Characterization of new microsatellite markers derived from sequence databases for the emu (Dromaius novaehollandiae).

Abstract:

:The emu (Dromaius novaehollandiae), a member of ratite family, is native to Australia and has been introduced to other countries worldwide. In this work, 10 polymorphic microsatellite loci were isolated and characterized for emu from public sequences. Polymorphism was surveyed in 22 individuals from two different populations kept in captivity. Between two and 11 alleles were found per locus, and the observed heterozygosity ranged from 0.05 to 0.85, in accordance with expectations. These markers will be useful as tools for detecting levels of genetic variation, reconstructing pedigrees (for quantitative genetic analysis) and identifying markers associated to fitness traits in emu populations.

journal_name

Mol Ecol Resour

authors

Yáñez JM,González R,Angulo J,Vidal R,Santos JL,Martínez V

doi

10.1111/j.1755-0998.2008.02315.x

subject

Has Abstract

pub_date

2008-11-01 00:00:00

pages

1442-4

issue

6

eissn

1755-098X

issn

1755-0998

journal_volume

8

pub_type

杂志文章
  • Molecular methods for arthropod bloodmeal identification and applications to ecological and vector-borne disease studies.

    abstract::DNA-based methods have greatly enhanced the sensitivity and specificity of hematophagous arthropod bloodmeal identification. A variety of methods have been applied to study the blood-feeding behaviour of mosquitoes, ticks, black flies and other blood-feeding arthropods as it relates to host-parasite interactions and p...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2008.02469.x

    authors: Kent RJ

    更新日期:2009-01-01 00:00:00

  • DNA barcoding of freshwater rotifera in Mexico: evidence of cryptic speciation in common rotifers.

    abstract::DNA barcodes are useful tools to identify and discover new species in a wide range of taxa. Here, we report the first barcode study of monogonont rotifers from fresh and brackish waters in Mexico, and discuss the taxonomic implications of this work. We used DNA barcodes based on the sequence of cytochrome oxidase I to...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12080

    authors: García-Morales AE,Elías-Gutiérrez M

    更新日期:2013-11-01 00:00:00

  • Isolation and characterization of microsatellites in the bird-pollinated, autohexaploid, Eremophila glabra ssp. glabra (R.Br. (Ostenf.)) (Myoporaceae), an Australian endemic plant.

    abstract::Thirty-eight microsatellite loci were developed for the bird pollinated, autohexaploid, Eremophila glabra ssp. glabra. A genomic library was screened with dinucleotide and trinucleotide sequence repeats. Polymorphism ranged from one to 21 alleles per locus. Twenty-four loci exhibited null alleles, based on patterns of...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2009.02618.x

    authors: Elliott CP

    更新日期:2009-07-01 00:00:00

  • Comparison of SNPs and microsatellites for fine-scale application of genetic stock identification of Chinook salmon in the Columbia River Basin.

    abstract::Genetic stock identification (GSI) is an important tool in fisheries management. Microsatellites (μSATs) have been the dominant genetic marker for GSI; however, increasing availability and numerous advantages of single-nucleotide polymorphism (SNP) markers make them an appealing alternative. We tested performance of 1...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2010.02958.x

    authors: Hess JE,Matala AP,Narum SR

    更新日期:2011-03-01 00:00:00

  • Polymorphic microsatellite markers in the Melon Fruit Fly, Bactrocera cucurbitae (Coquillett) (Diptera: Tephritidae).

    abstract::Bactrocera cucurbitae (Coquillett) is an important insect pest of melon fruit causing extensive losses of production in Asia and Pacific areas. Twelve polymorphic microsatellite loci were isolated from an enriched DNA library of this species and their amplification characteristics are described. Genetic parameters wer...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2009.02678.x

    authors: Wu Y,Li Z,Wu J

    更新日期:2009-09-01 00:00:00

  • Using next-generation sequencing for molecular reconstruction of past Arctic vegetation and climate.

    abstract::Palaeoenvironments and former climates are typically inferred from pollen and macrofossil records. This approach is time-consuming and suffers from low taxonomic resolution and biased taxon sampling. Here, we test an alternative DNA-based approach utilizing the P6 loop in the chloroplast trnL (UAA) intron; a short (13...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2010.02855.x

    authors: Sønstebø JH,Gielly L,Brysting AK,Elven R,Edwards M,Haile J,Willerslev E,Coissac E,Rioux D,Sannier J,Taberlet P,Brochmann C

