Abstract:
ETHNOPHARMACOLOGICAL RELEVANCE:The blister beetle, also known as Mylabris cichorii, is not only widely used in clinical applications in Chinese anticancer medicine, but is also one of the main ingredients in a variety of traditional Chinese medicinal preparations with anticancer activity. However, the strong toxicity exhibited by this beetle species limits its clinical applicability, with the photosynthetic bacteria featuring a strong biological conversion function. Therefore, in this study we explore the use of photosynthetic bacteria for bioconversion of the blister beetle in order to reduce the toxicity and in effort to enhance the overall antitumor effect. METHODS AND RESULTS:In the first set of experiments, we utilized an orthogonal experimental design to optimize the culture medium of photosynthetic bacteria. Concurrently, the growth curve of photosynthetic bacteria was used to determine the inoculation amount of the photosynthetic bacteria and the safe concentration of cantharis. Through antitumor activity experiments conducted in vitro we found that the inhibition rate increased through cantharis by PSB biotransformation of HepG2, A549 and BEL-7406 cells. Furthermore, through acute toxicity tests in mice it was found that the blister beetle water extraction liquid exhibits a LD50 value of 1383mg/kg, while the blister beetle transformation liquid exhibits a LD50 value of 206mg/kg. The LD50 value of the blister beetle water extract was found to be 6.7 times higher than the transformation liquid, thus demonstrating that the toxicity of cantharis by PSB biotransformation may be decreased. More strikingly, decreased toxicity was observed in the mouse liver and in pathological sections of the kidneys after transformation. CONCLUSIONS:In this paper we demonstrate for the first time that PSB bioconversion of the blister beetle is able to reduce the toxicity of a common method used in anticancer treatments as part of the principles in traditional Chinese medicine and may therefore improve antitumor activity in vitro.
journal_name
J Ethnopharmacoljournal_title
Journal of ethnopharmacologyauthors
Yu M,Zhao Ydoi
10.1016/j.jep.2016.03.058subject
Has Abstractpub_date
2016-06-20 00:00:00pages
151-158eissn
0378-8741issn
1872-7573pii
S0378-8741(16)30172-6journal_volume
186pub_type
杂志文章abstract::Since ages Mallotus peltatus (Geist) Muell. Arg. var acuminatus (Euphorbiaceae) leaf and stem bark is used in folk medicine to cure intestinal ailments and skin infections. In several intestinal ailments, localized inflammation is of common occurrence and hence we have evaluated the antimicrobial as well as anti-infla...
journal_title:Journal of ethnopharmacology
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journal_title:Journal of ethnopharmacology
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journal_title:Journal of ethnopharmacology
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journal_title:Journal of ethnopharmacology
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journal_title:Journal of ethnopharmacology
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journal_title:Journal of ethnopharmacology
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journal_title:Journal of ethnopharmacology
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journal_title:Journal of ethnopharmacology
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journal_title:Journal of ethnopharmacology
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abstract::Fruiting bodies of Phellinus linteus were extracted with 70% ethanol at room temperature. The Phellinus linteus extract (PL) showed strong anti-angiogenic activity, which was detected using the chick embryo chorioallantoic membrane (CAM) assay. The in vitro antioxidant activities of PL were evaluated using two differe...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
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更新日期:2003-09-01 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章,评审
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更新日期:2016-04-02 00:00:00
abstract::Oxymatrine is one of the alkaloids extracted from Chinese herb Sophora japonica (Sophora flavescens Ait.) with activities of anti-inflammation, inhibiting immune reaction, antivirus, protecting hepatocytes and antihepatic fibrosis. However, the effect of oxymatrine on acute lung injury (ALI) has not been known yet. In...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
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更新日期:2005-04-08 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2015.09.005
更新日期:2015-12-04 00:00:00
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journal_title:Journal of ethnopharmacology
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更新日期:2016-03-02 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
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journal_title:Journal of ethnopharmacology
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journal_title:Journal of ethnopharmacology
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journal_title:Journal of ethnopharmacology
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journal_title:Journal of ethnopharmacology
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2013.10.062
更新日期:2014-01-01 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
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更新日期:1995-07-07 00:00:00
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journal_title:Journal of ethnopharmacology
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更新日期:2018-05-10 00:00:00
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journal_title:Journal of ethnopharmacology
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journal_title:Journal of ethnopharmacology
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
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更新日期:2012-01-31 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
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更新日期:2019-06-12 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Wan-Sheng-Hua-Feng-Dan (WSHFD) is a traditional Chinese medicine used for the treatment of neurological disorders. Cinnabar (HgS) and realgar (As(4)S(4)) are included in WSHFD. Are they remedies or poisons? AIM OF STUDY:To investigate the role of cinnabar and realgar in the protective ef...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2011.12.026
更新日期:2012-02-15 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Cimicifuga foetida L., a traditional Chinese medicine, has been used as an anti-inflammatory, antipyretic and analgesic remedy. The primary active constituents are believed to be present in the triterpene glycoside fraction. MATERIALS AND METHODS:To develop an LC-MS/MS assay for four maj...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2012.06.031
更新日期:2012-08-30 00:00:00