Abstract:
:Acute severe carbon monoxide (CO) poisoning induces hypoxia that leads to cardiovascular and nervous systems disturbances. Different complex mechanisms lead to CO neurotoxicity including lipid peroxidation, inflammatory and immune-mediated reactions, myelin degeneration and finally neuronal apoptosis and necrosis. Granulocyte colony-stimulating factor (G-CSF) is considered to be a novel neuroprotective agent. In this study, we evaluated the efficacy of G-CSF therapy on CO neurotoxicity in rats with acute CO poisoning. Rats were exposed to 3000 ppm CO in air (0.3%) for 1 h, and then different doses (50,100, and 150 µg/kg) of G-CSF or normal saline were administrated intraperitoneally. Water content of brain as an indicator for total edema and blood brain barrier integrity (Evans blue extravasation) were evaluated. Malondialydehyde was determined in order to evaluate the effect of G-CSF on CO-induced lipid peroxidation in brain tissues. Also, the effect of G-CSF on myeloperoxidase activity in the brain tissue was evaluated. The effect of G-CSF administration on induced apoptosis in the brain was measured using TUNEL method. To evaluate the level of MBP, STAT3 and pSTAT3 and HO-1 proteins and the effect of G-CSF on these proteins Western blotting was carried out. G-CSF reduced water content of the edematous poisoned brains (100 µg/kg) and BBB permeability (100 and 150 µg/kg) (P < 0.05). G-CSF (150 µg/kg) reduced the MDA level in the brain tissues (P < 0.05 as compared to CO poisoned animals). G-CSF did not decrease the MPO activity after CO poisoning in any doses. G-CSF significantly reduced the number of apoptotic neurons and Caspase 3 protein levels in the brain. Western blotting results showed that G-CSF treatment enhanced expression of HO-1 and MBP, STAT3 and pSTAT3 proteins in the brain tissues. Based on our results, a single dose of G-CSF immediately after CO poisoning significantly attenuates CO neurotoxicity via different mechanisms. © 2015 Wiley Periodicals, Inc. Environ Toxicol 32: 37-47, 2017.
journal_name
Environ Toxicoljournal_title
Environmental toxicologyauthors
Ghorbani M,Mohammadpour AH,Abnous K,Movassaghi AR,Sarshoori JR,Shahsavand S,Hashemzaei M,Moallem SAdoi
10.1002/tox.22210subject
Has Abstractpub_date
2017-01-01 00:00:00pages
37-47issue
1eissn
1520-4081issn
1522-7278journal_volume
32pub_type
杂志文章abstract::The incidence of type 1 diabetes (T1D) and its associated risks of chronic kidney disease or end-stage renal disease development are on the rise. T1D is an autoimmune disease in which insulin-producing beta cells are destroyed. Increased incidence of T1D has been suggested to be a result of environmental factors such ...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22857
更新日期:2020-02-01 00:00:00
abstract::Casticin, a polymethoxyflavone, is one of the major active components obtained from Fructus viticis, which have been shown to have anticancer activities including induce cell apoptosis in human cancer cells. The aim of this study was to investigate the molecular mechanisms by which casticin inhibits cell migration and...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22417
更新日期:2017-09-01 00:00:00
abstract::Acetaminophen (AP) is a widely used, cheap, and over-the-counter nonsteroidal anti-inflammatory drug. Its toxicity depends on the cytochrome P-450 (CYP)-mediated oxidation to the toxic metabolite N-acetyl-p-benzoquinoneimine. On the other hand, arsenic, a global groundwater and environmental contaminant of major publi...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20551
更新日期:2011-06-01 00:00:00
abstract::Organic wastewater contaminants (OWCs) have recently been documented in several aquatic ecosystems. We examined the effects of three OWCs (caffeine, acetaminophen, and triclosan) on the activity level, startle response, survivorship, and growth of northern leopard frog (Rana pipiens) tadpoles. We conducted two experim...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20017
更新日期:2004-06-01 00:00:00
abstract::Environmental stress factors may be responsible for biological changes in living species that are able to overcome deleterious effects depending on their detoxifying capacities. Defense systems present in every living species are involved in elimination of reactive chemical species of endogenous or exogenous origin, n...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20024
更新日期:2004-08-01 00:00:00
abstract::Perfluorooctane sulfonate (PFOS) is an animal carcinogen. However, the underlying mechanism in cancer initiation is still largely unknown. Recently identified microRNAs (miRNAs) may play an important role in toxicant exposure and in the process of toxicant-induced tumorigenesis. We used PFOS to investigate PFOS-induce...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.21949
更新日期:2015-05-01 00:00:00
