Recent discoveries of armyworms in Japan and their species identification using DNA barcoding.

Abstract:

:Long columns of migrating larval sciarid armyworms were discovered in central and northern Japan, specifically Kanagawa, Gunma, Miyagi and Akita prefectures, as well as Hokkaido. This is the first examination of armyworms in East Asia. In Europe, armyworms have been identified as Sciara militaris, belonging to the family Sciaridae (sciarid flies or black fungus gnats), by rearing them to adulthood. In Japan, we were unable to obtain live samples for rearing; therefore, DNA barcodes were obtained from the samples of armyworms collected in the Gunma and Miyagi prefectures. The DNA barcodes were compared with those obtained from the following samples: pupae of S. militaris from UK, adults of Sciara kitakamiensis, Sciara humeralis, Sciara hemerobioides, Sciara thoracica, Sciara helvola and Sciara melanostyla from Japan, and adults of one undescribed Sciara species from Malaysia. Neighbour-joining, maximum parsimony, and maximum likelihood analyses revealed that the armyworms discovered in Japan are S. kitakamiensis. Although adults of this species have been recorded in several locations in Japan, this is the first report of migrating larval armyworms. DNA barcodes were effectively used to link different life stages of this species. The average intraspecific and interspecific pairwise genetic distances of the genus Sciara were 0.3% and 12.6%, respectively. The present study illustrates that DNA barcodes are an effective means of identifying sciarid flies in Japan.

journal_name

Mol Ecol Resour

authors

Sutou M,Kato T,Ito M

doi

10.1111/j.1755-0998.2011.03040.x

subject

Has Abstract

pub_date

2011-11-01 00:00:00

pages

992-1001

issue

6

eissn

1755-098X

issn

1755-0998

journal_volume

11

pub_type

杂志文章
  • Developing EPIC markers for chalcidoid Hymenoptera from EST and genomic data.

    abstract::Increasing numbers of phylogeographic studies make comparative inferences about the histories of co-distributed species. Although the aims of such studies are best achieved by jointly analysing sequences from multiple loci in a model-based framework, such data currently exist for few nonmodel systems. We used existing...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2010.02956.x

    authors: Lohse K,Sharanowski B,Blaxter M,Nicholls JA,Stone GN

    更新日期:2011-05-01 00:00:00

  • Microsatellites from the charcoal rot fungus (Macrophomina phaseolina).

    abstract::Microsatellite loci were identified from the charcoal rot fungus (Macrophomina phaseolina). Primer pairs for 46 loci were developed, and of these, 13 were optimized and screened using genomic DNA from 55 fungal isolates collected predominantly from two soybean fields in Mississippi. Twelve of the optimized loci were p...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2008.02511.x

    authors: Baird RE,Wadl PA,Wang X,Johnson DH,Rinehart TA,Abbas HK,Shier T,Trigiano RN

    更新日期:2009-05-01 00:00:00

  • A comparison of single nucleotide polymorphism and microsatellite markers for analysis of parentage and kinship in a cooperatively breeding bird.

    abstract::The development of genetic markers has revolutionized molecular studies within and among populations. Although poly-allelic microsatellites are the most commonly used genetic marker for within-population studies of free-living animals, biallelic single nucleotide polymorphisms, or SNPs, have also emerged as a viable o...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12330

    authors: Weinman LR,Solomon JW,Rubenstein DR

    更新日期:2015-05-01 00:00:00

  • Finding stories in noise: Mitochondrial portraits from RAD data.

    abstract::Mitochondrial DNA (mtDNA) has formed the backbone of phylogeographic research for many years; however, recent trends focus on genome-wide analyses. One method proposed for calibrating inferences from noisy next-generation data, such as RAD sequencing, is to compare these results with analyses of mitochondrial sequence...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12953

    authors: Stobie CS,Cunningham MJ,Oosthuizen CJ,Bloomer P

    更新日期:2019-01-01 00:00:00

  • Predicting polymorphic EST-SSRs in silico.

    abstract::The public availability of large quantities of gene sequence data provides a valuable resource of the mining of Simple Sequence Repeat (SSR) molecular genetic markers for genetic analysis. These markers are inexpensive, require minimal labour to produce and can frequently be associated with functionally annotated gene...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12078

    authors: Duran C,Singhania R,Raman H,Batley J,Edwards D

    更新日期:2013-05-01 00:00:00

  • ednaoccupancy: An r package for multiscale occupancy modelling of environmental DNA data.

