当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Photolabeling evidence for calcium-induced conformational changes at the ATP binding site of scallop myosin.

Photolabeling evidence for calcium-induced conformational changes at the ATP binding site of scallop myosin.

Abstract：

:A change in the conformation of the active site of scallop myosin under the influence of regulatory amounts of Ca2+ has been identified by use of the ADP photoaffinity analog 2-[(4-azido-2-nitrophenyl)amino]ethyl diphosphate (NANDP). NANDP, trapped at the active site with Mn2+ and vanadate, photolabeled preferentially Arg-128 of the heavy chain in the absence of added Mg2+ and Ca2+ [Kerwin, B. & Yount, R. (1992) Bioconjugate Chem. 3, 328-336]. However, addition of 2 mM Mg2+ and regulatory amounts of Ca2+ (0.01-1 microM) shifted the predominant labeling to Cys-198 of the heavy chain in a Ca(2+)-dependent manner. This Ca(2+)-dependent change in the photolabeling pattern was absent when the regulatory light chains were removed or when the unregulated head (subfragment 1) was examined under similar conditions. These results demonstrate that both Arg-128 and Cys-198 are part of the purine binding site which undergoes a conformational change in response to Ca2+ binding to the regulatory domain.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Kerwin BA,Yount RG

doi

10.1073/pnas.90.1.35

subject

Has Abstract

pub_date

1993-01-01 00:00:00

pages

35-9

issue

1

eissn

0027-8424

issn

1091-6490

journal_volume

90

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Molecular and biological changes in the cold-adapted "master strain" A/AA/6/60 (H2N2) influenza virus.

    abstract：:The live cold-adapted (ca) A/AA/6/60 influenza vaccine is being commercially developed for worldwide use in children and is being used as a model for other live vaccines. Although it has been proven safe and immunogenic, the molecular basis of cold adaptation has never been determined. To identify sequence changes res...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.90.13.6032

    authors： Herlocher ML,Maassab HF,Webster RG

    更新日期：1993-07-01 00:00:00

  • Comparative organization of cattle chromosome 5 revealed by comparative mapping by annotation and sequence similarity and radiation hybrid mapping.

    abstract：:A whole genome cattle-hamster radiation hybrid cell panel was used to construct a map of 54 markers located on bovine chromosome 5 (BTA5). Of the 54 markers, 34 are microsatellites selected from the cattle linkage map and 20 are genes. Among the 20 mapped genes, 10 are new assignments that were made by using the compa...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.050007097

    authors： Ozawa A,Band MR,Larson JH,Donovan J,Green CA,Womack JE,Lewin HA

    更新日期：2000-04-11 00:00:00

  • The cation radicals of free base and zinc bacteriochlorin, bacteriochlorophyll, and bacteriopheophytin.

    abstract：:One-electron oxidation of zinc tetraphenylbacteriochlorin and its metal-free base yielded stable cation radicals. Electron spin resonance hyperfine splittings were assigned by selective deuterations. These results indicate that the protons of the saturated rings of the bacteriochlorins carry large spin densities, in a...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.71.3.994

    authors： Fajer J,Borg DC,Forman A,Felton RH,Dolphin D,Vegh L

    更新日期：1974-03-01 00:00:00

  • The nucleosome core particle remembers its position through DNA replication and RNA transcription.

    abstract：:Nucleosomes are the fundamental structural unit of chromatin. In addition to stabilizing the DNA polymer, nucleosomes are modified in ways that reflect and affect gene expression in their vicinity. It has long been assumed that nucleosomes can transmit memory of gene expression through their covalent posttranslational...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1911943116

    authors： Schlissel G,Rine J

    更新日期：2019-10-08 00:00:00

  • Leveraging scientific credibility about Arctic sea ice trends in a polarized political environment.

    abstract：:This work argues that, in a polarized environment, scientists can minimize the likelihood that the audience's biased processing will lead to rejection of their message if they not only eschew advocacy but also, convey that they are sharers of knowledge faithful to science's way of knowing and respectful of the audienc...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1320868111

    authors： Jamieson KH,Hardy BW

    更新日期：2014-09-16 00:00:00

  • Role of DNA mismatch repair and p53 in signaling induction of apoptosis by alkylating agents.

    abstract：:All cells are unavoidably exposed to chemicals that can alkylate DNA to form genotoxic damage. Among the various DNA lesions formed, O(6)-alkylguanine lesions can be highly cytotoxic, and we recently demonstrated that O(6)-methylguanine (O(6)MeG) and O(6)-chloroethylguanine (O(6)CEG) specifically initiate apoptosis in...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.96.19.10764

    authors： Hickman MJ,Samson LD

    更新日期：1999-09-14 00:00:00

  • Rescue of paclitaxel sensitivity by repression of Prohibitin1 in drug-resistant cancer cells.

