Abstract：
:Here, we investigated the quantitative and qualitative differences in antibody classes and subclasses in serum immune complexes (ICs) of Visceral Leishmaniasis (VL), Post Kala-azar Dermal Leishmaniasis (PKDL) and different cross reactive diseases like Malaria, Leprosy, Vitiligo as compared to control subjects. IC levels were measured through a newly developed PEG ELISA, using L. donovani promastigote membrane antigen coated plate. Antibody classes and subclasses were identified using polyspecific sera and monoclonal antibodies, respectively. ICs were purified using polyethylene glycol (PEG) precipitation. Conditional logistic regression showed an association between IgG1-containing ICs and increased risk of PKDL (OR = 75, P < 0.05) and an association of IgG-containing ICs with VL (OR = 621, P = 0.001). PEG ELISA demonstrated almost 13-15 fold higher IgG containing ICs titers in VL as compared to control (P < 0.001). The assay further established a significant (P < 0.05) difference in the IgG containing ICs titers between VL and PKDL. The isolated ICs were further analyzed by subjecting them to one-dimensional PAGE and subsequently stained with combination of periodic acid schiff (PAS) with silver. A differential banding pattern between VL and PKDL was obtained. Four distinct bands with carbohydrate rich glycoconjugates were identified in PKDL ICs, which were absent in VL and control group. It suggests the scope for developing a novel differential diagnostic assay.
journal_name
Indian J Exp Bioljournal_title
Indian journal of experimental biologyauthors
Datta S,Modak D,Sarkar S,Saha B,Mukhopadhyay Ssubject
Has Abstractpub_date
2015-06-01 00:00:00pages
321-8issue
6eissn
0019-5189issn
0975-1009journal_volume
53pub_type
杂志文章abstract：:Diazepam (10 mg/kg, ip) treatment was given from day 13 to 20 of gestation to pregnant rats, this being the critical period for neural development in this species. The pups born were subjected to open-field exploratory behaviour, tunnel-board exploratory behaviour, elevated zero maze behaviour and social interaction t...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1996-11-01 00:00:00
abstract：:Supplementation of cyanate in rats caused a significant decrease in serum GSH and increase in calcium and phosphate level both in serum and lens. Consequently, these changes led to induce acidosis uremia in serum and hypercalcemia and hyperphosphatemia in lens which may be possible causing factor for cataract. ...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:Cockroaches are inhabitant of sewers and frequent visitors of kitchen and stores in the night to feed on left over. It is liable to disseminate a number of pathogens by contaminating kitchen surface, feeding vessels and food items left open. Salmonella paratyphi B var Java a common pathogen of man and animal was used ...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1995-05-01 00:00:00
abstract：:Leprosy bacillus (LB) and leprosy derived in vitro culture forms, the chemoautotrophic nocardioform (CAN) bacteria, showed an extremely close homology and identity with each other as regards a chemoautotrophic nutritional pattern, a nocardioform morphology, a weak acid-fastness coupled with Gram and Gomori's stain pos...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章,评审
doi：
更新日期：2001-10-01 00:00:00
abstract：:The ELISA and GM1-ELISA, by using antiserum to purified Salmonella enterotoxin (SE), were standardized and carried out to screen salmonellae isolated from foods of animal origin for enterotoxigenicity. Of the 101 strains of Salmonella belonging to 15 different serogroups tested, 76 (75.24%) strains from 13 serogroups ...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1991-10-01 00:00:00
abstract：:The effect of the oral administration of megestrol acetate (MA; 17 alpha-acetoxy-6-dehydro-6-methylprogesterone) for 30 days at a dose of 40 mg/kg body weight/day on the genital organs and fertility of male rats was studied. MA had no effect on spermatogenesis or the fertility of the animals. However, the weights of...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1965-10-01 00:00:00
abstract：:Orange peel oil is used extensively as an approved flavour enhancer in fruit drinks, carbonated beverages and as a scenting agent in soaps and cosmetics. Limonene, which is a monocyclic monoterpene is present in orange peel oil from 90 to 95% (w/w). Monoterpenes have been shown to be very effective chemopreventive age...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2002-03-01 00:00:00
