PERMANENT GENETIC RESOURCES: Development of microsatellite markers of the Mexican understorey palm Chamaedorea elegans, cross-species genotyping, and amplification in congeners.

abstract：:Archived specimens are highly valuable sources of DNA for retrospective genetic/genomic analysis. However, often limited effort has been made to evaluate and optimize extraction methods, which may be crucial for downstream applications. Here, we assessed and optimized the usefulness of abundant archived skeletal mater...

journal_title：Molecular ecology resources

authors： Nielsen EE,Morgan JAT,Maher SL,Edson J,Gauthier M,Pepperell J,Holmes BJ,Bennett MB,Ovenden JR

更新日期：2017-05-01 00:00:00

abstract：:High-throughput sequencing technologies are a proposed solution for accessing the molecular data in historical specimens. However, degraded DNA combined with the computational demands of short-read assemblies has posed significant laboratory and bioinformatics challenges for de novo genome assembly. Linked-read or "sy...

journal_title：Molecular ecology resources

authors： Colella JP,Tigano A,MacManes MD

更新日期：2020-07-01 00:00:00

abstract：:We report the characterization of 25 microsatellite loci isolated from the macaroni penguin (Eudyptes chrysolophus). Thirteen loci were arranged into four multiplex sets for future genetic studies of macaroni penguin populations. All 25 loci were tested separately in each of four other penguin species [Adélie penguin ...

journal_title：Molecular ecology resources

authors： Ahmed S,Hart T,Dawson DA,Horsburgh GJ,Trathan PN,Rogers AD

更新日期：2009-11-01 00:00:00

abstract：:Honeybee subspecies have been affected by human activities in Europe over the past few decades. One such example is the importation of nonlocal subspecies of bees which has had an adverse impact on the geographical repartition and subsequently on the genetic diversity of the black honeybee Apis mellifera mellifera. To...

journal_title：Molecular ecology resources

authors： Bertrand B,Alburaki M,Legout H,Moulin S,Mougel F,Garnery L

更新日期：2015-05-01 00:00:00

abstract：:Microbial fitness is easy to measure in the laboratory, but difficult to measure in the field. Laboratory fitness assays make use of controlled conditions and genetically modified organisms, neither of which are available in the field. Among other applications, fitness assays can help researchers detect adaptation to ...

journal_title：Molecular ecology resources

authors： Boynton PJ,Stelkens R,Kowallik V,Greig D

更新日期：2017-05-01 00:00:00

abstract：:New DNA sequencing technologies are allowing researchers to explore the genomes of the millions of natural history specimens collected prior to the molecular era. Yet, we know little about how well specific next-generation sequencing (NGS) techniques work with the degraded DNA typically extracted from museum specimens...

journal_title：Molecular ecology resources

authors： McCormack JE,Tsai WL,Faircloth BC

更新日期：2016-09-01 00:00:00

abstract：:With the advent of next generation sequencing technologies, transcriptome level sequence collections are arising as prominent resources for the discovery of gene-based molecular markers. In a previous study more than 15,000 simple sequence repeats (SSRs) in expressed sequence tag (EST) sequences resulting from 454 pyr...

journal_title：Molecular ecology resources

authors： Lesser MR,Parchman TL,Buerkle CA

更新日期：2012-05-01 00:00:00

abstract：:We combined restriction site associated DNA sequencing (RADseq) using a hypomethylation-sensitive enzyme and messenger RNA sequencing (mRNAseq) to develop molecular markers for the 16 gigabase genome of Cedrus atlantica, a conifer tree species. With each method, Illumina(®) reads from one individual were used to gener...

journal_title：Molecular ecology resources

authors： Karam MJ,Lefèvre F,Dagher-Kharrat MB,Pinosio S,Vendramin GG

更新日期：2015-05-01 00:00:00

abstract：:Frequently, the diversity of umbrella taxa is invoked to predict patterns of other, less well-known, life. However, the utility of this strategy has been questioned. We tested whether a phylogenetic diversity (PD) analysis of CO1 DNA barcodes could act as a proxy for standard methods of determining sampling efficiency...

journal_title：Molecular ecology resources

authors： Smith MA,Fernandez-Triana J,Roughley R,Hebert PD

更新日期：2009-05-01 00:00:00

abstract：:Thirty-four novel microsatellite markers developed for wood cricket (Nemobius sylvestris) were tested and optimized. Twenty-five microsatellite loci were polymorphic, exhibiting between two and nine alleles. Observed heterozygosities ranged from 0.038 to 0.925. The microsatellites were also tested in a species belongi...

