Abstract：
:Mesenchymal stem cells (MSCs) are the common precursors of several functionally disparate cell lineages. A plethora of chemical and physical stimuli contribute to lineage decisions and guidance, including mechanical stretch concomitant with physical movement. Here, we examined how stretch regulates MSC differentiation into adipocytes and the intracellular signaling pathways involved. MSCs were cultured under adipogenic conditions and divided into a control and an experimental group. Cultures in the experimental group were subjected to a sinusoidal stretch regimen delivered via flexible culture bottoms (5% magnitude, 10 times per min, 6h/day, 3 or 5 days). Expression levels of the adipocyte markers PPARγ-2, adiponectin, and C/EBPα were measured as indices of differentiation. Compared to controls, MSCs exposed to mechanical stretch exhibited downregulated PPARγ-2, adiponectin, and C/EBPα mRNA expression. Alternatively, stretch upregulated phosphorylation of Smad2. This stretch-induced increase in Smad2 phosphorylation was suppressed by pretreatment with the TGFβ1/Smad2 pathway antagonist SB-431542. Pretreatment with the TGFβ1/Smad2 signaling agonist TGFβ1 facilitated the inhibitory effect of stretch on the expression levels of PPARγ-2, adiponectin, and C/EBPα proteins, while pretreatment with SB-431542 reversed the inhibitory effects of subsequent stretch on the expression levels of these markers. These results strongly suggest that the anti-adipogenic effects of mechanical stretch on MSCs are mediated, at least in part, by activation of the TGFβ1/Smad2 signaling pathway.
journal_name
J Biomechjournal_title
Journal of biomechanicsauthors
Li R,Liang L,Dou Y,Huang Z,Mo H,Wang Y,Yu Bdoi
10.1016/j.jbiomech.2015.08.013subject
Has Abstractpub_date
2015-10-15 00:00:00pages
3665-71issue
13eissn
0021-9290issn
1873-2380pii
S0021-9290(15)00452-2journal_volume
48pub_type
杂志文章abstract：:High knee joint-loading increases the risk and progression of knee osteoarthritis (OA). Mechanical loading on the knee is reflected in the external knee adduction moment (KAdM) that can be measured during gait with laboratory-based measurement systems. However, clinical application of these systems is limited. Ambulat...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2012.09.030
更新日期：2013-01-04 00:00:00
abstract：:The mechanisms by which mechanical loading may alter bone development within growth plates are still poorly understood. However, several growth plate cell or tissue morphological parameters are associated with both normal and mechanically modulated bone growth rates. The aim of this study was to quantify in situ the t...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2010.05.010
更新日期：2010-09-17 00:00:00
abstract：:Arterial embolism is responsible for the death of lots of people who suffers from heart diseases. The major risk of embolism in upper limbs is that the ruptured particles are brought into the brain, thus stimulating neurological symptoms or causing the stroke. We presented a computational model using fluid-structure i...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2012.01.020
更新日期：2012-04-30 00:00:00
abstract：:Computation of muscle excitation patterns that produce coordinated movements of muscle-actuated dynamic models is an important and challenging problem. Using dynamic optimization to compute excitation patterns comes at a large computational cost, which has limited the use of muscle-actuated simulations. This paper int...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/s0021-9290(02)00432-3
更新日期：2003-03-01 00:00:00
abstract：:This paper presents a novel inverse estimation approach for the active contraction stresses of tongue muscles during speech. The proposed method is based on variational data assimilation using a mechanical tongue model and 3D tongue surface shapes for speech production. The mechanical tongue model considers nonlinear ...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2017.09.008
更新日期：2017-11-07 00:00:00
abstract：:The aim of this study is to investigate the biomechanics for orthodontic tooth movement (OTM) subjected to concurrent single-tooth vibration (50Hz) with conventional orthodontic force application, via a clinical study and computational simulation. Thirteen patients were recruited in the clinical study, which involved ...
