Isolation of compound microsatellite loci in the herbaceous perennial Cirsium purpuratum (Maxim.) Matsum.

abstract：:Molecular genetic estimates of population effective size (Ne ) lose accuracy and precision when insufficient numbers of samples or loci are used. Ideally, researchers would like to forecast the necessary power when planning their project. neogen (genetic Ne for Overlapping Generations) enables estimates of precision a...

journal_title：Molecular ecology resources

authors： Blower DC,Riginos C,Ovenden JR

更新日期：2019-01-01 00:00:00

abstract：:The rice stem borer, Chilo suppressalis, is one of the most damaging insect pests to rice production worldwide. Although C. suppressalis has been the focus of numerous studies examining cold tolerance and diapause, plant-insect interactions, pesticide targets and resistance, and the development of RNAi-mediated pest m...

journal_title：Molecular ecology resources

authors： Ma W,Zhao X,Yin C,Jiang F,Du X,Chen T,Zhang Q,Qiu L,Xu H,Joe Hull J,Li G,Sung WK,Li F,Lin Y

更新日期：2020-01-01 00:00:00

abstract：:This study examined the interspecific amplification of nuclear microsatellite loci developed mainly for eucalypts in the subgenus Symphyomyrtus across five species within the second most speciose subgenus, subgenus Eucalyptus. A set of eight to 10 loci, depending on taxon, have been identified that are highly variable...

journal_title：Molecular ecology resources

authors： Nevill PG,Reed A,Bossinger G,Vaillancourt RE,Larcombe M,Ades PK

更新日期：2008-11-01 00:00:00

abstract：:Caribbean reef-building corals in the genus Acropora have been declining dramatically since the 1980s and are now listed as threatened. The study of their complex reproductive system (mixed asexual and sexual) and their population structure requires highly polymorphic nuclear genetic markers. Of eight previously devel...

journal_title：Molecular ecology resources

authors： Baums IB,Devlin-Durante MK,Brown L,Pinzón JH

更新日期：2009-07-01 00:00:00

abstract：:The European flounder (Platichthys flesus L.) is used in ecotoxicological studies to provide detailed information on the effects of pollution on individual fish. Data on population and evolutionary level effects are, however, limited. Here, the isolation and characterization of 28 novel species specific microsatellite...

journal_title：Molecular ecology resources

authors： Tysklind N,Neuparth T,Ashcroft GR,Taylor MI,Lyons BP,McCarthy ID,Carvalho GR

更新日期：2009-05-01 00:00:00

abstract：:Eight polymorphic microsatellite loci were isolated and characterized from a microsatellite DNA-enriched DNA library for the leopard coralgrouper (Plectropomus leopardus Lacepède, 1802), a popular food fish in the East Indies. These loci showed polymorphism information content ranging from 0.493 to 0.854, allele numbe...

journal_title：Molecular ecology resources

authors： Ding SX,Zeng HS,Wang Y,Pan Y,Shi XF

更新日期：2009-11-01 00:00:00

abstract：:Proportional and separate models able to apply different combination of substitution rate matrix (SRM) and among-site rate variation model (ASRVM) to each locus are frequently used in phylogenetic studies of multilocus data. A proportional model assumes that branch lengths are proportional among partitions and a separ...

journal_title：Molecular ecology resources

authors： Tanabe AS

更新日期：2011-09-01 00:00:00

abstract：:Karyotypic changes in chromosome number and structure are drivers in the divergent evolution of diverse plant species and lineages. This study aimed to reveal the origins of the unique karyotype (2n = 12) and phylogenetic relationships of the genus Megadenia (Brassicaceae). A high-quality chromosome-scale genome was a...

journal_title：Molecular ecology resources

authors： Yang W,Zhang L,Mandáková T,Huang L,Li T,Jiang J,Yang Y,Lysak MA,Liu J,Hu Q

更新日期：2020-11-05 00:00:00

abstract：:In order to facilitate the addition of a genetic component to conservation management plans for Georgia false indigo (Amorpha georgiana var. georgiana), a rare legume of the southeastern USA, 12 polymorphic microsatellite markers were developed. No gametic disequilibrium was detected among locus pairs, but observation...

journal_title：Molecular ecology resources

authors： Straub SC,Bogdanowicz SM,Doyle JJ

更新日期：2009-01-01 00:00:00

abstract：:DNA sourced from faeces is notoriously less reliable than that from tissue. Hence, understanding whether faecal pellet quality varies within faecal piles may be important for sample selection. We hypothesized that the order in which faecal pellets are deposited may influence microsatellite polymerase chain reaction (P...

