Abstract:
:The heterodimeric Elongin BC complex has been shown to interact in vitro and in cells with a conserved BC-box motif found in an increasing number of proteins including RNA polymerase II elongation factor Elongin A, suppressor of cytokine signaling (SOCS)-box proteins, and the von Hippel-Lindau tumor suppressor protein. Recently, the Elongin BC complex was found to function as an adaptor that links these BC-box proteins to a module composed of Cullin family members Cul2 or Cul5 and RING-H2 finger protein Rbx1 to reconstitute a family of E3 ubiquitin ligases that activate ubiquitylation by the E2 ubiquitin-conjugating enzyme Ubc5. As part of our effort to understand the functions of Elongin BC-based ubiquitin ligases, we exploited a modified yeast two-hybrid screen to identify a mammalian BC-box protein similar in sequence to Saccharomyces cerevisiae Mediator subunit Med8p. In this report we demonstrate (i) that mammalian MED8 is a subunit of the mammalian Mediator complex and (ii) that MED8 can assemble with Elongins B and C, Cul2, and Rbx1 to reconstitute a ubiquitin ligase. Taken together, our findings are consistent with the model that MED8 could function to recruit ubiquitin ligase activity directly to the RNA polymerase II transcriptional machinery.
journal_name
Proc Natl Acad Sci U S Aauthors
Brower CS,Sato S,Tomomori-Sato C,Kamura T,Pause A,Stearman R,Klausner RD,Malik S,Lane WS,Sorokina I,Roeder RG,Conaway JW,Conaway RCdoi
10.1073/pnas.162424199keywords:
subject
Has Abstractpub_date
2002-08-06 00:00:00pages
10353-8issue
16eissn
0027-8424issn
1091-6490pii
162424199journal_volume
99pub_type
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