Abstract:
:Increasing numbers of phylogeographic studies make comparative inferences about the histories of co-distributed species. Although the aims of such studies are best achieved by jointly analysing sequences from multiple loci in a model-based framework, such data currently exist for few nonmodel systems. We used existing genomic data and expressed sequence tags (ESTs) for Hymenoptera and other insects to design intron-crossing primers for 40 loci, mainly ribosomal proteins, for chalcidoid parasitoids. Amplification success was scored on a range of taxa associated with two natural communities; oak galls and figs. Taxa were chosen at increasing distance from Nasonia, which was used for primer design, (i) within Pteromalids, (ii) within Chalcidoidea (Eupelmidae, Eulophidae, Eurytomidae, Ormyridae, Torymidae) and (iii) for a selection of distantly related gall and fig wasps (Cynipidae, Agaonidae). To assess the utility of these loci for phylogeographic and population genetic studies, we compared genetic diversity between Western Palaearctic refugia for two species. Our results show that it is feasible to design a large number of exon-primed-intron-crossing (EPIC) loci that may be informative about phylogeographic history within species but amplify across a large taxonomic range.
journal_name
Mol Ecol Resourjournal_title
Molecular ecology resourcesauthors
Lohse K,Sharanowski B,Blaxter M,Nicholls JA,Stone GNdoi
10.1111/j.1755-0998.2010.02956.xsubject
Has Abstractpub_date
2011-05-01 00:00:00pages
521-9issue
3eissn
1755-098Xissn
1755-0998journal_volume
11pub_type
杂志文章abstract::Restriction-site-associated DNA sequencing (RAD-seq) and related methods are revolutionizing the field of population genomics in nonmodel organisms as they allow generating an unprecedented number of single nucleotide polymorphisms (SNPs) even when no genomic information is available. Yet, RAD-seq data analyses rely o...
journal_title:Molecular ecology resources
pub_type: 杂志文章
doi:10.1111/1755-0998.12518
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abstract::Degraded DNA from suboptimal field sampling is common in molecular ecology. However, its impact on techniques that use restriction site associated next-generation DNA sequencing (RADSeq, GBS) is unknown. We experimentally examined the effects of in situDNA degradation on data generation for a modified double-digest RA...
journal_title:Molecular ecology resources
pub_type: 杂志文章
doi:10.1111/1755-0998.12404
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abstract::Museum collections are essential for reconstructing and understanding past biodiversity. Many museum specimens are, however, challenging to identify. Museum samples may be incomplete, have an unusual morphology, or represent juvenile individuals, all of which complicate accurate identification. In some cases, inaccura...
journal_title:Molecular ecology resources
pub_type: 杂志文章
doi:10.1111/1755-0998.13130
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journal_title:Molecular ecology resources
pub_type: 评论,信件
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journal_title:Molecular ecology resources
pub_type: 杂志文章
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journal_title:Molecular ecology resources
pub_type: 杂志文章
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journal_title:Molecular ecology resources
pub_type: 杂志文章
doi:10.1111/1755-0998.12303
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journal_title:Molecular ecology resources
pub_type: 杂志文章
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abstract::Crepidula convexa, a calyptreid gastropod with direct embryonic development, changes sex from male to female in the course of its lifetime (protandry). Under sex-allocation theory, male reproductive success should be independent from age and size (a proxy used for age). However, this may be counterbalanced by female c...
journal_title:Molecular ecology resources
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Molecular ecology resources
pub_type: 杂志文章
doi:10.1111/j.1755-0998.2008.02099.x
更新日期:2008-07-01 00:00:00
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journal_title:Molecular ecology resources
pub_type: 杂志文章
doi:10.1111/1755-0998.12210
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pub_type: 杂志文章
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journal_title:Molecular ecology resources
pub_type: 杂志文章
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journal_title:Molecular ecology resources
pub_type: 杂志文章
doi:10.1111/j.1755-0998.2008.02328.x
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journal_title:Molecular ecology resources
pub_type: 杂志文章,评审
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更新日期:2011-05-01 00:00:00
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journal_title:Molecular ecology resources
pub_type: 杂志文章
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更新日期:2019-03-01 00:00:00
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journal_title:Molecular ecology resources
pub_type: 杂志文章
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更新日期:2009-05-01 00:00:00
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journal_title:Molecular ecology resources
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Molecular ecology resources
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Molecular ecology resources
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journal_title:Molecular ecology resources
pub_type: 杂志文章
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journal_title:Molecular ecology resources
pub_type: 杂志文章,评审
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journal_title:Molecular ecology resources
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更新日期:2009-01-01 00:00:00
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journal_title:Molecular ecology resources
pub_type: 杂志文章
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