journal_title：Molecular ecology resources

authors： Vanhala T,Cottrell J

更新日期：2008-11-01 00:00:00

abstract：:We isolated and characterized 52 novel microsatellite markers from Florida largemouth bass, Micropterus salmoides floridanus, for use in conservation, management and population genetic studies. Markers were assessed in M. s. floridanus from peninsular Florida (n = 30) and averaged eight alleles per locus with observed...

journal_title：Molecular ecology resources

authors： Lutz-Carrillo DJ,Hagen C,Dueck LA,Glenn TC

更新日期：2008-01-01 00:00:00

abstract：:As coral populations decline worldwide in the face of ongoing environmental change, documenting their distribution, diversity and conservation status is now more imperative than ever. Accurate delimitation and identification of species is a critical first step. This task, however, is not trivial as morphological varia...

journal_title：Molecular ecology resources

authors： Erickson KL,Pentico A,Quattrini AM,McFadden CS

更新日期：2021-01-01 00:00:00

abstract：:Female Drosophila melanogaster frequently mate with multiple males in nature as shown through parentage analysis. Although polyandry is well documented, we know little about the timing between mating events in wild Drosophila populations due to the challenge of following behaviours of individual females. In this study...

journal_title：Molecular ecology resources

authors： Giardina TJ,Clark AG,Fiumera AC

更新日期：2017-11-01 00:00:00

abstract：:Molecular markers have been used to identify the sex of sampled individuals for several decades, but the time-consuming development phase prevented their application in many systems. Recently, a growing number of papers have applied reduced-representation sequencing (RRS) protocols to the identification of sex-specifi...

journal_title：Molecular ecology resources

authors： Moore JS,Benestan L

更新日期：2018-03-01 00:00:00

abstract：:Chimpanzee populations are diminishing as a consequence of human activities, and as a result this species is now endangered. In the context of conservation programmes, genetic data can add vital information, for instance on the genetic diversity and structure of threatened populations. Single nucleotide polymorphisms ...

journal_title：Molecular ecology resources

authors： Andrés O,Rönn AC,Bonhomme M,Kellermann T,Crouau-Roy B,Doxiadis G,Verschoor EJ,Goossens B,Domingo-Roura X,Bruford MW,Bosch M,Syvänen AC

更新日期：2008-05-01 00:00:00

abstract：:The pygmy grasshopper Tetrix japonica is a common insect distributed throughout the world, and it has the potential for use in studies of body colour polymorphism, genomics and the biology of Tetrigoidea (Insecta: Orthoptera). However, limited biological information is available for this insect. Here, we conducted a d...

journal_title：Molecular ecology resources

authors： Qiu Z,Liu F,Lu H,Huang Y

更新日期：2017-05-01 00:00:00

abstract：:Consensus primers, based on exon sequences of the cyp73 gene family coding for cinnamate 4-hydroxylase (C4H) of the lignin biosynthesis pathway, were designed for the tetraploid willow species Salix alba and Salix fragilis. Diagnostic alleles at species level were observed among introns of three cyp73 genes and allowe...

journal_title：Molecular ecology resources

authors： Trung le Q,VAN Puyvelde K,Triest L

更新日期：2008-03-01 00:00:00

abstract：:We report the isolation and characterization of eight microsatellite loci from the scleractinian coral, Acropora nobilis. The microsatellite loci were obtained using compound SSR primers or an enrichment protocol. All the loci were polymorphic with four to eight alleles per locus and observed heterozygosities ranging ...

journal_title：Molecular ecology resources

authors： Isomura N,Hidaka M

更新日期：2008-05-01 00:00:00

abstract：:This article documents the public availability of (i) microbiomes in diet and gut of larvae from the dipteran Dilophus febrilis using massive parallel sequencing, (ii) SNP and SSR discovery and characterization in the transcriptome of the Atlantic mackerel (Scomber scombrus, L) and (iii) assembled transcriptome for an...

journal_title：Molecular ecology resources

authors： Genomic Resources Development Consortium.,Álvarez P,Arthofer W,Coelho MM,Conklin D,Estonba A,Grosso AR,Helyar SJ,Langa J,Machado MP,Montes I,Pinho J,Rief A,Schartl M,Schlick-Steiner BC,Seeber J,Steiner FM,Vilas C

更新日期：2015-11-01 00:00:00

abstract：:Betel nut (Areca nut, Areca catechu L.) is a conspicuous and important cultivated plant of tropical and subtropical habitats throughout Southeast Asia and Oceania. As a significant cultural and social offering, the migration of betel nut associated with human dispersal is an important issue in ethnobotany and anthropo...

