Abstract:
:The present study applied the PacBio single molecule, real-time sequencing technology (SMRT) in evaluating the quality of silage production. Specifically, we produced four types of Medicago sativa silages by using four different lactic acid bacteria-based additives (AD-I, AD-II, AD-III and AD-IV). We monitored the changes in pH, organic acids (including butyric acid, the ratio of acetic acid/lactic acid, γ-aminobutyric acid, 4-hyroxy benzoic acid and phenyl lactic acid), mycotoxins, and bacterial microbiota during silage fermentation. Our results showed that the use of the additives was beneficial to the silage fermentation by enhancing a general pH and mycotoxin reduction, while increasing the organic acids content. By SMRT analysis of the microbial composition in eight silage samples, we found that the bacterial species number and relative abundances shifted apparently after fermentation. Such changes were specific to the LAB species in the additives. Particularly, Bacillus megaterium was the initial dominant species in the raw materials; and after the fermentation process, Pediococcus acidilactici and Lactobacillus plantarum became the most prevalent species, both of which were intrinsically present in the LAB additives. Our data have demonstrated that the SMRT sequencing platform is applicable in assessing the quality of silage.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Bao W,Mi Z,Xu H,Zheng Y,Kwok LY,Zhang H,Zhang Wdoi
10.1038/srep28358subject
Has Abstractpub_date
2016-06-24 00:00:00pages
28358issn
2045-2322pii
srep28358journal_volume
6pub_type
杂志文章abstract::Capable of generating plasmonic and other effects, gold nanostructures can offer a variety of diagnostic and therapy functionalities for biomedical applications, but conventional chemically-synthesized Au nanomaterials cannot always match stringent requirements for toxicity levels and surface conditioning. Laser-synth...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-48748-3
更新日期:2019-09-09 00:00:00
abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-018-22724-9
更新日期:2018-03-13 00:00:00
abstract::Oxidative stress (OS), mitochondrial dysfunction, and dysregulation of alpha-synuclein (aSyn) homeostasis are key pathogenic factors in Parkinson's disease. Nevertheless, the role of aSyn in mitochondrial physiology remains elusive. Thus, we addressed the impact of aSyn specifically on mitochondrial response to OS in ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep42942
更新日期:2017-02-22 00:00:00
abstract::Boiling histotripsy (BH) is a High Intensity Focused Ultrasound (HIFU) method for precise mechanical disintegration of target tissue using millisecond-long pulses containing shocks. BH treatments with real-time ultrasound (US) guidance allowed by BH-generated bubbles were previously demonstrated ex vivo and in vivo in...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-56658-7
更新日期:2019-12-27 00:00:00
abstract::Regular drusen, an accumulation of material below the retinal pigment epithelium (RPE), have long been established as a hallmark early feature of nonneovascular age-related macular degeneration (AMD). Advances in imaging have expanded the phenotype of AMD to include another extracellular deposit, reticular pseudodruse...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-66355-5
更新日期:2020-06-12 00:00:00
abstract::The concept of brain circuit disorders has been proposed for a variety of neuropsychiatric diseases, characterized by pathological disturbances of neuronal networks including changes in oscillatory signaling of re-entrant cortico-subcortical loops in the basal ganglia system. Parts of this circuitry play a pivotal rol...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-15872-x
更新日期:2017-11-14 00:00:00
abstract::Accumulating evidence indicates the association between obesity and lung function. However, no previous study has examined whether obesity affects lung function in normal weight participants with high body fat. We hypothesized that subjects with normal weight obesity (NWO) were inversely associated with lung function ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-38804-3
更新日期:2019-06-20 00:00:00
abstract::Dehydroascorbate reductase (DHAR) is a key enzyme involved in the recycling of ascorbate, which catalyses the glutathione (GSH)-dependent reduction of oxidized ascorbate (dehydroascorbate, DHA). As a result, DHAR regenerates a pool of reduced ascorbate and detoxifies reactive oxygen species (ROS). In previous experime...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep19498
