Abstract:
:The catalytic subunit of DNA-dependent protein kinase (DNA-PKcs) is a critical component of the non-homologous end-joining pathway of DNA double-stranded break repair. DNA-PKcs has also been shown recently functioning in mitotic regulation. Here, we report that DNA-PKcs negatively regulates the stability of Cyclin B1 protein through facilitating its ubiquitination mediated by Cdh1 / E 3 ubiquitin ligase APC/C pathway. Loss of DNA-PKcs causes abnormal accumulation of Cyclin B1 protein. Cyclin B1 degradation is delayed in DNA-PKcs-deficient cells as result of attenuated ubiquitination. The impact of DNA-PKcs on Cyclin B1 stability relies on its kinase activity. Our study further reveals that DNA-PKcs interacts with APC/C core component APC2 and its co-activator Cdh1. The destruction of Cdh1 is accelerated in the absence of DNA-PKcs. Moreover, overexpression of exogenous Cdh1 can reverse the increase of Cyclin B1 protein in DNA-PKcs-deficient cells. Thus, DNA-PKcs, in addition to its direct role in DNA damage repair, functions in mitotic progression at least partially through regulating the stability of Cyclin B1 protein.
journal_name
Int J Biol Scijournal_title
International journal of biological sciencesauthors
Shang ZF,Tan W,Liu XD,Yu L,Li B,Li M,Song M,Wang Y,Xiao BB,Zhong CG,Guan H,Zhou PKdoi
10.7150/ijbs.12443subject
Has Abstractpub_date
2015-07-14 00:00:00pages
1026-35issue
9issn
1449-2288pii
ijbsv11p1026journal_volume
11pub_type
杂志文章abstract::Sepsis-induced myocardial dysfunction (SIMD) is a life-threatening complication caused by inflammation, but how it is initiated is still unclear. Several studies have shown that extracellular high mobility group box 1 (HMGB1), an important cytokine triggering inflammation, is overexpressed during the pathogenesis of S...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.50186
更新日期:2020-09-19 00:00:00
abstract::Spinal cord injury (SCI) induces the disruption of the blood-spinal cord barrier (BSCB) which leads to infiltration of blood cells, an inflammatory response, and neuronal cell death, resulting spinal cord secondary damage. Retinoic acid (RA) has a neuroprotective effect in both ischemic brain injury and SCI, however t...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.13229
更新日期:2016-01-01 00:00:00
abstract::Background: The monitoring and management of blood glucose concentration are standard practices in critical settings as hyperglycaemia has been shown close association with poorer outcomes. Several meta-analyses have revealed that intensive glucose control has no benefit in decreasing short-term mortality among critic...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.43447
更新日期:2020-03-12 00:00:00
abstract::Different stimuli often activate the same intracellular signaling molecules but trigger distinct cell responses. We explored whether or not MAPK signaling induced by macrophage colony-stimulating factor (M-CSF), which is responsible for osteoclast proliferation, differs from that induced by receptor activator of NF-κB...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.13814
更新日期:2016-01-01 00:00:00
abstract::Melanoma is an aggressive form of skin cancer characterized by rapid invasion and metastasis. CD147 is known to be functioning in cell invasion. In this study, we showed that CD147 was translocated from the cell membrane to the mitochondria in advanced melanoma. Melanoma patients with CD147 localized to the mitochondr...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.52043
更新日期:2021-01-01 00:00:00
abstract:BACKGROUND:Oridonin (ORI) could inhibit the proliferation and induce apoptosis in various cancer cell lines. However, the mechanism is not fully understood. METHODS:Human prostate cancer (HPC) cells were cultured in vitro and cell viability was detected by the CCK-8 assay. The ultrastructure changes were observed unde...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.4554
更新日期:2012-01-01 00:00:00
abstract::This study aims to investigate the effect and mechanism of type 2 voltage-gated chloride channel (ClC-2) on myelin development of newborn rats' cerebral white matter with gestational diabetes mellitus (GDM). In this study, GDM model was induced in late pregnant rat model. The alteration of ClC-2 expression in various ...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.17716
更新日期:2017-01-15 00:00:00
abstract::Angiogenesis plays an important role in myocardial repair after myocardial infarction (MI). Cardiac micro-vascular endothelial cells (CMECs) are important participants in myocardial angiogenesis processes. Recent studies have revealed that Nuclear factor-erythroid 2-related factor 2 (Nrf2), a master transcription fact...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.5887
更新日期:2013-07-07 00:00:00
abstract::We collected clinical data from 308 prostate cancer (PCa) patients to investigate the clinical characteristics and independent risk factors of bone metastasis (BM) and to establish a prediction model for BM of PCa and determine the necessity of bone scans. Univariate and multivariate analyses were performed based on a...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.27537
更新日期:2019-01-01 00:00:00
abstract::Protein phosphatase 2A (PP2A) is a major protein phosphatase with important cell functions. Known and utilized as a potent inhibitor of PP2A, microcystin-LR (MCLR) targets PP2A as a core element that affects numerous cellular mechanisms. But apart from direct inhibition, the exact effect of MCLR on PP2A in cell is lar...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.7.740
更新日期:2011-01-01 00:00:00
abstract::The standard of care for patients who suffer from non-organ confined prostate cancer (CaP) is androgen deprivation therapy (ADT). ADT exploits the reliance of CaP cells on androgen receptor (AR) signaling throughout CaP progression from androgen-stimulated (AS) to castration-recurrent (CR) disease. AR is a member of t...