abstract::Tris (1,3-dichloro-2-propyl) phosphate (TDCIPP) is one of the widely used organophosphorus flame retardants (OPFRs), which are regarded as suitable substitutes for brominated flame retardants (BFRs). Previously, we have validated the toxicity of TDCIPP in PC12 cells owing to the induced alterations in GAP43, NF-H, CaM...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22401
更新日期:2017-06-01 00:00:00
abstract::An enormous quantity of pharmacologically active principles are currently being introduced into the environment, with consequent escalation of environmental problems, but only a small number of studies are focusing on an assessment of their genotoxic effects. The aim of this article is to assess the genotoxic effects ...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20685
更新日期:2012-10-01 00:00:00
abstract::Widespread occupational and environmental exposure to benzene is unavoidable and poses a public health threat. Studies of potential interventions to prevent or relieve benzene toxicity are, thus, essential. Research has shown l-carnitine (LC) has beneficial effects against various pathological processes and diseases. ...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22939
更新日期:2020-10-01 00:00:00
abstract::The roles of long noncoding RNA (lncRNA) MACC1-AS1 have been revealed in various tumors. This work aims to explore the roles of lncRNA MACC1-AS1 in the stemness of nonsmall cell lung cancer (NSCLC) cells and the underlying mechanism. We showed that overexpression of MACC1-AS1 enhanced the stemness of NSCLC cells, whic...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22936
更新日期:2020-09-01 00:00:00
abstract::Fascinating super paramagnetic uniqueness of iron oxide particles at nano-scale level make them extremely useful in the state of the art therapies, equipments, and techniques. Cobalt ferrite (CoFe2 O4 ) magnetic nanoparticles (MNPs) are extensively used in nano-based medicine and electronics, results in extensive disc...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22206
更新日期:2016-12-01 00:00:00
abstract::The toxicity and chemical quality of surface water and sediment in the River Traun in Austria were studied because of recurrent fish mortality in some alpine rivers over the last few years. The analyses were carried out on samples collected during winter and summer upstream and downstream of two municipal wastewater t...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20027
更新日期:2004-08-01 00:00:00
abstract::Colorectal cancer (CC) is an important human malignancy with high cancer related death worldwide. The chemotherapy using doxorubicin hydrochloride is one of the most common cancer therapeutic methods. However, drug resistance lowers the treatment efficacy in CC patients. The combination therapies seem to be more promi...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.23105
更新日期:2021-01-28 00:00:00
abstract::Microcystins (MCs), as the most dominant bloom-forming strains in eutrophic surface water, can induce hepatotoxicity by oxidative stress. Alpha-lipoic acid (α-LA) is a super antioxidant that can induce the synthesis of antioxidants, such as glutathione (GSH), by nuclear factor erythroid 2-related factor 2 (Nrf2). Howe...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22908
更新日期:2020-07-01 00:00:00
abstract::Fluorescent pseudomonads were a major component [log (10) 4.2-6.1 colony-forming units mL-1] of the culturable heterotrophic gram-negative bacterioplankton observed in three Mississippi Delta oxbow lakes in this study. Pure cultures of fluorescent pseudomonads were isolated from three Mississippi Delta oxbow lakes (18...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/1522-7278(2001)16:1<9::aid-tox20>3.0.co;2-
更新日期:2001-01-01 00:00:00
abstract::A high throughput culture methodology of unicellular eukaryote Tetrahymena pyriformis, strain GL were used for the determination of catecholamines toxicity and their metabolism. Catecholamines exhibited acute toxicity to Tetrahymena cells where dopamine and L-DOPA showed higher toxic potential of EC(10) (0.39 and 0.63...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20457
更新日期:2009-12-01 00:00:00
abstract::Micronucleus induction by the diarrhetic shellfish toxin okadaic acid (OA) was investigated in two intestinal models, cultured human Caco-2 cells and colon epithelial cells of mice treated in vivo. Exposure to OA for 4 and 24 h induced dose-responsive increases in the frequency of micronucleated Caco-2 cells; the mini...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20154
更新日期:2006-02-01 00:00:00
abstract::Renal cell carcinoma (RCC) is the most common type of kidney cancer in adults and the major cause of mortality in urological cancer. Most patients with RCC are asymptomatic until the disease is advanced and unresectable. In this situation, systemic therapy with immunotherapy or molecularly targeted therapy agents play...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22411
更新日期:2017-07-01 00:00:00