abstract：:Biochemical estimation of acidic and basic proteins of chick gastrocnemii (G. externus, G. medius and G. internus) and pectoralis muscles has been done under normal, denervated and work stress conditions from 1-56 days of postnatal growth. The reciprocal relationship of the two protein groups is clearly established. I...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1992-05-01 00:00:00
abstract：:Crayfish exoskeleton (CE) samples are generally less invasive and easy to be collected. However, it is difficult to extract DNA from them. This study was intended to investigate CE as a DNA source and design an easy and efficient DNA extraction protocol for polymerase chain reactions. Specific primer pair (PPO-F, PPO-...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2011-12-01 00:00:00
abstract：:The implantation failure in newly inseminated mice induced by food deprivation for 48 hr, beginning at 0900 hrs on day 4 post coitum, was prevented by simultaneous exposure to light continuously for 48 or 36 hr. Food-deprived females that were exposed to continuous light for 36 hr showed a significant increase in feta...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1995-04-01 00:00:00
abstract：:Effects of a selective monoamine oxidase (MAO)--A inhibitor, clorgyline, a selective MAO-B inhibitor, deprenyl, and a non-selective MAO inhibitor, nialamide, were investigated on footshock-induced aggression (FIA) in paired rats. The doses and pretreatment times of the inhibitors used were based on an earlier reported...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1990-08-01 00:00:00
abstract：:Regulation of siderophore production in response to iron concentration in the medium was examined. Threshold concentration was recorded for twenty fungi and three rhizobacterial pseudomonads. Organisms showed difference in threshold values at which they stopped siderophore elaboration. In nine fungi (3 aspergilli, 1 p...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2000-03-01 00:00:00
abstract：:Protective efficacy of DL-alpha lipoic acid on adriamycin induced hepatotoxicity was evaluated in rats. Adriamycin toxicity, induced by a single injection (ip; 15 mg/kg body wt), was expressed by an elevation in alanine transaminase, aspartate transaminase, bilirubin levels in serum and alkaline phosphatase, lactate d...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2007-12-01 00:00:00
abstract：:The present study has been undertaken to observe the effect of aqueous extract of M. oleifera (MO) leaf (300mg/kg body weight) on mean ulcer index, enterochromaffin (EC) cells and serotonin (5-hydroxytryptamine; 5-HT) content of ulcerated gastric tissue. Ulceration was induced by using aspirin (500 mg/kg, po), cerebel...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2007-08-01 00:00:00
abstract：:Study of antitumor and radiosensitizing properties of W. somnifera (Ashwagandha), a well known medicinal plant, have yielded encouraging results. The alcoholic extract of the dried roots of the plant as well as the active component withaferin A isolated from the extract showed significant antitumor and radiosensitizin...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章,评审
doi：
更新日期：1996-10-01 00:00:00
abstract：:Wide spread use of Di-(2-ethylhexyl) phthalate (DEHP) has made it a ubiquitous contaminant in today's environment, responsible for possible carcinogenic and endocrine disrupting effects. In the present investigation an integrative toxicoproteomic approach was made to study the estrogenic potential of DEHP. In vitro ex...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2014-11-01 00:00:00
abstract：:gamma-Glutamyl transpeptidase (gamma-GT) activity in various organs of mice during ontogenesis has been determined. The activity profile in foetus, neonates and the adult animal were compared to ascertain the functional significance of the enzyme. In addition, the effect of cortisol on gamma-GT activity in adult kidne...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1991-12-01 00:00:00
abstract：:Spirulina platensis treatment (400 mg kg(-1) for 25 days) effectively suppressed peripheral sensitization via modulation of glial activation and improved motor coordination and restoration of functional motor activity in collagen-induced arthritic rats. Spirulina treatment also resulted in an appreciable reduction of ...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2011-10-01 00:00:00
