Impacts of degraded DNA on restriction enzyme associated DNA sequencing (RADSeq).

abstract：:Ecological understanding of the role of consumer-resource interactions in natural food webs is limited by the difficulty of accurately and efficiently determining the complex variety of food types animals have eaten in the field. We developed a method based on DNA metabarcoding multiplexing and next-generation sequenc...

journal_title：Molecular ecology resources

authors： De Barba M,Miquel C,Boyer F,Mercier C,Rioux D,Coissac E,Taberlet P

更新日期：2014-03-01 00:00:00

abstract：:This article documents the public availability of raw transcriptome sequence data and 63,020 SNPs for the polar bear (Ursus maritimus). ...

journal_title：Molecular ecology resources

authors： Genomic Resources Development Consortium.,Coltman DW,Davis CS,Lunn NJ,Malenfant RM,Richardson ES

更新日期：2014-01-01 00:00:00

abstract：:sam is a Windows program designed to detect candidate loci for selection in whole-genome scans. It also gives valuable clues as regards the ecological factors at stake in the selection process. The method used is based on multiple univariate logistic regression models to test for association between allelic frequencie...

journal_title：Molecular ecology resources

authors： Joost S,Kalbermatten M,Bonin A

更新日期：2008-09-01 00:00:00

abstract：:Restriction-site-associated DNA tag (RAD-tag) sequencing has become a popular approach to generate thousands of SNPs used to address diverse questions in population genomics. Comparatively, the suitability of RAD-tag genotyping to address evolutionary questions across divergent species has been the subject of only a f...

journal_title：Molecular ecology resources

authors： Viricel A,Pante E,Dabin W,Simon-Bouhet B

更新日期：2014-05-01 00:00:00

abstract：:Data on hundreds or thousands of single nucleotide polymorphisms (SNPs) provide detailed information about the relationships between individuals, but currently few tools can turn this information into a multigenerational pedigree. I present the r package sequoia, which assigns parents, clusters half-siblings sharing a...

journal_title：Molecular ecology resources

authors： Huisman J

更新日期：2017-09-01 00:00:00

abstract：:Reliable identification of Aphidiinae species (Braconidae) is a prerequisite for conducting studies on aphid-parasitoid interactions at the community level. However, morphological identification of Aphidiinae species remains problematic even for specialists and is almost impossible with larval stages. Here, we compare...

journal_title：Molecular ecology resources

authors： Derocles SA,LE Ralec A,Plantegenest M,Chaubet B,Cruaud C,Cruaud A,Rasplus JY

更新日期：2012-03-01 00:00:00

abstract：:Nematodes play an important role in ecosystem processes, yet the relevance of nematode species diversity to ecology is unknown. Because nematode identification of all individuals at the species level using standard techniques is difficult and time-consuming, nematode communities are not resolved down to the species le...

journal_title：Molecular ecology resources

authors： Porazinska DL,Giblin-Davis RM,Faller L,Farmerie W,Kanzaki N,Morris K,Powers TO,Tucker AE,Sung W,Thomas WK

更新日期：2009-11-01 00:00:00

abstract：:Many plants and some animal species are polyploids. Nondisomically inherited markers (e.g. microsatellites) in such species cannot be analysed directly by standard population genetics methods developed for diploid species. One solution is to transform the polyploid codominant genotypes to pseudodiploid-dominant genoty...

journal_title：Molecular ecology resources

authors： Wang J,Scribner KT

更新日期：2014-05-01 00:00:00

abstract：:A novel set of microsatellite markers for mungbean [Vigna radiata (L.) Wilczek] was developed from the public sequence database. Seventy-eight primers were designed and evaluated for polymorphism among 22 cultivated accessions. Eight polymorphic loci detected two to three alleles per locus with an average of 2.25. The...

journal_title：Molecular ecology resources

authors： Seehalak W,Somta P,Sommanas W,Srinives P

更新日期：2009-05-01 00:00:00

abstract：:Consensus primers, based on exon sequences of the cyp73 gene family coding for cinnamate 4-hydroxylase (C4H) of the lignin biosynthesis pathway, were designed for the tetraploid willow species Salix alba and Salix fragilis. Diagnostic alleles at species level were observed among introns of three cyp73 genes and allowe...

