Thy1+IL-7+ lymphatic endothelial cells in iBALT provide a survival niche for memory T-helper cells in allergic airway inflammation.

abstract：:Recoverin, a recently discovered member of the EF-hand superfamily of Ca(2+)-binding proteins, serves as a Ca2+ sensor in vision. The amino terminus of the protein from retinal rod cells contains a covalently attached myristoyl or related N-acyl group. We report here studies of unmyristoylated and myristoylated recomb...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zozulya S,Stryer L

更新日期：1992-12-01 00:00:00

abstract：:Recent studies on proteins whose N and C termini are in close proximity have demonstrated that folding of polypeptide chains and assembly of oligomers can be accomplished with circularly permuted chains. As yet no methodical study has been conducted to determine how extensively new termini can be introduced and where ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Graf R,Schachman HK

更新日期：1996-10-15 00:00:00

abstract：:Microorganisms modify rates and mechanisms of chemical and physical weathering and clay growth, thus playing fundamental roles in soil and sediment formation. Because processes in soils are inherently complex and difficult to study, we employ a model based on the lichen-mineral system to identify the fundamental inter...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Banfield JF,Barker WW,Welch SA,Taunton A

更新日期：1999-03-30 00:00:00

abstract：:The functional significance of the portal vascular link between the beta cells of the pancreatic islets and the liver has not been established. Previous studies indicated that insulin does not acutely regulate glucose metabolism by a direct hepatic effect. More recent observations suggest that the role of insulin in r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Griffen SC,Russell SM,Katz LS,Nicoll CS

更新日期：1987-10-01 00:00:00

abstract：:In the mouse, axons of olfactory sensory neurons (OSNs) that express the same odorant receptor (OR) gene coalesce into one or a few glomeruli in the olfactory bulb. The positions of OR-specific glomeruli are traditionally described as stereotyped. Here, we have assessed quantitatively the positions of OR-specific glom...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zapiec B,Mombaerts P

更新日期：2015-10-27 00:00:00

abstract：:AK032317 is the GenBank accession no. of a full-length RIKEN mouse cDNA. It encodes a putative variant of the C3-type TRPC (transient receptor potential channel) that differs from the previously cloned murine TRPC3 cDNA in that it has a 5' extension stemming from inclusion of an additional exon (exon 0). The extended ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yildirim E,Kawasaki BT,Birnbaumer L

更新日期：2005-03-01 00:00:00

abstract：:There is currently an unmet need for versatile techniques to monitor the assembly and dynamics of ternary complexes in live cells. Here we describe bioluminescence resonance energy transfer with fluorescence enhancement by combined transfer (BRETFect), a high-throughput technique that enables robust spectrometric dete...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cotnoir-White D,El Ezzy M,Boulay PL,Rozendaal M,Bouvier M,Gagnon E,Mader S

更新日期：2018-03-13 00:00:00

abstract：:Cajal-Retzius (CR) cells are early-generated transient neurons and are important in the regulation of cortical neuronal migration and cortical laminar formation. Molecular entities characterizing the CR cell identity, however, remain largely elusive. We purified mouse cortical CR cells expressing GFP to homogeneity by...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yamazaki H,Sekiguchi M,Takamatsu M,Tanabe Y,Nakanishi S

更新日期：2004-10-05 00:00:00

abstract：:The effect of RNA secondary structure on rho-independent and rho-dependent termination of transcription of T3 DNA by Escherichia coli RNA polymerase has been studied by incorporating, into nascent transcripts, base analogs that lead to altered base-pairing properties. A guanine --> hypoxanthine substitution, with atte...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Adhya S,Sarkar P,Valenzuela D,Maitra U

更新日期：1979-04-01 00:00:00

abstract：:A complex of ribosomal DNA with RNA has been isolated from ovaries of metamorphosing tadpoles of Xenopus laevis. The complex is disrupted by treatments that destroy hydrogen bonds, and the sedimentation of the DNA within the complex is sensitive to RNase. We suggest that the RNA-ribosomal DNA complex is an intermediat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brown RD,Tocchini-Valentini GP

更新日期：1972-07-01 00:00:00

abstract：:Neurogenesis has recently been observed in the adult human brain, suggesting the possibility of endogenous neural repair. However, the augmentation of neurogenesis in the adult human brain in response to neuronal cell loss has not been demonstrated. This study was undertaken to investigate whether neurogenesis occurs ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Curtis MA,Penney EB,Pearson AG,van Roon-Mom WM,Butterworth NJ,Dragunow M,Connor B,Faull RL

