Abstract：
:Cochlear hair cell loss results in the secondary loss of spiral ganglion cells (SGCs). The death of these SGCs is due to apoptosis. The E2F1-cyclin dependent kinase 1 (CDK1) pathway is believed to represent an important mechanism of neuronal cell death. However, the role of this pathway in spiral ganglion neuronal apoptosis has not yet been reported. In this study, we deafened guinea pigs with a subcutaneous injection of kanamycin followed by an intravenous infusion of furosemide and then assayed the expression levels of cleaved caspase-3, E2F1, CDK1 and cleaved caspase-9 during the induced SGC apoptosis. Our results revealed that co-administration of kanamycin and furosemide rapidly induced hair cell loss in the guinea pigs and then resulted in a progressive loss of SGCs. Expression levels of E2F1 and CDK1 were obviously up-regulated at 1 and 3 days after deafening. Cleaved caspase-9 also increased robustly 1 or 2 weeks after the deafening procedure. The up-regulation of E2F1, CDK1 and cleaved caspase-9 was significantly attenuated by the systemic injection of CR8 (1mg/kg/day, intraperitoneally) starting at 5min after deafening. These findings indicate that the activation of the E2F1-CDK1 pathway and cell cycle re-entry contributes to the apoptosis of SGCs and that the selective inhibition of this signaling cascade may represent an attractive therapeutic strategy. CR8 has the potential to protect SGCs from apoptosis.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Liu YY,Wang GP,Peng Z,Guo JY,Wu Q,Xie J,Gong SSdoi
10.1016/j.neulet.2016.02.034subject
Has Abstractpub_date
2016-03-23 00:00:00pages
247-53eissn
0304-3940issn
1872-7972pii
S0304-3940(16)30100-8journal_volume
617pub_type
杂志文章abstract：:The role of nitric oxide in cerebral insults remains controversial. While numerous studies have used models of ischaemia and hypoxia, few have examined nitric oxide in the kainate model of excitotoxicity. Kainate (10 mg/kg) was administered to rats via the intraperitoneal (i.p.) route to induce submaximal damage to th...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(98)00372-3
更新日期：1998-06-19 00:00:00
abstract：:Glucose metabolism and tricarboxylic acid cycle (TCA) kinetics have been shown to decline in brain with age in various animal species. This study examined TCA cycle kinetics and age in Brown-Norway Fischer 344 rats. Using [1-(13)C]glucose infused over 10, 30, 60 or 100 min, and following the label through the TCA cycl...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(00)00825-9
更新日期：2000-03-10 00:00:00
abstract：:Deep brain stimulation (DBS) is associated with substantial improvements in motor symptoms of PD. Emerging evidence has suggested that nonlinear measures of complexity may provide greater insight into the efficacy of anti-PD treatments. This study investigated sample entropy and complexity index values in individuals ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2018.05.010
更新日期：2020-05-29 00:00:00
abstract：:In the neuritic plaques of Alzheimer's disease, abnormal neuritic processes cluster around a core of beta-amyloid protein. Previous data have shown that beta 1-28, a peptide homologous to the first 28 amino acid residues of beta-amyloid protein, enhanced survival without affecting neuritic extension or branching in cu...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(90)90867-9
更新日期：1990-03-14 00:00:00
abstract：:Transmeatal cochlear laser (TCL) treatment has recently been proposed as a therapeutic procedure for cochlear dysfunction such as chronic cochlear tinnitus or sensorineural hearing loss. The aim of this study was to investigate whether TLC has any influence on the central nervous system using functional MRI with healt...
journal_title：Neuroscience letters
pub_type： 临床试验,杂志文章,随机对照试验
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(02)00003-4
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2004.01.030
更新日期：2004-04-22 00:00:00
abstract：:Most single fibers of the cochlear nerve (CN) in 22 cats exhibited effects of mechanical interaction in one cochlea between two sounds applied binaurally, similarly to results in two cats in which the contralateral CN was transected. In 11 of 189 fibers, the spontaneous and/or the sound-evoked activity was suppressed ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(80)90299-2
更新日期：1980-07-01 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(94)90607-6
更新日期：1994-04-25 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(01)02195-4
更新日期：2001-10-12 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2007.11.044
更新日期：2008-02-06 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(99)00769-7
更新日期：1999-11-19 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(94)91064-2
更新日期：1994-07-04 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2017.11.047
更新日期：2018-02-05 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2012.03.069
更新日期：2012-05-10 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(95)11445-3
更新日期：1995-04-07 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(98)00149-9
更新日期：1998-03-20 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2005.10.074
更新日期：2006-02-27 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(97)00664-2
更新日期：1997-10-03 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(89)90792-1
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2007.05.049
更新日期：2007-07-05 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2004.01.032
更新日期：2004-04-01 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(86)90449-0
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journal_title：Neuroscience letters
pub_type： 杂志文章
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更新日期：1986-12-23 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2020.135602
更新日期：2021-01-06 00:00:00
abstract：:The purpose of this study was to examine the effects of intraplantar dexmedetomidine to relieve neuropathic pain and determine the role of peripheral α2-adrenoceptors. Neuropathic pain was induced by ligating the L5 and L6 spinal nerves in male Sprague-Dawley rats, and mechanical allodynia was assessed using von Frey ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2013.10.002
更新日期：2013-12-17 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(93)90489-8
更新日期：1993-04-02 00:00:00
abstract：:The longest central nervous system (CNS) specific isoform of the major myelin-associated inhibitor of neurite growth, Nogo-A, has previously been shown to be expressed largely in oligodendrocytes. Using an antibody raised against a recombinant fusion protein comprising amino acids 223-399 of Nogo-A, we show in this re...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(02)00528-1
更新日期：2002-08-16 00:00:00
