Abstract:
:Pathogen attachment to host cells is a key process during infection, and inhibition of pathogen adhesion is a promising approach to the prevention of infectious disease. We have previously shown that multivalent adhesion molecules (MAMs) are abundant in both pathogenic and commensal bacterial species, mediate early attachment to host cells, and can contribute to virulence. Here, we investigated the efficacy of an engineered bacterium expressing a commensal MAM on its surface in preventing pathogen attachment and pathogen-mediated cytotoxicity in a tissue culture infection model. We were able to dissect the individual contributions of adhesion and interspecific antagonism on the overall outcome of infection for a range of different pathogens by comparison with the results obtained with a fully synthetic adhesion inhibitor. We found that the potential of the engineered bacterium to outcompete the pathogen is not always solely dependent on its ability to hinder host attachment but, depending on the pathogenic species, may also include elements of interspecific antagonism, such as competition for nutrients and its ability to cause a loss of fitness due to production of antimicrobial factors.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Al-Saedi F,Stones DH,Vaz DP,Krachler AMdoi
10.1128/IAI.00020-16subject
Has Abstractpub_date
2016-05-24 00:00:00pages
1704-1711issue
6eissn
0019-9567issn
1098-5522pii
IAI.00020-16journal_volume
84pub_type
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