Defective T cell development and function in calcineurin A beta -deficient mice.

abstract：:We previously reported that laminar flow activates peroxisome proliferator-activated receptor gamma (PPARgamma) in vascular endothelial cells in a ligand-dependent manner that involves phospholipase A2 and cytochrome P450 epoxygenases. In this study, we investigated whether epoxyeicosatrienoic acids (EETs), the cataly...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu Y,Zhang Y,Schmelzer K,Lee TS,Fang X,Zhu Y,Spector AA,Gill S,Morisseau C,Hammock BD,Shyy JY

更新日期：2005-11-15 00:00:00

abstract：:Using single-cell sequence analysis, we discovered that a high proportion of cells in tissues as diverse as buccal epithelium and heart muscle contain high proportions of clonal mutant mtDNA expanded from single initial mutant mtDNA molecules. We demonstrate that intracellular clonal expansion of somatic point mutatio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nekhaeva E,Bodyak ND,Kraytsberg Y,McGrath SB,Van Orsouw NJ,Pluzhnikov A,Wei JY,Vijg J,Khrapko K

更新日期：2002-04-16 00:00:00

abstract：:We have isolated a protein from Saccharomyces cerevisiae that binds specifically to a nucleotide sequence associated with the autonomously replicating sequence (ARS) ARS120, located in the telomeric region of a yeast chromosome. "Footprinting" analysis revealed that a 26-base-pair DNA sequence, 5'-CAAGTGCCGTGCATAATGAT...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eisenberg S,Civalier C,Tye BK

更新日期：1988-02-01 00:00:00

abstract：:Prokaryotic genomes are substantially diverse, even when from closely related species, with the resulting phenotypic diversity representing a repertoire of adaptations to specific constraints. Within the microbial population, genome content may not be fixed, as changing selective forces favor particular phenotypes; ho...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aras RA,Kang J,Tschumi AI,Harasaki Y,Blaser MJ

更新日期：2003-11-11 00:00:00

abstract：:We reported previously that Go-deficient mice develop severe neurological defects that include hyperalgesia, a generalized tremor, lack of coordination, and a turning syndrome somewhat reminiscent of unilateral lesions of the dopaminergic nigro-striatal pathway. By using frozen coronal sections of serially sectioned b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jiang M,Spicher K,Boulay G,Wang Y,Birnbaumer L

更新日期：2001-03-13 00:00:00

abstract：:We compared folding properties of designed protein Top7 and natural protein S6 by using coarse-grained chain models with a mainly native-centric construct that accounted also for nonnative hydrophobic interactions and desolvation barriers. Top7 and S6 have similar secondary structure elements and are approximately equ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang Z,Chan HS

更新日期：2010-02-16 00:00:00

abstract：:The Sleeping Beauty (SB) transposon system can integrate foreign sequences of DNA in the genome of mouse somatic cells eliciting long-term expression in vivo. This technology holds great promise for human gene therapy as a nonviral technology to deliver therapeutic genes. SB also provides a means to study the effects ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carlson CM,Frandsen JL,Kirchhof N,McIvor RS,Largaespada DA

更新日期：2005-11-22 00:00:00

abstract：:Periodic fluctuations in past biodiversity, speciation, and extinction have been proposed, with extremely long periods ranging from 26 to 62 million years, although forcing mechanisms remain speculative. In contrast, well-understood periodic Milankovitch climate forcing represents a viable driver for macroevolutionary...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Crampton JS,Meyers SR,Cooper RA,Sadler PM,Foote M,Harte D

更新日期：2018-05-29 00:00:00

abstract：:Aspirin-exacerbated respiratory disease (AERD) is characterized by asthma, tissue eosinophilia, overproduction of cysteinyl leukotrienes (cysLTs), and respiratory reactions to nonselective cyclooxygenase (COX) inhibitors. Ex vivo studies suggest that functional abnormalities of the COX-2/microsomal prostaglandin (PG)E...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu T,Laidlaw TM,Katz HR,Boyce JA

更新日期：2013-10-15 00:00:00

abstract：:Ribosomal RNA (rRNA) genes are down-regulated during osteogenesis, myogenesis, and adipogenesis, necessitating a mechanistic understanding of interrelationships between growth control and phenotype commitment. Here, we show that cell fate-determining factors [MyoD, myogenin (Mgn), Runx2, C/EBPbeta] occupy rDNA loci an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ali SA,Zaidi SK,Dacwag CS,Salma N,Young DW,Shakoori AR,Montecino MA,Lian JB,van Wijnen AJ,Imbalzano AN,Stein GS,Stein JL

