Abstract：
:The present study was designed to determine the relationships between the performance of ethanol precipitation and seven process parameters in the ethanol precipitation process of Re Du Ning Injections, including concentrate density, concentrate temperature, ethanol content, flow rate and stir rate in the addition of ethanol, precipitation time, and precipitation temperature. Under the experimental and simulated production conditions, a series of precipitated resultants were prepared by changing these variables one by one, and then examined by HPLC fingerprint analyses. Different from the traditional evaluation model based on single or a few constituents, the fingerprint data of every parameter fluctuation test was processed with Principal Component Analysis (PCA) to comprehensively assess the performance of ethanol precipitation. Our results showed that concentrate density, ethanol content, and precipitation time were the most important parameters that influence the recovery of active compounds in precipitation resultants. The present study would provide some reference for pharmaceutical scientists engaged in research on pharmaceutical process optimization and help pharmaceutical enterprises adapt a scientific and reasonable cost-effective approach to ensure the batch-to-batch quality consistency of the final products.
journal_name
Chin J Nat Medjournal_title
Chinese journal of natural medicinesauthors
Sun LQ,Wang SY,Li YJ,Wang YX,Wang ZZ,Huang WZ,Wang YS,Bi YA,Ding G,Xiao Wdoi
10.3724/SP.J.1009.2015.00073subject
Has Abstractpub_date
2016-01-01 00:00:00pages
73-80issue
1eissn
2095-6975issn
1875-5364pii
S1875-5364(16)30012-7journal_volume
14pub_type
杂志文章abstract：:Atherosclerosis (AS) is a chronic inflammatory disease, the main causes of which include abnormal lipid metabolism, endothelial injury, physical and chemical injury, hemodynamic injury, genetic factors and so on. These causes can lead to inflammatory injury of blood vessels and local dysfunction. Bunao-Fuyuan decoctio...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(21)60004-3
更新日期：2021-01-01 00:00:00
abstract：AIM:The compound B19 (C21H22O5) is a newly synthesized, mono-carbonyl analog of curcumin that has exhibited potential antitumor effects. This present study was performed to identify the anti-angiogenic activity of this compound. METHODS AND RESULTS:B19 inhibited migration and tube formation of human umbilical vein end...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(14)60002-9
更新日期：2014-01-01 00:00:00
abstract：AIM:To study the chemical constituents of the fruits of Illicium henryi. METHOD:Chromatographic separations on silica gel, Sephadex LH-20 gel and MCI gel were used to isolate the compounds. The structures were elucidated based on extensive spectroscopic data analyses. RESULTS:Seven compounds were obtained and their s...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(14)60074-1
更新日期：2014-06-01 00:00:00
abstract：:To investigate the non-alkaloidal chemical constituents of the stems and leaves of Melodinus suaveolens and their antibacterial activities. Compounds were isolated and purified by repeated silica gel, Sephadex LH-20, RP18, and preparative HPLC. Their structures were elucidated by comparison with published spectroscopi...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(15)30020-0
更新日期：2015-04-01 00:00:00
abstract：:Sophorae Flavescentis Radix (Sophora flavescens Ait., SFR) and Sophorae Tonkinensis Radix et Rhizoma (S. tonkinensis Gapnep., STR) are two commonly used traditional Chinese medicines from Sophora (Leguminosae) plants, which are believed to possess similar bioactive components with entirely different clinical applicati...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(18)30137-7
更新日期：2018-12-01 00:00:00
abstract：:Panax notoginseng saponins (PNS) are the major components of Panax notoginseng, with multiple pharmacological activities but poor oral bioavailability. PNS could be metabolized by gut microbiota in vitro, while the exact role of gut microbiota of PNS metabolism in vivo remains poorly understood. In this study, pseudo ...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(19)30026-3
更新日期：2019-03-01 00:00:00
abstract：:Current study systematically investigated the interaction of two alkaloids, anisodine and monocrotaline, with organic cation transporter OCT1, 2, 3, MATE1 and MATE2-K by using in vitro stably transfected HEK293 cells. Both anisodine and monocrotaline inhibited the OCTs and MATE transporters. The lowest IC50 was 12.9 µ...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(19)30070-6
更新日期：2019-07-01 00:00:00
abstract：:The saponin ginsenoside Rk1 is a major compound isolated from ginseng. Ginsenoside Rk1 has been reported to have anti-inflammatory and anti-tumor properties and to be involved in the regulation of metabolism. However, the effect and mechanism of anti-inflammatory action of ginsenoside Rk1 has not been fully clarified....
