Identification of molecular markers for DNA barcoding in the Aphidiinae (Hym. Braconidae).

abstract：:Despite the contribution of DNA barcoding towards understanding the biodiversity and distribution of species, the success of COI amplification has been quite variable when it comes to freshwater zooplankton (Elías-Gutiérrez & Valdez-Moreno 2008; Jeffery et al. 2011). Some genera of microcrustaceans seem to be more dif...

journal_title：Molecular ecology resources

authors： Prosser S,Martínez-Arce A,Elías-Gutiérrez M

更新日期：2013-11-01 00:00:00

abstract：:DNA microarrays are a popular technique for the detection of microorganisms. Several approaches using specific oligomers targeting one or a few marker genes for each species have been proposed. Data analysis is usually limited to call a species present when its oligomer exceeds a certain intensity threshold. While thi...

journal_title：Molecular ecology resources

authors： Engelmann JC,Rahmann S,Wolf M,Schultz J,Fritzilas E,Kneitz S,Dandekar T,Müller T

更新日期：2009-01-01 00:00:00

abstract：:The three-toed sloths (Bradypus) are slow-moving arboreal neotropical mammals. Understanding demographic variables (such as sex ratio) of populations is a key for conservation purposes. Nevertheless, gender assignment of Bradypus is particularly challenging because of the lack of sexual dimorphism in infants and in ad...

journal_title：Molecular ecology resources

authors： Martinelli AB,DE Moraes-Barros N,Alvarenga CS,Chaves PB,Santos LA,Fagundes V

更新日期：2010-07-01 00:00:00

abstract：:Global climate change (GCC) may be causing distribution range shifts in many organisms worldwide. Multiple efforts are currently focused on the development of models to better predict distribution range shifts due to GCC. We addressed this issue by including intraspecific genetic structure and spatial autocorrelation ...

journal_title：Molecular ecology resources

authors： Martínez-Minaya J,Conesa D,Fortin MJ,Alonso-Blanco C,Picó FX,Marcer A

更新日期：2019-07-01 00:00:00

abstract：:Germplasm collections provide an extremely valuable resource for breeders and researchers. However, misclassification of accessions by species often hinders the effective use of these collections. We propose that use of high-throughput genotyping tools can provide a fast, efficient and cost-effective way of confirming...

journal_title：Molecular ecology resources

authors： Mason AS,Zhang J,Tollenaere R,Vasquez Teuber P,Dalton-Morgan J,Hu L,Yan G,Edwards D,Redden R,Batley J

更新日期：2015-09-01 00:00:00

abstract：:Noninvasive genetic sampling has increasingly been used in ecological and conservation studies during the last decade. A major part of the noninvasive genetic literature is dedicated to the search for optimal protocols, by comparing different methods of collection, preservation and extraction of DNA from noninvasive m...

journal_title：Molecular ecology resources

authors： Renan S,Speyer E,Shahar N,Gueta T,Templeton AR,Bar-David S

更新日期：2012-11-01 00:00:00

abstract：:Fifteen polymorphic markers were developed from a microsatellite-enriched library for the lizard Podarcis gaigeae. The loci were checked for variability in 68 individuals from a population on the island of Skyros, Greece. The number of alleles ranged from 3 to 23 per locus and expected heterozygosity from 0.29 and 0.9...

journal_title：Molecular ecology resources

authors： Wellenreuther M,Runemark A,Svensson EI,Hansson B

更新日期：2009-05-01 00:00:00

abstract：:Sex-determining modes remain unknown in numerous species, notably in fishes, in which a variety of modalities have been reported. Additionally, noninvasive individual sexing is problematic for species without external sex attributes or for early life stages, requiring cytogenetic or molecular analyses when sex chromos...

journal_title：Molecular ecology resources

authors： Trenkel VM,Boudry P,Verrez-Bagnis V,Lorance P

更新日期：2020-11-01 00:00:00

abstract：:Pedigree reconstruction using genotypic markers has become an important tool for the study of natural populations. The nonstandard nature of the underlying statistical problems has led to the necessity of developing specialized statistical and computational methods. In this article, a new version of pedigree reconstru...

journal_title：Molecular ecology resources

authors： Almudevar A,Anderson EC

更新日期：2012-01-01 00:00:00

abstract：:Nematodes play an important role in ecosystem processes, yet the relevance of nematode species diversity to ecology is unknown. Because nematode identification of all individuals at the species level using standard techniques is difficult and time-consuming, nematode communities are not resolved down to the species le...

journal_title：Molecular ecology resources

authors： Porazinska DL,Giblin-Davis RM,Faller L,Farmerie W,Kanzaki N,Morris K,Powers TO,Tucker AE,Sung W,Thomas WK

