Abstract:
:The ELISA and GM1-ELISA, by using antiserum to purified Salmonella enterotoxin (SE), were standardized and carried out to screen salmonellae isolated from foods of animal origin for enterotoxigenicity. Of the 101 strains of Salmonella belonging to 15 different serogroups tested, 76 (75.24%) strains from 13 serogroups were found enterotoxigenic. ELISA correlated well with rabbit ligated ileal loop (RLIL) test for the detection of enterotoxin producing salmonellae with 24 test strains. ELISA also yielded positive reaction with 7 of 13 RLIL negative strains. GM1-ELISA could not be carried out as none of the 101 cell free culture supernatants (CFCS) were able to bind with GM1-ganglioside. ELISA and GM1-ELISA were also standardized with antiserum to cholera toxin for the detection of salmonellae producing cholera related enterotoxin. None of the 101 strains was found to produce cholera related enterotoxin. ELISA could detect as low as 15 ng/100 microliters of purified SE and 10 ng/100 microliters of cholera toxin when tested with their homologous antisera.
journal_name
Indian J Exp Bioljournal_title
Indian journal of experimental biologyauthors
Rahman H,Singh VB,Sharma VD,Singh SPsubject
Has Abstractpub_date
1991-10-01 00:00:00pages
982-4issue
10eissn
0019-5189issn
0975-1009journal_volume
29pub_type
杂志文章abstract::A new gamma-aminobutyric acid derivative, N-phthaloyl GABA (P-GABA), was synthesised and its anticonvulsant activity was tested and compared with sodium valproate for efficacy against experimentally induced convulsions in mice. At a dose of 80 mg/kg, P-GABA rendered more protection than sodium valproate. ED50 of P-GAB...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1989-09-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1996-08-01 00:00:00
abstract::Effects of short-term administration of corticosterone and cortisol on plasma levels of thyroid hormones, gonado-somatic index and testicular histology have been reported in catfish, Clarias gariepinus during different phases of its breeding cycle. Corticosterone administration had no significant effect on plasma leve...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2012-06-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2003-06-01 00:00:00
abstract::Iron deficiency during pregnancy and lactation (35 mg iron/kg diet) produced a significant reduction in liver nonheme iron in dams as well as in fetus and young ones. The body, liver and brain weights of fetus, new-born, and developing pups remained unaffected. However, the body weight and PCV were reduced only in 21-...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1990-05-01 00:00:00
abstract::Polypeptides synthesized from polysome-bound and unbound fractions of polyA mRNA were studied by in-vivo cells labelling and cDNA-mRNA hybrid-arrest translation. The polypeptides synthesized from unbound(free) fraction appeared to be significantly reduced in the absence of cycloheximide in L6 myoblast. The cDNA excess...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1997-09-01 00:00:00
abstract::Effects of pre- and post-natal undernutrition on learning and memory parameters were studied in albino rats. Prenatal undernutrition was induced in rat pups by restricting the mother's diet by 50% during the entire gestation period, whereas postnatal undernutrition was induced in rat pups by restriction of their diet ...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract::Multiple doses of H. spinosa were administered, ip once in a week for 4 weeks (20, 40 and 80 mg/kg body weight) and daily for one month (2, 4 and 8 mg/kg body weight). No alteration in biochemical parameters at low and moderate dose level of daily and low dose level of weekly treatment of petroleum ether extract was o...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1996-12-01 00:00:00
abstract::Absence of centrilobular necrosis, cirrhosis and giant hepatic cells with a large nucleus were significant observations in the liver of CCl4 treated rats following unilateral parathyroidectomy. Increased number of mitochondria and presence of binucleated cells indicated hepatic regeneration in unilaterally parathyroid...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1997-05-01 00:00:00
abstract::The whole plant aqueous extract of E. littorale was tested for its hypoglycemic activity on normoglycemic, hyperglycemic and alloxan induced diabetic rats. Blood sugar lowering activity was not observed in normoglycemic and glucose loaded hyperglycemic rats in the short time experiment. But in case of diabetic rats, t...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2000-08-01 00:00:00
abstract::A strain of T. chilonis, an egg parasitoid of lepidopteran pests tolerant to the most commonly used cyclodiene insecticide--endosulfan was developed in the laboratory. Tolerance to endosulfan was induced by exposing adult parasitoids sequentially from a sub-lethal concentration (0.004%) to the field recommended concen...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2006-07-01 00:00:00
abstract::Germ cell death and their removal from the seminiferous epithelium are common in the affected testis in conditions of unilateral ischemia or cryptorchidism; the similarities and differences, however, have not been studied between these two conditions. The present study was designed to examine the severity of the effec...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2005-11-01 00:00:00
abstract::Breast cancer is a common malignancy in women all over the world and novel therapeutic approaches are required for the treatment of patients who become refractory to conventional therapies. Thyroid cancer is being treated successfully with radioiodine since many years. The iodide is transported inside the thyroid epit...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2011-06-01 00:00:00
abstract::Aqueous extract of T. cordifolia inhibited Fenton (FeSO4) reaction and radiation mediated 2-deoxyribose degradation in a dose dependent fashion with an IC50 value of 700 microg/ml for both Fenton and radiation mediated 2-DR degradation. Similarly, it showed a moderate but dose dependent inhibition of chemically genera...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2002-06-01 00:00:00