更新日期：2003-07-22 00:00:00

abstract：:It has been postulated that, in the adult visual cortex, visual inputs modulate levels of mRNAs coding for neurotransmitter receptors in an activity-dependent manner. To investigate this possibility, we performed a monocular enucleation in adult rabbits and, 15 days later, collected their left and right visual cortice...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nguyen QT,Matute C,Miledi R

更新日期：1998-03-17 00:00:00

abstract：:The DNA-binding domain of c-Myb consists of three homologous tandem repeats of 52 amino acids. The structure of the third (C-terminal) repeat obtained by NMR analysis has a conformation related to the helix-turn-helix motif. To identify the role of each repeat in the sequence recognition of DNA, we analyzed specific i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tanikawa J,Yasukawa T,Enari M,Ogata K,Nishimura Y,Ishii S,Sarai A

更新日期：1993-10-15 00:00:00

abstract：:Unprecedented population declines and extinctions because of human activities, combined with a growing recognition that such losses affect the stability of ecosystems, underscore the need to better understand how populations persist naturally. We provide field experimental evidence that high biodiversity-in particular...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carr MH,Anderson TW,Hixon MA

更新日期：2002-08-20 00:00:00

abstract：:Two molecular forms of pyrophosphate-dependent phosphofructokinase (PP(i)-PFK; pyrophosphate:D-fructose-6-phosphate 1-phosphotransferase; EC 2.7.1.90) have been found whose activity depends upon association and dissociation characteristics regulated by fructose 2,6-bisphosphate (Fru-2,6-P(2)). PP(i)-PFK was purified 2...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu MX,Smyth DA,Black CC

更新日期：1984-08-01 00:00:00

abstract：:Amplitude-modulated phosphorescence/microwave double-resonance (AM-PMDR) spectra are reported for complexes of methylmercury(II) cation, designated CH3Hg(II), with tryptophan and glyceraldehyde-3-phosphate dehydrogenase (GPDHase; from rabbit muscle). Wavelength shifts are observed in the AM-PMDR spectra of CH3Hg(II)-t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Davis JM,Maki AH

更新日期：1982-07-01 00:00:00

abstract：:Purine nucleoside analogs modified by replacement of the nitrogen atom at the 3 position by a CH group give a characteristic circular dichroism curve that is not substantially modified by chemical substitution at the 8 position. Since it is rather well established that 8-substituted purine nucleosides are predominantl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miles DW,Townsend LB,Redington P,Eyring H

更新日期：1976-07-01 00:00:00

abstract：:Herpesvirus saimiri (HVS) is the prototype member of a distinctive subset of lymphotropic herpesviruses (the gamma 2 subgroup) with A+T-rich coding sequences. In this paper, we show that cells productively infected with HVS contain high concentrations of a virus-specified thymidylate synthase (5,10-methylenetetrahydro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Honess RW,Bodemer W,Cameron KR,Niller HH,Fleckenstein B,Randall RE

更新日期：1986-06-01 00:00:00

abstract：:T follicular helper (Tfh) and regulatory (Tfr) cells are terminally differentiated cells found in germinal centers (GCs), specialized secondary lymphoid organ structures dedicated to antibody production. As such, follicular T (Tfol) cells are supposed to be specific for immunizing antigens, which has been reported for...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ritvo PG,Saadawi A,Barennes P,Quiniou V,Chaara W,El Soufi K,Bonnet B,Six A,Shugay M,Mariotti-Ferrandiz E,Klatzmann D

更新日期：2018-09-18 00:00:00

abstract：:One of the ultimate objectives of de novo protein design is to realize systems capable of catalyzing redox reactions on substrates. This goal is challenging as redox-active proteins require design considerations for both the reduced and oxidized states of the protein. In this paper, we describe the spectroscopic chara...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tegoni M,Yu F,Bersellini M,Penner-Hahn JE,Pecoraro VL

更新日期：2012-12-26 00:00:00

abstract：:Tubulin cofactor B (TCoB) plays an important role in microtubule dynamics by facilitating the dimerization of alpha- and beta-tubulin. Recent evidence suggests that p21-activated kinase 1 (Pak1), a major signaling nodule in eukaryotic cells, phosphorylates TCoB on Ser-65 and Ser-128 and plays an essential role in micr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rayala SK,Martin E,Sharina IG,Molli PR,Wang X,Jacobson R,Murad F,Kumar R