更新日期：2008-05-06 00:00:00

abstract：:The accuracy of tail-anchored (TA) protein targeting to the endoplasmic reticulum (ER) depends on the Guided Entry of Tail-Anchored (Get) protein targeting machinery. The fate of TA proteins that become inappropriately inserted into other organelles, such as mitochondria, is unknown. Here, we identify Msp1, a conserve...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Okreglak V,Walter P

更新日期：2014-06-03 00:00:00

abstract：:The heterodimeric Elongin BC complex has been shown to interact in vitro and in cells with a conserved BC-box motif found in an increasing number of proteins including RNA polymerase II elongation factor Elongin A, suppressor of cytokine signaling (SOCS)-box proteins, and the von Hippel-Lindau tumor suppressor protein...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brower CS,Sato S,Tomomori-Sato C,Kamura T,Pause A,Stearman R,Klausner RD,Malik S,Lane WS,Sorokina I,Roeder RG,Conaway JW,Conaway RC

更新日期：2002-08-06 00:00:00

abstract：:Capsaicin is a unique alkaloid of the plant kingdom restricted to the genus Capsicum. Capsaicin is the pungency factor, a bioactive molecule of food and of medicinal importance. Capsaicin is useful as a counterirritant, antiarthritic, analgesic, antioxidant, and anticancer agent. Capsaicin biosynthesis involves conden...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Prasad BC,Kumar V,Gururaj HB,Parimalan R,Giridhar P,Ravishankar GA

更新日期：2006-09-05 00:00:00

abstract：:Dietary diversity often varies inversely with prey resource abundance. This pattern, although typically measured at the population level, is usually assumed to also characterize the behavior of individual animals within the population. However, the pattern might also be produced by changes in the degree of variation a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tinker MT,Bentall G,Estes JA

更新日期：2008-01-15 00:00:00

abstract：:Although current demands for therapeutic mAbs are growing quickly, production methods to date, including in vitro mammalian tissue culture and transgenic animals, provide only limited quantities at high cost. Several tumor-associated antigens in tumor cells have been identified as targets for therapeutic mAbs. Here we...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brodzik R,Glogowska M,Bandurska K,Okulicz M,Deka D,Ko K,van der Linden J,Leusen JH,Pogrebnyak N,Golovkin M,Steplewski Z,Koprowski H

更新日期：2006-06-06 00:00:00

abstract：:The prgB gene encodes the surface protein, Asc10, which mediates cell aggregation, resulting in high-frequency conjugative transfer of the pheromone-inducible tetracycline-resistance plasmid pCF10 in Enterococcus faecalis. Messenger RNA analysis by Northern blot hybridization and primer extension indicates that prgB t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chung JW,Dunny GM

更新日期：1992-10-01 00:00:00

abstract：:The gluconate (gnt) operon of Bacillus subtilis consists of four gnt genes; the second and third genes code for gluconate kinase (gluconokinase, EC 2.7.1.12) and gluconate permease, respectively. A fragment carrying the promoter of this operon (gnt promoter) and the first gene (gntR) was subcloned into a promoter prob...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fujita Y,Fujita T

更新日期：1987-07-01 00:00:00

abstract：:We have used expression cloning in Xenopus oocytes to isolate two different cDNAs encoding functional N-methyl-D-aspartate (NMDA) receptor subunits. The two receptors (NMDA-R1A and -R1B) display different pharmacologic properties as a consequence of alternative exon addition within the putative ligand-binding domain. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nakanishi N,Axel R,Shneider NA

更新日期：1992-09-15 00:00:00

abstract：:Visualizing macromolecular complexes by single-particle electron microscopy (EM) entails stringent biochemical purification, specimen preparation, low-dose imaging, and 3D image reconstruction. Here, we introduce the "monolayer purification" method, which employs nickel-nitrilotriacetic acid (Ni-NTA) functionalized li...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kelly DF,Dukovski D,Walz T

更新日期：2008-03-25 00:00:00

abstract：:We have isolated and analyzed a cDNA from the central nervous system of the mollusc Lymnaea stagnalis encoding a putative receptor, which might be a natural hybrid between two different classes of receptor proteins. Preceded by a signal peptide, two types of repeated sequences are present in the N-terminal part of the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tensen CP,Van Kesteren ER,Planta RJ,Cox KJ,Burke JF,van Heerikhuizen H,Vreugdenhil E

