Abstract：
:Piwi-interacting RNAs (piRNAs), a class of 26- to 32-nt non-coding RNAs (ncRNAs), function in germline development, transposon silencing, and epigenetic regulation. We performed deep sequencing and annotation of untreated and periodate-treated small RNA cDNA libraries from human fetal and adult germline and reference somatic tissues. This revealed abundant piRNAs originating from 150 piRNA-encoding genes, including some exhibiting gender-specific expression, in fetal ovary and adult testis-developmental periods coinciding with mitotic cell divisions expanding fetal germ cells prior to meiotic divisions. The absence of reads mapping uniquely to annotated piRNA genes demonstrated their paucity in fetal testis and adult ovary and absence in somatic tissues. We curated human piRNA-expressing regions and defined their precise borders and observed piRNA-guided cleavage of transcripts antisense to some piRNA-producing genes. This study provides insights into sex-specific mammalian piRNA expression and function and serves as a reference for human piRNA analysis and annotation.
journal_name
Cell Repjournal_title
Cell reportsauthors
Williams Z,Morozov P,Mihailovic A,Lin C,Puvvula PK,Juranek S,Rosenwaks Z,Tuschl Tdoi
10.1016/j.celrep.2015.09.030subject
Has Abstractpub_date
2015-10-27 00:00:00pages
854-863issue
4issn
2211-1247pii
S2211-1247(15)01041-4journal_volume
13pub_type
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