Abstract:
:Introgression can be an important evolutionary force but it can also lead to species extinction and as such is a crucial issue for species conservation. However, introgression is difficult to detect, morphologically as well as genetically. Hybridization with domestic cats (Felis silvestris catus) is a major concern for the conservation of European wildcats (Felis s. silvestris). The available morphologic and genetic markers for the two Felis subspecies are not sufficient to reliably detect hybrids beyond first generation. Here we present a single nucleotide polymorphism (SNP) based approach that allows the identification of introgressed individuals. Using high-throughput sequencing of reduced representation libraries we developed a diagnostic marker set containing 48 SNPs (Fst > 0.8) which allows the identification of wildcats, domestic cats, their hybrids and backcrosses. This allows assessing introgression rate in natural wildcat populations and is key for a better understanding of hybridization processes.
journal_name
Mol Ecol Resourjournal_title
Molecular ecology resourcesauthors
Nussberger B,Greminger MP,Grossen C,Keller LF,Wandeler Pdoi
10.1111/1755-0998.12075subject
Has Abstractpub_date
2013-05-01 00:00:00pages
447-60issue
3eissn
1755-098Xissn
1755-0998journal_volume
13pub_type
杂志文章abstract::Molecular markers have been used to identify the sex of sampled individuals for several decades, but the time-consuming development phase prevented their application in many systems. Recently, a growing number of papers have applied reduced-representation sequencing (RRS) protocols to the identification of sex-specifi...
journal_title:Molecular ecology resources
pub_type: 评论,新闻
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