更新日期：1994-05-24 00:00:00

abstract：:A genetic analysis is necessary to gain a greater understanding of the complex developmental processes in mammals. Toward this end, an insertional transgenic mouse mutant has been isolated that results in abnormal germ-cell development. This recessive mutation manifests as infertility in both males and females and is ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pellas TC,Ramachandran B,Duncan M,Pan SS,Marone M,Chada K

更新日期：1991-10-01 00:00:00

abstract：:Transcription factor TFIID consists of TATA binding protein (TBP) and at least eight TBP-associated factors (TAFs). As TAFs are required for activated but not basal transcription, we have proposed that TAFs act as coactivators to mediate signals between activators and the basal transcription machinery. Here we report ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Klemm RD,Goodrich JA,Zhou S,Tjian R

更新日期：1995-06-20 00:00:00

abstract：:We determined the nucleotide sequence of about 1 kilobase of DNA 3' to the 5' long terminal repeat of three noninfectious ad one infectious proviral DNA clones of spleen necrosis virus, an avian retrovirus, to determine if the types of nucleic acid changes involved in retrovirus mutation shed light on special features...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： O'Rear JJ,Temin HM

更新日期：1982-02-01 00:00:00

abstract：:Aluminum (Al) is the most abundant metal in the Earth's crust, but its trivalent ionic form is highly toxic to all organisms at low concentrations. How Al enters cells has not been elucidated in any organisms. Herein, we report a transporter, Nrat1 (Nramp aluminum transporter 1), specific for trivalent Al ion in rice....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xia J,Yamaji N,Kasai T,Ma JF

更新日期：2010-10-26 00:00:00

abstract：:The importance of proper ion channel trafficking is underpinned by a number of channel-linked genetic diseases whose defect is associated with failure to reach the cell surface. Conceptually, it is reasonable to suggest that the function of ion channels depends critically on the precise subcellular localization and th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lu L,Timofeyev V,Li N,Rafizadeh S,Singapuri A,Harris TR,Chiamvimonvat N

更新日期：2009-10-27 00:00:00

abstract：:Calmodulin, a wide-spread eukaryotic Ca2+-binding protein, was phosphorylated at its tyrosine residues in Rous sarcoma virus (RSV)-transformed chicken and rat cells but not in normal chicken embryo fibroblasts. In contrast, serine and threonine phosphorylation of calmodulin was found to occur in both normal and virus-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fukami Y,Nakamura T,Nakayama A,Kanehisa T

更新日期：1986-06-01 00:00:00

abstract：:Opitz syndrome (OS) is a genetically heterogeneous disorder characterized by defects of the ventral midline, including hypertelorism, cleft lip and palate, heart defects, and mental retardation. We recently identified the gene responsible for X-linked OS. The ubiquitously expressed gene product, MID1, is a member of t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schweiger S,Foerster J,Lehmann T,Suckow V,Muller YA,Walter G,Davies T,Porter H,van Bokhoven H,Lunt PW,Traub P,Ropers HH

更新日期：1999-03-16 00:00:00

abstract：:Life history events, such as traumatic stress, illness, or starvation, can influence us through molecular changes that are recorded in a pattern of characteristic chromatin modifications. These modifications are often associated with adaptive adjustments in gene expression that can persist throughout the lifetime of t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Demoinet E,Li S,Roy R

更新日期：2017-03-28 00:00:00

abstract：:The phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway was recently shown to negatively regulate LPS-induced acute inflammatory responses. We previously observed that the metabolic thiol antioxidant alpha-lipoic acid (LA) inhibits LPS-induced expression of cellular adhesion molecules and adheren...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang WJ,Wei H,Hagen T,Frei B

更新日期：2007-03-06 00:00:00

abstract：:In 1989, populations of North American gypsy moth, Lymantria dispar, in seven contiguous northeastern states were severely reduced by a fungal pathogen. Based on morphology, development, and pathology, this organism appeared to be Entomophaga maimaiga. We have now used allozyme and restriction fragment length polymorp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hajek AE,Humber RA,Elkinton JS,May B,Walsh SR,Silver JC

更新日期：1990-09-01 00:00:00