更新日期:2016-01-18 00:00:00
abstract::Serum and cerebrospinal fluid (CSF) levels of α-fetoprotein and β-subunit of human chorionic gonadotropin are used as biomarkers for the management of central nervous system (CNS) germ cell tumors (GCTs). However, additional discriminating biomarkers are required. Especially, biomarkers to differentiate non-germinomat...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-71161-0
更新日期:2020-08-31 00:00:00
abstract::Ginseng rusty root (GRR) symptom is one of the primary diseases of ginseng. There has been a problem of ginseng rusty root, leading to a severe decline in the quality of ginseng. To clarify the relationship between root symptoms of ginseng rust and soil, the physical and chemical properties, enzyme activity, community...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-71024-8
更新日期:2020-09-25 00:00:00
abstract::Coleoptera (beetles) is a massively successful order of insects, distinguished by their evolutionarily modified forewings called elytra. These structures are often presumed to have been a major driving force for the successful radiation of this taxon, by providing beetles with protection against a variety of harsh env...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep34813
更新日期:2016-10-06 00:00:00
abstract::Increasing population has posed insurmountable challenges to agriculture in the provision of future food security, particularly in the Middle East and North Africa (MENA) region where biophysical conditions are not well-suited for agriculture. Iran, as a major agricultural country in the MENA region, has long been in ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-08066-y
更新日期:2017-08-09 00:00:00
abstract::The spreading of cooperation in structured population is a challenging problem which can be observed at different scales of social and biological organization. Generally, the problem is studied by evaluating the chances that few initial invading cooperators, randomly appearing in a network, can lead to the spreading o...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-80770-8
更新日期:2021-01-13 00:00:00
abstract::Microscopic plastic (microplastic, 0.1 µm-5 mm) is a widespread pollutant impacting upon aquatic ecosystems across the globe. Environmental sampling has revealed synthetic fibers are prevalent in seawater, sediments and biota. However, microplastic fibers are rarely used in laboratory studies as they are unavailable f...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep34519
更新日期:2016-10-03 00:00:00
abstract::A series of three remarkable complexes [PMo12O40]@[Cu6O(TZI)3(H2O)9]4·OH·31H2O (H3TZI = 5-tetrazolylisophthalic acid; denoted as HLJU-1, HLJU = Heilongjiang University), [SiMo12O40]@[Cu6O(TZI)3(H2O)9]4·32H2O (denoted as HLJU-2), and [PW12O40]@[Cu6O(TZI)3(H2O)6]4·OH·31H2O (denoted as HLJU-3) have been isolated by using...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep25595
更新日期:2016-05-09 00:00:00
abstract::Neoadjuvant systemic treatment is increasingly being integrated in the standard treatment of pancreatic ductal adenocarcinoma (PDAC) patients to improve oncological outcomes. Current available imaging techniques remain unreliable in assessing response to therapies, as they cannot distinguish between (vital) tumor tiss...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-73242-6
更新日期:2020-10-01 00:00:00
abstract::Patients who survive influenza A (H7N9) virus infection are at risk of physical and psychological complications of lung injury and multi-organ dysfunction. However, there were no prospectively individualized assessments of physiological, functional and quality-of-life measures after hospital discharge. The current stu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-17497-6
更新日期:2017-12-08 00:00:00
abstract::We design a novel long-range hybrid wedge plasmonic (LRHWP) waveguide composed of two identical dielectric nanowires symmetrically placed on two opposed wedges of a diamond shaped metal wire. With strong coupling between the dielectric nanowire mode and long-range surface plasmon polariton (SPP) mode, both deep subwav...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep06870
更新日期:2014-11-03 00:00:00
abstract::Tissue damage induces rapid recruitment of leukocytes and changes in the transcriptional landscape that influence wound healing. However, the cell-type specific transcriptional changes that influence leukocyte function and tissue repair have not been well characterized. Here, we employed translating ribosome affinity ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-72750-9
更新日期:2020-09-24 00:00:00