journal_title：Molecular ecology resources

authors： Trung le Q,VAN Puyvelde K,Triest L

更新日期：2008-03-01 00:00:00

abstract：:Stegodyphus lineatus spiders live in groups consisting of closely related individuals. There appears to be no discrimination against related individuals as mates but females mate multiply, despite the fact that matings are shown to carry a cost. We have developed eight polymorphic dinucleotide microsatellite markers t...

journal_title：Molecular ecology resources

authors： Bilde T,Tuni C,Cariani A,Santini A,Tabarroni C,Garoia F,Goodacre SL

更新日期：2009-01-01 00:00:00

abstract：:We describe primers and polymerase chain reaction conditions to amplify 22 microsatellite loci from the Barrow's goldeneye (Bucephala islandica). The primers were tested on 27 individuals from a population breeding in British Columbia, Canada. The developed primer pairs yielded an average of 6.11 alleles per locus (ra...

journal_title：Molecular ecology resources

authors： Jaari S,Jaatinen K,Merilä J

更新日期：2009-05-01 00:00:00

abstract：:As coral populations decline worldwide in the face of ongoing environmental change, documenting their distribution, diversity and conservation status is now more imperative than ever. Accurate delimitation and identification of species is a critical first step. This task, however, is not trivial as morphological varia...

journal_title：Molecular ecology resources

authors： Erickson KL,Pentico A,Quattrini AM,McFadden CS

更新日期：2021-01-01 00:00:00

abstract：:Biodiversity analyses based on next-generation sequencing (NGS) platforms have developed by leaps and bounds in recent years. A PCR-free strategy, which can alleviate taxonomic bias, was considered as a promising approach to delivering reliable species compositions of targeted environments. The major impediment of suc...

journal_title：Molecular ecology resources

authors： Liu S,Wang X,Xie L,Tan M,Li Z,Su X,Zhang H,Misof B,Kjer KM,Tang M,Niehuis O,Jiang H,Zhou X

更新日期：2016-03-01 00:00:00

abstract：:The spatial signature of microevolutionary processes structuring genetic variation may play an important role in the detection of loci under selection. However, the spatial location of samples has not yet been used to quantify this. Here, we present a new two-step method of spatial outlier detection at the individual ...

journal_title：Molecular ecology resources

authors： Wagner HH,Chávez-Pesqueira M,Forester BR

更新日期：2017-11-01 00:00:00

abstract：:Determining the origin of individuals in mixed population samples is key in many ecological, conservation and management contexts. Genetic data can be analyzed using genetic stock identification (GSI), where the origin of single individuals is determined using Individual Assignment (IA) and population proportions are ...

journal_title：Molecular ecology resources

authors： Östergren J,Palm S,Gilbey J,Dannewitz J

更新日期：2020-03-01 00:00:00

abstract：:The soil microbiome is inherently complex with high biological diversity, and spatial heterogeneity typically occurring on the submillimetre scale. To study the microbial ecology of soils, and other microbiomes, biomolecules, that is, nucleic acids and proteins, must be efficiently and reliably co-recovered from the s...

journal_title：Molecular ecology resources

authors： Thorn CE,Bergesch C,Joyce A,Sambrano G,McDonnell K,Brennan F,Heyer R,Benndorf D,Abram F

更新日期：2019-03-01 00:00:00

abstract：:Thirty-eight microsatellite loci were developed for the bird pollinated, autohexaploid, Eremophila glabra ssp. glabra. A genomic library was screened with dinucleotide and trinucleotide sequence repeats. Polymorphism ranged from one to 21 alleles per locus. Twenty-four loci exhibited null alleles, based on patterns of...

journal_title：Molecular ecology resources

authors： Elliott CP

更新日期：2009-07-01 00:00:00

abstract：:This article documents the public availability of approximately 15 000 polymorphic SNP loci for the bighorn sheep Ovis canadensis. ...

journal_title：Molecular ecology resources

authors： Genomic Resources Development Consortium.,Coltman DW,Hogg JT,Miller JM

更新日期：2013-09-01 00:00:00

abstract：:DNA microarrays are a popular technique for the detection of microorganisms. Several approaches using specific oligomers targeting one or a few marker genes for each species have been proposed. Data analysis is usually limited to call a species present when its oligomer exceeds a certain intensity threshold. While thi...

journal_title：Molecular ecology resources

authors： Engelmann JC,Rahmann S,Wolf M,Schultz J,Fritzilas E,Kneitz S,Dandekar T,Müller T