journal_title:International journal of biological sciences
pub_type: 杂志文章,评审
doi:10.7150/ijbs.8479
更新日期:2014-06-01 00:00:00
abstract::The genetic landscape of pancreatic cancer shows nearly ubiquitous mutations of K-RAS. However, oncogenic K-Ras(mt) alone is not sufficient to lead to pancreatic ductal adenocarcinoma (PDAC) in either human or in genetically modified adult mouse models. Many stimulants, such as high fat diet, CCK, LPS, PGE2 and others...
journal_title:International journal of biological sciences
pub_type: 杂志文章,评审
doi:10.7150/ijbs.15020
更新日期:2016-01-28 00:00:00
abstract::Focal adhesion kinase (FAK) is a major signaling molecule which functions downstream of integrins or in conjunction with mitogenic signaling pathways. FAK is overexpressed and/or activated in many types of human tumors, in which it promotes cell adhesion, survival, migration and invasion. In addition to FAK's ability ...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.10273
更新日期:2015-02-17 00:00:00
abstract::Mast cells are found abundant at sites of acupoints. Nerve cells share perivascular localization with mast cells. Acupuncture (mechanical stimuli) can activate mast cells to release adenosine triphosphate (ATP) which can activate nerve cells and modulates pain-processing pathways in response to acupuncture. In this pa...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.8631
更新日期:2014-04-26 00:00:00
abstract::The vertebrate body plan externally is largely symmetrical across the midline but internal organs develop asymmetrically. The biological basis of asymmetric organ development has been investigated extensively for years, although the proposed mechanisms remain controversial. By comparison, the biological origin of exte...
journal_title:International journal of biological sciences
pub_type: 杂志文章,评审
doi:10.7150/ijbs.9886
更新日期:2014-09-10 00:00:00
abstract::The objective of this study was to study the effect of diabetic hyperglycemia on astrocytes after forebrain ischemia. Streptozotocin (STZ)-injected hyperglycemic and vehicle-injected normoglycemic rats were subjected to 15 minutes of forebrain ischemia. The brains were harvested in sham-operated controls and in animal...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.7251
更新日期:2013-09-25 00:00:00
abstract::Dihydropyrimidinase-related protein 4 (Dpysl4) is a known regulator of hippocampal neuron development. Here, we report that Dpysl4 is involved in growth regulation, polarization and differentiation of dental epithelial cells during tooth germ morphogenesis. A reduction in Dpysl4 gene expression in the tooth germ produ...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.5510
更新日期:2013-04-26 00:00:00
abstract::Dendritic cells (DCs) are the most potent specialized antigen-presenting cells as now known, which play a crucial role in initiating and amplifying both the innate and adaptive immune responses. Immunologically, the motilities and T cell activation capabilities of DCs are closely related to the resulting immune respon...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.33412
更新日期:2019-05-20 00:00:00
abstract::The gene encoding PDX1 (pancreatic duodenum homeobox 1), the main transcription factor regulating the glucose-dependent transactivation of the insulin promoter in pancreatic beta-cells, clusters with two closely related homeobox genes (Gsh1 and Cdx2/3), all of them belonging to the ParaHox gene family. The ParaHox gen...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.4.48
更新日期:2008-02-06 00:00:00
abstract::Background and aims: Dysfunction of the immune regulatory system plays a role in the pathogenesis of allergic rhinitis (AR). The underlying mechanism needs to be further investigated. Published data indicate that soluble CD83 (sCD83) has immune regulatory activities. This study aims to investigate the role of sCD83 in...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.38722
更新日期:2020-01-01 00:00:00