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(17)30106-1
更新日期：2017-10-01 00:00:00
abstract：:Isochlorogenic acid A (ICQA), which has anti-inflammatory, hepatoprotective, and antiviral properties, is commonly presented in fruits, vegetables, coffee, plant-based food products, and herbal medicines. These herbal medicines are usually used in combination with other medicines in the clinic. However, little is know...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(16)30103-0
更新日期：2016-11-01 00:00:00
abstract：:This study engineered β-carotene ketolase CrtW and β-carotene hydroxylase CrtZ to improve biosynthesis of astaxanthin in Escherichia coli. Firstly, crtW was randomly mutated to increase CrtW activities on conversion from β-carotene to astaxanthin. A crtW* mutant with A6T, T105A and L239M mutations has improved 5.35-fo...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(20)60005-X
更新日期：2020-09-01 00:00:00
abstract：:Traditional Chinese medicines (TCMs) are in great demand all over the world, especially in the developing world, for primary health care due to their superior merits such as low cost, minimal side effects, better cultural acceptability, and compatibility with humans. However, Chinese medicines consist of several herbs...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章,评审
doi：10.1016/S1875-5364(13)60069-2
更新日期：2013-11-01 00:00:00
abstract：:Inflammation is recently recognized as one of the hallmarks of human cancer. Chronic inflammatory response plays a critical role in cancer development, progression, metastasis, and resistance to chemotherapy. Conversely, the oncogenic aberrations also generate an inflammatory microenvironment, enabling the development...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章,评审
doi：10.1016/S1875-5364(17)30062-6
更新日期：2017-06-01 00:00:00
abstract：AIM:To study the chemical constituents of the aerial parts of Cirsium setosum (Willd.) MB.. METHODS:The chemical constituents were isolated and purified by various chromatographic techniques. Their structures were determined on the basis of physical properties and spectroscopic data. RESULTS:A new megastigmane glycos...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(13)60097-7
更新日期：2013-09-01 00:00:00
abstract：:Penicildiones A-D (1-4), four new steroids derivatives together with three known compounds including 16α-methylpregna-17α,19-dihydroxy-(9,11)-epoxy-4-ene-3,18-dione-20-acetoxy (5), stachybotrylactone B (6) and stachybotrin (7) were isolated from the soft coral-derived fungus Penicillium sp. SCSIO41201, cultured in the...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(20)30030-3
更新日期：2020-04-01 00:00:00
abstract：:Three new phenazine-type compounds, named phenazines SA-SC (1-3), together with four new natural products (4-7), were isolated from the fermentation broth of an earwig-associated Streptomyces sp. NA04227. The structures of these compounds were determined by extensive analyses of NMR, high resolution mass spectroscopic...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(19)30055-X
更新日期：2019-06-01 00:00:00
abstract：:Naodesheng (NDS) formula, which consists of Rhizoma Chuanxiong, Lobed Kudzuvine, Carthamus tinctorius, Radix Notoginseng, and Crataegus pinnatifida, is widely applied for the treatment of cardio/cerebrovascular ischemic diseases, ischemic stroke, and sequelae of cerebral hemorrhage, etc. At present, the studies on NDS...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(18)30029-3
更新日期：2018-01-01 00:00:00
abstract：:Previous studies have indicated that the Ilex genus exhibits antioxidant, neuroprotective, hepatoprotective, and anti-inflammatory activities. However, the pharmacologic action and mechanisms of Ilex cornuta against cardiac diseases have not yet been explored. The present study was designed to investigate the antioxid...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(17)30025-0
更新日期：2017-02-01 00:00:00
abstract：:Diosgenin, a well-known steroid sapogenin derived from plants, has been used as a starting material for production of steroidal hormones. The present review will summarize published literature concerning pharmacological potential of diosgenin, and the underlying mechanisms of actions. Diosgenin has shown a vast range ...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章,评审
doi：10.1016/S1875-5364(15)30053-4
更新日期：2015-08-01 00:00:00
abstract：:Tripolinolate A (TLA) is recently identified as a new compound from a halophyte plant Tripolium vulgare and has been shown to have significant in vitro activity against the proliferation of colorectal cancer and glioma cells. This study was designed to further investigate the effects of TLA on the proliferation of hum...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(17)30085-7