更新日期：2009-11-01 00:00:00

abstract：:We combined restriction site associated DNA sequencing (RADseq) using a hypomethylation-sensitive enzyme and messenger RNA sequencing (mRNAseq) to develop molecular markers for the 16 gigabase genome of Cedrus atlantica, a conifer tree species. With each method, Illumina(®) reads from one individual were used to gener...

journal_title：Molecular ecology resources

authors： Karam MJ,Lefèvre F,Dagher-Kharrat MB,Pinosio S,Vendramin GG

更新日期：2015-05-01 00:00:00

abstract：:Thirty-four novel microsatellite markers developed for wood cricket (Nemobius sylvestris) were tested and optimized. Twenty-five microsatellite loci were polymorphic, exhibiting between two and nine alleles. Observed heterozygosities ranged from 0.038 to 0.925. The microsatellites were also tested in a species belongi...

journal_title：Molecular ecology resources

authors： Vanhala T,Cottrell J

更新日期：2008-11-01 00:00:00

abstract：:A multiplex haplotype-specific polymerase chain reaction (MHS-PCR) method was developed, which identified seven Clupeiform species living in the tropical Eastern Atlantic region: Sardinella aurita, Sardinella maderensis, Ethmalosa fimbriata, Sardina pilchardus, Engraulis encrasicholus, Pellonula leonensis and Ilisha a...

journal_title：Molecular ecology resources

authors： Durand JD,Diatta MA,Diop K,Trape S

更新日期：2010-05-01 00:00:00

abstract：:Medicago species are important genetic sources for forage crops and nitrogen sources for various ecosystems. The ongoing genome sequencing of the model legume, Medicago truncatula, provides a wealth of genetic markers potentially useful for characterizing the population genetic structure and evolutionary history, and ...

journal_title：Molecular ecology resources

authors： Chu HJ,Yan J,Hu Y,Wang HC,Li JQ

更新日期：2010-01-01 00:00:00

abstract：:DNA metabarcoding was utilized for a large-scale, multiyear assessment of biodiversity in Malaise trap collections from the Bavarian Forest National Park (Germany, Bavaria). Principal component analysis of read count-based biodiversities revealed clustering in concordance with whether collection sites were located ins...

journal_title：Molecular ecology resources

authors： Hardulak LA,Morinière J,Hausmann A,Hendrich L,Schmidt S,Doczkal D,Müller J,Hebert PDN,Haszprunar G

更新日期：2020-11-01 00:00:00

abstract：:Rhizoprionodon lalandii and R. porosus are widely distributed along the Atlantic coast of the Americas, living close to coastal areas and therefore frequently captured by seaboard fisheries. However, morphological identification of species in this genus is very difficult, especially when sharks have their heads and fi...

journal_title：Molecular ecology resources

authors： Mendonça FF,Hashimoto DT,Porto-Foresti F,Oliveira C,Gadig OB,Foresti F

更新日期：2009-05-01 00:00:00

abstract：:Collectively called zooxanthellae, photosynthetic dinoflagellates in the family Symbiodiniaceae are typical endosymbionts that unequivocally mediate coral responses to environmental changes. Symbiodiniaceae are genetically diverse, encompassing at least nine phylogenetically distinct genera (clades A-I). The ribosomal...

journal_title：Molecular ecology resources

authors： Shi T,Niu G,Kvitt H,Zheng X,Qin Q,Sun D,Ji Z,Tchernov D

更新日期：2021-01-01 00:00:00

abstract：:We isolated and characterized 52 novel microsatellite markers from Florida largemouth bass, Micropterus salmoides floridanus, for use in conservation, management and population genetic studies. Markers were assessed in M. s. floridanus from peninsular Florida (n = 30) and averaged eight alleles per locus with observed...

journal_title：Molecular ecology resources

authors： Lutz-Carrillo DJ,Hagen C,Dueck LA,Glenn TC

更新日期：2008-01-01 00:00:00

abstract：:DNA barcoding is a global initiative that provides a standardized and efficient tool to catalogue and inventory biodiversity, with significant conservation applications. Despite progress across taxonomic realms, globally threatened marine turtles remain underrepresented in this effort. To obtain DNA barcodes of marine...

journal_title：Molecular ecology resources

authors： Naro-Maciel E,Reid B,Fitzsimmons NN,LE M,Desalle R,Amato G

更新日期：2010-03-01 00:00:00

abstract：:In noninvasive genetic sampling, when genotyping error rates are high and recapture rates are low, misidentification of individuals can lead to overestimation of population size. Thus, estimating genotyping errors is imperative. Nonetheless, conducting multiple polymerase chain reactions (PCRs) at multiple loci is tim...