    更新日期:2010-11-01 00:00:00

  • De novo transcriptomic analyses for non-model organisms: an evaluation of methods across a multi-species data set.

    abstract::High-throughput sequencing (HTS) is revolutionizing biological research by enabling scientists to quickly and cheaply query variation at a genomic scale. Despite the increasing ease of obtaining such data, using these data effectively still poses notable challenges, especially for those working with organisms without ...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12077

    authors: Singhal S

    更新日期:2013-05-01 00:00:00

  • Multiplex 16S rRNA haplotype-specific PCR, a rapid and convenient method for fish species identification: an application to West African Clupeiform larvae.

    abstract::A multiplex haplotype-specific polymerase chain reaction (MHS-PCR) method was developed, which identified seven Clupeiform species living in the tropical Eastern Atlantic region: Sardinella aurita, Sardinella maderensis, Ethmalosa fimbriata, Sardina pilchardus, Engraulis encrasicholus, Pellonula leonensis and Ilisha a...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2009.02776.x

    authors: Durand JD,Diatta MA,Diop K,Trape S

    更新日期:2010-05-01 00:00:00

  • Efficient screening of transgenic plant lines for ecological research.

    abstract::Plants stably transformed to manipulate the expression of genes mediating ecological performance have profoundly altered research in plant ecology. Agrobacterium-mediated transformation remains the most effective method of creating plants harbouring a limited number of transgene integrations of low complexity. For eco...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2011.03017.x

    authors: Gase K,Weinhold A,Bozorov T,Schuck S,Baldwin IT

    更新日期:2011-09-01 00:00:00

  • A quantitative real-time polymerase chain reaction assay for the seagrass pathogen Labyrinthula zosterae.

    abstract::The protist Labyrinthula zosterae (Phylum Bigyra, sensu Tsui et al. 2009) has been identified as a causative agent of wasting disease in eelgrass (Zostera marina), of which the most intense outbreak led to the destruction of 90% of eelgrass beds in eastern North America and western Europe in the 1930s. Outbreaks still...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2011.03051.x

    authors: Bergmann N,Fricke B,Schmidt MC,Tams V,Beining K,Schwitte H,Boettcher AA,Martin DL,Bockelmann AC,Reusch TB,Rauch G

    更新日期:2011-11-01 00:00:00

  • Chromosome-level genome assembly of the coastal horseshoe crab (Tachypleus gigas).

    abstract::Horseshoe crabs, represented by only four extant species, have existed for around 500 million years. However, their existence is now under threat because of anthropogenic activities. The availability of genomic resources for these species will be valuable in planning appropriate conservation measures. Whole-genome seq...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.13233

    authors: Shingate P,Ravi V,Prasad A,Tay BH,Venkatesh B

    更新日期:2020-11-01 00:00:00

  • Spatial analysis method (sam): a software tool combining molecular and environmental data to identify candidate loci for selection.

    abstract::sam is a Windows program designed to detect candidate loci for selection in whole-genome scans. It also gives valuable clues as regards the ecological factors at stake in the selection process. The method used is based on multiple univariate logistic regression models to test for association between allelic frequencie...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2008.02162.x

    authors: Joost S,Kalbermatten M,Bonin A

    更新日期:2008-09-01 00:00:00

  • Spider-specific probe set for ultraconserved elements offers new perspectives on the evolutionary history of spiders (Arachnida, Araneae).

    abstract::Phylogenomic methods have proven useful for resolving deep nodes and recalcitrant groups in the spider tree of life. Across arachnids, transcriptomic approaches may generate thousands of loci, and target-capture methods, using the previously designed arachnid-specific probe set, can target a maximum of about 1,000 loc...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.13099

    authors: Kulkarni S,Wood H,Lloyd M,Hormiga G

    更新日期:2020-01-01 00:00:00

  • Developing EPIC markers for chalcidoid Hymenoptera from EST and genomic data.

    abstract::Increasing numbers of phylogeographic studies make comparative inferences about the histories of co-distributed species. Although the aims of such studies are best achieved by jointly analysing sequences from multiple loci in a model-based framework, such data currently exist for few nonmodel systems. We used existing...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2010.02956.x

    authors: Lohse K,Sharanowski B,Blaxter M,Nicholls JA,Stone GN

    更新日期:2011-05-01 00:00:00

  • A factorial design experiment as a pilot study for noninvasive genetic sampling.

