Mating signal and DNA penetration deficiency in conjugation between male Escherichia coli and minicells.

abstract：:Phalloidin, a toxic bicyclic peptide of rapid action from the toadstool, Amanita phalloides, gives rise to polymerization of G-actin to filamentous structures (Ph-actin) in a medium of low ionic strength. Ph-actin closely resembles the microfilaments found in liver membrane fractions (Ph-filaments) after in vivo or in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lengsfeld AM,Löw I,Wieland T,Dancker P,Hasselbach W

更新日期：1974-07-01 00:00:00

abstract：:Calendar date of the beginning of the growing season at high altitude in the Colorado Rocky Mountains is variable but has not changed significantly over the past 25 years. This result differs from growing evidence from low altitudes that climate change is resulting in a longer growing season, earlier migrations, and e...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Inouye DW,Barr B,Armitage KB,Inouye BD

更新日期：2000-02-15 00:00:00

abstract：:The localization of calmodulin, a calcium-dependent modulator of many enzymes, was studied in rat liver, skeletal muscle, and adrenal slices. Calmodulin is found in liver cytoplasm, nucleus, and plasma membrane. Much of the cytoplasmic calmodulin is associated with glycogen particles presumably bound to enzymes involv...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Harper JF,Cheung WY,Wallace RW,Huang HL,Levine SN,Steiner AL

更新日期：1980-01-01 00:00:00

abstract：:The equations of motion of the coupled electron-phonon system are integrated in real time for the model of polyacetylene recently proposed. To illustrate the physical behavior of this nonlinear system we consider the time evolution starting from three physically relevant configurations: (i) end generated soliton, (ii)...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Su WP,Schrieffer JR

更新日期：1980-10-01 00:00:00

abstract：:Eukaryotic initiator proteins form origin recognition complexes (ORCs) that bind to replication origins during most of the cell cycle and direct assembly of prereplication complexes (pre-RCs) before the onset of S phase. In the eubacterium Escherichia coli, there is a temporally similar nucleoprotein complex comprisin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miller DT,Grimwade JE,Betteridge T,Rozgaja T,Torgue JJ,Leonard AC

更新日期：2009-11-03 00:00:00

abstract：:Although the transport sector is responsible for a large and growing share of global emissions affecting climate, its overall contribution has not been quantified. We provide a comprehensive analysis of radiative forcing from the road transport, shipping, aviation, and rail subsectors, using both past- and forward-loo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fuglestvedt J,Berntsen T,Myhre G,Rypdal K,Skeie RB

更新日期：2008-01-15 00:00:00

abstract：:Shewanella species are renowned for their respiratory versatility, including their ability to respire poorly soluble substrates by using enzymatic machinery that is localized to the outside of the cell. The ability to engage in "extracellular respiration" to date has focused primarily on respiration of minerals. Here,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gralnick JA,Vali H,Lies DP,Newman DK

更新日期：2006-03-21 00:00:00

abstract：:Protection of poikilothermic animals from seasonal cold is widely regarded as being causally linked to changes in the unsaturation of membrane phospholipids, yet in animals this proposition remains formally untested. We have now achieved this by the genetic manipulation of lipid biosynthesis of Caenorhabditis elegans ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Murray P,Hayward SA,Govan GG,Gracey AY,Cossins AR

更新日期：2007-03-27 00:00:00

abstract：:The UV photodissociation kinetics of tryptophan amino acid, Trp, attached to the membrane of bacteria, Escherichia coli and Bacillus subtilis, have been studied by means of normal and synchronous fluorescence. Our experimental data suggest that the fluorescence intensity of Trp increases during the first minute of irr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li R,Dhankhar D,Chen J,Cesario TC,Rentzepis PM

更新日期：2019-09-17 00:00:00

abstract：:The sloppy paired (slp) locus consists of two genes, slp1 and slp2, both of which encode proteins containing a forkhead domain (a DNA-binding motif). Previous work has shown that a severe segmentation phenotype is obtained only when both slp genes are deleted. Here we examine the functional redundancy of the locus in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cadigan KM,Grossniklaus U,Gehring WJ