abstract:OBJECTIVES:Reactive oxygen species (ROS) induced lipid peroxidation is associated with sperm function. Malondialdehyde (MDA) concentration and glutathione peroxidase (GPx) activity represent the lipid peroxidation and spermicidal antioxidant, respectively. We aimed to evaluate the relationship of MDA and GPx levels wit...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.2.23
更新日期:2006-01-01 00:00:00
abstract::Odor-detection in the malaria mosquito Anopheles gambiae involves large families of diverse proteins, including multiple odorant binding proteins (AgOBPs) and olfactory receptors (AgORs). The receptors AgOR1 and AgOR2, as well as the binding protein AgOBP1, have been implicated in the recognition of human host odors. ...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.6.614
更新日期:2010-10-08 00:00:00
abstract::Many organisms in extremely cold environments such as the Antarctic Pole have evolved antifreeze molecules to prevent ice formation. There are four types of antifreeze proteins (AFPs). Type-IV antifreeze proteins (AFP4s) are present also in certain temperate and even tropical fish, which has raised a question as to wh...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.9126
更新日期:2014-06-24 00:00:00
abstract::Reverting activated hepatic stellate cells (HSCs) to less activation or quiescent status is a promising strategy for liver fibrosis. Histone deacetylase inhibitor (HDACI) could suppress HSCs activation. Our previous study demonstrated a critical role of miRNAs in HSCs activation. Here, we explored the involvement of m...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.28642
更新日期:2019-01-06 00:00:00
abstract::Cell density has profound impacts on the cell culture practices of human pluripotent stem cells. The regulation of cell growth, cell death, pluripotency and differentiation converge at high density, but it is largely unknown how different regulatory mechanisms act at this stage. We use a chemically defined medium to s...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.24681
更新日期:2018-04-05 00:00:00
abstract::Pacemaker activity of the sinoatrial node has been studied extensively in various animal species, but is virtually unexplored in man. As such, it is unknown whether the fast sodium current (I(Na)) plays a role in the pacemaker activity of the human sinoatrial node. Recently, we had the unique opportunity to perform pa...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.5.201
更新日期:2009-01-01 00:00:00
abstract::Excessive inflammation induced by various risk factors is associated with the development of bronchopulmonary dysplasia (BPD). Caffeine exerts potent anti-inflammatory effects as a clinical preventive medicine for BPD. Recently, NLRP3 inflammasome activation has been demonstrated to be essential for the pathogenesis o...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.34211
更新日期:2019-06-02 00:00:00
abstract::D-Xylitol is found in low content as a natural constituent of many fruits and vegetables. It is a five-carbon sugar polyol and has been used as a food additive and sweetening agent to replace sucrose, especially for non-insulin dependent diabetics. It has multiple beneficial health effects, such as the prevention of d...
journal_title:International journal of biological sciences
pub_type: 杂志文章,评审
doi:10.7150/ijbs.6.834
更新日期:2010-12-15 00:00:00
abstract::Pork quality is an economically important trait and one of the main selection criteria for breeding in the swine industry. In this genome-wide association study (GWAS), 455 pigs from a porcine Large White × Minzhu intercross population were genotyped using the Illumina PorcineSNP60K Beadchip, and phenotyped for intram...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.3614
更新日期:2012-01-01 00:00:00
abstract::Dengue virus (DENV) causes the most prevalent arthropod-borne viral disease of humans worldwide. Glycosphingolipids (GSLs) are involved in virus infection by regulating various steps of viral-host interaction. However, the distinct role of GSLs during DENV infection remains unclear. In this study, we used mouse melano...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.15641
更新日期:2016-05-25 00:00:00