journal_title：Molecular ecology resources

authors： Walker FM,Horsup A,Taylor AC

更新日期：2009-05-01 00:00:00

abstract：:Fifteen polymorphic markers were developed from a microsatellite-enriched library for the lizard Podarcis gaigeae. The loci were checked for variability in 68 individuals from a population on the island of Skyros, Greece. The number of alleles ranged from 3 to 23 per locus and expected heterozygosity from 0.29 and 0.9...

journal_title：Molecular ecology resources

authors： Wellenreuther M,Runemark A,Svensson EI,Hansson B

更新日期：2009-05-01 00:00:00

abstract：:Sex-determining modes remain unknown in numerous species, notably in fishes, in which a variety of modalities have been reported. Additionally, noninvasive individual sexing is problematic for species without external sex attributes or for early life stages, requiring cytogenetic or molecular analyses when sex chromos...

journal_title：Molecular ecology resources

authors： Trenkel VM,Boudry P,Verrez-Bagnis V,Lorance P

更新日期：2020-11-01 00:00:00

abstract：:The 'One Health' framework emphasizes the ecological relationships between soil, plant, animal and human health. Microbiomes play important roles in these relationships, as they modify the health and performance of the different compartments and influence the transfer of energy, matter and chemicals between them. Stan...

journal_title：Molecular ecology resources

authors： Wasimuddin,Schlaeppi K,Ronchi F,Leib SL,Erb M,Ramette A

更新日期：2020-11-01 00:00:00

abstract：:A multiplex haplotype-specific polymerase chain reaction (MHS-PCR) method was developed, which identified seven Clupeiform species living in the tropical Eastern Atlantic region: Sardinella aurita, Sardinella maderensis, Ethmalosa fimbriata, Sardina pilchardus, Engraulis encrasicholus, Pellonula leonensis and Ilisha a...

journal_title：Molecular ecology resources

authors： Durand JD,Diatta MA,Diop K,Trape S

更新日期：2010-05-01 00:00:00

abstract：:We introduce phylodyn, an r package for phylodynamic analysis based on gene genealogies. The package's main functionality is Bayesian nonparametric estimation of effective population size fluctuations over time. Our implementation includes several Markov chain Monte Carlo-based methods and an integrated nested Laplace...

journal_title：Molecular ecology resources

authors： Karcher MD,Palacios JA,Lan S,Minin VN

更新日期：2017-01-01 00:00:00

abstract：:sam is a Windows program designed to detect candidate loci for selection in whole-genome scans. It also gives valuable clues as regards the ecological factors at stake in the selection process. The method used is based on multiple univariate logistic regression models to test for association between allelic frequencie...

journal_title：Molecular ecology resources

authors： Joost S,Kalbermatten M,Bonin A

更新日期：2008-09-01 00:00:00

abstract：:In noninvasive genetic sampling, when genotyping error rates are high and recapture rates are low, misidentification of individuals can lead to overestimation of population size. Thus, estimating genotyping errors is imperative. Nonetheless, conducting multiple polymerase chain reactions (PCRs) at multiple loci is tim...

journal_title：Molecular ecology resources

authors： Hansen H,Ben-David M,McDonald DB

更新日期：2008-03-01 00:00:00

abstract：:We present the leaf and floral transcriptomes of two hybridizing bromeliad species that differ in their major pollinator systems. Here we identified candidate genes responsible for pollinator attraction and reproductive isolation in these two species. We searched for candidate genes involved in floral traits, such as ...

journal_title：Molecular ecology resources

authors： Palma-Silva C,Ferro M,Bacci M,Turchetto-Zolet AC

更新日期：2016-07-01 00:00:00

abstract：:Microsatellite genotyping of hair and faeces using standard polymerase chain reaction (PCR) resulted in low success rates and high error rates in a 2003-2004 pilot study using noninvasive genetic sampling for the brown bear (Ursus arctos) in the Italian Alps. Thus, we evaluated the performance of multiplex pre-amplifi...

journal_title：Molecular ecology resources

authors： DE Barba M,Waits LP

更新日期：2010-07-01 00:00:00

abstract：:Single nucleotide polymorphism (SNP) markers were identified and validated for two stingrays species, Potamotrygon motoro and Potamotrygon falkneri, using double digest restriction-site associated DNA (ddRAD) reads using 454-Roche technology. A total of 226 774 reads (65.5 Mb) were obtained (mean read length 289 ± 183...

journal_title：Molecular ecology resources

authors： Cruz VP,Vera M,Pardo BG,Taggart J,Martinez P,Oliveira C,Foresti F

更新日期：2017-05-01 00:00:00

abstract：:Reliable identification of Aphidiinae species (Braconidae) is a prerequisite for conducting studies on aphid-parasitoid interactions at the community level. However, morphological identification of Aphidiinae species remains problematic even for specialists and is almost impossible with larval stages. Here, we compare...