    abstract：:Paclitaxel has emerged as a front line treatment for aggressive malignancies of the breast, lung, and ovary. Successful therapy of cancer is frequently undermined by the development of paclitaxel resistance. There is a growing need to find other therapeutic targets to facilitate treatment of drug-resistant cancers. Us...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0910649107

    authors： Patel N,Chatterjee SK,Vrbanac V,Chung I,Mu CJ,Olsen RR,Waghorne C,Zetter BR

    更新日期：2010-02-09 00:00:00

  • MDM2 inhibits p300-mediated p53 acetylation and activation by forming a ternary complex with the two proteins.

    abstract：:p300 acetylates and activates the tumor suppressor p53 after DNA damage. Here, we show that MDM2, a negative-feedback regulator of p53, inhibited p300-mediated p53 acetylation by complexing with these two proteins. First, we purified a p300-MDM2-p53 protein complex from HeLa nuclear extracts, which was inactive in p53...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.97.23.12547

    authors： Kobet E,Zeng X,Zhu Y,Keller D,Lu H

    更新日期：2000-11-07 00:00:00

  • Spatial games and the maintenance of cooperation.

    abstract：:The Prisoner's Dilemma (PD) is a widely employed metaphor for problems associated with the evolution of cooperative behavior. We have recently proposed an alternative approach to the PD, by exploring "spatial games" in which players--who are either pure cooperators, C, or pure defectors, D--interact with neighbors in ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.91.11.4877

    authors： Nowak MA,Bonhoeffer S,May RM

    更新日期：1994-05-24 00:00:00

  • Significant liquid-structure theory of viscosity and self-diffusion of the alkali metals.

    abstract：:Significant liquid-structure theory has been used to calculate the transport properties of the alkali metals. Unlike previous calculations, the volume and temperature dependence of the solid-like structure has been taken into consideration. Equations for the potential energy as a function of volume of the alkali metal...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.69.6.1342

    authors： Hsu CC,Eyring H

    更新日期：1972-06-01 00:00:00

  • Membrane-mobility agent-promoted fusion of erythrocytes: fusibility is correlated with attack by calcium-activated cytoplasmic proteases on membrane proteins.

    abstract：:Rat, but not human, erythrocytes undergo fusion promoted by the membrane-mobility agent 2-(2-methoxyethoxy)-ethyl cis-8-(2-octylcyclopropyl)octanoate (A2C). The difference in behavior is correlated with rat erythrocyte membrane protein degradation caused by Ca2+-activated proteases. The human erythrocyte is deficient ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.80.24.7542

    authors： Kosower NS,Glaser T,Kosower EM

    更新日期：1983-12-01 00:00:00

  • Revealing the structure of a catalytic combustion active-site ensemble combining uniform nanocrystal catalysts and theory insights.

    abstract：:Supported metal catalysts are extensively used in industrial and environmental applications. To improve their performance, it is crucial to identify the most active sites. This identification is, however, made challenging by the presence of a large number of potential surface structures that complicate such an assignm...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2002342117

    authors： Yang AC,Choksi T,Streibel V,Aljama H,Wrasman CJ,Roling LT,Goodman ED,Thomas D,Bare SR,Sánchez-Carrera RS,Schäfer A,Li Y,Abild-Pedersen F,Cargnello M

    更新日期：2020-06-30 00:00:00

  • Suppression of phase separation and giant enhancement of superconducting transition temperature in FeSe(1-x)Te(x) thin films.

    abstract：:We demonstrate the successful fabrication on CaF2 substrates of FeSe(1-x)Tex films with 0 ≤ x ≤ 1, including the region of 0.1 ≤ x ≤ 0.4, which is well known to be the "phase-separation region," via pulsed laser deposition that is a thermodynamically nonequilibrium method. In the resulting films, we observe a giant en...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1418994112

    authors： Imai Y,Sawada Y,Nabeshima F,Maeda A

    更新日期：2015-02-17 00:00:00

  • Patterned deposition of cells and proteins onto surfaces by using three-dimensional microfluidic systems.

    abstract：:Three-dimensional microfluidic systems were fabricated and used to pattern proteins and mammalian cells on a planar substrate. The three-dimensional topology of the microfluidic network in the stamp makes this technique a versatile one with which to pattern multiple types of proteins and cells in complex, discontinuou...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.040562297

    authors： Chiu DT,Jeon NL,Huang S,Kane RS,Wargo CJ,Choi IS,Ingber DE,Whitesides GM

    更新日期：2000-03-14 00:00:00

  • Neuritin: a gene induced by neural activity and neurotrophins that promotes neuritogenesis.

