Molecular methods for arthropod bloodmeal identification and applications to ecological and vector-borne disease studies.

abstract：:Genomic data sets are increasingly central to ecological and evolutionary biology, but far fewer resources are available for invertebrates. Powerful new computational tools and the rapidly decreasing cost of Illumina sequencing are beginning to change this, enabling rapid genome assembly and reference marker extractio...

journal_title：Molecular ecology resources

authors： Sun X,Ding Y,Orr MC,Zhang F

更新日期：2020-05-01 00:00:00

abstract：:Microsatellites are valuable markers for the analysis of genetic diversity, linkage mapping or genotyping. The limited availability of microsatellites for the genus Potentilla (Rosaceae) stipulated the isolation of markers from a representative (Potentilla pusilla Host) of the Potentilla core group that constitutes th...

journal_title：Molecular ecology resources

authors： Dobeš Ch,Scheffknecht S

更新日期：2012-07-01 00:00:00

abstract：:DNA barcoding is a global initiative that provides a standardized and efficient tool to catalogue and inventory biodiversity, with significant conservation applications. Despite progress across taxonomic realms, globally threatened marine turtles remain underrepresented in this effort. To obtain DNA barcodes of marine...

journal_title：Molecular ecology resources

authors： Naro-Maciel E,Reid B,Fitzsimmons NN,LE M,Desalle R,Amato G

更新日期：2010-03-01 00:00:00

abstract：:Photosynthetic eukaryotes have a critical role as the main producers in most ecosystems of the biosphere. The ongoing environmental metabarcoding revolution opens the perspective for holistic ecosystems biological studies of these organisms, in particular the unicellular microalgae that often lack distinctive morpholo...

journal_title：Molecular ecology resources

authors： Decelle J,Romac S,Stern RF,Bendif el M,Zingone A,Audic S,Guiry MD,Guillou L,Tessier D,Le Gall F,Gourvil P,Dos Santos AL,Probert I,Vaulot D,de Vargas C,Christen R

更新日期：2015-11-01 00:00:00

abstract：:This article documents the public availability of approximately 15 000 polymorphic SNP loci for the bighorn sheep Ovis canadensis. ...

journal_title：Molecular ecology resources

authors： Genomic Resources Development Consortium.,Coltman DW,Hogg JT,Miller JM

更新日期：2013-09-01 00:00:00

abstract：:We describe primers and polymerase chain reaction conditions to amplify 22 microsatellite loci from the Barrow's goldeneye (Bucephala islandica). The primers were tested on 27 individuals from a population breeding in British Columbia, Canada. The developed primer pairs yielded an average of 6.11 alleles per locus (ra...

journal_title：Molecular ecology resources

authors： Jaari S,Jaatinen K,Merilä J

更新日期：2009-05-01 00:00:00

abstract：:We describe the cloning and characterization of eight novel tetranucleotide microsatellite loci in the ornate chorus frog (Pseudacris ornata). We also screened 26 loci from GenBank that were isolated from other Pseudacris species and obtained consistent product from five of these dinucleotide loci. All loci are polymo...

journal_title：Molecular ecology resources

authors： Degner JF,Hether TD,Hoffman EA

更新日期：2009-03-01 00:00:00

abstract：:We introduce phylodyn, an r package for phylodynamic analysis based on gene genealogies. The package's main functionality is Bayesian nonparametric estimation of effective population size fluctuations over time. Our implementation includes several Markov chain Monte Carlo-based methods and an integrated nested Laplace...

journal_title：Molecular ecology resources

authors： Karcher MD,Palacios JA,Lan S,Minin VN

更新日期：2017-01-01 00:00:00

abstract：:Estimation of demographic history from nucleotide sequences represents an important component of many studies in molecular ecology. For example, knowledge of a population's history can allow us to test hypotheses about the impact of climatic and anthropogenic factors. In the past, demographic analysis was typically li...

journal_title：Molecular ecology resources

authors： Ho SY,Shapiro B

更新日期：2011-05-01 00:00:00

abstract：:We present 10 microsatellite markers for the buttonwood mangrove, Conocarpus erectus, a wide-range mangrove associate species. Polymorphism was assessed among individuals from six different populations along the Pacific Coast of Mexico and Costa Rica, as well as in two individuals from the Yucatan Peninsula in the Atl...

journal_title：Molecular ecology resources

authors： Nettel A,Dodd RS,Cid-Becerra JA,DE LA Rosa-Velez J

更新日期：2008-07-01 00:00:00

abstract：:Global climate change (GCC) may be causing distribution range shifts in many organisms worldwide. Multiple efforts are currently focused on the development of models to better predict distribution range shifts due to GCC. We addressed this issue by including intraspecific genetic structure and spatial autocorrelation ...