journal_title：Molecular ecology resources

authors： Derocles SA,LE Ralec A,Plantegenest M,Chaubet B,Cruaud C,Cruaud A,Rasplus JY

更新日期：2012-03-01 00:00:00

abstract：:This article documents the public availability of raw transcriptome sequence data and 63,020 SNPs for the polar bear (Ursus maritimus). ...

journal_title：Molecular ecology resources

authors： Genomic Resources Development Consortium.,Coltman DW,Davis CS,Lunn NJ,Malenfant RM,Richardson ES

更新日期：2014-01-01 00:00:00

abstract：:Ecological understanding of the role of consumer-resource interactions in natural food webs is limited by the difficulty of accurately and efficiently determining the complex variety of food types animals have eaten in the field. We developed a method based on DNA metabarcoding multiplexing and next-generation sequenc...

journal_title：Molecular ecology resources

authors： De Barba M,Miquel C,Boyer F,Mercier C,Rioux D,Coissac E,Taberlet P

更新日期：2014-03-01 00:00:00

abstract：:Our study evaluated in silico the potential of 14 mitochondrial genes encoding the subunits of the respiratory chain complexes, including cytochrome c oxidase I (CO1), as Basidiomycota DNA barcode. Fifteen complete and partial mitochondrial genomes were recovered and characterized in this study. Mitochondrial genes sh...

journal_title：Molecular ecology resources

authors： Vialle A,Feau N,Allaire M,Didukh M,Martin F,Moncalvo JM,Hamelin RC

更新日期：2009-05-01 00:00:00

abstract：:Molecular markers have been used to identify the sex of sampled individuals for several decades, but the time-consuming development phase prevented their application in many systems. Recently, a growing number of papers have applied reduced-representation sequencing (RRS) protocols to the identification of sex-specifi...

journal_title：Molecular ecology resources

authors： Moore JS,Benestan L

更新日期：2018-03-01 00:00:00

abstract：:In this study, 49 primers were designed from sequences containing di-, tri-, tetra-, penta- and hexanucleotide motifs with a minimum of four repeats and presence of motif size polymorphisms (insertion/deletion) from cassava (Manihot esculenta Crantz) expressed sequence tags deposited in public sequence database. Each ...

journal_title：Molecular ecology resources

authors： Tangphatsornruang S,Sraphet S,Singh R,Okogbenin E,Fregene M,Triwitayakorn K

更新日期：2008-05-01 00:00:00

abstract：:Restriction-site-associated DNA tag (RAD-tag) sequencing has become a popular approach to generate thousands of SNPs used to address diverse questions in population genomics. Comparatively, the suitability of RAD-tag genotyping to address evolutionary questions across divergent species has been the subject of only a f...

journal_title：Molecular ecology resources

authors： Viricel A,Pante E,Dabin W,Simon-Bouhet B

更新日期：2014-05-01 00:00:00

abstract：:We combined restriction site associated DNA sequencing (RADseq) using a hypomethylation-sensitive enzyme and messenger RNA sequencing (mRNAseq) to develop molecular markers for the 16 gigabase genome of Cedrus atlantica, a conifer tree species. With each method, Illumina(®) reads from one individual were used to gener...

journal_title：Molecular ecology resources

authors： Karam MJ,Lefèvre F,Dagher-Kharrat MB,Pinosio S,Vendramin GG

更新日期：2015-05-01 00:00:00

abstract：:Species identification based on short sequences of DNA markers, that is, DNA barcoding, has emerged as an integral part of modern taxonomy. However, software for the analysis of large and multilocus barcoding data sets is scarce. The Basic Local Alignment Search Tool (BLAST) is currently the fastest tool capable of ha...

journal_title：Molecular ecology resources

authors： Fan L,Hui JH,Yu ZG,Chu KH

更新日期：2014-07-01 00:00:00

abstract：:The rice leaffolder, Cnaphalocrocis medinalis Guenée (Crambidae, Lepidoptera), is an important agricultural pest that causes serious losses to rice production in rice-growing regions with high humidity and temperature. However, a lack of genomic resources limits in-depth understanding of its biological characteristics...

journal_title：Molecular ecology resources

authors： Zhao X,Xu H,He K,Shi Z,Chen X,Ye X,Mei Y,Yang Y,Li M,Gao L,Xu L,Xiao H,Liu Y,Lu Z,Li F

更新日期：2021-02-01 00:00:00