Genotyping success of historical Eurasian lynx (Lynx lynx L.) samples.

abstract：:The spatial signature of microevolutionary processes structuring genetic variation may play an important role in the detection of loci under selection. However, the spatial location of samples has not yet been used to quantify this. Here, we present a new two-step method of spatial outlier detection at the individual ...

journal_title：Molecular ecology resources

authors： Wagner HH,Chávez-Pesqueira M,Forester BR

更新日期：2017-11-01 00:00:00

abstract：:We tested for cross-species amplification of microsatellite loci located throughout the domestic sheep (Ovis aries) genome in two north American mountain ungulates (bighorn sheep, Ovis canadensis, and mountain goats, Oreamnos americanus). We identified 247 new polymorphic markers in bighorn sheep (≥ 3 alleles in one o...

journal_title：Molecular ecology resources

authors： Poissant J,Shafer AB,Davis CS,Mainguy J,Hogg JT,Côté SD,Coltman DW

更新日期：2009-07-01 00:00:00

abstract：:DNA-based identifications have been employed across broad taxonomic ranges and provide an especially useful tool in cases where external identification may be problematic. This study explored the utility of DNA barcoding in resolving skate species found in Atlantic Canadian waters. Most species were clearly resolved, ...

journal_title：Molecular ecology resources

authors： Coulson MW,Denti D,Van Guelpen L,Miri C,Kenchington E,Bentzen P

更新日期：2011-11-01 00:00:00

abstract：:Noninvasive genetic approaches continue to improve studies in molecular ecology, conservation genetics and related disciplines such as forensics and epidemiology. Noninvasive sampling allows genetic studies without disturbing or even seeing the target individuals. Although noninvasive genetic sampling has been used fo...

journal_title：Molecular ecology resources

authors： Beja-Pereira A,Oliveira R,Alves PC,Schwartz MK,Luikart G

更新日期：2009-09-01 00:00:00

abstract：:In noninvasive genetic sampling, when genotyping error rates are high and recapture rates are low, misidentification of individuals can lead to overestimation of population size. Thus, estimating genotyping errors is imperative. Nonetheless, conducting multiple polymerase chain reactions (PCRs) at multiple loci is tim...

journal_title：Molecular ecology resources

authors： Hansen H,Ben-David M,McDonald DB

更新日期：2008-03-01 00:00:00

abstract：:GONe is a user-friendly, Windows-based program for estimating effective size (N(e) ) in populations with overlapping generations. It uses the Jorde-Ryman modification to the temporal method to account for age structure in populations. This method requires estimates of age-specific survival and birth rate and allele fr...

journal_title：Molecular ecology resources

authors： Coombs JA,Letcher BH,Nislow KH

更新日期：2012-01-01 00:00:00

abstract：:The almendro (Dipteryx panamensis, Fabaceae) is a tetraploid tree native to the Atlantic lowland rainforests of Central America. We present nine microsatellite primer pairs amplified in three multiplexed reactions for 549 individuals from four sites in Costa Rica. All loci were polymorphic, ranging from three to 13 al...

journal_title：Molecular ecology resources

authors： Hanson TR,Brunsfeld SJ,Finegan B,Waits LP

更新日期：2008-03-01 00:00:00

abstract：:Ecological understanding of the role of consumer-resource interactions in natural food webs is limited by the difficulty of accurately and efficiently determining the complex variety of food types animals have eaten in the field. We developed a method based on DNA metabarcoding multiplexing and next-generation sequenc...

journal_title：Molecular ecology resources

authors： De Barba M,Miquel C,Boyer F,Mercier C,Rioux D,Coissac E,Taberlet P

更新日期：2014-03-01 00:00:00

abstract：:Sex-linked molecular markers have become valuable tools for understanding sex ratio evolution and sex-specific physiology in pre-reproductive plants. To develop new accurate methods for sexing Distichlis spicata juveniles and nonflowering individuals, we converted a random amplified polymorphic DNA-polymerase chain re...

journal_title：Molecular ecology resources

authors： Eppley SM,O'Quinn R,Brown AL

更新日期：2009-09-01 00:00:00

abstract：:The interbreeding of individuals coming from genetically differentiated but incompletely isolated populations can lead to the formation of admixed populations, having important implications in ecology and evolution. In this simulation study, we evaluate how individual admixture proportions estimated by the software st...

journal_title：Molecular ecology resources

authors： Toyama KS,Crochet PA,Leblois R

更新日期：2020-11-01 00:00:00

abstract：:The importance of assessing spatial data at multiple scales when modelling species-environment relationships has been highlighted by several empirical studies. However, no landscape genetics studies have optimized landscape resistance surfaces by evaluating relevant spatial predictors at multiple spatial scales. Here,...