journal_title：Molecular ecology resources

authors： Martínez-Minaya J,Conesa D,Fortin MJ,Alonso-Blanco C,Picó FX,Marcer A

更新日期：2019-07-01 00:00:00

abstract：:A variety of challenges arise when monitoring wildlife populations for disease. Sampling tissues can be invasive to hosts, and obtaining sufficient sample sizes can be expensive and time-consuming, particularly for rare species and when pathogen prevalence is low. Environmental DNA (eDNA)-based detection of pathogens ...

journal_title：Molecular ecology resources

authors： Hall EM,Crespi EJ,Goldberg CS,Brunner JL

更新日期：2016-03-01 00:00:00

abstract：:We report the characterization of eight microsatellite markers in the big-headed ant Pheidole megacephala, a pest ant registered in the list of '100 of the world's worst invasive alien species'. An enrichment protocol was used to isolate microsatellite loci, and polymorphism was explored with 36 individuals collected ...

journal_title：Molecular ecology resources

authors： Fournier D,Dubois D,Aron S

更新日期：2008-07-01 00:00:00

abstract：:Most hosts are concurrently or sequentially infected with multiple parasites; thus, fully understanding interactions between individual parasite species and their hosts depends on accurate characterization of the parasite community. For parasitic nematodes, noninvasive methods for obtaining quantitative, species-speci...

journal_title：Molecular ecology resources

authors： Budischak SA,Hoberg EP,Abrams A,Jolles AE,Ezenwa VO

更新日期：2015-09-01 00:00:00

abstract：:Plastid sequencing is an essential tool in the study of plant evolution. This high-copy organelle is one of the most technically accessible regions of the genome, and its sequence conservation makes it a valuable region for comparative genome evolution, phylogenetic analysis and population studies. Here, we discuss re...

journal_title：Molecular ecology resources

authors： Twyford AD,Ness RW

更新日期：2017-09-01 00:00:00

abstract：:DNA barcoding is a molecular method that rapidly identifies an individual to a known taxon or its closest relative based on a 650-bp fragment of the cytochrome c oxidase subunit I (COI). In this study, DNA barcodes of members of the family Accipitridae, including Haliastur indus (brahminy kite), Haliaeetus leucogaster...

journal_title：Molecular ecology resources

authors： Ong PS,Luczon AU,Quilang JP,Sumaya AM,Ibañez JC,Salvador DJ,Fontanilla IK

更新日期：2011-03-01 00:00:00

abstract：:Exon markers have a long history of use in phylogenetics of ray-finned fishes, the most diverse clade of vertebrates with more than 35,000 species. As the number of published genomes increases, it has become easier to test exons and other genetic markers for signals of ancient duplication events and filter out paralog...

journal_title：Molecular ecology resources

authors： Hughes LC,Ortí G,Saad H,Li C,White WT,Baldwin CC,Crandall KA,Arcila D,Betancur-R R

更新日期：2020-10-21 00:00:00

abstract：:Long columns of migrating larval sciarid armyworms were discovered in central and northern Japan, specifically Kanagawa, Gunma, Miyagi and Akita prefectures, as well as Hokkaido. This is the first examination of armyworms in East Asia. In Europe, armyworms have been identified as Sciara militaris, belonging to the fam...

journal_title：Molecular ecology resources

authors： Sutou M,Kato T,Ito M

更新日期：2011-11-01 00:00:00

abstract：:The pygmy grasshopper Tetrix japonica is a common insect distributed throughout the world, and it has the potential for use in studies of body colour polymorphism, genomics and the biology of Tetrigoidea (Insecta: Orthoptera). However, limited biological information is available for this insect. Here, we conducted a d...

journal_title：Molecular ecology resources

authors： Qiu Z,Liu F,Lu H,Huang Y

更新日期：2017-05-01 00:00:00

abstract：:DNA extraction from minute hymenopterans and their larvae is difficult and challenging because of their small size indicating a low amount of starting material. Hence, 11 DNA extraction methods were compared to determine their efficacy in isolating DNA. Success of each method was scored on a 2% agarose gel after PCR o...

journal_title：Molecular ecology resources

authors： Dittrich-Schröder G,Wingfield MJ,Klein H,Slippers B

更新日期：2012-01-01 00:00:00

abstract：:With striking morphological diversity and adaptability, Chamaedorea palms constitute an ecologically and economically important understorey component of Neotropical forests. Nine loci developed for Chamaedorea elegans evaluated in three Veracruz populations resulted in a large number of alleles (8-18), and high expect...

