Abstract:
:Nonsteroidal anti-inflammatory drugs (NSAIDs) are widely used therapeutic agents; however, their pharmacological actions raise concerns about potential risks to the reproductive health of aquatic vertebrates. In the present study, a medaka ovulation assay was applied as an in vitro model to evaluate NSAID-induced antiovulatory activity. We first tested five NSAIDs, including diclofenac sodium (DCF), ketoprofen (KP), salicylic acid (SA), mefenamic acid (MA), and acetylsalicylic acid (ASA) for their antiovulatory activities toward the follicles isolated from the ovaries of spawning females. Of all the chemicals tested, DCF had the highest antiovulatory activity, with the concentration that caused 50% inhibition (IC50) (101 µM). MA was the second most potent inhibitor following DCF, but KP, SA, or ASA had little inhibitory effect on the ovulation of the follicles. The in vitro antiovulatory activity of five NSAIDs showed good correlation with data published on the inhibitory activity on human COX-2. Second, we selected DCF and SA as the most and least potent NSAIDs, respectively, and examined the effects on reproduction of intact fish in order to evaluate whether the ovulation assay was a reasonable predictor of potential reproductive effects in fish. Females exposed to DCF showed a concentration-dependent decrease in the number of spawned eggs and an increment in the gonadosomatic index (GSI), possibly due to an anovulation in the females. In contrast, neither fecundity nor the GSI of females decreased at up to 20 mg/L of SA, at which acute lethality to medaka was induced. In conclusion, the medaka ovulation assay reflected the potency of NSAID-induced antiovulatory activity and may thus serve as an in vitro model for the prediction of NSAID-induced reproductive toxicity. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1710-1719, 2016.
journal_name
Environ Toxicoljournal_title
Environmental toxicologyauthors
Yokota H,Eguchi S,Hasegawa S,Okada K,Yamamoto F,Sunagawa A,Tanaka M,Yamamoto R,Nakano Edoi
10.1002/tox.22173subject
Has Abstractpub_date
2016-12-01 00:00:00pages
1710-1719issue
12eissn
1520-4081issn
1522-7278journal_volume
31pub_type
杂志文章abstract::Particulate matter the environmental toxicant, with a diameter less than or equal to 2.5 μm (PM2.5 ) is a common cause of several respiratory diseases. In recent years, several studies have suggested that PM2.5 can influence diverse diseases, such as respiratory diseases, cardiovascular diseases, metabolic diseases, d...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22591
更新日期:2018-11-01 00:00:00
abstract::The prostate is an accessory reproductive gland that is sensitive to the action of exogenous compounds known as endocrine disrupters that alter normal hormonal function. Finasteride is a widely used chemical that acts to inhibit the conversion of testosterone in its most active form, dihydrotestosterone. It is known t...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22838
更新日期:2020-01-01 00:00:00
abstract::The aim of this study was to evaluate whether the enzyme-linked immunosorbent assay (ELISA) anti-Adda technique could be used to monitor free microcystins (MCs) in biological samples from fish naturally exposed to toxic cyanobacteria by using receiver operating characteristic (ROC) curve software to establish an optim...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20528
更新日期:2011-02-01 00:00:00
abstract::Tris (1,3-dichloro-2-propyl) phosphate (TDCIPP) is one of the widely used organophosphorus flame retardants (OPFRs), which are regarded as suitable substitutes for brominated flame retardants (BFRs). Previously, we have validated the toxicity of TDCIPP in PC12 cells owing to the induced alterations in GAP43, NF-H, CaM...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22401
更新日期:2017-06-01 00:00:00
abstract::Rheumatoid arthritis (RA) is the most common type of chronic inflammatory disease which is triggered by dysfunction in the immune system which in turn affects synovial joints. Current treatment of RA with NSAIDs and DMRDs is limited by their side effect. As a result, the interest in alternative, well tolerated anti-in...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22264
更新日期:2017-02-01 00:00:00
abstract::T-2 toxin is the most toxic among mycotoxins and poses a potential health hazard for both humans and animals. At high doses, T-2 toxin can cause shock-like syndrome that can result in death. We evaluated the effect of time course and route of exposure on hepatic oxidative damage in mice and it is only such study so fa...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.21895
更新日期:2015-01-01 00:00:00
abstract::Multi-walled carbon nanotubes (MWCNTs) have many applications in industry and used as additives in polymers, catalysts, anodes in lithium-battery and drug delivery. There is little information about MWCNTs' (210 nm) genotoxic potential on juvenile freshwater fish Channa punctatus. Therefore, in this study, we have det...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22871