abstract：:With respect to 31 different selected test bacteria, all sensitive to benzyl penicillin (Pc), ampicillin (Ap), methicillin (Me), ceporan (Ce), cloxacillin (Cx), streptomycin (Sm), kanamycin (Km), gentamicin (Gm), chloramphenicol (Cm), tetracycline (Tc), polymyxin (Pm) as well as ambodryl [Am; an antihistamine (bromodi...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1990-03-01 00:00:00
abstract：:Incidence of obesity and diabetes is increasing at an alarming rate not only among the populations of the affluent nations but also amongst the populations of the developing nations. Understanding the mechanisms that cause the onset of these pathological conditions is a requisite to effectively tackling this problem. ...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章,评审
doi：
更新日期：2000-09-01 00:00:00
abstract：:Rate of heat production during cell proliferation following UV-irradiation of respiratory-deficient yeast cells was measured as a function of time (p-t curve) in a batch microcalorimeter. Following observations were made: (a) All growing cell cultures showed 3 distinct phases of heat production namely lag, exponential...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1990-08-01 00:00:00
abstract：:A new design of insecticide testing assembly has been devised that is highly versatile in testing the efficacy, potency and interaction of insecticides and other test chemicals. The merits of the assembly and technique have been explained. ...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2002-05-01 00:00:00
abstract：:Sulforaphane, a constituent of broccoli was investigated for its antimutagenic potential against different classes of cooked food mutagens (heterocyclic amines). These include imidazoazaarenes such as 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), 2-amino-3,4-dimethylimidazo[4,5-f]quinoline (MeIQ), 2-amino-3,8-dimethyl...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2003-03-01 00:00:00
abstract：:Mechanisms of interleukin-18 (IL-18) and interleukin-10 (IL-10) in lipopolysaccharide (LPS) induced endotoxemia are not clear; their protective role is being investigated so that they may effectively modulate the host cytokine levels during endotoxemia. The aim of the study was to evaluate protective effects of IL-18 ...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2010-11-01 00:00:00
abstract：:Antiperoxidative property of N. jatamanasi was tested by using iron induced lipid peroxidation in 5% rat liver homogenate. The degree of peroxidation was quantitated by thiobarbituric acid reactive substance (TBARS) content. The extract provided protection against lipid peroxidation. The hexane fraction was more poten...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1996-11-01 00:00:00
abstract：:The Grey Weevil, Myllocerus subfasciatus Guerin, is an important emerging pest of quarantine significance in Solanaceae crops including the eggplant, Solanum melongena. Entomopathogenic nematodes (EPNs) have been shown to be a potential source of safe and effective control of M. subfasciatus. In this study, we determi...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2016-12-01 00:00:00
abstract：:Potentiality of C. tropicalis and D. polymorphus, to produce surface active compounds (bioemulsifiers/biosurfactants) during shake cultivation on hexadecane and oily waste was studied. Better emulsification activity, specific towards aromatic hydrocarbons, was observed with C. tropicalis culture broth. Emulsification ...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1989-03-01 00:00:00
abstract：:The antioxidant activities of the crude hydro-alcoholic extract (CE) and its four fractions viz. methanol (MF), ethyl acetate (EF), n-Butanol (BF), and precipitated aqueous (PAF) of A.racemosus roots tested decreased in the order of EF > MF > CE > BF > PAF when investigated by DPPH free radical scavenging assay. Under...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2012-11-01 00:00:00
abstract：:Substance P (SP) injection in the plantar region of rat hind paw caused a dose related inflammation, which reached a peak within 10 min of injection and declined after 60 min. Low doses (0.25-0.063 mg/kg) of SP-antagonists like (D-Pro2, D-Trp7,9)-SP and (D-Pro2, D-Phe7, D-Trp9)-SP pretreatment significantly inhibited ...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1990-10-01 00:00:00
abstract：:Bacopa monniera, Linn. (Brahmi: Scrophulariaceae) an Ayurvedic medicine is clinically used for memory enhancing, epilepsy, insomnia and as mild sedative. For the first time the effect of alcohol and hexane fraction of Brahmi has been studied on FeSO4 and cumene hydroperoxide induced lipid peroxidation. Alcohol fractio...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1996-06-01 00:00:00
