Abstract:
:The aim of this study was to evaluate whether the enzyme-linked immunosorbent assay (ELISA) anti-Adda technique could be used to monitor free microcystins (MCs) in biological samples from fish naturally exposed to toxic cyanobacteria by using receiver operating characteristic (ROC) curve software to establish an optimal cut-off value for MCs. The cut-off value determined by ROC curve analysis in tench (Tinca tinca) exposed to MCs under laboratory conditions by ROC curve analysis was 5.90-μg MCs/kg tissue dry weight (d.w.) with a sensitivity of 93.3%. This value was applied in fish samples from natural ponds (Extremadura, Spain) in order to asses its potential MCs bioaccumulation by classifying samples as either true positive (TP), false positive (FP), true negative (TN), or false negative (FN). In this work, it has been demonstrated that toxic cyanobacteria, mainly Microcystis aeruginosa, Aphanizomenon issatchenkoi, and Anabaena spiroides, were present in two of these ponds, Barruecos de Abajo (BDown) and Barruecos de Arriba (BUp). The MCs levels were detected in waters from both ponds with an anti-MC-LR ELISA immunoassay and were of similar values (between 3.8-6.5-μg MC-LR equivalent/L in BDown pond and 4.8-6.0-μg MC-LR equivalent/L in BUp). The MCs cut-off values were applied in livers from fish collected from these two ponds using the ELISA anti-Adda technique. A total of 83% of samples from BDown pond and only 42% from BUp were TP with values of free MCs higher than 8.8-μg MCs/kg tissue (d.w.).
journal_name
Environ Toxicoljournal_title
Environmental toxicologyauthors
Moreno IM,Herrador MÁ,Atencio L,Puerto M,González AG,Cameán AMdoi
10.1002/tox.20528subject
Has Abstractpub_date
2011-02-01 00:00:00pages
45-56issue
1eissn
1520-4081issn
1522-7278journal_volume
26pub_type
杂志文章abstract::Long-term exposure of agriculturally used organochloride and organophosphate pesticides have been shown to cause long-lasting hematotoxicity and increased incidence of aplastic anemia in humans. The mechanisms involved in pesticide induced hematotoxicity and the features of toxicity that may play a major role in bone ...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20775
更新日期:2014-01-01 00:00:00
abstract::The aim of this research was to analyze the effects and the modes of action of elemental sulfur (S(0)) in bioluminescence and respiration of Vibrio fischeri cells and the enzymes crude luciferase, pure catalase, and alcohol dehydrogenase (ADH). Metallic copper removed sulfur and reduced the toxicity of acetone extract...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20025
更新日期:2004-08-01 00:00:00
abstract::The environmental pollutant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is known to affect bone metabolism. We evaluated the protective effects of the triterpene glycoside actein from the herb black cohosh against TCDD-induced toxicity in MC3T3-E1 osteoblastic cells. We found that TCDD significantly reduced cell viabil...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22459
更新日期:2017-12-01 00:00:00
abstract::Effects of perchlorate on sodium-iodide symporter (NIS) and pendrin gene expression in deer mice kidney and stomach were investigated. This was accomplished by isolating a partial cDNA sequence of deer mice NIS gene of 425 bps, and quantitatively analyzing NIS mRNA expression in various deer mouse tissues. The highest...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20271
更新日期:2007-08-01 00:00:00
abstract:OBJECTIVES:We evaluated the role of oxidative stress in Sudan IV-induced DNA damage, using human liver-derived HepG2 cells. METHODS:The DNA damaging effects of Sudan IV in HepG2 cells were evaluated by alkaline single cell gel electrophoresis assay and micronucleus test (MNT). To clarify the underlying mechanisms, we ...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20558
更新日期:2011-06-01 00:00:00
abstract::Dicrotophos is a systemic insecticide with a wide range of applications. We investigated the genotoxicity of dicrotophos using the Ames test, the chromosome aberration test in CHO-K1 cells, and the comet assay in the Hep G2 cells, while this chemicals' toxicity to both the cell lines was evaluated with the MTT assay. ...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20645
更新日期:2012-05-01 00:00:00
abstract::Structure-activity relationships for oestrogenicity were developed based on 120 aromatic chemicals evaluated in the Saccharomyces cerevisiae-based Lac-Z reporter assay. Relative gene activation was compared to 17 beta-estradiol and varied over eight orders of magnitude. Analysis of the data compared to 17 beta-estradi...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.10027
更新日期:2002-02-01 00:00:00