    abstract::In this article, we describe ednaoccupancy, an r package for fitting Bayesian, multiscale occupancy models. These models are appropriate for occupancy surveys that include three nested levels of sampling: primary sample units within a study area, secondary sample units collected from each primary unit and replicates o...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12735

    authors: Dorazio RM,Erickson RA

    更新日期:2018-03-01 00:00:00

  • Estimating mating rates in wild Drosophila melanogaster females by decay rates of male reproductive proteins in their reproductive tracts.

    abstract::Female Drosophila melanogaster frequently mate with multiple males in nature as shown through parentage analysis. Although polyandry is well documented, we know little about the timing between mating events in wild Drosophila populations due to the challenge of following behaviours of individual females. In this study...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12661

    authors: Giardina TJ,Clark AG,Fiumera AC

    更新日期:2017-11-01 00:00:00

  • New approaches to species delimitation and population structure of anthozoans: Two case studies of octocorals using ultraconserved elements and exons.

    abstract::As coral populations decline worldwide in the face of ongoing environmental change, documenting their distribution, diversity and conservation status is now more imperative than ever. Accurate delimitation and identification of species is a critical first step. This task, however, is not trivial as morphological varia...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.13241

    authors: Erickson KL,Pentico A,Quattrini AM,McFadden CS

    更新日期:2021-01-01 00:00:00

  • Real-time PCR to quantify composition of arbuscular mycorrhizal fungal communities--marker design, verification, calibration and field validation.

    abstract::Quantitative real-time PCR (qPCR) is slowly becoming established as a tool to quantify abundance of different arbuscular mycorrhizal fungal (AMF) taxa in roots and in soil. Here, we describe the development and field validation of qPCR markers (i.e. primers with associated hydrolysis probes), targeting taxon-specific ...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2011.03086.x

    authors: Thonar C,Erb A,Jansa J

    更新日期:2012-03-01 00:00:00

  • Microsatellite markers for mungbean developed from sequence database.

    abstract::A novel set of microsatellite markers for mungbean [Vigna radiata (L.) Wilczek] was developed from the public sequence database. Seventy-eight primers were designed and evaluated for polymorphism among 22 cultivated accessions. Eight polymorphic loci detected two to three alleles per locus with an average of 2.25. The...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2009.02655.x

    authors: Seehalak W,Somta P,Sommanas W,Srinives P

    更新日期:2009-05-01 00:00:00

  • Multiplex preamplification PCR and microsatellite validation enables accurate single nucleotide polymorphism genotyping of historical fish scales.

    abstract::Incorporating historical tissues into the study of ecological, conservation and management questions can broaden the scope of population genetic research by enhancing our understanding of evolutionary processes and anthropogenic influences on natural populations. Genotyping historical and low-quality samples has been ...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2010.02965.x

    authors: Smith MJ,Pascal CE,Grauvogel Z,Habicht C,Seeb JE,Seeb LW

    更新日期:2011-03-01 00:00:00

  • GONe: software for estimating effective population size in species with generational overlap.

    abstract::GONe is a user-friendly, Windows-based program for estimating effective size (N(e) ) in populations with overlapping generations. It uses the Jorde-Ryman modification to the temporal method to account for age structure in populations. This method requires estimates of age-specific survival and birth rate and allele fr...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2011.03057.x

    authors: Coombs JA,Letcher BH,Nislow KH

    更新日期:2012-01-01 00:00:00

  • Coherent synthesis of genomic associations with phenotypes and home environments.

    abstract::Local adaptation is often studied via (i) multiple common garden experiments comparing performance of genotypes in different environments and (ii) sequencing genotypes from multiple locations and characterizing geographic patterns in allele frequency. Both approaches aim to characterize the same pattern (local adaptat...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12714

    authors: Lasky JR,Forester BR,Reimherr M

    更新日期:2018-01-01 00:00:00

  • Applying DNA barcoding for the study of geographical variation in host-parasitoid interactions.

    abstract::Studies on the biogeography of host-parasitoid interactions are scarce, mainly because of technical difficulties associated with rearing and species identification. DNA barcoding is increasingly recognized as a valuable tool for taxon identification, allowing to link different life history stages of a species. We eval...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2010.02889.x

    authors: Santos AM,Besnard G,Quicke DL

    更新日期:2011-01-01 00:00:00

  • Chromosome-level assembly of wild Bactrian camel genome reveals organization of immune gene loci.

