Ten new microsatellite markers for the buttonwood mangrove (Conocarpus erectus L., Combretaceae).

abstract：:Microsatellites are valuable markers for the analysis of genetic diversity, linkage mapping or genotyping. The limited availability of microsatellites for the genus Potentilla (Rosaceae) stipulated the isolation of markers from a representative (Potentilla pusilla Host) of the Potentilla core group that constitutes th...

journal_title：Molecular ecology resources

authors： Dobeš Ch,Scheffknecht S

更新日期：2012-07-01 00:00:00

abstract：:We developed four multiplex panels comprising 19 microsatellite loci and tested their amplification in 21 rodent species important for agricultural and conservation management (Microtus, Arvicola, Chionomys). On average, 17.6 loci amplified per species. Number of alleles ranged from 1 to 19 per locus. We report an add...

journal_title：Molecular ecology resources

authors： Rudá M,Ziak D,Gauffre B,Zima J,Martínková N

更新日期：2009-05-01 00:00:00

abstract：:This article documents the addition of 153 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Brassica oleracea, Brycon amazonicus, Dimorphandra wilsonii, Eupallasella percnurus, Helleborus foetidus, Ipomoea purpurea, Phrynops geoffroanus, Prochilodus...

journal_title：Molecular ecology resources

authors： Molecular Ecology Resources Primer Development Consortium.,Aksoy S,Almeida-Val VM,Azevedo VC,Baucom R,Bazaga P,Beheregaray LB,Bennetzen JL,Brassaloti RA,Burgess TI,Caccone A,Chang SM,Ciampi AY,Ciancaleoni S,Clímaco GT,Cloue

更新日期：2013-03-01 00:00:00

abstract：:A variety of research projects focus on genetic variation among and within maternal lineages as encompassed by mitochondrial DNA (mtDNA). While mtDNA often differs substantially between species, large differences may also be found within species. The evaluation of such divergent lineages, for example in intraspecific ...

journal_title：Molecular ecology resources

authors： Lindell J,Murphy RW

更新日期：2008-01-01 00:00:00

abstract：:Twelve polymorphic microsatellite loci were isolated for the bonefish, Albula vulpes using a polymerase chain reaction-based procedure. The number of alleles ranged from two to 23 (mean = 8.8) in 37 specimens from south Florida. Observed and expected heterozygosities ranged from 0.07 to 0.77 (mean = 0.42) and from 0.0...

journal_title：Molecular ecology resources

authors： Seyoum S,Wallace EM,Tringali MD

更新日期：2008-03-01 00:00:00

abstract：:We developed 18 polymorphic microsatellite markers for Coregonus lavaretus from genomic libraries enriched for (GACA)(n) and (GATA)(n) repeat sequences. Emphasis was placed on developing highly polymorphic, perfect repeats. These loci were screened in 69 individuals from two alpine populations in Austria. Allelic vari...

journal_title：Molecular ecology resources

authors： Winkler KA,Weiss S

更新日期：2008-09-01 00:00:00

abstract：:The interbreeding of individuals coming from genetically differentiated but incompletely isolated populations can lead to the formation of admixed populations, having important implications in ecology and evolution. In this simulation study, we evaluate how individual admixture proportions estimated by the software st...

journal_title：Molecular ecology resources

authors： Toyama KS,Crochet PA,Leblois R

更新日期：2020-11-01 00:00:00

abstract：:Archived specimens are highly valuable sources of DNA for retrospective genetic/genomic analysis. However, often limited effort has been made to evaluate and optimize extraction methods, which may be crucial for downstream applications. Here, we assessed and optimized the usefulness of abundant archived skeletal mater...

journal_title：Molecular ecology resources

authors： Nielsen EE,Morgan JAT,Maher SL,Edson J,Gauthier M,Pepperell J,Holmes BJ,Bennett MB,Ovenden JR

更新日期：2017-05-01 00:00:00

abstract：:Anolis lizards are important models in studies of ecology and evolution. Here we describe 13 polymorphic microsatellites for use in population screening in the St Lucia anole, Anolis luciae, that can be used as a natural replicate to Anolis roquet on Martinique to study processes involved in population differentiation...

journal_title：Molecular ecology resources

authors： Johansson H,Surget-Groba Y,Gow JL,Thorpe RS

更新日期：2008-11-01 00:00:00

abstract：:A significant portion of plant species are polyploids, with ploidy levels sometimes varying among individuals and/or populations. Current techniques to determine the individual ploidy, e.g., flow cytometry, chromosome counting or genotyping-by-sequencing, are often cumbersome. Based on the genotypic probabilities for ...