abstract::Smoking increases the risk of cardiovascular disorders and leads to damage caused by inflammation and oxidative stress. The actin cytoskeleton is a key player in the response to inflammatory stimuli and is an early target of cellular oxidative stress. The purpose of this study was to investigate the changes in actin c...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22133
更新日期:2016-11-01 00:00:00
abstract::Acetaminophen (APAP) is one of the few recommended analgesic and antipyretic drugs in some critical cases such as viral disease COVID-19. However, the unrestricted use of APAP develops liver disorders. Hepatotoxicity and liver injury can also be induced by ionizing radiation (IR) during radiotherapy. The data of the c...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.23077
更新日期:2020-12-17 00:00:00
abstract::8-Hydroxy-2'-deoxyguanosine (8-OHdG) is the most common biomarker of oxidative DNA damage, it is formed by chemical carcinogens and can be measured in any species. Perfluorooctanoic acid (PFOA) and perfluorononanoic acid (PFNA) are suspected genotoxic carcinogens through induction of reactive oxygen species that are r...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22034
更新日期:2016-02-01 00:00:00
abstract::The herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) has been widely used in agriculture and forestry since the 1940s. 2,4-D has been shown to produce a wide range of adverse effects-from embryotoxicity and teratogenicity to neurotoxicity-on animal and human health. The purpose of this study was to determine the possi...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20690
更新日期:2013-01-01 00:00:00
abstract::Andrographolide, the main bioactive component of the medicinal plant Andrographis paniculata, has been shown to possess potent anti-inflammatory activity. Endothelin 1 (ET-1), a potent vasoconstrictor peptide produced by vascular endothelial cells, displays proinflammatory property. Hypoxia-inducible factor 1α (HIF-1α...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22293
更新日期:2017-03-01 00:00:00
abstract::Titanium dioxide nanoparticles (TiO2 NPs) have been used in environmental management, food, medicine, and industry. But TiO2 NPs have been demonstrated to cross the blood-brain barrier and store up in the brain organization, leading to glutamate-mediated neurotoxicity. However, the neurotoxicity in the brain is not we...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22077
更新日期:2016-06-01 00:00:00
abstract::Particulate matter 2.5 (PM2.5)-induced pulmonary inflammation has become a public concern in recent years. In which, the activation of the NLRP3/caspase-1 pathway was closely related to the inflammatory response of various diseases. However, the promotion effect of the NLRP3/caspase-1 pathway on PM2.5-induced pulmonar...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.23035
更新日期:2020-09-30 00:00:00
abstract::Effect-directed analysis (EDA) has been reported to be a powerful tool for the identification of the responsible toxicants in contaminated, hazardous environmental samples. The aim of this study was to investigate whether it also is possible to use currently available EDA methodology to identify potentially relevant t...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20135
更新日期:2005-10-01 00:00:00
abstract::The acute toxicity of the insecticide fenitrothion was measured using four freshwater algae (Chlorella saccharophila, Chlorella vulgaris, Scenedesmus acutus, and Scenedesmus subspicatus) and one cyanobacteria (Pseudanabaena galeata). Insecticide concentrations eliciting 50% growth reduction over 96 hr (EC50) ranged fr...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.1038
更新日期:2001-01-01 00:00:00
abstract::Irradiation of polycyclic aromatic hydrocarbons (PAHs) in aqueous solution with simulated solar radiation (SSR; a light source with a visible light: UV-A:UV-B ratio similar to that of sunlight) can greatly enhance their toxicity. Two microbial toxicity tests with Vibrio fischeri were used to investigate the effect of ...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.1034
更新日期:2001-06-01 00:00:00
abstract::Females are born with a finite number of oocyte-containing follicles and ovary damage results in reduced fertility. Cadmium accumulates in the reproductive system, damaging it, and the cigarette smoke is a potential exposure route. Natural therapies are relevant to health benefits and disease prevention. This study ve...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22225
更新日期:2017-01-01 00:00:00
abstract::Quinolines are aromatic nitrogen compounds with wide therapeutic potential to treat parasitic and microbial diseases. In this study, the genotoxicity of quinoline, 4-methylquinoline, 4-nitroquinoline-1-oxide (4-NQO), and diversely functionalized quinoline derivatives and the influence of the substituents (functional g...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.21905