更新日期：2009-01-01 00:00:00

abstract：:Oestrogenic wastewater treatment works (WwTW) effluents discharged into UK rivers have been shown to affect sexual development, including inducing intersex, in wild roach (Rutilus rutilus). This can result in a reduced breeding capability with potential population level impacts. In the absence of a sex probe for roach...

journal_title：Molecular ecology resources

authors： Lange A,Paris JR,Gharbi K,Cézard T,Miyagawa S,Iguchi T,Studholme DJ,Tyler CR

更新日期：2020-07-01 00:00:00

abstract：:The development of genetic markers has revolutionized molecular studies within and among populations. Although poly-allelic microsatellites are the most commonly used genetic marker for within-population studies of free-living animals, biallelic single nucleotide polymorphisms, or SNPs, have also emerged as a viable o...

journal_title：Molecular ecology resources

authors： Weinman LR,Solomon JW,Rubenstein DR

更新日期：2015-05-01 00:00:00

abstract：:Publilia concava is an eastern North American membracid commonly occurring in large but spatially patchy aggregations, primarily on the host plant Solidago altissima. Like other myrmecophiles, P. concava provides sugary excretions to ants in return for the various protective, competitive or even sanitary benefits that...

journal_title：Molecular ecology resources

authors： Chhatre V,Morales MA,Abbot P

更新日期：2009-07-01 00:00:00

abstract：:Molecular markers have been used to identify the sex of sampled individuals for several decades, but the time-consuming development phase prevented their application in many systems. Recently, a growing number of papers have applied reduced-representation sequencing (RRS) protocols to the identification of sex-specifi...

journal_title：Molecular ecology resources

authors： Moore JS,Benestan L

更新日期：2018-03-01 00:00:00

abstract：:This study examined the interspecific amplification of nuclear microsatellite loci developed mainly for eucalypts in the subgenus Symphyomyrtus across five species within the second most speciose subgenus, subgenus Eucalyptus. A set of eight to 10 loci, depending on taxon, have been identified that are highly variable...

journal_title：Molecular ecology resources

authors： Nevill PG,Reed A,Bossinger G,Vaillancourt RE,Larcombe M,Ades PK

更新日期：2008-11-01 00:00:00

abstract：:Eight polymorphic microsatellites were isolated from the Greek smooth newt (Lissotriton vulgaris graecus) using a microsatellite enrichment protocol and selective hybridization with a biotinylated (AC)(11) probe. The loci showed different variation patterns in a single breeding population (32 individuals) with mean nu...

journal_title：Molecular ecology resources

authors： Sotiropoulos K,Eleftherakos K,Tsaparis D,Kasapidis P,Magoulas A,Legakis A

更新日期：2009-01-01 00:00:00

abstract：:Genetic stock identification (GSI) is an important tool in fisheries management. Microsatellites (μSATs) have been the dominant genetic marker for GSI; however, increasing availability and numerous advantages of single-nucleotide polymorphism (SNP) markers make them an appealing alternative. We tested performance of 1...

journal_title：Molecular ecology resources

authors： Hess JE,Matala AP,Narum SR

更新日期：2011-03-01 00:00:00

abstract：:We present 25 polymorphic microsatellite loci for Intsia palembanica, a highly valued timber species in the Indo-Malayan region, Australia and western Pacific islands. Microsatellite loci were tested for polymorphism across a total of 76 individuals from three natural populations from Irian Jaya, Indonesia. The averag...

journal_title：Molecular ecology resources

authors： Wong KN,Tan WL,Chew FT

更新日期：2009-01-01 00:00:00

abstract：:In order to facilitate the addition of a genetic component to conservation management plans for Georgia false indigo (Amorpha georgiana var. georgiana), a rare legume of the southeastern USA, 12 polymorphic microsatellite markers were developed. No gametic disequilibrium was detected among locus pairs, but observation...

journal_title：Molecular ecology resources

authors： Straub SC,Bogdanowicz SM,Doyle JJ

更新日期：2009-01-01 00:00:00

abstract：:The concept of effective population size (N(e) ) is based on an elegantly simple idea which, however, rapidly becomes very complex when applied to most real-world situations. In natural populations, spatial and temporal stratifications create different classes of individuals with different vital rates, and this in tur...

journal_title：Molecular ecology resources

authors： Waples RS

更新日期：2010-09-01 00:00:00