journal_title：Molecular ecology resources

authors： Hansen H,Ben-David M,McDonald DB

更新日期：2008-03-01 00:00:00

abstract：:Twelve polymorphic microsatellite loci were isolated for the bonefish, Albula vulpes using a polymerase chain reaction-based procedure. The number of alleles ranged from two to 23 (mean = 8.8) in 37 specimens from south Florida. Observed and expected heterozygosities ranged from 0.07 to 0.77 (mean = 0.42) and from 0.0...

journal_title：Molecular ecology resources

authors： Seyoum S,Wallace EM,Tringali MD

更新日期：2008-03-01 00:00:00

abstract：:Microsatellite markers were developed to assess population structure and patterns of translocation in the introduced European common genet (Genetta genetta). Primer pairs were designed for 60 microsatellite sequences enriched for CA, GA, CATC and TAGA repeat motifs. Eleven loci that proved to be polymorphic were genot...

journal_title：Molecular ecology resources

authors： Gaubert P,Del Cerro I,Palomares F,Godoy JA

更新日期：2008-11-01 00:00:00

abstract：:Optimal integration of next-generation sequencing into mainstream research requires re-evaluation of how problems can be reasonably overcome and what questions can be asked. One potential application is the rapid acquisition of genomic information to identify microsatellite loci for evolutionary, population genetic an...

journal_title：Molecular ecology resources

authors： Castoe TA,Poole AW,Gu W,Jason de Koning AP,Daza JM,Smith EN,Pollock DD

更新日期：2010-03-01 00:00:00

abstract：:Inferring the mode and tempo of natural selection helps further our understanding of adaptation to past environmental changes. Here, we introduce McSwan, a method to detect and date past and recent natural selection events in the case of a hard sweep. The method is based on the comparison of site frequency spectra obt...

journal_title：Molecular ecology resources

authors： Tournebize R,Poncet V,Jakobsson M,Vigouroux Y,Manel S

更新日期：2019-01-01 00:00:00

abstract：:Here, we describe Assemble Species by Automatic Partitioning (ASAP), a new method to build species partitions from single locus sequence alignments (i.e., barcode data sets). ASAP is efficient enough to split data sets as large 104 sequences into putative species in several minutes. Although grounded in evolutionary t...

journal_title：Molecular ecology resources

authors： Puillandre N,Brouillet S,Achaz G

更新日期：2021-02-01 00:00:00

abstract：:In this study, we reported the isolation and analysis of new polymorphic microsatellites in mungbean (Vigna radiata (L.) Wilczek). Twelve out of 210 primer pairs screened in 30 mungbean accessions gave polymorphism. The polymorphic markers detected two to three alleles per locus with an average of 2.08. Observed heter...

journal_title：Molecular ecology resources

authors： Somta P,Musch W,Kongsamai B,Chanprame S,Nakasathien S,Toojinda T,Sorajjapinun W,Seehalak W,Tragoonrung S,Srinives P

更新日期：2008-09-01 00:00:00

abstract：:DNA-based identifications have been employed across broad taxonomic ranges and provide an especially useful tool in cases where external identification may be problematic. This study explored the utility of DNA barcoding in resolving skate species found in Atlantic Canadian waters. Most species were clearly resolved, ...

journal_title：Molecular ecology resources

authors： Coulson MW,Denti D,Van Guelpen L,Miri C,Kenchington E,Bentzen P

更新日期：2011-11-01 00:00:00

abstract：:The use of dominant markers such as amplified fragment length polymorphism (AFLP) for population genetics analyses is often impeded by the lack of appropriate computer programs and rarely motivated by objective considerations. The point of the present note is twofold: (i) we describe how the computer program Geneland ...

journal_title：Molecular ecology resources

authors： Guillot G,Santos F

更新日期：2010-11-01 00:00:00

abstract：:Avian malaria and related haemosporidians (Plasmodium, [Para]Haemoproteus and Leucocytoozoon) represent an exciting multihost, multiparasite system in ecology and evolution. Global research in this field accelerated after the publication in 2000 of PCR protocols to sequence a haemosporidian mitochondrial (mtDNA) barco...

journal_title：Molecular ecology resources

authors： Barrow LN,Allen JM,Huang X,Bensch S,Witt CC

更新日期：2019-03-01 00:00:00

abstract：:This article documents the public availability of raw transcriptome sequence data and 63,020 SNPs for the polar bear (Ursus maritimus). ...

journal_title：Molecular ecology resources

authors： Genomic Resources Development Consortium.,Coltman DW,Davis CS,Lunn NJ,Malenfant RM,Richardson ES

更新日期：2014-01-01 00:00:00