abstract::Although diatoms have been extensively studied as bioreactors, only a limited number of efficient gene promoters are available. Therefore, the development of new endogenous promoters is important for the heterologous production of a variety of recombinant proteins. Herein, we identified the most abundant secreted prot...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-45786-9
更新日期:2019-07-09 00:00:00
abstract::Balloon whisk-like and flower-like SiOx tubes with well-dispersed Sn and joining countless SiOx loops together induce intense luminescence characteristics in substrate materials. Our synthetic technique called "direct substrate growth" is based on pre-contamination of the surroundings without the intended catalyst and...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep30901
更新日期:2016-08-01 00:00:00
abstract::Dumbbell probe (DP) attracts increasing interests in rolling circle amplification (RCA). A universal DP production method through cleavage-ligation of hairpin was proposed and optimized. The production is characterized by restriction endonuclease (RE)-induced cleavage ends ligation. It has the advantage of phosphoryla...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep29229
更新日期:2016-07-07 00:00:00
abstract::A host of hormonal-metabolic alterations take place following exposure of cardiomyocytes to hypoxia and other noxious stimuli. Here, we demonstrate that exposure of cultured rat cardiomyocytes to lipopolysaccharide (LPS) resulted in upregulation (~1.5 fold) of oxidized low-density lipoprotein receptor-1 (LOX-1). There...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-55694-7
更新日期:2019-12-17 00:00:00
abstract::At the species level, plants can respond to climate changes by changing their leaf traits; however, there is scant information regarding the responses of morphological, physiological and anatomical traits of plant functional types (PFTs) to aridity. Herein, the leaf traits of five PFTs representing 17 plant species in...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep40900
更新日期:2017-01-20 00:00:00
abstract::The potential of Massively Parallel Sequencing (MPS) technology to vastly expand the capabilities of human identification led to the emergence of different MPS platforms that use forensically relevant genetic markers. Two of the MPS platforms that are currently available are the MiSeq® FGx™ Forensic Genomics System (I...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-00510-3
更新日期:2017-03-24 00:00:00
abstract::Delivering liquid through the void spaces in porous metals is a daunting challenge for a variety of emerging interface technologies ranging from battery electrodes to evaporation surfaces. Hydraulic transport characteristics of well-ordered porous media are governed by the pore distribution, porosity, and morphology. ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-10791-3
更新日期:2017-09-05 00:00:00
abstract::Our understanding of the structure and regulation of Plasmodium vivax genes is limited by our inability to grow the parasites in long-term in vitro cultures. Most P. vivax studies must therefore rely on patient samples, which typically display a low proportion of parasites and asynchronous parasites. Here, we present ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-07275-9
更新日期:2017-08-10 00:00:00
abstract::BiFeO3 is one of the most promising multiferroic materials but undergoes two major drawbacks: low dielectric susceptibility and high dielectric loss. Here we report high in-plane dielectric permittivity (ε' ∼2500) and low dielectric loss (tan δ < 0.01) obtained on Bi0.95Y0.05FeO3 films epitaxially grown on SrTiO3 (001...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep25535
更新日期:2016-05-09 00:00:00
abstract::Mitochondria are organelles that govern energy supply and control cell death. Mitochondria also express bacterial features, such as the presence of inner membrane cardiolipin and a circular genome rich in hypomethylated CpG motifs. While mitochondrial extrusion by damaged organs or activated cells is thought to trigge...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-40900-3
更新日期:2019-03-14 00:00:00
abstract::Several carbon dots doping with diferent elements (Ca, Ag, Au) were fabricated and their catalytic properties had been investigated in this paper. It was found that the Ag-doped carbon dots (CDAg) had played a role of mimic enzyme on the reaction of HAuCl4-H2O2 and generated nanogold particles with surface enhanced Ra...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-46426-y
更新日期:2019-07-10 00:00:00