journal_title：Journal of biomechanics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.jbiomech.2017.06.012
更新日期：2017-07-26 00:00:00
abstract：:Sphenodon, a lizard-like reptile, is the only living representative of a group that was once widespread at the time of the dinosaurs. Unique jaw mechanics incorporate crushing and shearing motions to breakdown food, but during this process excessive loading could cause damage to the jaw joints and teeth. In mammals li...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2010.08.001
更新日期：2010-12-01 00:00:00
abstract：:The role of pulsatile unloading in hemodynamic changes in intraventricular flow and ventricular wall stress remains unknown. In this study, a finite element model of the left ventricle (LV) is proposed to calculate the mechanical response. The constitutive model of the LV is composed of a quasi-incompressible transver...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2019.109425
更新日期：2020-04-16 00:00:00
abstract：:The purpose of this work was to establish a controlled and reversible muscle weakness model for studying the effects of weakness on joint degeneration leading to osteoarthritis (OA). The knee extensor muscles of rabbits were injected with single or repeat doses of Botulinum type-A toxin (BTX-A) to partially inhibit ac...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2007.03.001
更新日期：2007-01-01 00:00:00
abstract：:Force plates for human movement analysis provide accurate measurements when mounted rigidly on an inertial reference frame. Large measurement errors occur, however, when the force plate is accelerated, or tilted relative to gravity. This prohibits the use of force plates in human perturbation studies with controlled s...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2018.05.009
更新日期：2018-06-25 00:00:00
abstract：INTRODUCTION:The postnatal heart grows mostly in response to increased hemodynamic load. However, the specific biomechanical stimuli that stimulate cardiac growth as a reaction to increased hemodynamic load are still poorly understood. It has been shown that isolated neonatal rat cardiac myocytes normalize resting sarc...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2011.11.028
更新日期：2012-03-15 00:00:00
abstract：:This paper presents a new direct method for estimating the average center of rotation (CoR). An existing least-squares (LS) solution has been shown by previous works to have reduced accuracy for data with small range of motion (RoM). Alternative methods proposed to improve the CoR estimation use iterative algorithms. ...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2006.05.010
更新日期：2007-01-01 00:00:00
abstract：:Structural properties of articular cartilage such as proteoglycan content, collagen content and collagen alignment are known to vary over length scales as small as a few microns (Bullough and Goodfellow, 1968; Bi et al., 2006). Characterizing the resulting variation in mechanical properties is critical for understandi...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2009.10.012
更新日期：2010-03-03 00:00:00
abstract：:This study looks at pulsatile blood flow through four different right coronary arteries, which have been reconstructed from biplane angiograms. A non-Newtonian blood model (the Generalised Power Law), as well as the usual Newtonian model of blood viscosity, is used to study the wall shear stress in each of these arter...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2005.01.034
更新日期：2006-01-01 00:00:00
abstract：:This study proposed a revision to the Rosenstein's method of numerical calculation of the largest Lyapunov exponent (LyE) to make it more robust to noise. To this aim, the effect of increasing number of initial neighboring points on the LyE value was investigated and compared to values obtained by filtering the time s...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2019.01.013
更新日期：2019-03-06 00:00:00
abstract：:Lower propulsive joint powers, particularly at the ankle, are often observed in older compared to young adults. Interventions to increase joint powers often require labs with motion capture and force treadmill technology. Translating these interventions out of the lab requires identifying portable measures that track ...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2020.109821
更新日期：2020-06-09 00:00:00
abstract：:Although we sometimes use the intrasynovial tendon allograft as a donor, the gliding ability of allograft prepared by lyophilization is significantly decreased. The gliding ability of the grafted tendon after tendon reconstruction is very important because the high gliding resistance causes more adhesion and leads to ...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2010.12.030
更新日期：2011-06-03 00:00:00
abstract：:Quantifying angular joint kinematics of the upper body is a useful method for assessing upper limb function. Joint angles are commonly obtained via motion capture, tracking markers placed on anatomical landmarks. This method is associated with limitations including administrative burden, soft tissue artifacts, and int...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2018.02.028