更新日期:2020-03-01 00:00:00
abstract::Quinolines are aromatic nitrogen compounds with wide therapeutic potential to treat parasitic and microbial diseases. In this study, the genotoxicity of quinoline, 4-methylquinoline, 4-nitroquinoline-1-oxide (4-NQO), and diversely functionalized quinoline derivatives and the influence of the substituents (functional g...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.21905
更新日期:2015-03-01 00:00:00
abstract::The aim of this study is to determine the chemical composition, and evaluate the genotoxic, and anti-growth potency of the methanol extracts of lichen species Hypogymnia physodes (L.) Nyl. (HPE). Anti-growth effect was tested in two different human breast cancer cell lines (MCF-7 and MDA-MB-231) by the MTT and ATP via...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.21809
更新日期:2014-05-01 00:00:00
abstract::Chronic inhalation bioassays in rodents are used to assess pulmonary carcinogenicity for purposes of hazard identification and potentially for risk characterization. Numerous studies have been confirmed that exposure to titanium dioxide nanoparticles (TiO2 NPs) may result in chronic pulmonary inflammation in both mice...
journal_title:Environmental toxicology
pub_type: 杂志文章,收录出版
doi:10.1002/tox.22393
更新日期:2017-05-01 00:00:00
abstract::Females are born with a finite number of oocyte-containing follicles and ovary damage results in reduced fertility. Cadmium accumulates in the reproductive system, damaging it, and the cigarette smoke is a potential exposure route. Natural therapies are relevant to health benefits and disease prevention. This study ve...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22225
更新日期:2017-01-01 00:00:00
abstract::The aim of this study was to investigate the signaling pathways involved in the cyclooxygenase (COX)-2 regulation induced by nonylphenol (NP) in mouse testis Sertoli TM4 cells. Our results showed that treatment of TM4 cells with NP increased COX-2 protein expression and interleukin-6 (IL)-6 and prostaglandin E2 (PGE2)...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.21987
更新日期:2015-09-01 00:00:00
abstract::The roles of long noncoding RNA (lncRNA) MACC1-AS1 have been revealed in various tumors. This work aims to explore the roles of lncRNA MACC1-AS1 in the stemness of nonsmall cell lung cancer (NSCLC) cells and the underlying mechanism. We showed that overexpression of MACC1-AS1 enhanced the stemness of NSCLC cells, whic...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22936
更新日期:2020-09-01 00:00:00
abstract::Endotoxins exist anywhere including in water pools, dust, humidifier systems, and machining fluids. The major causal factor is endotoxins in many serious diseases, such as fever, sepsis, multi-organ failure, meningococcemia, and severe morbidities like neurologic disability, or hearing loss. Endotoxins are also called...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22033
更新日期:2016-02-01 00:00:00
abstract::So far, little is known on the distribution of hepatotoxic microcystin (MC) in various organs of bivalves, and there is no study on MC accumulation in bivalves from Chinese waters. Distribution pattern and seasonal dynamics of MC-LR, -YR and -RR in various organs (hepatopancreas, intestine, visceral mass, gill, foot, ...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20146
更新日期:2005-12-01 00:00:00
abstract::The mycotoxin citrinin (CTN), a natural contaminant in foodstuffs and animal feeds, exerts cytotoxic and genotoxic effects on various mammalian cells. CTN causes cell injury, including apoptosis. Previous studies by our group showed that CTN triggers apoptosis in mouse embryonic stem cells, as well as embryonic develo...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20434
更新日期:2009-08-01 00:00:00
abstract::Oral submucous fibrosis (OSF) has been indicated as one of the oral potentially malignant disorders. Epidemiological studies have attributed this pathological fibrosis to the habit of areca nuts chewing, which causes chronic inflammation and persistent activation of myofibroblasts in the oral cavity. Hence, it is cruc...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22489
更新日期:2018-01-01 00:00:00
abstract::Particulate matter 2.5 (PM2.5)-induced pulmonary inflammation has become a public concern in recent years. In which, the activation of the NLRP3/caspase-1 pathway was closely related to the inflammatory response of various diseases. However, the promotion effect of the NLRP3/caspase-1 pathway on PM2.5-induced pulmonar...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.23035
更新日期:2020-09-30 00:00:00