更新日期：2007-12-04 00:00:00

abstract：:Molecular analyses of Na,K-ATPase abundance and alpha-subunit isoform distribution were performed to determine whether pump expression varies at different points through the thickness of the circular layer of colonic smooth muscle. The mRNA and polypeptides of Na,K-ATPase alpha 1 and beta subunits were twice as abunda...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Burke EP,Sanders KM,Horowitz B

更新日期：1991-03-15 00:00:00

abstract：:The nodZ gene, which is present in various soil bacteria such as Bradyrhizobium japonicum, Azorhizobium caulinodans, and Rhizobium loti, is involved in the addition of a fucosyl residue to the reducing N-acetylglucosamine residue of lipochitin oligosaccharide (LCO) signal molecules. Using an Escherichia coli strain th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Quinto C,Wijfjes AH,Bloemberg GV,Blok-Tip L,López-Lara IM,Lugtenberg BJ,Thomas-Oates JE,Spaink HP

更新日期：1997-04-29 00:00:00

abstract：:Chronic inflammation is a major risk factor for colorectal cancer. The p38/MAPKAP Kinase 2 (MK2) kinase axis controls the synthesis of proinflammatory cytokines that mediate both chronic inflammation and tumor progression. Blockade of this pathway has been previously reported to suppress inflammation and to prevent co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Suarez-Lopez L,Sriram G,Kong YW,Morandell S,Merrick KA,Hernandez Y,Haigis KM,Yaffe MB

更新日期：2018-05-01 00:00:00

abstract：:A change in the conformation of the active site of scallop myosin under the influence of regulatory amounts of Ca2+ has been identified by use of the ADP photoaffinity analog 2-[(4-azido-2-nitrophenyl)amino]ethyl diphosphate (NANDP). NANDP, trapped at the active site with Mn2+ and vanadate, photolabeled preferentially...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kerwin BA,Yount RG

更新日期：1993-01-01 00:00:00

abstract：:The crystal structure of the decapeptide Boc-Aib-Glu(OBzl)-Leu-Aib-Ala-Leu-Aib-Ala-Lys(Z)-Aib-OMe (where Aib is alpha-aminoisobutyryl, Boc is t-butoxycarbonyl, OBzl is benzyl ester, and Z is benzyloxycarbonyl) illustrates a parallel zipper arrangement of interacting helical peptide columns. Head-to-tail NH...OC hydrog...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Karle IL,Flippen-Anderson JL,Uma K,Balaram P

更新日期：1990-10-01 00:00:00

abstract：:Levels and subcellular distribution of connexin 43 (Cx43), a gap junction protein, were studied in hamster leukocytes before and after activation with endotoxin (lipopolysaccharide, LPS) both in vitro and in vivo. Untreated leukocytes did not express Cx43. However, Cx43 was clearly detectable by indirect immunofluores...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jara PI,Boric MP,Sáez JC

更新日期：1995-07-18 00:00:00

abstract：:We demonstrate that the receptor binding moiety of Escherichia coli heat-labile enterotoxin (EtxB) can completely prevent autoimmune disease in a murine model of arthritis. Injection of male DBA/1 mice at the base of the tail with type II collagen in the presence of complete Freund's adjuvant normally leads to arthrit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Williams NA,Stasiuk LM,Nashar TO,Richards CM,Lang AK,Day MJ,Hirst TR

更新日期：1997-05-13 00:00:00

abstract：:Repetitive sequences are hotspots of evolution at multiple levels. However, due to difficulties involved in their assembly and analysis, the role of repeats in tumor evolution is poorly understood. We developed a rigorous motif-based methodology to quantify variations in the repeat content, beyond microsatellites, in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Persi E,Prandi D,Wolf YI,Pozniak Y,Barnabas GD,Levanon K,Barshack I,Barbieri C,Gasperini P,Beltran H,Faltas BM,Rubin MA,Geiger T,Koonin EV,Demichelis F,Horn D

更新日期：2019-08-20 00:00:00

abstract：:The molecular and cellular basis of selective motor neuron (MN) vulnerability in amyotrophic lateral sclerosis (ALS) is not known. In genetically distinct mouse models of familial ALS expressing mutant superoxide dismutase-1 (SOD1), TAR DNA-binding protein 43 (TDP-43), and fused in sarcoma (FUS), we demonstrate select...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lalancette-Hebert M,Sharma A,Lyashchenko AK,Shneider NA

更新日期：2016-12-20 00:00:00