    abstract::Noninvasive genetic sampling has increasingly been used in ecological and conservation studies during the last decade. A major part of the noninvasive genetic literature is dedicated to the search for optimal protocols, by comparing different methods of collection, preservation and extraction of DNA from noninvasive m...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2012.03170.x

    authors: Renan S,Speyer E,Shahar N,Gueta T,Templeton AR,Bar-David S

    更新日期:2012-11-01 00:00:00

  • A primer set to determine sex in the small Indian mongoose, Herpestes auropunctatus.

    abstract::To enable the accurate sexing of individuals of introduced populations of the small Indian mongoose, Herpestes auropunctatus, we designed a primer set for the amplification of the sex-specific fragments EIF2S3Y and EIF2S3X. Using this primer set, the expected amplification products were obtained for all samples of gen...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2010.02931.x

    authors: Murata C,Ogura G,Kuroiwa A

    更新日期:2011-03-01 00:00:00

  • A linked-read approach to museomics: Higher quality de novo genome assemblies from degraded tissues.

    abstract::High-throughput sequencing technologies are a proposed solution for accessing the molecular data in historical specimens. However, degraded DNA combined with the computational demands of short-read assemblies has posed significant laboratory and bioinformatics challenges for de novo genome assembly. Linked-read or "sy...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.13155

    authors: Colella JP,Tigano A,MacManes MD

    更新日期:2020-07-01 00:00:00

  • Genomic resources notes accepted 1 April 2013-31 May 2013.

    abstract::This article documents the public availability of approximately 15 000 polymorphic SNP loci for the bighorn sheep Ovis canadensis. ...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12142

    authors: Genomic Resources Development Consortium.,Coltman DW,Hogg JT,Miller JM

    更新日期:2013-09-01 00:00:00

  • A newly developed genetic sex marker and its application to understanding chemically induced feminisation in roach (Rutilus rutilus).

    abstract::Oestrogenic wastewater treatment works (WwTW) effluents discharged into UK rivers have been shown to affect sexual development, including inducing intersex, in wild roach (Rutilus rutilus). This can result in a reduced breeding capability with potential population level impacts. In the absence of a sex probe for roach...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.13166

    authors: Lange A,Paris JR,Gharbi K,Cézard T,Miyagawa S,Iguchi T,Studholme DJ,Tyler CR

    更新日期:2020-07-01 00:00:00

  • The first transcriptome and genetic linkage map for Asian arowana.

    abstract::Asian arowana or dragonfish (Scleropages formosus) is an important fish species due to its unusual breeding biology and high economic value in the ornamental fish markets. In the present study, we aimed to (i) create the first transcriptome by Roche 454 pyrosequencing of Asian arowana brain and gonad samples; (ii) ide...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12212

    authors: Shen XY,Kwan HY,Thevasagayam NM,Prakki SR,Kuznetsova IS,Ngoh SY,Lim Z,Feng F,Chang A,Orbán L

    更新日期:2014-05-01 00:00:00

  • ednaoccupancy: An r package for multiscale occupancy modelling of environmental DNA data.

    abstract::In this article, we describe ednaoccupancy, an r package for fitting Bayesian, multiscale occupancy models. These models are appropriate for occupancy surveys that include three nested levels of sampling: primary sample units within a study area, secondary sample units collected from each primary unit and replicates o...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12735

    authors: Dorazio RM,Erickson RA

    更新日期:2018-03-01 00:00:00

  • Polymorphic microsatellite DNA markers for Daviesia triflora (Papilionaceae).

    abstract::We developed 11 polymorphic microsatellite DNA markers for an Australian native plant, Daviesia triflora. The number of alleles per locus in 40 individuals varied from four to 19, and observed and expected heterozygosities ranged from 0.450 to 0.925 and from 0.497 to 0.899, respectively. Nine loci showed no significan...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2008.02286.x

    authors: He T,Krauss SL,Lamont BB

    更新日期:2008-11-01 00:00:00

  • Methods for identifying and interpreting sex-linked SNP markers and carrying out sex assignment: application to thornback ray (Raja clavata).

    abstract::Sex-determining modes remain unknown in numerous species, notably in fishes, in which a variety of modalities have been reported. Additionally, noninvasive individual sexing is problematic for species without external sex attributes or for early life stages, requiring cytogenetic or molecular analyses when sex chromos...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.13225

    authors: Trenkel VM,Boudry P,Verrez-Bagnis V,Lorance P

    更新日期:2020-11-01 00:00:00

  • PhytoREF: a reference database of the plastidial 16S rRNA gene of photosynthetic eukaryotes with curated taxonomy.