    abstract::Camelids are characterized by their unique adaptive immune system that exhibits the generation of homodimeric heavy-chain immunoglobulins, somatic hypermutation of T-cell receptors, and low genetic diversity of major histocompatibility complex (MHC) genes. However, short-read assemblies are typically highly fragmented...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.13141

    authors: Ming L,Wang Z,Yi L,Batmunkh M,Liu T,Siren D,He J,Juramt N,Jambl T,Li Y,Jirimutu

    更新日期:2020-05-01 00:00:00

  • Cross-species transferability of SSR loci developed from transciptome sequencing in lodgepole pine.

    abstract::With the advent of next generation sequencing technologies, transcriptome level sequence collections are arising as prominent resources for the discovery of gene-based molecular markers. In a previous study more than 15,000 simple sequence repeats (SSRs) in expressed sequence tag (EST) sequences resulting from 454 pyr...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2011.03102.x

    authors: Lesser MR,Parchman TL,Buerkle CA

    更新日期:2012-05-01 00:00:00

  • Expressed sequence tag-simple sequence repeats isolated from Shorea leprosula and their transferability to 36 species within the Dipterocarpaceae.

    abstract::Simple sequence repeats (SSRs) derived from expressed sequence tags (ESTs) are valuable markers because they represent transcribed regions and often transferable to related taxa. Here, we report the development and characterization of EST-SSRs from Shorea leprosula. Fifty-four sequences containing SSRs were identified...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2008.02238.x

    authors: Ng KK,Lee SL,Tsumura Y,Ueno S,Ng CH,Lee CT

    更新日期:2009-01-01 00:00:00

  • Isolation and characterization of microsatellite loci for Florida largemouth bass, Micropterus salmoides floridanus, and other micropterids.

    abstract::We isolated and characterized 52 novel microsatellite markers from Florida largemouth bass, Micropterus salmoides floridanus, for use in conservation, management and population genetic studies. Markers were assessed in M. s. floridanus from peninsular Florida (n = 30) and averaged eight alleles per locus with observed...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1471-8286.2007.01917.x

    authors: Lutz-Carrillo DJ,Hagen C,Dueck LA,Glenn TC

    更新日期:2008-01-01 00:00:00

  • DNA barcodes from century-old type specimens using next-generation sequencing.

    abstract::Type specimens have high scientific importance because they provide the only certain connection between the application of a Linnean name and a physical specimen. Many other individuals may have been identified as a particular species, but their linkage to the taxon concept is inferential. Because type specimens are o...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12474

    authors: Prosser SW,deWaard JR,Miller SE,Hebert PD

    更新日期:2016-03-01 00:00:00

  • Microsatellite loci for dreissenid mussels (Mollusca: Bivalvia: Dreissenidae) and relatives: markers for assessing exotic and native populations.

    abstract::We developed and tested 14 new polymorphic microsatellite loci for dreissenid mussels, including the two species that have invaded many freshwater habitats in Eurasia and North America, where they cause serious industrial fouling damage and ecological alterations. These new loci will aid our understanding of their gen...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2011.03012.x

    authors: Feldheim KA,Brown JE,Murphy DJ,Stepien CA

    更新日期:2011-07-01 00:00:00

  • Identification and characterization of microsatellite loci in Intsia palembanica (Leguminosae), a valuable tropical timber species.

    abstract::We present 25 polymorphic microsatellite loci for Intsia palembanica, a highly valued timber species in the Indo-Malayan region, Australia and western Pacific islands. Microsatellite loci were tested for polymorphism across a total of 76 individuals from three natural populations from Irian Jaya, Indonesia. The averag...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2008.02491.x

    authors: Wong KN,Tan WL,Chew FT

    更新日期:2009-01-01 00:00:00

  • Development of simple sequence repeat markers for the soybean rust fungus, Phakopsora pachyrhizi.

    abstract::Twenty-four simple sequence repeat markers were developed for Phakopsora pachyrhizi, a fungal pathogen of soybean (Glycine max) and other legumes. All 24 of the loci were evaluated on 28 isolates of P. pachyrhizi. Twenty-one loci were polymorphic, with allelic diversity ranging from two to eight alleles, and null alle...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2008.02272.x

    authors: Anderson SJ,Stone CL,Posada-Buitrago ML,Boore JL,Neelam BA,Stephens RM,Luster DG,Frederick RD,Pedley KF

