Abstract:
:The protist Labyrinthula zosterae (Phylum Bigyra, sensu Tsui et al. 2009) has been identified as a causative agent of wasting disease in eelgrass (Zostera marina), of which the most intense outbreak led to the destruction of 90% of eelgrass beds in eastern North America and western Europe in the 1930s. Outbreaks still occur today, albeit at a smaller scale. Traditionally, L. zosterae has been quantified by measuring the necrotic area of Z. marina leaf tissue. This indirect method can however only lead to a very rough estimate of pathogen load. Here, we present a quantitative real-time polymerase chain reaction (qPCR) approach to directly detect and quantify L. zosterae in eelgrass tissue. Based on the internal transcribed spacer (ITS) sequences of rRNA genes, species-specific primers were designed. Using our qPCR, we were able to quantify accurately and specifically L. zosterae load both from culture and eelgrass leaves using material from Europe and North America. Our detection limit was less than one L. zosterae cell. Our results demonstrate the potential of this qPCR assay to provide rapid, accurate and sensitive molecular identification and quantification of L. zosterae. In view of declining seagrass populations worldwide, this method will provide a valuable tool for seagrass ecologists and conservation projects.
journal_name
Mol Ecol Resourjournal_title
Molecular ecology resourcesauthors
Bergmann N,Fricke B,Schmidt MC,Tams V,Beining K,Schwitte H,Boettcher AA,Martin DL,Bockelmann AC,Reusch TB,Rauch Gdoi
10.1111/j.1755-0998.2011.03051.xsubject
Has Abstractpub_date
2011-11-01 00:00:00pages
1076-81issue
6eissn
1755-098Xissn
1755-0998journal_volume
11pub_type
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