journal_title：Molecular ecology resources

authors： Huang K,Dunn DW,Li Z,Zhang P,Dai Y,Li B

更新日期：2019-09-01 00:00:00

abstract：:We isolated 18 microsatellites from Sycoscapter australis, a nonpollinating fig wasp that develops in figs of Ficus macrophylla, and assessed their variability in 20 wasps. We further optimized nine of these loci for use in three other Sycoscapter species that develop in Ficus rubiginosa figs and assessed their variab...

journal_title：Molecular ecology resources

authors： Bouteiller-Reuter C,Hale ML,Cook JM

更新日期：2009-05-01 00:00:00

abstract：:The pygmy grasshopper Tetrix japonica is a common insect distributed throughout the world, and it has the potential for use in studies of body colour polymorphism, genomics and the biology of Tetrigoidea (Insecta: Orthoptera). However, limited biological information is available for this insect. Here, we conducted a d...

journal_title：Molecular ecology resources

authors： Qiu Z,Liu F,Lu H,Huang Y

更新日期：2017-05-01 00:00:00

abstract：:We report results of cross-species amplification in Dactylis glomerata and Lolium perenne of 12 simple sequence repeats (SSRs) isolated from Lolium multiflorum×Festuca glaucescens, 42 SSRs from Festuca arundinacea and two sequence tagged sites from Oryza sativa. We compared the transferability and diversity between D....

journal_title：Molecular ecology resources

authors： Litrico I,Bech N,Flajoulot S,Cadier D,Talon C,Gibelin C,Barre P

更新日期：2009-01-01 00:00:00

abstract：:Luffa spp. (sponge gourd or ridge gourd) is an economically important vegetable crop widely cultivated in China, India and Southeast Asia. Here, we employed PacBio long-read single-molecule real-time (SMRT) sequencing to perform de novo genome assemblies of two commonly cultivated Luffa species, L. acutangula and L. c...

journal_title：Molecular ecology resources

authors： Pootakham W,Sonthirod C,Naktang C,Nawae W,Yoocha T,Kongkachana W,Sangsrakru D,Jomchai N,U-Thoomporn S,Sheedy JR,Buaboocha J,Mekiyanon S,Tangphatsornruang S

更新日期：2021-01-01 00:00:00

abstract：:DNA metabarcoding was utilized for a large-scale, multiyear assessment of biodiversity in Malaise trap collections from the Bavarian Forest National Park (Germany, Bavaria). Principal component analysis of read count-based biodiversities revealed clustering in concordance with whether collection sites were located ins...

journal_title：Molecular ecology resources

authors： Hardulak LA,Morinière J,Hausmann A,Hendrich L,Schmidt S,Doczkal D,Müller J,Hebert PDN,Haszprunar G

更新日期：2020-11-01 00:00:00

abstract：:GONe is a user-friendly, Windows-based program for estimating effective size (N(e) ) in populations with overlapping generations. It uses the Jorde-Ryman modification to the temporal method to account for age structure in populations. This method requires estimates of age-specific survival and birth rate and allele fr...

journal_title：Molecular ecology resources

authors： Coombs JA,Letcher BH,Nislow KH

更新日期：2012-01-01 00:00:00

abstract：:Local adaptation is often studied via (i) multiple common garden experiments comparing performance of genotypes in different environments and (ii) sequencing genotypes from multiple locations and characterizing geographic patterns in allele frequency. Both approaches aim to characterize the same pattern (local adaptat...

journal_title：Molecular ecology resources

authors： Lasky JR,Forester BR,Reimherr M

更新日期：2018-01-01 00:00:00

abstract：:In this study, 49 primers were designed from sequences containing di-, tri-, tetra-, penta- and hexanucleotide motifs with a minimum of four repeats and presence of motif size polymorphisms (insertion/deletion) from cassava (Manihot esculenta Crantz) expressed sequence tags deposited in public sequence database. Each ...

journal_title：Molecular ecology resources

authors： Tangphatsornruang S,Sraphet S,Singh R,Okogbenin E,Fregene M,Triwitayakorn K

更新日期：2008-05-01 00:00:00

abstract：:Restriction fragment length polymorphism tools is an R application which supports a complete workflow of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), dealing with the problems which accompany analysis when PCR-RFLP is used in diversity studies. Large numbers of different RFLP samples ...

journal_title：Molecular ecology resources

authors： Flessa F,Kehl A,Kohl M

更新日期：2013-07-01 00:00:00

abstract：:We developed nine new nuclear dinucleotide microsatellite loci for Castor canadensis. All loci were polymorphic, except for one. The number of alleles ranged from two to four and from five to 12 in populations from Arizona and Wisconsin, respectively. Average heterozygosity ranged from 0.13 to 0.86 per locus. Since cr...

