Abstract:
:DNA repair by homologous recombination is highly suppressed in G1 cells to ensure that mitotic recombination occurs solely between sister chromatids. Although many homologous recombination factors are cell-cycle regulated, the identity of the events that are both necessary and sufficient to suppress recombination in G1 cells is unknown. Here we report that the cell cycle controls the interaction of BRCA1 with PALB2-BRCA2 to constrain BRCA2 function to the S/G2 phases in human cells. We found that the BRCA1-interaction site on PALB2 is targeted by an E3 ubiquitin ligase composed of KEAP1, a PALB2-interacting protein, in complex with cullin-3 (CUL3)-RBX1 (ref. 6). PALB2 ubiquitylation suppresses its interaction with BRCA1 and is counteracted by the deubiquitylase USP11, which is itself under cell cycle control. Restoration of the BRCA1-PALB2 interaction combined with the activation of DNA-end resection is sufficient to induce homologous recombination in G1, as measured by RAD51 recruitment, unscheduled DNA synthesis and a CRISPR-Cas9-based gene-targeting assay. We conclude that the mechanism prohibiting homologous recombination in G1 minimally consists of the suppression of DNA-end resection coupled with a multi-step block of the recruitment of BRCA2 to DNA damage sites that involves the inhibition of BRCA1-PALB2-BRCA2 complex assembly. We speculate that the ability to induce homologous recombination in G1 cells with defined factors could spur the development of gene-targeting applications in non-dividing cells.
journal_name
Naturejournal_title
Natureauthors
Orthwein A,Noordermeer SM,Wilson MD,Landry S,Enchev RI,Sherker A,Munro M,Pinder J,Salsman J,Dellaire G,Xia B,Peter M,Durocher Ddoi
10.1038/nature16142subject
Has Abstractpub_date
2015-12-17 00:00:00pages
422-6issue
7582eissn
0028-0836issn
1476-4687pii
nature16142journal_volume
528pub_type
杂志文章相关文献
NATURE文献大全abstract::Many marine invertebrates and fish respond to hydrostatic pressure in order to regulate their depth and synchronize their behaviour to tidal cycles. Here we investigate the effect of hydrostatic pressure on the vestibular hair cells located in the labyrinth of the dogfish Scyliorhinus canicula, and find that it modula...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/415495a
更新日期:2002-01-31 00:00:00
abstract::A large number of cis-regulatory sequences have been annotated in the human genome, but defining their target genes remains a challenge. One strategy is to identify the long-range looping interactions at these elements with the use of chromosome conformation capture (3C)-based techniques. However, previous studies lac...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature12644
更新日期:2013-11-14 00:00:00
abstract::Guanine-nucleotide exchange factors on ADP-ribosylation factor GTPases (ARF-GEFs) regulate vesicle formation in time and space by activating ARF substrates on distinct donor membranes. Mammalian GBF1 (ref. 2) and yeast Gea1/2 (ref. 3) ARF-GEFs act at Golgi membranes, regulating COPI-coated vesicle formation. In contra...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature05967
更新日期:2007-07-26 00:00:00
abstract::Climate change is predicted to increase both drought frequency and duration, and when coupled with substantial warming, will establish a new hydroclimatological model for many regions. Large-scale, warm droughts have recently occurred in North America, Africa, Europe, Amazonia and Australia, resulting in major effects...
