Abstract:
:Microsatellite loci were identified from Cornus kousa'National'. Primer pairs for 86 loci were developed and of these, eight were optimized and screened using genomic DNA from 22 kousa cultivars. All optimized loci were polymorphic and the number of alleles per locus ranged from three to 17. Observed heterozygosity ranged from 0 to 0.3 and expected heterozygosity ranged from 0.38 to 0.91. These microsatellites will be useful in population studies, and a breeding programme for cultivar development of Cornus species.
journal_name
Mol Ecol Resourjournal_title
Molecular ecology resourcesauthors
Wadl PA,Wang X,Scheffler BE,Rinehart TA,Trigiano RNdoi
10.1111/j.1755-0998.2007.02062.xsubject
Has Abstractpub_date
2008-07-01 00:00:00pages
780-2issue
4eissn
1755-098Xissn
1755-0998journal_volume
8pub_type
杂志文章abstract::Biodiversity analyses based on next-generation sequencing (NGS) platforms have developed by leaps and bounds in recent years. A PCR-free strategy, which can alleviate taxonomic bias, was considered as a promising approach to delivering reliable species compositions of targeted environments. The major impediment of suc...
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