journal_title：Molecular ecology resources

authors： Winiarski KJ,Peterman WE,Whiteley AR,McGarigal K

更新日期：2020-01-01 00:00:00

abstract：:We implemented multilocus selection in a spatially-explicit, individual-based framework that enables multivariate environmental gradients to drive selection in many loci as a new module for the landscape genetics programs, CDPOP and CDMetaPOP. Our module simulates multilocus selection using a linear additive model, pr...

journal_title：Molecular ecology resources

authors： Landguth EL,Forester BR,Eckert AJ,Shirk AJ,Menon M,Whipple A,Day CC,Cushman SA

更新日期：2020-03-01 00:00:00

abstract：:The emergence of third-generation sequencing (3GS; long-reads) is bringing closer the goal of chromosome-size fragments in de novo genome assemblies. This allows the exploration of new and broader questions on genome evolution for a number of nonmodel organisms. However, long-read technologies result in higher sequenc...

journal_title：Molecular ecology resources

authors： Jaworski CC,Allan CW,Matzkin LM

更新日期：2020-09-01 00:00:00

abstract：:DNA barcoding is a molecular method that rapidly identifies an individual to a known taxon or its closest relative based on a 650-bp fragment of the cytochrome c oxidase subunit I (COI). In this study, DNA barcodes of members of the family Accipitridae, including Haliastur indus (brahminy kite), Haliaeetus leucogaster...

journal_title：Molecular ecology resources

authors： Ong PS,Luczon AU,Quilang JP,Sumaya AM,Ibañez JC,Salvador DJ,Fontanilla IK

更新日期：2011-03-01 00:00:00

abstract：:Species identification based on short sequences of DNA markers, that is, DNA barcoding, has emerged as an integral part of modern taxonomy. However, software for the analysis of large and multilocus barcoding data sets is scarce. The Basic Local Alignment Search Tool (BLAST) is currently the fastest tool capable of ha...

journal_title：Molecular ecology resources

authors： Fan L,Hui JH,Yu ZG,Chu KH

更新日期：2014-07-01 00:00:00

abstract：:To characterize microbiomes and other ecological assemblages, ecologists routinely sequence and compare loci that differ among focal taxa. Counts of these sequences convey information regarding the occurrence and relative abundances of taxa, but provide no direct measure of their absolute abundances, due to the techni...

journal_title：Molecular ecology resources

authors： Harrison JG,John Calder W,Shuman B,Alex Buerkle C

更新日期：2021-01-01 00:00:00

abstract：:Incorporating historical tissues into the study of ecological, conservation and management questions can broaden the scope of population genetic research by enhancing our understanding of evolutionary processes and anthropogenic influences on natural populations. Genotyping historical and low-quality samples has been ...

journal_title：Molecular ecology resources

authors： Smith MJ,Pascal CE,Grauvogel Z,Habicht C,Seeb JE,Seeb LW

更新日期：2011-03-01 00:00:00

abstract：:Mitochondrial DNA (mtDNA) has formed the backbone of phylogeographic research for many years; however, recent trends focus on genome-wide analyses. One method proposed for calibrating inferences from noisy next-generation data, such as RAD sequencing, is to compare these results with analyses of mitochondrial sequence...

journal_title：Molecular ecology resources

authors： Stobie CS,Cunningham MJ,Oosthuizen CJ,Bloomer P

更新日期：2019-01-01 00:00:00

abstract：:We describe a convenient, cost-effective and flexible medium-throughput single nucleotide polymorphism (SNP) genotyping method, Multiplex SNP-SCALE, which enables the simultaneous amplification by polymerase chain reaction (PCR) of up to 25 (or potentially more) loci followed by electrophoresis in an automated DNA seq...

journal_title：Molecular ecology resources

authors： Kenta T,Gratten J,Haigh NS,Hinten GN,Slate J,Butlin RK,Burke T

更新日期：2008-11-01 00:00:00

abstract：:Metabarcoding is increasingly used to assess species diversity by high-throughput sequencing where millions of sequences can be generated in parallel and multiple samples can be analysed in one sequencing run. Generating amplified fragments with a unique sequence identifier ('tag') for each sample is crucial, as it al...