abstract::Toxins in cyanobacteria are a recognized risk in the treatment of drinking-water treatment. Cyanotoxins can occur in two modifications: cell bound and dissolved in water. The process of toxin release may occur naturally, but it also may be induced through the processes of drinking-water treatment. Both causes of relea...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.10067
更新日期:2002-01-01 00:00:00
abstract::Polybrominated diphenyl ethers (PBDEs) are ubiquitous and prolific contaminant in both the abiotic and biotic environment because of the wide industrial applications of these chemicals. In the present study, the effects of 2,2',4,4'-tetrabrominateddiphenyl ether (BDE-47) and 2,2',4,4',5,5'-hexabromodiphenyl ether (BDE...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22985
更新日期:2020-11-01 00:00:00
abstract::Microcystins are a family of potent hepatotoxins produced by freshwater cyanobacteria and can cause animal intoxications and human diseases. In this study, the effect of microcystin-LR (MC-LR) on the tissues of freshwater pearl mussel (Hyriopsis cumingii) was evaluated and differentially expressed genes in the hepatop...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20652
更新日期:2012-07-01 00:00:00
abstract::Tributyltin (TBT), a well-known endocrine disruptor, is an omnipresent environmental pollutant and is explicitly used in many industrial applications. Previously we have shown its neurotoxic potential on cerebral cortex of male Wistar rats. As the effect of TBT on other brain regions is not known, we planned this stud...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22009
更新日期:2015-12-01 00:00:00
abstract::Manganese (Mn) plays a critical role in individual growth and development, yet excessive exposure can result in neurotoxicity, especially cognitive impairment. Neuronal apoptosis is considered as one of the mechanisms of Mn-induced neurotoxicity. Recent evidence suggests that cAMP-PKA-CREB signaling regulates apoptosi...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22776
更新日期:2019-09-01 00:00:00
abstract::Andrographolide, the main bioactive component of the medicinal plant Andrographis paniculata, has been shown to possess potent anti-inflammatory activity. Endothelin 1 (ET-1), a potent vasoconstrictor peptide produced by vascular endothelial cells, displays proinflammatory property. Hypoxia-inducible factor 1α (HIF-1α...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22293
更新日期:2017-03-01 00:00:00
abstract::Three methods of algal assays--the standard assay, microassay, and the proposed fluorescence assay--are compared from the point of view of reliability of EC50 detection, the minimum required time for the detection, sensitivity of individual measurement, i.e. at which cell density the particular assay can be used for E...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20516
更新日期:2010-12-01 00:00:00
abstract::Antioxidative enzymes (superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX)), as well as lipid peroxidation and proline were studied in moss Brachythecium piligerum Card. collected from different sites in Shanghai, China, to validate the physiological parameters as biomarkers of atm...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20571
更新日期:2011-10-01 00:00:00
abstract::This work investigated the effects of mercury chloride (HgCl2 ) acute exposure on virgin, pregnant and lactating rats by determination of renal and hepatic morphological and ultrastructural parameters and the expression of oxidative stress and stress tolerance markers, due to kidney and liver are the organs that more ...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22370
更新日期:2017-05-01 00:00:00
abstract::Effects of perchlorate on sodium-iodide symporter (NIS) and pendrin gene expression in deer mice kidney and stomach were investigated. This was accomplished by isolating a partial cDNA sequence of deer mice NIS gene of 425 bps, and quantitatively analyzing NIS mRNA expression in various deer mouse tissues. The highest...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20271
更新日期:2007-08-01 00:00:00
abstract::Oral cancer is one of the cancer-related diseases in human populations and its incidence rates are rising worldwide. Fisetin, a flavonoid from natural products, has been shown to exhibit anticancer activities in many human cancer cell lines but the molecular mechanism of fisetin-induced apoptosis in human oral cancer ...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22396
更新日期:2017-06-01 00:00:00
abstract::Cardiomyocyte apoptosis is the major risk factor for the development of heart failure (HF). The purpose of this study was to evaluate the effects of Gamma-aminobutyric acid (GABA) tea on hypertension-induced cardiac apoptotic pathways in spontaneously hypertensive rats (SHR). In order to reveal the mechanisms, 36 male...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22565
更新日期:2018-07-01 00:00:00