journal_title：Molecular ecology resources

authors： Hu CH,Huang CC,Hung KH,Hsu TW,Chiang TY

更新日期：2009-03-01 00:00:00

abstract：:The almendro (Dipteryx panamensis, Fabaceae) is a tetraploid tree native to the Atlantic lowland rainforests of Central America. We present nine microsatellite primer pairs amplified in three multiplexed reactions for 549 individuals from four sites in Costa Rica. All loci were polymorphic, ranging from three to 13 al...

journal_title：Molecular ecology resources

authors： Hanson TR,Brunsfeld SJ,Finegan B,Waits LP

更新日期：2008-03-01 00:00:00

abstract：:Sex-determining modes remain unknown in numerous species, notably in fishes, in which a variety of modalities have been reported. Additionally, noninvasive individual sexing is problematic for species without external sex attributes or for early life stages, requiring cytogenetic or molecular analyses when sex chromos...

journal_title：Molecular ecology resources

authors： Trenkel VM,Boudry P,Verrez-Bagnis V,Lorance P

更新日期：2020-11-01 00:00:00

abstract：:Restriction fragment length polymorphism tools is an R application which supports a complete workflow of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), dealing with the problems which accompany analysis when PCR-RFLP is used in diversity studies. Large numbers of different RFLP samples ...

journal_title：Molecular ecology resources

authors： Flessa F,Kehl A,Kohl M

更新日期：2013-07-01 00:00:00

abstract：:Entomopathogenic fungi in the genus Metarhizium are useful for biological control programmes against economically important arthropod pests worldwide. However, understanding the true diversity and ecology of these organisms is hampered by convergent morphologies between species. The application of molecular techniques...

journal_title：Molecular ecology resources

authors： Kepler RM,Rehner SA

更新日期：2013-03-01 00:00:00

abstract：:A novel set of microsatellite markers for mungbean [Vigna radiata (L.) Wilczek] was developed from the public sequence database. Seventy-eight primers were designed and evaluated for polymorphism among 22 cultivated accessions. Eight polymorphic loci detected two to three alleles per locus with an average of 2.25. The...

journal_title：Molecular ecology resources

authors： Seehalak W,Somta P,Sommanas W,Srinives P

更新日期：2009-05-01 00:00:00

abstract：:Oestrogenic wastewater treatment works (WwTW) effluents discharged into UK rivers have been shown to affect sexual development, including inducing intersex, in wild roach (Rutilus rutilus). This can result in a reduced breeding capability with potential population level impacts. In the absence of a sex probe for roach...

journal_title：Molecular ecology resources

authors： Lange A,Paris JR,Gharbi K,Cézard T,Miyagawa S,Iguchi T,Studholme DJ,Tyler CR

更新日期：2020-07-01 00:00:00

abstract：:To enable the accurate sexing of individuals of introduced populations of the small Indian mongoose, Herpestes auropunctatus, we designed a primer set for the amplification of the sex-specific fragments EIF2S3Y and EIF2S3X. Using this primer set, the expected amplification products were obtained for all samples of gen...

journal_title：Molecular ecology resources

authors： Murata C,Ogura G,Kuroiwa A

更新日期：2011-03-01 00:00:00

abstract：:GONe is a user-friendly, Windows-based program for estimating effective size (N(e) ) in populations with overlapping generations. It uses the Jorde-Ryman modification to the temporal method to account for age structure in populations. This method requires estimates of age-specific survival and birth rate and allele fr...

journal_title：Molecular ecology resources

authors： Coombs JA,Letcher BH,Nislow KH

更新日期：2012-01-01 00:00:00

abstract：:Despite the contribution of DNA barcoding towards understanding the biodiversity and distribution of species, the success of COI amplification has been quite variable when it comes to freshwater zooplankton (Elías-Gutiérrez & Valdez-Moreno 2008; Jeffery et al. 2011). Some genera of microcrustaceans seem to be more dif...

journal_title：Molecular ecology resources

authors： Prosser S,Martínez-Arce A,Elías-Gutiérrez M

更新日期：2013-11-01 00:00:00

abstract：:Thirty-eight microsatellite loci were developed for the bird pollinated, autohexaploid, Eremophila glabra ssp. glabra. A genomic library was screened with dinucleotide and trinucleotide sequence repeats. Polymorphism ranged from one to 21 alleles per locus. Twenty-four loci exhibited null alleles, based on patterns of...

journal_title：Molecular ecology resources

authors： Elliott CP

更新日期：2009-07-01 00:00:00