journal_title：Molecular ecology resources

authors： Zizka VMA,Elbrecht V,Macher JN,Leese F

更新日期：2019-07-01 00:00:00

abstract：:Eight polymorphic microsatellite loci were isolated and characterized from a microsatellite DNA-enriched DNA library for the leopard coralgrouper (Plectropomus leopardus Lacepède, 1802), a popular food fish in the East Indies. These loci showed polymorphism information content ranging from 0.493 to 0.854, allele numbe...

journal_title：Molecular ecology resources

authors： Ding SX,Zeng HS,Wang Y,Pan Y,Shi XF

更新日期：2009-11-01 00:00:00

abstract：:The mitochondrial CO1 gene (cytochrome c oxidase I) is a widely accepted metazoan barcode region. In insects, the mitochondrial NADH dehydrogenase subunit 1 (ND1) gene region has proved to be another suitable marker especially for the identification of lower level taxonomic entities such as populations and sister spec...

journal_title：Molecular ecology resources

authors： Bergmann T,Rach J,Damm S,Desalle R,Schierwater B,Hadrys H

更新日期：2013-11-01 00:00:00

abstract：:DNA extraction from minute hymenopterans and their larvae is difficult and challenging because of their small size indicating a low amount of starting material. Hence, 11 DNA extraction methods were compared to determine their efficacy in isolating DNA. Success of each method was scored on a 2% agarose gel after PCR o...

journal_title：Molecular ecology resources

authors： Dittrich-Schröder G,Wingfield MJ,Klein H,Slippers B

更新日期：2012-01-01 00:00:00

abstract：:High-throughput identification technologies provide efficient tools for understanding the ecology and functioning of microorganisms. Yet, these methods have been only rarely used for monitoring and testing ecological hypotheses in plant pathogens and pests in spite of their immense importance in agriculture, forestry ...

journal_title：Molecular ecology resources

authors： Tedersoo L,Drenkhan R,Anslan S,Morales-Rodriguez C,Cleary M

更新日期：2019-01-01 00:00:00

abstract：:Bactrocera cucurbitae (Coquillett) is an important insect pest of melon fruit causing extensive losses of production in Asia and Pacific areas. Twelve polymorphic microsatellite loci were isolated from an enriched DNA library of this species and their amplification characteristics are described. Genetic parameters wer...

journal_title：Molecular ecology resources

authors： Wu Y,Li Z,Wu J

更新日期：2009-09-01 00:00:00

abstract：:Proportional and separate models able to apply different combination of substitution rate matrix (SRM) and among-site rate variation model (ASRVM) to each locus are frequently used in phylogenetic studies of multilocus data. A proportional model assumes that branch lengths are proportional among partitions and a separ...

journal_title：Molecular ecology resources

authors： Tanabe AS

更新日期：2011-09-01 00:00:00

abstract：:In the Alectoris partridges (Phasianidae), hybridization occurs occasionally as a result of the natural breakdown of isolating mechanisms but more frequently as a result of human activity. No genetic record of hybridization is known for the barbary partridge (A. barbara). This species is distributed mostly in North Af...

journal_title：Molecular ecology resources

authors： Barbanera F,Guerrini M,Bertoncini F,Cappelli F,Muzzeddu M,Dini F

更新日期：2011-01-01 00:00:00

abstract：:Single nucleotide polymorphism (SNP) markers were identified and validated for two stingrays species, Potamotrygon motoro and Potamotrygon falkneri, using double digest restriction-site associated DNA (ddRAD) reads using 454-Roche technology. A total of 226 774 reads (65.5 Mb) were obtained (mean read length 289 ± 183...

journal_title：Molecular ecology resources

authors： Cruz VP,Vera M,Pardo BG,Taggart J,Martinez P,Oliveira C,Foresti F

更新日期：2017-05-01 00:00:00

abstract：:Despite the contribution of DNA barcoding towards understanding the biodiversity and distribution of species, the success of COI amplification has been quite variable when it comes to freshwater zooplankton (Elías-Gutiérrez & Valdez-Moreno 2008; Jeffery et al. 2011). Some genera of microcrustaceans seem to be more dif...

journal_title：Molecular ecology resources

authors： Prosser S,Martínez-Arce A,Elías-Gutiérrez M

更新日期：2013-11-01 00:00:00

abstract：:We isolated and characterized 52 novel microsatellite markers from Florida largemouth bass, Micropterus salmoides floridanus, for use in conservation, management and population genetic studies. Markers were assessed in M. s. floridanus from peninsular Florida (n = 30) and averaged eight alleles per locus with observed...

journal_title：Molecular ecology resources

authors： Lutz-Carrillo DJ,Hagen C,Dueck LA,Glenn TC

更新日期：2008-01-01 00:00:00