    abstract：:Neural activity and neurotrophins induce synaptic remodeling in part by altering gene expression. A cDNA encoding a glycosylphoshatidylinositol-anchored protein was identified by screening for hippocampal genes that are induced by neural activity. This molecule, named neuritin, is expressed in postmitotic-differentiat...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.94.6.2648

    authors： Naeve GS,Ramakrishnan M,Kramer R,Hevroni D,Citri Y,Theill LE

    更新日期：1997-03-18 00:00:00

  • Ha-ras oncogene expression directed by a milk protein gene promoter: tissue specificity, hormonal regulation, and tumor induction in transgenic mice.

    abstract：:The activated human Ha-ras oncogene was subjected to the control of the promoter region of the murine whey acidic protein (Wap) gene, which is expressed in mammary epithelial cells in response to lactogenic hormones. The Wap-ras gene was stably introduced into the mouse germ line of five transgenic mice (one male and ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.84.5.1299

    authors： Andres AC,Schönenberger CA,Groner B,Hennighausen L,LeMeur M,Gerlinger P

    更新日期：1987-03-01 00:00:00

  • Oldest known dinosaurian nesting site and reproductive biology of the Early Jurassic sauropodomorph Massospondylus.

    abstract：:The extensive Early Jurassic continental strata of southern Africa have yielded an exceptional record of dinosaurs that includes scores of partial to complete skeletons of the sauropodomorph Massospondylus, ranging from embryos to large adults. In 1976 an incomplete egg clutch including in ovo embryos of this dinosaur...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1109385109

    authors： Reisz RR,Evans DC,Roberts EM,Sues HD,Yates AM

    更新日期：2012-02-14 00:00:00

  • Supermassive black-hole growth over cosmic time: active galaxy demography, physics, and ecology from Chandra surveys.

    abstract：:Extragalactic X-ray surveys over the past decade have dramatically improved understanding of the majority populations of active galactic nuclei (AGNs) over most of the history of the universe. Here we briefly highlight some of the exciting discoveries about AGN demography, physics, and ecology, with a focus on results...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0914151107

    authors： Brandt WN,Alexander DM

    更新日期：2010-04-20 00:00:00

  • Generation and rescue of a murine model of platelet dysfunction: the Bernard-Soulier syndrome.

    abstract：:The human Bernard-Soulier syndrome is an autosomal recessive disorder of platelet dysfunction presenting with mild thrombocytopenia, circulating "giant" platelets and a bleeding phenotype. The bleeding in patients with the Bernard-Soulier syndrome is disproportionately more severe than suggested by the reduced platele...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.050582097

    authors： Ware J,Russell S,Ruggeri ZM

    更新日期：2000-03-14 00:00:00

  • Timing the impact of literacy on visual processing.

    abstract：:Learning to read requires the acquisition of an efficient visual procedure for quickly recognizing fine print. Thus, reading practice could induce a perceptual learning effect in early vision. Using functional magnetic resonance imaging (fMRI) in literate and illiterate adults, we previously demonstrated an impact of ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1417347111

    authors： Pegado F,Comerlato E,Ventura F,Jobert A,Nakamura K,Buiatti M,Ventura P,Dehaene-Lambertz G,Kolinsky R,Morais J,Braga LW,Cohen L,Dehaene S

    更新日期：2014-12-09 00:00:00

  • Reconstitution of tobacco mosaic virus rods occurs bidirectionally from an internal initiation region: demonstration by electron microscopic serology.

    abstract：:RNA of a common strain of tobacco mosaic virus was sequentially reconstituted first with protein of the same strain and then with protein of a tomato strain. The reconstituted particles were treated with specific antibodies against the two strain particles and were examined electron microscopically to locate the regio...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.74.5.1913

    authors： Otsuki Y,Takebe I,Ohno T,Fukuda M,Okada Y

    更新日期：1977-05-01 00:00:00

  • Cardiac allograft survival in mice treated with IL-2-PE40.

    abstract：:IL-2-PE40 is a chimeric protein composed of human interleukin 2 (IL-2) genetically fused to the amino terminus of a modified form of Pseudomonas exotoxin lacking its cell recognition domain. IL-2-PE40, which is extremely cytotoxic to IL-2 receptor-positive cells, was examined for its ability to prevent graft rejection...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.86.3.1008

    authors： Lorberboum-Galski H,Barrett LV,Kirkman RL,Ogata M,Willingham MC,FitzGerald DJ,Pastan I

    更新日期：1989-02-01 00:00:00

  • Defective T cell development and function in calcineurin A beta -deficient mice.

