstract：:We report the characterization of 25 microsatellite loci isolated from the macaroni penguin (Eudyptes chrysolophus). Thirteen loci were arranged into four multiplex sets for future genetic studies of macaroni penguin populations. All 25 loci were tested separately in each of four other penguin species [Adélie penguin ...

journal_title：Molecular ecology resources

authors： Ahmed S,Hart T,Dawson DA,Horsburgh GJ,Trathan PN,Rogers AD

更新日期：2009-11-01 00:00:00

abstract：:We developed and tested 14 new polymorphic microsatellite loci for dreissenid mussels, including the two species that have invaded many freshwater habitats in Eurasia and North America, where they cause serious industrial fouling damage and ecological alterations. These new loci will aid our understanding of their gen...

journal_title：Molecular ecology resources

authors： Feldheim KA,Brown JE,Murphy DJ,Stepien CA

更新日期：2011-07-01 00:00:00

abstract：:Knowledge of population demographics is important for species management but can be challenging in low-density, wide-ranging species. Population monitoring of the endangered Sonoran pronghorn (Antilocapra americana sonoriensis) is critical for assessing the success of recovery efforts, and noninvasive DNA sampling (ND...

journal_title：Molecular ecology resources

authors： Woodruff SP,Johnson TR,Waits LP

更新日期：2015-07-01 00:00:00

abstract：:Horseshoe crabs, represented by only four extant species, have existed for around 500 million years. However, their existence is now under threat because of anthropogenic activities. The availability of genomic resources for these species will be valuable in planning appropriate conservation measures. Whole-genome seq...

journal_title：Molecular ecology resources

authors： Shingate P,Ravi V,Prasad A,Tay BH,Venkatesh B

更新日期：2020-11-01 00:00:00

abstract：:We developed a total of 50 microsatellite markers for the three fungal pathogens causing the most important leaf spot diseases of banana: 32 loci for Mycosphaerella fijiensis are presented, and nine loci each for Mycosphaerella musicola and Mycosphaerella eumusae. All these loci were polymorphic within each species on...

journal_title：Molecular ecology resources

authors： Zapater MF,Duchemin M,Dussart JF,Coste D,Brottier P,Carlier J

更新日期：2008-09-01 00:00:00

abstract：:Estimation of demographic history from nucleotide sequences represents an important component of many studies in molecular ecology. For example, knowledge of a population's history can allow us to test hypotheses about the impact of climatic and anthropogenic factors. In the past, demographic analysis was typically li...

journal_title：Molecular ecology resources

authors： Ho SY,Shapiro B

更新日期：2011-05-01 00:00:00

abstract：:Palaeoenvironments and former climates are typically inferred from pollen and macrofossil records. This approach is time-consuming and suffers from low taxonomic resolution and biased taxon sampling. Here, we test an alternative DNA-based approach utilizing the P6 loop in the chloroplast trnL (UAA) intron; a short (13...

journal_title：Molecular ecology resources

authors： Sønstebø JH,Gielly L,Brysting AK,Elven R,Edwards M,Haile J,Willerslev E,Coissac E,Rioux D,Sannier J,Taberlet P,Brochmann C

更新日期：2010-11-01 00:00:00

abstract：:The emergence of third-generation sequencing (3GS; long-reads) is bringing closer the goal of chromosome-size fragments in de novo genome assemblies. This allows the exploration of new and broader questions on genome evolution for a number of nonmodel organisms. However, long-read technologies result in higher sequenc...

journal_title：Molecular ecology resources

authors： Jaworski CC,Allan CW,Matzkin LM

更新日期：2020-09-01 00:00:00

abstract：:Global climate change (GCC) may be causing distribution range shifts in many organisms worldwide. Multiple efforts are currently focused on the development of models to better predict distribution range shifts due to GCC. We addressed this issue by including intraspecific genetic structure and spatial autocorrelation ...

