Identification and validation of single nucleotide polymorphisms as tools to detect hybridization and population structure in freshwater stingrays.

Abstract:

:Single nucleotide polymorphism (SNP) markers were identified and validated for two stingrays species, Potamotrygon motoro and Potamotrygon falkneri, using double digest restriction-site associated DNA (ddRAD) reads using 454-Roche technology. A total of 226 774 reads (65.5 Mb) were obtained (mean read length 289 ± 183 bp) detecting a total of 5399 contigs (mean contig length: 396 ± 91 bp). Mining this data set, a panel of 143 in silico SNPs was selected. Eighty-two of these SNPs were successfully validated and 61 were polymorphic: 14 in P. falkneri, 21 in P. motoro, 3 in both species and 26 fixed for alternative variants in both species, thus being useful for population analyses and hybrid detection.

journal_name

Mol Ecol Resour

authors

Cruz VP,Vera M,Pardo BG,Taggart J,Martinez P,Oliveira C,Foresti F

doi

10.1111/1755-0998.12564

subject

Has Abstract

pub_date

2017-05-01 00:00:00

pages

550-556

issue

3

eissn

1755-098X

issn

1755-0998

journal_volume

17

pub_type

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