    abstract：:The calcium-dependent phosphatase calcineurin and its downstream transcriptional effector nuclear factor of activated T cells (NFAT) are important regulators of inducible gene expression in multiple cell types. In T cells, calcineurin-NFAT signaling represents a critical event for mediating cellular activation and the...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.152665399

    authors： Bueno OF,Brandt EB,Rothenberg ME,Molkentin JD

    更新日期：2002-07-09 00:00:00

  • Sequence intrinsic somatic mutation mechanisms contribute to affinity maturation of VRC01-class HIV-1 broadly neutralizing antibodies.

    abstract：:Variable regions of Ig chains provide the antigen recognition portion of B-cell receptors and derivative antibodies. Ig heavy-chain variable region exons are assembled developmentally from V, D, J gene segments. Each variable region contains three antigen-contacting complementarity-determining regions (CDRs), with CDR...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1709203114

    authors： Hwang JK,Wang C,Du Z,Meyers RM,Kepler TB,Neuberg D,Kwong PD,Mascola JR,Joyce MG,Bonsignori M,Haynes BF,Yeap LS,Alt FW

    更新日期：2017-08-08 00:00:00

  • Antitumor activity of mannan-binding protein in vivo as revealed by a virus expression system: mannan-binding proteindependent cell-mediated cytotoxicity.

    abstract：:Mannan-binding protein (MBP), a Ca2+-dependent mammalian lectin specific for mannose and N-acetylglucosamine, is an important serum component associated with innate immunity. MBP activates complement and functions as a direct opsonin on binding to mannooligosaccharide-bearing pathogens. We have found that MBP recogniz...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.96.2.371

    authors： Ma Y,Uemura K,Oka S,Kozutsumi Y,Kawasaki N,Kawasaki T

    更新日期：1999-01-19 00:00:00

  • Energies and kinetics of radical pairs involving bacteriochlorophyll and bacteriopheophytin in bacterial reaction centers.

    abstract：:Absorbance changes reflecting the formation of a transient radical-pair state, P(F), were measured in reaction centers from Rhodopseudomonas sphaeroides under conditions that blocked electron transfer to a later carrier (a quinone, Q). The temperature dependence of the absorbance changes suggests that P(F) is an equil...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.78.2.957

    authors： Shuvalov VA,Parson WW

    更新日期：1981-02-01 00:00:00

  • An antimicrobial peptide essential for bacterial survival in the nitrogen-fixing symbiosis.

    abstract：:In the nitrogen-fixing symbiosis between legume hosts and rhizobia, the bacteria are engulfed by a plant cell membrane to become intracellular organelles. In the model legume Medicago truncatula, internalization and differentiation of Sinorhizobium (also known as Ensifer) meliloti is a prerequisite for nitrogen fixati...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1500123112

    authors： Kim M,Chen Y,Xi J,Waters C,Chen R,Wang D

    更新日期：2015-12-08 00:00:00

  • Higher-order syzygies for the bracket algebra and for the ring of coordinates of the Grassmanian.

    abstract：:A Poincaré resolution is given for the supersymmetric ring of brackets over a signed alphabet. As a consequence, a resolution is found for the ring of coordinates of the Grassmanian variety in projective space over any infinite field. ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.88.18.8087

    authors： Anick D,Rota GC

    更新日期：1991-09-15 00:00:00

  • Energetics of the exchangeable quinone, QB, in Photosystem II.

    abstract：:Photosystem II (PSII), the light-driven water/plastoquinone photooxidoreductase, is of central importance in the planetary energy cycle. The product of the reaction, plastohydroquinone (PQH2), is released into the membrane from the QB site, where it is formed. A plastoquinone (PQ) from the membrane pool then binds int...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1910675116

    authors： De Causmaecker S,Douglass JS,Fantuzzi A,Nitschke W,Rutherford AW

    更新日期：2019-09-24 00:00:00

  • Plant homologs of the Plasmodium falciparum chloroquine-resistance transporter, PfCRT, are required for glutathione homeostasis and stress responses.

    abstract：:In Arabidopsis thaliana, biosynthesis of the essential thiol antioxidant, glutathione (GSH), is plastid-regulated, but many GSH functions, including heavy metal detoxification and plant defense activation, depend on cytosolic GSH. This finding suggests that plastid and cytosol thiol pools are closely integrated and we...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0913689107

    authors： Maughan SC,Pasternak M,Cairns N,Kiddle G,Brach T,Jarvis R,Haas F,Nieuwland J,Lim B,Müller C,Salcedo-Sora E,Kruse C,Orsel M,Hell R,Miller AJ,Bray P,Foyer CH,Murray JA,Meyer AJ,Cobbett CS

    更新日期：2010-02-02 00:00:00