abstract::Natural Killer activity assessed by 51Cr release assay from K-562 cells showed detectable activity from 5th day after tumour transplantation, reaching a peak on 12th day and thereafter showing a gradual decline in the activity. Antibody dependent cell mediated cytotoxicity estimated by 51Cr labelled sheep red blood ce...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1989-06-01 00:00:00
abstract::Goat epididymal and ejaculated spermatozoa were incubated in Krebs-Ringer bicarbonate buffer containing pyruvate and lactate as energy source. A 3 hr incubation for epididymal and 4 hr for ejaculated spermatozoa was required for the capacitation and acrosome reaction to take place. Calcium is an essential requirement ...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1989-11-01 00:00:00
abstract::Aluminium phosphide(AlP), a grain fumigant pesticide, was studied for its cardiotoxicity in anaesthetised rats. The hemodynamic and cardiac biochemical changes were investigated following intragastric administration of different doses of AlP (10, 20 and 40 mg). With 10 and 20 mg dose of AlP an immediate fall in BP was...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1997-10-01 00:00:00
abstract::The effects of dopamine (DA) agonists and antagonists were investigated on indomethacin--and restraint stress (6 hr at RT)--induced gastric ulcer formation in rats. The DA-agonists, apomorphine and bromocryptine (both at 5 mg/kg) significantly attenuated the frequency and severity of gastric mucosal lesions in both ex...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1990-06-01 00:00:00
abstract::The effect of the administration of three different concentrations (2.25, 1.13 and 0.56 mg/100 g body weight) of dimethoate on the urinary excretion of total, non dialysable and free hydroxyproline were studied in female albino rats. Compared to controls, the urine contents were significantly lower in higher concentra...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1992-06-01 00:00:00
abstract::A highly stable lipase from Pseudomonas aeruginosa KKA-5 was produced by batch cultivation technique employing shake flask and 5 L-bioreactor. The bioreactor was run at different airflow rates. Low airflow rates (1 and 3 L/min), did not lead to effective growth and lipase production. Growth increased by about one orde...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1999-05-01 00:00:00
abstract::A feasibility study was conducted to evaluate the capability of bacterial strains to degrade crude oil under in vitro conditions. Pseudomonas strain PS-I could degrade alkanes (70.69%) and aromatics (45.37%). Alkanes and aromatic fractions separated by column chromatography were analyzed by gas chromatography. In case...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2009-09-01 00:00:00
abstract::Urine samples, collected from Sprague Dawley rats treated with extracts of tobacco/masheri, benzo (a) pyrene, N'-nitrosonornicotine, N'-nitrosodiethylamine and maintained on semi-synthetic diets sufficient or deficient in Vitamin A, B and protein were tested for mutagenicity using Salmonella/microsome assay. The mutag...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1990-08-01 00:00:00
abstract::The in vitro percutaneous absorption of verapamil hydrochloride (VHCl) was investigated in order to assess its feasibility for transdermal development. The experiments were carried across mice and guinea pig skins using Keshary-Chien diffusion cell. The values of diffusion rate (J) and permeability coefficient (Kp) ac...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1996-05-01 00:00:00
abstract::Spirulina platensis treatment (400 mg kg(-1) for 25 days) effectively suppressed peripheral sensitization via modulation of glial activation and improved motor coordination and restoration of functional motor activity in collagen-induced arthritic rats. Spirulina treatment also resulted in an appreciable reduction of ...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2011-10-01 00:00:00
abstract::Various storage treatments on human blood samples have been described with respect to DNA yield, quality and fingerprinting. Blood samples were stored at room temperature, 4 degrees C, -20 degrees C and -70 degrees C for different duration varying from 1, 2, 4 and 8 weeks with or without anticoagulant/preservative (ED...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1996-04-01 00:00:00
abstract::Ricin, a glycoprotein from castor oil seeds, is specifically toxic to Kupffer cells and at low doses it leaves parenchymal cells comparatively unaffected. At a dose of approximately 1.5 microgram/100 g body weight, ricin significantly increases the hepatic antioxidant enzyme system in rats within 24 hr. Superoxide dis...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1994-05-01 00:00:00
abstract::Therapeutic efficacy of EGTA (ethylene glycol bis (2-aminoethyl ether) tetraacetic acid) against cerium intoxicated mice was studied. Administration of cerium showed significant decrease in haemoglobin percentage, RBC counts and blood glucose level with an increase in the activity of serum transaminases and WBC counts...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2004-09-01 00:00:00
abstract::Mouse B16LuF1 melanoma cells of lower metastatic potential to lung were treated in vitro with same concentration (50 microM) of gangliosides prepared from plasma of mice bearing lung metastasis of B16LuF5, B16LuF9 or B16LuF10 melanoma cell lines of increasing metastatic potential to lung (LuF1 < LuF5 < LuF9 < LuF10) a...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2004-10-01 00:00:00
abstract::The present investigation is aimed to justify the validity of traditional practice of using the frog skin in wound healing. Studies were carried out on skin wounds from female albino rats of Wistar strain weighing 100-120 g. The wounds of standard size (5 cm2) were created in the experimental and control groups. The e...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1995-09-01 00:00:00
abstract::Geminiviruses are single-stranded DNA plant infecting viruses that cause major losses in important crops in tropical and subtropical countries. Tomato leaf curl virus (TLCV) belonging to the genera Begomovirus, is a whitefly-transmitted geminivirus that causes a severe leaf curl disease in tomato (Lycopersicon esculen...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2000-05-01 00:00:00