更新日期：2017-08-01 00:00:00
abstract：:Neokotalanol, a potent α-glucosidase inhibitor isolated from Salacia reticulata, was synthesized through a key coupling reaction between a perbenzylated thiosugar and an appropriately protected perseitol triflate derived from D-mannose. This key step was found to be quite temperature dependent, and a simultaneous cycl...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(13)60079-5
更新日期：2013-11-01 00:00:00
abstract：:In the present study, two new acetylene conjugate compounds, dibutyl (2Z, 6Z)-octa-2, 6-dien-4-yne dioate (1), and dibutyl (2E, 6E)- octa-2, 6-dien-4-yne dioate (2), were isolated from the dry stem leaves of Viscum album, along with nine known compounds (3 - 11). Their structures were confirmed on the basis of spectro...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(19)30016-0
更新日期：2019-02-01 00:00:00
abstract：:In our previous study, we have elucidated the chemical profile of YGS40, a fraction of Yi-Gan San (YGS), used for the treatment of Alzheimer's disease (AD). Oxidative stress-induced apoptosis is implicated in neurodegenerative disorders such as AD. The aim of the present study was to explore the protective effects of ...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(15)30037-6
更新日期：2015-06-01 00:00:00
abstract：:Neurodegenerative diseases are the consequences of imbalance between the production of oxidative stress and its nullification by cellular defense mechanisms. Hydrogen peroxide (H2O2), a precursor of deleterious reactive oxygen species, elicits oxidative stress, resulting in severe brain injuries. Bacopa monnieri is we...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(18)30017-7
更新日期：2017-11-01 00:00:00
abstract：:Two new steroidal saponins, named timosaponin P (1) and timosaponin Q (2), were isolated from the rhizome parts of Anemarrhena asphodeloides Bunge using various chromatographic methods. Their structures and absolute configurations were elucidated by a combination of spectroscopic and spectrometric data, including 1D, ...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(17)30038-9
更新日期：2017-03-01 00:00:00
abstract：:The present study was designed to develop and evaluate glycyrrhetinic acid-graft-hyaluronic acid (HGA) conjugate for intravenous paclitaxel (PTX) delivery. Lyophilized PTX-loaded self-assembled HGA nanoparticles (PTX/HGAs) were prepared and characterized by dynamic light scattering measurements. Hemolysis test, intrav...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(15)30097-2
更新日期：2015-12-01 00:00:00
abstract：:The present study was designed to isolate and characterize the analgesic compounds of Artemisa sacrorum Ledeb. The EtOAc crude extracts from the aerial parts of Artemisa sacrorum Ledeb were separated by chromatography and the structures of new compounds were elucidated based on spectral analyses. Analgesic activities ...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(15)30080-7
更新日期：2015-10-01 00:00:00
abstract：:The flower buds of Lonicera macranthoides (Shan Yin-Hua), represent an important traditional Chinese medicine and food ingredient. A phytochemical investigation of the 70% EtOH extract of the flower buds of L. macranthoides resulted in the isolation of 12 triterpenoids (1-12), including two new ursane-type nortriterpe...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(19)30006-8
更新日期：2019-01-01 00:00:00
abstract：:Five new polyhydroxylated furostanol saponins were isolated from the roots and rhizomes of Tupistra chinensis, and their structures were determined as tupistrosides J-N (1-5), together with four known furostanol saponins (6-9), on the basis of physico-chemical properties and spectral analysis. Among them, compounds 3 ...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(19)30065-2
更新日期：2019-08-01 00:00:00
abstract：:Ge Gen Decoction (GGD), a Traditional Chinese Medicine prescription, is mainly used to treat infectious respiratory diseases and can relieve the symptoms of influenza A virus (IAV) infection. However, the underlying mechanism of GGD against IAV infection remains unclear. In this study, we found that GGD had moderate a...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(19)30079-2
更新日期：2019-09-01 00:00:00
abstract：:The stem of Cynomorium songaricum is a traditional Chinese medicine reputed to have tonic effects. C. coccineum growing in northern Africa and the Mediterranean region is regarded in Arabian medical practice as the "treasure of drugs". The major constituents of Cynomorium plants have been revealed to be phenolic compo...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章,评审
doi：10.1016/S1875-5364(13)60049-7
更新日期：2013-07-01 00:00:00