abstract::Doxorubicin (DOX)-induced cardiomyopathy is a lethal disease. DOX-induced cardiotoxic effects are attributed towards increased redox status and apoptotic signaling. In this study, we show that genistein offers protection against DOX-induced cardio toxicity in the mice model. DOX-mediated increase in serum cardiac trop...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22730
更新日期:2019-05-01 00:00:00
abstract::Culture-independent methods employed in fungal genetic studies using in vitro amplification (PCR) and analysis of specific genes or gene fragments have proved to be useful for detection, identification, and molecular taxonomy of various plant pathogens including Fusarium spp. This approach may be faster than culture-d...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20193
更新日期:2006-08-01 00:00:00
abstract::Casticin, a polymethoxyflavone, is one of the major active components obtained from Fructus viticis, which have been shown to have anticancer activities including induce cell apoptosis in human cancer cells. The aim of this study was to investigate the molecular mechanisms by which casticin inhibits cell migration and...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22417
更新日期:2017-09-01 00:00:00
abstract::Polychlorinated biphenyls (PCBs) are a class of organic pollutants that have been linked to pancreatic disease. However, their role in affecting the exocrine function of pancreas and the underlying mechanism remains elusive. In the present study, male C57 mice were treated with Aroclor 1254, a commercially available P...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22079
更新日期:2016-06-01 00:00:00
abstract::PM10 was collected in a Milan urban site, representative of the city air quality, during winter and summer 2006. Mean daily PM10 concentration was 48 μg m(-3) during summer and 148 μg m(-3) during winter. Particles collected on Teflon filters were chemically characterized and the endotoxin content determined by the ...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20611
更新日期:2012-02-01 00:00:00
abstract::Polybrominated diphenyl ethers (PBDEs) are ubiquitous and prolific contaminant in both the abiotic and biotic environment because of the wide industrial applications of these chemicals. In the present study, the effects of 2,2',4,4'-tetrabrominateddiphenyl ether (BDE-47) and 2,2',4,4',5,5'-hexabromodiphenyl ether (BDE...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22985
更新日期:2020-11-01 00:00:00
abstract::Adrenomedullin (AdM) is synthesized and secreted by a number of cells and tissue. AdM is a potent vasodilator but it is also considered a neuromodulator, an angiogenic factor, and a hormone regulator. AdM possess antiapoptotic, antioxidant, and antimicrobial properties. Heavy metals such as cadmium and lead are found ...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20430
更新日期:2009-06-01 00:00:00
abstract::Ecological risk assessments are, in part, based on results of toxicity tests conducted under standard exposure conditions. Global climate change will have a wide range of effects on estuarine habitats, including potentially increasing water temperature and salinity, which may alter the risk assessment of estuarine pol...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20726
更新日期:2013-07-01 00:00:00
abstract::Antioxidative enzymes (superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX)), as well as lipid peroxidation and proline were studied in moss Brachythecium piligerum Card. collected from different sites in Shanghai, China, to validate the physiological parameters as biomarkers of atm...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20571
更新日期:2011-10-01 00:00:00
abstract::There is increasing worldwide interest in developing of markers for tumor diagnosis and identification of individuals who are at high cancer risk. Cancer, like other diseases accompanied by metabolic disorders, causes characteristic effects on cell turnover rate, activity of modifying enzymes, and RNA/DNA modification...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.21970
更新日期:2015-07-08 00:00:00
abstract::Since the early 20th century, hairdressers (HD) have been exposed to a wide range of harmful chemical products. To determine the possible genetic damage to HD, as a result of their occupational exposure to combinations of different chemical factors, we applied the micronucleus assay on their peripheral blood lymphocyt...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20579
更新日期:2011-10-01 00:00:00
abstract::Environmental stress factors may be responsible for biological changes in living species that are able to overcome deleterious effects depending on their detoxifying capacities. Defense systems present in every living species are involved in elimination of reactive chemical species of endogenous or exogenous origin, n...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20024
更新日期:2004-08-01 00:00:00