Abstract:
:Single nucleotide polymorphism (SNP) markers were identified and validated for two stingrays species, Potamotrygon motoro and Potamotrygon falkneri, using double digest restriction-site associated DNA (ddRAD) reads using 454-Roche technology. A total of 226 774 reads (65.5 Mb) were obtained (mean read length 289 ± 183 bp) detecting a total of 5399 contigs (mean contig length: 396 ± 91 bp). Mining this data set, a panel of 143 in silico SNPs was selected. Eighty-two of these SNPs were successfully validated and 61 were polymorphic: 14 in P. falkneri, 21 in P. motoro, 3 in both species and 26 fixed for alternative variants in both species, thus being useful for population analyses and hybrid detection.
journal_name
Mol Ecol Resourjournal_title
Molecular ecology resourcesauthors
Cruz VP,Vera M,Pardo BG,Taggart J,Martinez P,Oliveira C,Foresti Fdoi
10.1111/1755-0998.12564subject
Has Abstractpub_date
2017-05-01 00:00:00pages
550-556issue
3eissn
1755-098Xissn
1755-0998journal_volume
17pub_type
杂志文章abstract::Inventory of the caterpillars, their food plants and parasitoids began in 1978 for today's Area de Conservacion Guanacaste (ACG), in northwestern Costa Rica. This complex mosaic of 120 000 ha of conserved and regenerating dry, cloud and rain forest over 0-2000 m elevation contains at least 10 000 species of non-leaf-m...
journal_title:Molecular ecology resources
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abstract::Germplasm collections provide an extremely valuable resource for breeders and researchers. However, misclassification of accessions by species often hinders the effective use of these collections. We propose that use of high-throughput genotyping tools can provide a fast, efficient and cost-effective way of confirming...
journal_title:Molecular ecology resources
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journal_title:Molecular ecology resources
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journal_title:Molecular ecology resources
pub_type: 杂志文章
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journal_title:Molecular ecology resources
pub_type: 杂志文章
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pub_type: 杂志文章,评审
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journal_title:Molecular ecology resources
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journal_title:Molecular ecology resources
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journal_title:Molecular ecology resources
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journal_title:Molecular ecology resources
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