更新日期：1994-07-05 00:00:00

abstract：:A previously undescribed toothless flying reptile from northeastern China, Nemicolopterus crypticus gen. et sp. nov., was discovered in the lacustrine sediments of the Early Cretaceous Jiufotang Formation, western Liaoning, China. The specimen consists of an almost complete articulated skeleton (IVPP V14377) and, desp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang X,Kellner AW,Zhou Z,Campos Dde A

更新日期：2008-02-12 00:00:00

abstract：:We examined the contributions of the human pulvinar to goal directed selection of visual targets in 3 patients with chronic, unilateral lesions involving topographic maps in the ventral pulvinar. Observers completed 2 psychophysical tasks in which they discriminated the orientation of a lateralized target grating in t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Snow JC,Allen HA,Rafal RD,Humphreys GW

更新日期：2009-03-10 00:00:00

abstract：:The family of WD40-repeat (WDR) proteins is one of the largest in eukaryotes, but little is known about their function in brain development. Among 26 WDR genes assessed, we found 7 displaying a major impact in neuronal morphology when inactivated in mice. Remarkably, all seven genes showed corpus callosum defects, inc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kannan M,Bayam E,Wagner C,Rinaldi B,Kretz PF,Tilly P,Roos M,McGillewie L,Bär S,Minocha S,Chevalier C,Po C,Sanger Mouse Genetics Project.,Chelly J,Mandel JL,Borgatti R,Piton A,Kinnear C,Loos B,Adams DJ,Hérault Y,

更新日期：2017-10-31 00:00:00

abstract：:The bioluminescent bacterium Vibrio fischeri initiates a specific, persistent symbiosis in the light organ of the squid Euprymna scolopes. During the early stages of colonization, V. fischeri is exposed to host-derived nitric oxide (NO). Although NO can be both an antimicrobial component of innate immunity and a key s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang Y,Dufour YS,Carlson HK,Donohue TJ,Marletta MA,Ruby EG

更新日期：2010-05-04 00:00:00

abstract：:The replisome, the multiprotein system responsible for genome duplication, is a highly dynamic complex displaying a large number of different enzyme activities. Recently, the Saccharomyces cerevisiae minimal replication reaction has been successfully reconstituted in vitro. This provided an opportunity to uncover the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lewis JS,Spenkelink LM,Schauer GD,Hill FR,Georgescu RE,O'Donnell ME,van Oijen AM

更新日期：2017-10-03 00:00:00

abstract：:The recombinant plasmid p102 based on pBR322 carrying approximately equal to 50% of the replicator proximal early region of simian virus 40 (SV40) DNA, including the viral origin of replication, has been constructed. It lacks a major part of the large tumor (T) antigen 3'-coding region, the T-antigen termination codon...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Horst J,Weckler C,Jacob E,Aulehla-Scholz C,Deppert W

更新日期：1982-08-01 00:00:00

abstract：:Most guanine nucleotide binding protein (G protein)-coupled receptors have a conserved cysteine in the C-terminal cytoplasmic tail near the seventh transmembrane spanning region. This cysteine is known to be palmitoylated in rhodopsin, the beta 2-adrenergic receptor (beta 2AR) and the alpha 2A-adrenergic receptor (alp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eason MG,Jacinto MT,Theiss CT,Liggett SB

更新日期：1994-11-08 00:00:00

abstract：:3-Hydroxykynurenine (3-HK) is a tryptophan metabolite whose level in the brain is markedly elevated under several pathological conditions, including Huntington disease and human immunodeficiency virus infection. Here we demonstrate that micromolar concentrations (1-100 microM) of 3-HK cause cell death in primary neuro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Okuda S,Nishiyama N,Saito H,Katsuki H

更新日期：1996-10-29 00:00:00

abstract：:The mechanical properties of engineering structures continuously weaken during service life because of material fatigue or degradation. By contrast, living organisms are able to strengthen their mechanical properties by regenerating parts of their structures. For example, plants strengthen their cell structures by tra...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yu K,Feng Z,Du H,Xin A,Lee KH,Li K,Su Y,Wang Q,Fang NX,Daraio C

更新日期：2021-01-19 00:00:00

abstract：:Besides ethanol, acetic acid is produced in naturally fermenting sweet resources and is a significant environmental stress for fruit-breeding Drosophila populations and species. Although not related to the presence of an active alcohol dehydrogenase, adult acetic acid tolerance was found to correlate with ethanol tole...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chakir M,Peridy O,Capy P,Pla E,David JR