journal_title：Molecular ecology resources

authors： Martínez-Minaya J,Conesa D,Fortin MJ,Alonso-Blanco C,Picó FX,Marcer A

更新日期：2019-07-01 00:00:00

abstract：:QST , a measure of quantitative genetic differentiation among populations, is an index that can suggest local adaptation if QST for a trait is sufficiently larger than the mean FST of neutral genetic markers. A previous method by Whitlock and Guillaume derived a simulation resampling approach to statistically test for...

journal_title：Molecular ecology resources

authors： Gilbert KJ,Whitlock MC

更新日期：2015-03-01 00:00:00

abstract：:We isolated and characterized 52 novel microsatellite markers from Florida largemouth bass, Micropterus salmoides floridanus, for use in conservation, management and population genetic studies. Markers were assessed in M. s. floridanus from peninsular Florida (n = 30) and averaged eight alleles per locus with observed...

journal_title：Molecular ecology resources

authors： Lutz-Carrillo DJ,Hagen C,Dueck LA,Glenn TC

更新日期：2008-01-01 00:00:00

abstract：:GONe is a user-friendly, Windows-based program for estimating effective size (N(e) ) in populations with overlapping generations. It uses the Jorde-Ryman modification to the temporal method to account for age structure in populations. This method requires estimates of age-specific survival and birth rate and allele fr...

journal_title：Molecular ecology resources

authors： Coombs JA,Letcher BH,Nislow KH

更新日期：2012-01-01 00:00:00

abstract：:Sex-linked molecular markers have become valuable tools for understanding sex ratio evolution and sex-specific physiology in pre-reproductive plants. To develop new accurate methods for sexing Distichlis spicata juveniles and nonflowering individuals, we converted a random amplified polymorphic DNA-polymerase chain re...

journal_title：Molecular ecology resources

authors： Eppley SM,O'Quinn R,Brown AL

更新日期：2009-09-01 00:00:00

abstract：:Nematodes play an important role in ecosystem processes, yet the relevance of nematode species diversity to ecology is unknown. Because nematode identification of all individuals at the species level using standard techniques is difficult and time-consuming, nematode communities are not resolved down to the species le...

journal_title：Molecular ecology resources

authors： Porazinska DL,Giblin-Davis RM,Faller L,Farmerie W,Kanzaki N,Morris K,Powers TO,Tucker AE,Sung W,Thomas WK

更新日期：2009-11-01 00:00:00

abstract：:Publilia concava is an eastern North American membracid commonly occurring in large but spatially patchy aggregations, primarily on the host plant Solidago altissima. Like other myrmecophiles, P. concava provides sugary excretions to ants in return for the various protective, competitive or even sanitary benefits that...

journal_title：Molecular ecology resources

authors： Chhatre V,Morales MA,Abbot P

更新日期：2009-07-01 00:00:00

abstract：:Long columns of migrating larval sciarid armyworms were discovered in central and northern Japan, specifically Kanagawa, Gunma, Miyagi and Akita prefectures, as well as Hokkaido. This is the first examination of armyworms in East Asia. In Europe, armyworms have been identified as Sciara militaris, belonging to the fam...

journal_title：Molecular ecology resources

authors： Sutou M,Kato T,Ito M

更新日期：2011-11-01 00:00:00

abstract：:Many plants and some animal species are polyploids. Nondisomically inherited markers (e.g. microsatellites) in such species cannot be analysed directly by standard population genetics methods developed for diploid species. One solution is to transform the polyploid codominant genotypes to pseudodiploid-dominant genoty...

journal_title：Molecular ecology resources

authors： Wang J,Scribner KT

更新日期：2014-05-01 00:00:00

abstract：:Asian arowana or dragonfish (Scleropages formosus) is an important fish species due to its unusual breeding biology and high economic value in the ornamental fish markets. In the present study, we aimed to (i) create the first transcriptome by Roche 454 pyrosequencing of Asian arowana brain and gonad samples; (ii) ide...