更新日期：2018-04-27 00:00:00
abstract：:The ability to quantitatively measure stability is essential to ensuring the safety of locomoting systems. While the response to perturbation directly reflects the stability of a motion, this experimental method puts human subjects at risk. Unfortunately, existing indirect methods for estimating stability from unpertu...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2018.02.022
更新日期：2018-04-27 00:00:00
abstract：:The structural properties of tendons and ligaments are integral to their ability to function effectively in vivo and are determined from both the geometrical form and material properties. Many studies of tendon and ligament include a mechanical assessment of the structure in vitro. However, to determine the material p...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2004.05.003
更新日期：2005-03-01 00:00:00
abstract：:The trunk has a multi-segmental structure and is composed of the cervical, thoracic, and lumber spines and surrounding soft tissue elements; this allows flexible deformation during dynamic movements. The purpose of this study was to quantitatively assess trunk deformation during dynamic movement. Ten male subjects per...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2017.04.019
更新日期：2017-07-05 00:00:00
abstract：:Surface EMG was recorded in four subjects on three different occasions from the three parts of the deltoid, the clavicular part of the pectoralis major and from the infraspinatus muscles at different angles of abduction, in the frontal and scapular plane. The integrated EMG was related to the maximum values found for ...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/0021-9290(85)90037-5
更新日期：1985-01-01 00:00:00
abstract：:Animal models of ventricular tachypacing (VTP) have been successfully used to reproduce the relevant features observed in patients with atrial fibrillation, such as increased atrial pressure and volume, ion-channel alterations and fibrosis. After performing VTP on a healthy Yorkshire pig, we measured an increase in vo...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2011.09.003
更新日期：2011-11-10 00:00:00
abstract：:The purpose of the present study was to investigate the rotational kinematics pattern of the upper trunk and the pelvis and the complexity-variability of their movement, during the sprint butterfly stroke between male and female swimmers with long fins (18-26 cm) and without fins. Two pairs of 3D accelerometers and gy...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2016.01.004
更新日期：2016-02-08 00:00:00
abstract：:Upper extremity musculoskeletal disorders represent an important health issue across all industry sectors; as such, the need exists to develop models of the hand that provide comprehensive biomechanics during occupational tasks. Previous optical motion capture studies used a single marker on the dorsal aspect of finge...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2011.04.003
更新日期：2011-06-03 00:00:00
abstract：:The ability to monitor the healing of bone fractures is crucially important in their treatment. The aim of the present study was to develop and validate an objective method for monitoring fracture healing based on bone vibrational response. An analytic model was formulated, with which the mechanical parameters at the ...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/0021-9290(90)90060-g
更新日期：1990-01-01 00:00:00
abstract：:Soft tissue artefact (STA) and marker placement variability are sources of error when measuring the intrinsic kinematics of the foot. This study aims to demonstrate a non-invasive, combined ultrasound and motion capture (US/MC) technique to directly measure foot skeletal motion. The novel approach is compared to a sta...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2013.12.014
更新日期：2014-03-21 00:00:00
abstract：:The nonlinear stress-strain characteristics of the human menisci were determined by uniaxial elongation tests performed on circumferential and radial specimens prepared from different regions, layers, and locations. The properties of the collagen fibers and the matrix were calculated using the test results along with ...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/0021-9290(94)00081-e
更新日期：1995-04-01 00:00:00
abstract：:Previous models to predict breast movement whilst performing physical activities have, erroneously, assumed uniform elasticity within the breast. Consequently, the predicted displacements have not yet been satisfactorily validated. In this study, real time motion capture of the natural vibrations of a breast that foll...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2017.11.027
更新日期：2018-01-23 00:00:00
abstract：:The understanding of biomechanical deficits and impaired neural control of gait after stroke is crucial to prescribe effective customized treatments aimed at improving walking function. Instrumented gait analysis has been increasingly integrated into the clinical practice to enhance precision and inter-rater reliabili...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2017.08.006
更新日期：2017-10-03 00:00:00