journal_title：Molecular ecology resources

authors： Pelz-Serrano K,Munguia-Vega A,Piaggio AJ,Neubaum M,Munclinger P,Pártl A,VAN Riper Iii C,Culver M

更新日期：2009-03-01 00:00:00

abstract：:Species identification based on short sequences of DNA markers, that is, DNA barcoding, has emerged as an integral part of modern taxonomy. However, software for the analysis of large and multilocus barcoding data sets is scarce. The Basic Local Alignment Search Tool (BLAST) is currently the fastest tool capable of ha...

journal_title：Molecular ecology resources

authors： Fan L,Hui JH,Yu ZG,Chu KH

更新日期：2014-07-01 00:00:00

abstract：:Streamlining the development and genotyping of microsatellites in species for which no genetic information is available represents an important technical challenge to overcome in order to enable mainstream application of state-of-the-art population genetic analysis techniques in nonmodel organisms. Using the example o...

journal_title：Molecular ecology resources

authors： Lepais O,Bacles CF

更新日期：2011-07-01 00:00:00

abstract：:DNA barcodes are useful tools to identify and discover new species in a wide range of taxa. Here, we report the first barcode study of monogonont rotifers from fresh and brackish waters in Mexico, and discuss the taxonomic implications of this work. We used DNA barcodes based on the sequence of cytochrome oxidase I to...

journal_title：Molecular ecology resources

authors： García-Morales AE,Elías-Gutiérrez M

更新日期：2013-11-01 00:00:00

abstract：:Exon markers have a long history of use in phylogenetics of ray-finned fishes, the most diverse clade of vertebrates with more than 35,000 species. As the number of published genomes increases, it has become easier to test exons and other genetic markers for signals of ancient duplication events and filter out paralog...

journal_title：Molecular ecology resources

authors： Hughes LC,Ortí G,Saad H,Li C,White WT,Baldwin CC,Crandall KA,Arcila D,Betancur-R R

更新日期：2020-10-21 00:00:00

abstract：:The European flounder (Platichthys flesus L.) is used in ecotoxicological studies to provide detailed information on the effects of pollution on individual fish. Data on population and evolutionary level effects are, however, limited. Here, the isolation and characterization of 28 novel species specific microsatellite...

journal_title：Molecular ecology resources

authors： Tysklind N,Neuparth T,Ashcroft GR,Taylor MI,Lyons BP,McCarthy ID,Carvalho GR

更新日期：2009-05-01 00:00:00

abstract：:We describe a convenient, cost-effective and flexible medium-throughput single nucleotide polymorphism (SNP) genotyping method, Multiplex SNP-SCALE, which enables the simultaneous amplification by polymerase chain reaction (PCR) of up to 25 (or potentially more) loci followed by electrophoresis in an automated DNA seq...

journal_title：Molecular ecology resources

authors： Kenta T,Gratten J,Haigh NS,Hinten GN,Slate J,Butlin RK,Burke T

更新日期：2008-11-01 00:00:00

abstract：:We tested for cross-species amplification of microsatellite loci located throughout the domestic sheep (Ovis aries) genome in two north American mountain ungulates (bighorn sheep, Ovis canadensis, and mountain goats, Oreamnos americanus). We identified 247 new polymorphic markers in bighorn sheep (≥ 3 alleles in one o...

journal_title：Molecular ecology resources

authors： Poissant J,Shafer AB,Davis CS,Mainguy J,Hogg JT,Côté SD,Coltman DW

更新日期：2009-07-01 00:00:00

abstract：:In noninvasive genetic sampling, when genotyping error rates are high and recapture rates are low, misidentification of individuals can lead to overestimation of population size. Thus, estimating genotyping errors is imperative. Nonetheless, conducting multiple polymerase chain reactions (PCRs) at multiple loci is tim...

journal_title：Molecular ecology resources

authors： Hansen H,Ben-David M,McDonald DB

更新日期：2008-03-01 00:00:00

abstract：:Thirty-eight microsatellite loci were developed for the bird pollinated, autohexaploid, Eremophila glabra ssp. glabra. A genomic library was screened with dinucleotide and trinucleotide sequence repeats. Polymorphism ranged from one to 21 alleles per locus. Twenty-four loci exhibited null alleles, based on patterns of...

journal_title：Molecular ecology resources

authors： Elliott CP

更新日期：2009-07-01 00:00:00

abstract：:Horseshoe crabs, represented by only four extant species, have existed for around 500 million years. However, their existence is now under threat because of anthropogenic activities. The availability of genomic resources for these species will be valuable in planning appropriate conservation measures. Whole-genome seq...

journal_title：Molecular ecology resources

authors： Shingate P,Ravi V,Prasad A,Tay BH,Venkatesh B

更新日期：2020-11-01 00:00:00