journal_title：Molecular ecology resources

authors： Shen XY,Kwan HY,Thevasagayam NM,Prakki SR,Kuznetsova IS,Ngoh SY,Lim Z,Feng F,Chang A,Orbán L

更新日期：2014-05-01 00:00:00

abstract：:The 'One Health' framework emphasizes the ecological relationships between soil, plant, animal and human health. Microbiomes play important roles in these relationships, as they modify the health and performance of the different compartments and influence the transfer of energy, matter and chemicals between them. Stan...

journal_title：Molecular ecology resources

authors： Wasimuddin,Schlaeppi K,Ronchi F,Leib SL,Erb M,Ramette A

更新日期：2020-11-01 00:00:00

abstract：:Noninvasive genetic sampling has increasingly been used in ecological and conservation studies during the last decade. A major part of the noninvasive genetic literature is dedicated to the search for optimal protocols, by comparing different methods of collection, preservation and extraction of DNA from noninvasive m...

journal_title：Molecular ecology resources

authors： Renan S,Speyer E,Shahar N,Gueta T,Templeton AR,Bar-David S

更新日期：2012-11-01 00:00:00

abstract：:We describe the cloning and characterization of eight novel tetranucleotide microsatellite loci in the ornate chorus frog (Pseudacris ornata). We also screened 26 loci from GenBank that were isolated from other Pseudacris species and obtained consistent product from five of these dinucleotide loci. All loci are polymo...

journal_title：Molecular ecology resources

authors： Degner JF,Hether TD,Hoffman EA

更新日期：2009-03-01 00:00:00

abstract：:Eleven microsatellites were characterized for Semicossyphus pulcher (California sheephead) using an enrichment protocol. The number of alleles varied from three to 14 for a sample of 40 individuals from two populations. Expected heterozygosities ranged from 0.311 to 0.891. All loci but one were in Hardy-Weinberg equil...

journal_title：Molecular ecology resources

authors： Poortvliet M,Olsen JL,Selkoe KA,Coyer JA,Bernardi G

更新日期：2009-01-01 00:00:00

abstract：:Female Drosophila melanogaster frequently mate with multiple males in nature as shown through parentage analysis. Although polyandry is well documented, we know little about the timing between mating events in wild Drosophila populations due to the challenge of following behaviours of individual females. In this study...

journal_title：Molecular ecology resources

authors： Giardina TJ,Clark AG,Fiumera AC

更新日期：2017-11-01 00:00:00

abstract：:Crepidula convexa, a calyptreid gastropod with direct embryonic development, changes sex from male to female in the course of its lifetime (protandry). Under sex-allocation theory, male reproductive success should be independent from age and size (a proxy used for age). However, this may be counterbalanced by female c...

journal_title：Molecular ecology resources

authors： Daguin-Thiébaut C,LE Cam S,Viard F

更新日期：2009-05-01 00:00:00

abstract：:This article documents the whole genome sequence information of the Indian Zaprionus indianus, a member of the fruit fly family Drosophilidae. The sequences were generated on an Illumina platform and reads and whole genome sequence submitted to NCBI to the SRA and BioProject databases, respectively. This is the first ...

journal_title：Molecular ecology resources

authors： Khanna R,Mohanty S

更新日期：2017-05-01 00:00:00

abstract：:Camelids are characterized by their unique adaptive immune system that exhibits the generation of homodimeric heavy-chain immunoglobulins, somatic hypermutation of T-cell receptors, and low genetic diversity of major histocompatibility complex (MHC) genes. However, short-read assemblies are typically highly fragmented...

journal_title：Molecular ecology resources

authors： Ming L,Wang Z,Yi L,Batmunkh M,Liu T,Siren D,He J,Juramt N,Jambl T,Li Y,Jirimutu

更新日期：2020-05-01 00:00:00