更新日期：1993-04-15 00:00:00

abstract：:RNA was synthesized in vitro from a T3 DNA template by T3 RNA polymerase and subsequently separated into seven discrete size classes (molecular weights ranging between 0.21 x 10(6) and 6.2 x 10(6)) by electrophoresis in polyacrylamide slab gels. RNase T1-generated 3'-terminal oligonucleotide fragments were then select...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Majumder HK,Maitra U,Rosenberg M

更新日期：1979-10-01 00:00:00

abstract：:Microbial fatty acid derivatives are emerging as promising alternatives to fossil fuel derived transportation fuels. Among bacterial fatty acid synthases (FAS), the Escherichia coli FAS is perhaps the most well studied, but little is known about its steady-state kinetic behavior. Here we describe the reconstitution of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yu X,Liu T,Zhu F,Khosla C

更新日期：2011-11-15 00:00:00

abstract：:Understanding modulation of memory, as well as the mechanisms underlying memory formation, has become a key issue in neuroscience research. Previously, we found that the formation of long-term, but not short-term, memory for a nonassociative form of learning, sensitization, was modulated by the circadian clock in the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lyons LC,Rawashdeh O,Katzoff A,Susswein AJ,Eskin A

更新日期：2005-08-30 00:00:00

abstract：:Upon release from the anther, pollen grains of angiosperm flowers are exposed to a dry environment and dehydrate. To survive this process, pollen grains possess a variety of physiological and structural adaptations. Perhaps the most striking of these adaptations is the ability of the pollen wall to fold onto itself to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Katifori E,Alben S,Cerda E,Nelson DR,Dumais J

更新日期：2010-04-27 00:00:00

abstract：:Functional transcription elongation complexes can be formed by adding RNA polymerase in trans to a preformed nucleic acid construct. This construct consists of a double-stranded DNA fragment that contains a noncomplementary (permanent DNA bubble) region into which an RNA primer oligonucleotide has been hybridized. By ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Daube SS,Hart CR,von Hippel PH

更新日期：1994-09-27 00:00:00

abstract：:Sliding at a quasi-statically loaded frictional interface can occur via macroscopic slip events, which nucleate locally before propagating as rupture fronts very similar to fracture. We introduce a microscopic model of a frictional interface that includes asperity-level disorder, elastic interaction between local slip...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： de Geus TWJ,Popović M,Ji W,Rosso A,Wyart M

更新日期：2019-11-26 00:00:00

abstract：:Pathways and enzymes of fatty acid synthase-mediated, long-even-chain (generally C16-C20) fatty acid synthesis are well studied, and general metabolism involved in short-chain (C4-C7) fatty acid biosynthesis is also understood. In contrast, mechanisms of medium-chain (C8-C14) fatty acid synthesis are unclear. Recent w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kroumova AB,Xie Z,Wagner GJ

更新日期：1994-11-22 00:00:00

abstract：:Release of formylmethionine from the reticulocyte ribosomal substrate, f[(3)H]Met-tRNA.ribosome, is promoted by reticulocyte release factor (RF). The initial rate of this reaction is stimulated by GTP but inhibited by GDPCP. Formation of an RF.UA[(3)H]A(2).ribosome complex is a measure of the binding of reticulocyte R...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tate WP,Beaudet AL,Caskey CT

更新日期：1973-08-01 00:00:00

abstract：:Albumin Redhill is an electrophoretically slow genetic variant of human serum albumin that does not bind 63Ni2+ and has a molecular mass 2.5 kDa higher than normal albumin. Its inability to bind Ni2+ was explained by the finding of an additional residue of Arg at position -1. This did not explain the molecular basis o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brennan SO,Myles T,Peach RJ,Donaldson D,George PM

更新日期：1990-01-01 00:00:00

abstract：:Nitric oxide (NO) mediates several biological actions, including relaxation of blood vessels, cytotoxicity of activated macrophages, and formation of cGMP by activation of glutamate receptors in cerebellar slices. Nitric oxide synthase (EC 1.14.23.-) immunoreactivity is colocalized with nicotinamide adenine di-nucleot...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dawson VL,Dawson TM,London ED,Bredt DS,Snyder SH

更新日期：1991-07-15 00:00:00