Abstract:
:Advanced personalized medical diagnostics depend on the availability of high-quality biological samples. These are typically biofluids, such as blood, saliva, or urine; and their collection and storage is critical to obtain reliable results. Without proper temperature regulation, protein biomarkers in particular can degrade rapidly in blood samples, an effect that ultimately compromises the quality and reliability of laboratory tests. Here, we present the use of silk fibroin as a solid matrix to encapsulate blood analytes, protecting them from thermally induced damage that could be encountered during nonrefrigerated transportation or freeze-thaw cycles. Blood samples are recovered by simple dissolution of the silk matrix in water. This process is demonstrated to be compatible with a number of immunoassays and provides enhanced sample preservation in comparison with traditional air-drying paper approaches. Additional processing can remediate interactions with conformational structures of the silk protein to further enhance blood stabilization and recovery. This approach can provide expanded utility for remote collection of blood and other biospecimens empowering new modalities of temperature-independent remote diagnostics.
journal_name
Proc Natl Acad Sci U S Aauthors
Kluge JA,Li AB,Kahn BT,Michaud DS,Omenetto FG,Kaplan DLdoi
10.1073/pnas.1602493113subject
Has Abstractpub_date
2016-05-24 00:00:00pages
5892-7issue
21eissn
0027-8424issn
1091-6490pii
1602493113journal_volume
113pub_type
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