journal_title:Nature
pub_type: 历史文章,杂志文章
doi:10.1038/nature11836
更新日期:2013-02-21 00:00:00
abstract::Dengue disease is caused by four different flavivirus serotypes, which infect 390 million people yearly with 25% symptomatic cases and for which no licensed vaccine is available. Recent phase III vaccine trials showed partial protection, and in particular no protection for dengue virus serotype 2 (refs 3, 4). Structur...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature14130
更新日期:2015-04-02 00:00:00
abstract::The T-cell receptor (TCR) repertoire is selected in the thymus after rearrangement of genes encoding TCR alpha and beta chains. Selection is based on the recognition by newly emergent T cells of self-ligands associated with molecules of the major histocompatibility complex: some combinations result in positive selecti...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/349531a0
更新日期:1991-02-07 00:00:00
abstract::Small synthetic molecules called growth-hormone secretagogues (GHSs) stimulate the release of growth hormone (GH) from the pituitary. They act through GHS-R, a G-protein-coupled receptor for which the ligand is unknown. Recent cloning of GHS-R strongly suggests that an endogenous ligand for the receptor does exist and...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/45230
更新日期:1999-12-09 00:00:00
abstract::Humans are able to classify novel items correctly by category; some other animals have also been shown to do this. During category learning, humans group perceptual stimuli by abstracting qualities from similarity relationships of their physical properties. Forming categories is fundamental to cognition and can be ind...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/35089076
更新日期:2001-08-16 00:00:00
abstract::Citrullination is the post-translational conversion of an arginine residue within a protein to the non-coded amino acid citrulline. This modification leads to the loss of a positive charge and reduction in hydrogen-bonding ability. It is carried out by a small family of tissue-specific vertebrate enzymes called peptid...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature12942
更新日期:2014-03-06 00:00:00
abstract::Stable associations of more than one species of symbiont within a single host cell or tissue are assumed to be rare in metazoans because competition for space and resources between symbionts can be detrimental to the host. In animals with multiple endosymbionts, such as mussels from deep-sea hydrothermal vents and ree...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/35077067
更新日期:2001-05-17 00:00:00
abstract::Crohn's disease is a debilitating inflammatory bowel disease (IBD) that can involve the entire digestive tract. A single-nucleotide polymorphism (SNP) encoding a missense variant in the autophagy gene ATG16L1 (rs2241880, Thr300Ala) is strongly associated with the incidence of Crohn's disease. Numerous studies have dem...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature13044
更新日期:2014-02-27 00:00:00
abstract::The heavy (H) and light (L) chains of antibodies consist of variable (V) and constant (C) regions. The V regions of the heavy and light chains form the antibody combining site. To determine whether a V region could be functional when joined to a polypeptide other than its own C region, we constructed a chimaeric gene ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/309364a0
更新日期:1984-05-24 00:00:00
abstract::In Dictyostelium discoideum, carbohydrate binding proteins (CBPs) or lectins have been implicated in the molecular basis of cellular cohesion. To determine the role of these CBPs, we have attempted to isolate structural gene mutants in which the CBPs have a defective affinity for carbohydrate ligands. We now report th...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/279215a0
更新日期:1979-05-17 00:00:00
abstract::The distribution of fitness effects of protein mutations is still unknown. Of particular interest is whether accumulating deleterious mutations interact, and how the resulting epistatic effects shape the protein's fitness landscape. Here we apply a model system in which bacterial fitness correlates with the enzymatic ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature05385
更新日期:2006-12-14 00:00:00
abstract::Galactose oxidase is an extracellular enzyme secreted by the fungus Dactylium dendroides. It is monomeric, with a relative molecular mass of 68,000, catalyses the stereospecific oxidation of a broad range of primary alcohol substrates and possesses a unique mononuclear copper site essential for catalysing a two-electr...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/350087a0
更新日期:1991-03-07 00:00:00
abstract::T lymphocytes of the alpha/beta T-cell receptor (TCR) lineage mature in the thymus, where they undergo a series of differentiation, expansion and selection events. For normal T-cell ontogeny to occur, thymocytes must interact physically with cortical and medullary thymic stroma cells. In parallel, interactions of the ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/373350a0
更新日期:1995-01-26 00:00:00
abstract::The mineral olivine dominates the composition of the Earth's upper mantle and hence controls its mechanical behaviour and seismic anisotropy. Experiments at high temperature and moderate pressure, and extensive data on naturally deformed mantle rocks, have led to the conclusion that olivine at upper-mantle conditions ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature03266
更新日期:2005-02-17 00:00:00
abstract::Truncation of the tumour suppressor adenomatous polyposis coli (Apc) constitutively activates the Wnt/beta-catenin signalling pathway. Apc has a role in development: for example, embryos of mice with truncated Apc do not complete gastrulation. To understand this role more fully, we examined the effect of truncated Apc...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature02028
更新日期:2003-10-09 00:00:00
abstract::The signal recognition particle (SRP) consists of one RNA and six protein subunits. The N-terminal domain of the 54K subunit contains a putative GTP-binding site, whereas the C-terminal domain binds signal sequences and SRP RNA. Binding of SRP to the signal sequence as it emerges from the ribosome creates a cytosolic ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/366351a0
更新日期:1993-11-25 00:00:00
abstract::This corrects the article DOI: 10.1038/nature22062. ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature24276
更新日期:2017-10-26 00:00:00
abstract::The structure of poly(dA).poly(dT) currently arouses great interest, mainly because dAn.dTn stretches are associated with considerable DNA bending. Until recently the heteronomous DNA described by Arnott et al., with the poly(dA) and poly(dT) chains in A and B conformations respectively, was the only detailed model of...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/325821a0
更新日期:1987-02-26 00:00:00
abstract::This corrects the article DOI: 10.1038/nature24277. ...