journal_title：Molecular ecology resources

authors： Cibrián-Jaramillo A,Hahn WJ,Desalle R

更新日期：2008-03-01 00:00:00

abstract：:DNA metabarcoding was utilized for a large-scale, multiyear assessment of biodiversity in Malaise trap collections from the Bavarian Forest National Park (Germany, Bavaria). Principal component analysis of read count-based biodiversities revealed clustering in concordance with whether collection sites were located ins...

journal_title：Molecular ecology resources

authors： Hardulak LA,Morinière J,Hausmann A,Hendrich L,Schmidt S,Doczkal D,Müller J,Hebert PDN,Haszprunar G

更新日期：2020-11-01 00:00:00

abstract：:We report an accurate multiplex reverse transcription quantitative polymerase chain reaction (RT-qPCR) assay, capable of reproducing gene expression profiles from 16 target genes [12 genes of interest (GOIs) and four reference genes (RGs)] in Acropora millepora, a common reef-building model coral species. The 12 GOIs ...

journal_title：Molecular ecology resources

authors： Souter P,Bay LK,Andreakis N,Császár N,Seneca FO,van Oppen MJ

更新日期：2011-03-01 00:00:00

abstract：:The interbreeding of individuals coming from genetically differentiated but incompletely isolated populations can lead to the formation of admixed populations, having important implications in ecology and evolution. In this simulation study, we evaluate how individual admixture proportions estimated by the software st...

journal_title：Molecular ecology resources

authors： Toyama KS,Crochet PA,Leblois R

更新日期：2020-11-01 00:00:00

abstract：:The ability to detect the identity of a sample obtained from its environment is a cornerstone of molecular ecological research. Thanks to the falling price of shotgun sequencing, genome skimming, the acquisition of short reads spread across the genome at low coverage, is emerging as an alternative to traditional barco...

journal_title：Molecular ecology resources

authors： Rachtman E,Balaban M,Bafna V,Mirarab S

更新日期：2020-05-01 00:00:00

abstract：:In this study, we reported the isolation and analysis of new polymorphic microsatellites in mungbean (Vigna radiata (L.) Wilczek). Twelve out of 210 primer pairs screened in 30 mungbean accessions gave polymorphism. The polymorphic markers detected two to three alleles per locus with an average of 2.08. Observed heter...

journal_title：Molecular ecology resources

authors： Somta P,Musch W,Kongsamai B,Chanprame S,Nakasathien S,Toojinda T,Sorajjapinun W,Seehalak W,Tragoonrung S,Srinives P

更新日期：2008-09-01 00:00:00

abstract：:To characterize microbiomes and other ecological assemblages, ecologists routinely sequence and compare loci that differ among focal taxa. Counts of these sequences convey information regarding the occurrence and relative abundances of taxa, but provide no direct measure of their absolute abundances, due to the techni...

journal_title：Molecular ecology resources

authors： Harrison JG,John Calder W,Shuman B,Alex Buerkle C

更新日期：2021-01-01 00:00:00

abstract：:Twenty-four simple sequence repeat markers were developed for Phakopsora pachyrhizi, a fungal pathogen of soybean (Glycine max) and other legumes. All 24 of the loci were evaluated on 28 isolates of P. pachyrhizi. Twenty-one loci were polymorphic, with allelic diversity ranging from two to eight alleles, and null alle...

journal_title：Molecular ecology resources

authors： Anderson SJ,Stone CL,Posada-Buitrago ML,Boore JL,Neelam BA,Stephens RM,Luster DG,Frederick RD,Pedley KF

更新日期：2008-11-01 00:00:00

abstract：:Microsatellite markers have played a major role in ecological, evolutionary and conservation research during the past 20 years. However, technical constrains related to the use of capillary electrophoresis and a recent technological revolution that has impacted other marker types have brought to question the continued...

journal_title：Molecular ecology resources

authors： De Barba M,Miquel C,Lobréaux S,Quenette PY,Swenson JE,Taberlet P

更新日期：2017-05-01 00:00:00

abstract：:We have developed eight high-quality microsatellite DNA loci for the saltmarsh sharp-tailed sparrow and one additional locus with evidence of null alleles. In a sample of 250-350 individuals, the average number of alleles per locus was 14.7 and average observed heterozygosity was 0.80. These loci were tested in three ...

journal_title：Molecular ecology resources

authors： Hill CE,Tomko S,Hagen C,Schable NA,Glenn TC

更新日期：2008-01-01 00:00:00