更新日期:2015-03-01 00:00:00
abstract::Antioxidative enzymes (superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX)), as well as lipid peroxidation and proline were studied in moss Brachythecium piligerum Card. collected from different sites in Shanghai, China, to validate the physiological parameters as biomarkers of atm...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20571
更新日期:2011-10-01 00:00:00
abstract::The freshwater crabs Sinopotamon henanense were exposed to different concentrations of waterborne cadmium (Cd). The relationship between tissue-specific Cd accumulation and metallothionein (MT) induction was investigated using the Cd saturation assay and atomic absorption spectrophotometry method. The results showed t...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20339
更新日期:2008-06-01 00:00:00
abstract::Hydroponics studies were conducted to investigate the antioxidant adaptations, ascorbate and glutathione metabolism in hyperaccumulating ecotype of Sedum alfredii (HE) exposed to high Cd environment, when compared with its nonhyperaccumulating ecotype (NHE). Exposure to Cd induced a burst of oxidative stress in both e...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20362
更新日期:2008-08-01 00:00:00
abstract::Acute toxicities of perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) were tested on four freshwater species and three plant species. PFOS was more toxic than PFOA for all species tested in this study. Similar time-response patterns of PFOS and PFOA toxicity were observed for each tested species. Valu...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20396
更新日期:2009-02-01 00:00:00
abstract::Global use of azole fungicides is expected to increase over the next several years. Triticonazole is a triazole fungicide that is used for turf protection, residential, and other commercial applications. As such, it can enter local rural and urban water systems via run-off and rain events. Early life stages of aquatic...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22862
更新日期:2020-02-01 00:00:00
abstract::Long non-coding RNA (lncRNA) plays a critical role in male germline development. Atrazine (AZ) as an environmental endocrine disrupting chemical (EDCs) can induce male reproductive toxicity in amphibians. Our previous studies demonstrated that AZ can alter gene and circular RNA (circRNA) expression of damaged testes i...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22704
更新日期:2019-04-01 00:00:00
abstract::Acute severe carbon monoxide (CO) poisoning induces hypoxia that leads to cardiovascular and nervous systems disturbances. Different complex mechanisms lead to CO neurotoxicity including lipid peroxidation, inflammatory and immune-mediated reactions, myelin degeneration and finally neuronal apoptosis and necrosis. Gra...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22210
更新日期:2017-01-01 00:00:00
abstract::Endotoxins exist anywhere including in water pools, dust, humidifier systems, and machining fluids. The major causal factor is endotoxins in many serious diseases, such as fever, sepsis, multi-organ failure, meningococcemia, and severe morbidities like neurologic disability, or hearing loss. Endotoxins are also called...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22033
更新日期:2016-02-01 00:00:00
abstract::There is increasing worldwide interest in developing of markers for tumor diagnosis and identification of individuals who are at high cancer risk. Cancer, like other diseases accompanied by metabolic disorders, causes characteristic effects on cell turnover rate, activity of modifying enzymes, and RNA/DNA modification...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.21970
更新日期:2015-07-08 00:00:00
abstract::Trichloroethylene (TCE) is ubiquitous in our living environment, and prenatal exposure to TCE is reported to cause congenital heart disease in humans. Although multiple studies have been performed using animal models, they have limited value in predicting effects on humans due to the unknown species-specific toxicolog...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22142
更新日期:2016-11-01 00:00:00
abstract::Fluorescent pseudomonads were a major component [log (10) 4.2-6.1 colony-forming units mL-1] of the culturable heterotrophic gram-negative bacterioplankton observed in three Mississippi Delta oxbow lakes in this study. Pure cultures of fluorescent pseudomonads were isolated from three Mississippi Delta oxbow lakes (18...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/1522-7278(2001)16:1<9::aid-tox20>3.0.co;2-
更新日期:2001-01-01 00:00:00