journal_title:Nature
pub_type: 杂志文章,已发布勘误
doi:10.1038/nature25160
更新日期:2018-01-25 00:00:00
abstract::Water masers are found in dense molecular clouds closely associated with supermassive black holes at the centres of active galaxies. On the basis of the understanding of the local water-maser luminosity function, it was expected that masers at intermediate and high redshifts would be extremely rare. However, galaxies ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature07544
更新日期:2008-12-18 00:00:00
abstract::Sex is expensive. A population of females that reproduce asexually should prima facie have twice the growth rate of an otherwise equivalent anisogamous sexual population lacking paternal care, or a population with modes of paternal care that can be co-opted by parthenogenetic females. The two leading theories for the ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/35079578
更新日期:2001-06-07 00:00:00
abstract::The battle for survival between bacteria and the viruses that infect them (phages) has led to the evolution of many bacterial defence systems and phage-encoded antagonists of these systems. Clustered regularly interspaced short palindromic repeats (CRISPR) and the CRISPR-associated (cas) genes comprise an adaptive imm...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature15254
更新日期:2015-10-01 00:00:00
abstract::Transforming growth factor-alpha (TGF-alpha) is a mitogenically active polypeptide hormone produced by acutely proliferating neoplastic and embryonic tissues. Molecular cloning of TGF-alpha cDNA from human and rat has indicated that this factor is synthesized as part of a larger precursor, proTGF-alpha. An intriguing ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/326883a0
更新日期:1987-04-30 00:00:00
abstract::Metastasis is the main cause of death in people with cancer. To colonize distant organs, circulating tumour cells must overcome many obstacles through mechanisms that we are only now starting to understand. These include infiltrating distant tissue, evading immune defences, adapting to supportive niches, surviving as ...
journal_title:Nature
pub_type: 杂志文章,评审
doi:10.1038/nature17038
更新日期:2016-01-21 00:00:00
abstract::Protonic ceramic fuel cells, like their higher-temperature solid-oxide fuel cell counterparts, can directly use both hydrogen and hydrocarbon fuels to produce electricity at potentially more than 50 per cent efficiency1,2. Most previous direct-hydrocarbon fuel cell research has focused on solid-oxide fuel cells based ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/s41586-018-0082-6
更新日期:2018-05-01 00:00:00
abstract::The geometry and dimensions of branched structures such as blood vessels or airways are important factors in determining the efficiency of physiological processes. It has been shown that fractal trees can be space filling and can ensure minimal dissipation. The bronchial tree of most mammalian lungs is a good example ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature02287
更新日期:2004-02-12 00:00:00
abstract::Although male parental care is rare among mammals, adult males of many cercopithecine primate species provide care for infants and juveniles. This care is often in the form of grooming, carrying, support in agonistic interactions, and protection against infanticide. For these behaviours to be interpreted as true paren...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature01866
更新日期:2003-09-11 00:00:00