    abstract::Photosynthetic eukaryotes have a critical role as the main producers in most ecosystems of the biosphere. The ongoing environmental metabarcoding revolution opens the perspective for holistic ecosystems biological studies of these organisms, in particular the unicellular microalgae that often lack distinctive morpholo...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12401

    authors: Decelle J,Romac S,Stern RF,Bendif el M,Zingone A,Audic S,Guiry MD,Guillou L,Tessier D,Le Gall F,Gourvil P,Dos Santos AL,Probert I,Vaulot D,de Vargas C,Christen R

    更新日期:2015-11-01 00:00:00

  • DNA barcode accumulation curves for understudied taxa and areas.

    abstract::Frequently, the diversity of umbrella taxa is invoked to predict patterns of other, less well-known, life. However, the utility of this strategy has been questioned. We tested whether a phylogenetic diversity (PD) analysis of CO1 DNA barcodes could act as a proxy for standard methods of determining sampling efficiency...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2009.02646.x

    authors: Smith MA,Fernandez-Triana J,Roughley R,Hebert PD

    更新日期:2009-05-01 00:00:00

  • Multiscale resistant kernel surfaces derived from inferred gene flow: An application with vernal pool breeding salamanders.

    abstract::The importance of assessing spatial data at multiple scales when modelling species-environment relationships has been highlighted by several empirical studies. However, no landscape genetics studies have optimized landscape resistance surfaces by evaluating relevant spatial predictors at multiple spatial scales. Here,...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.13089

    authors: Winiarski KJ,Peterman WE,Whiteley AR,McGarigal K

    更新日期:2020-01-01 00:00:00

  • First insights into the transcriptome and development of new genomic tools of a widespread circum-Mediterranean tree species, Pinus halepensis Mill.

    abstract::Aleppo pine (Pinus halepensis Mill.) is a relevant conifer species for studying adaptive responses to drought and fire regimes in the Mediterranean region. In this study, we performed Illumina next-generation sequencing of two phenotypically divergent Aleppo pine accessions with the aims of (i) characterizing the tran...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12232

    authors: Pinosio S,González-Martínez SC,Bagnoli F,Cattonaro F,Grivet D,Marroni F,Lorenzo Z,Pausas JG,Verdú M,Vendramin GG

    更新日期:2014-07-01 00:00:00

  • The impact of contaminants on the accuracy of genome skimming and the effectiveness of exclusion read filters.

    abstract::The ability to detect the identity of a sample obtained from its environment is a cornerstone of molecular ecological research. Thanks to the falling price of shotgun sequencing, genome skimming, the acquisition of short reads spread across the genome at low coverage, is emerging as an alternative to traditional barco...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.13135

    authors: Rachtman E,Balaban M,Bafna V,Mirarab S

    更新日期:2020-05-01 00:00:00

  • Development of nine new microsatellite loci for the American beaver, Castor canadensis (Rodentia: Castoridae), and cross-species amplification in the European beaver, Castor fiber.

    abstract::We developed nine new nuclear dinucleotide microsatellite loci for Castor canadensis. All loci were polymorphic, except for one. The number of alleles ranged from two to four and from five to 12 in populations from Arizona and Wisconsin, respectively. Average heterozygosity ranged from 0.13 to 0.86 per locus. Since cr...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/j.1755-0998.2008.02364.x

    authors: Pelz-Serrano K,Munguia-Vega A,Piaggio AJ,Neubaum M,Munclinger P,Pártl A,VAN Riper Iii C,Culver M

    更新日期:2009-03-01 00:00:00

  • SNP discovery in nonmodel organisms: strand bias and base-substitution errors reduce conversion rates.

    abstract::Single nucleotide polymorphisms (SNPs) have become the marker of choice for genetic studies in organisms of conservation, commercial or biological interest. Most SNP discovery projects in nonmodel organisms apply a strategy for identifying putative SNPs based on filtering rules that account for random sequencing error...

    journal_title:Molecular ecology resources

    pub_type: 杂志文章

    doi:10.1111/1755-0998.12343

    authors: Gonçalves da Silva A,Barendse W,Kijas JW,Barris WC,McWilliam S,Bunch RJ,McCullough R,Harrison B,Hoelzel AR,England PR

    更新日期:2015-07-01 00:00:00