    更新日期:2008-11-01 00:00:00

  • Modelling cross-hybridization on phylogenetic DNA microarrays increases the detection power of closely related species.

    abstract::DNA microarrays are a popular technique for the detection of microorganisms. Several approaches using specific oligomers targeting one or a few marker genes for each species have been proposed. Data analysis is usually limited to call a species present when its oligomer exceeds a certain intensity threshold. While thi...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2008.02199.x

    authors: Engelmann JC,Rahmann S,Wolf M,Schultz J,Fritzilas E,Kneitz S,Dandekar T,Müller T

    更新日期:2009-01-01 00:00:00

  • De novo assembly and characterization of leaf and floral transcriptomes of the hybridizing bromeliad species (Pitcairnia spp.) adapted to Neotropical Inselbergs.

    abstract::We present the leaf and floral transcriptomes of two hybridizing bromeliad species that differ in their major pollinator systems. Here we identified candidate genes responsible for pollinator attraction and reproductive isolation in these two species. We searched for candidate genes involved in floral traits, such as ...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12504

    authors: Palma-Silva C,Ferro M,Bacci M,Turchetto-Zolet AC

    更新日期:2016-07-01 00:00:00

  • A chromosome-level genome assembly of rice leaffolder, Cnaphalocrocis medinalis.

    abstract::The rice leaffolder, Cnaphalocrocis medinalis Guenée (Crambidae, Lepidoptera), is an important agricultural pest that causes serious losses to rice production in rice-growing regions with high humidity and temperature. However, a lack of genomic resources limits in-depth understanding of its biological characteristics...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.13274

    authors: Zhao X,Xu H,He K,Shi Z,Chen X,Ye X,Mei Y,Yang Y,Li M,Gao L,Xu L,Xiao H,Liu Y,Lu Z,Li F

    更新日期:2021-02-01 00:00:00

  • Development of microsatellite markers in Cordia bifurcata (Boraginaceae) and cross-species amplification in Cordia inermis and Cordia pringlei.

    abstract::We developed 16 microsatellite markers in Cordia bifurcata, a Central and South American shrub. The markers show low polymorphism in C. bifurcata, a species suspected of self-fertilization or apomixis. Of four polymorphic loci, three had only two alleles. However, current research indicates that these markers hold val...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2008.02131.x

    authors: Spoon TR,Kesseli RV

    更新日期:2008-09-01 00:00:00

  • A first step towards inferring levels of long-distance dispersal during past expansions.

    abstract::Improving the realism of spatially explicit demographic models is important for better inferring the history of past populations and for understanding the genetic bases of adaptation and speciation. One particular type of demographic event to take into account is long-distance dispersal (LDD). The goals of this study ...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2010.02881.x

    authors: Ray N,Excoffier L

    更新日期:2010-09-01 00:00:00

  • Analysing diversity and community structures using PCR-RFLP: a new software application.

    abstract::Restriction fragment length polymorphism tools is an R application which supports a complete workflow of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), dealing with the problems which accompany analysis when PCR-RFLP is used in diversity studies. Large numbers of different RFLP samples ...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12094

    authors: Flessa F,Kehl A,Kohl M

    更新日期:2013-07-01 00:00:00

  • Development and characterization of eight polymorphic microsatellite markers for Daphnia atkinsoni (Crustacea: Ctenodaphnia).

    abstract::A microsatellite-enriched genomic library was developed for the water flea Daphnia atkinsoni Baird, 1859, a dominant species of intermittent wetlands in Europe. Eight polymorphic microsatellite markers were successfully optimized. Characterization of 77 individuals from Belgium and Spain showed moderate (in the former...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2008.02375.x

    authors: Ortells R,VAN Houdt JK,Geldof S,DE Meester L,Mergeay J

    更新日期:2009-01-01 00:00:00

  • A dedicated target capture approach reveals variable genetic markers across micro- and macro-evolutionary time scales in palms.

    abstract::Understanding the genetics of biological diversification across micro- and macro-evolutionary time scales is a vibrant field of research for molecular ecologists as rapid advances in sequencing technologies promise to overcome former limitations. In palms, an emblematic, economically and ecologically important plant f...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12945

    authors: de La Harpe M,Hess J,Loiseau O,Salamin N,Lexer C,Paris M

    更新日期:2019-01-01 00:00:00