Differential toxicological endpoints of di(2-ethylhexyl) phthalate (DEHP) exposure in MCF-7 and MDA-MB-231 cell lines: possible estrogen receptor alpha (ERalpha) independent modulations.

Abstract:

:Wide spread use of Di-(2-ethylhexyl) phthalate (DEHP) has made it a ubiquitous contaminant in today's environment, responsible for possible carcinogenic and endocrine disrupting effects. In the present investigation an integrative toxicoproteomic approach was made to study the estrogenic potential of DEHP. In vitro experiments carried out with DEHP (0.1-100 microM) induced proliferations (E-screen assay) in human estrogen receptors-alpha (ERalpha) positive MCF-7 and ERalpha negative MDA-MB-231 breast cancer cells irrespective of their ERa status. Further, DEHP suppressed tamoxifen (a potent anti-breast cancer drug) induced apoptosis in both cell types as shown by flowcytometric cell cycle analysis. Label-free quantitative proteomics analysis of the cell secretome of both the cell lines indicated a wide array of stress related, structural and receptor binding proteins that were affected due to DEHP exposure. The secretome of DEHP treated MCF-7 cells revealed the down regulation of lactotransferrin, an ERalpha responsive iron transport protein. The results indicated that toxicological effects of DEHP did not follow an ERa signaling pathway. However, the differential effects in MCF-7 and MDA-MB-231 cell lines indicate that ERa might have an indirect modulating effect on DEHP induced toxicity.

journal_name

Indian J Exp Biol

authors

Tanay Das M,Kumar M,Thakur IS

subject

Has Abstract

pub_date

2014-11-01 00:00:00

pages

1052-61

issue

11

eissn

0019-5189

issn

0975-1009

journal_volume

52

pub_type

杂志文章
  • 5-HT3 receptors in selective animal models of cognition.

    abstract::Role of 5-HT3 receptors in cholinergic hypofunctional models of cognitive impairment in the elevated plus maze model and a passive avoidance model is studied. Cognitive impairment was caused by scopolamine (1 mg/kg, ip) in mice and 5-HT3 ligands mCPBG (1 and 5 mg/kg, ip) and ondansetron (0.5 and 5 mg/kg, ip) were admi...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Sridhar N,Veeranjaneyulu A,Arulmozhi DK,Gupta CN,Babu RJ

    更新日期:2002-02-01 00:00:00

  • Immunosuppression due to chronic Lantana camara, L. toxicity in sheep.

    abstract::Five sheep were intoxicated with powdered L. camara, leaves at the rate of 200 mg/kg body weight daily for 110 days and another group of four sheep were drenched with distilled water daily for equal number of days which acted as control. The cell mediated immunity of intoxicated and control sheep was assessed with the...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Ganai GN,Jha GJ

    更新日期:1991-08-01 00:00:00

  • Oral toxicity of Madhuca butyracea Macb. saponins to albino rats.

    abstract::Saponins, isolated from M. butyracea, were assessed for their acute and subacute oral toxicity in albino rats. Acute doses of saponins caused mortalities and LD50 and LD90 values were 330 and 430 mg/kg body wt respectively. Severe diarrhoea, restlessness and histopathological changes were observed in liver and kidney....

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Lalitha T,Vishwanatha S,Venkataraman LV

    更新日期:1990-07-01 00:00:00

  • Indirect excitatory actions of histamine on isolated chicken oesophagus.

    abstract::The histamine-induced contraction on chicken oesophagus was antagonised non-competitively by atropine, hexamethonium, cocaine, methysergide, indomethacin, theophylline and verapamil. Physostigmine slightly potentiated the excitatory action of histamine. These results indicate that histamine excreted its excitatory act...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Kalra AK,Verma SP,Garg SK,Uppal RP

    更新日期:1993-12-01 00:00:00

  • Effect of lead with vitamin E, C, or Spirulina on malondialdehyde, conjugated dienes and hydroperoxides in rats.

    abstract::Lead (100 ppm) was given in doubly deionised water for 30 days to one group of rats. The other groups received lead along with exogenous antioxidants like vitamin E (50 IU/kg), vitamin C (800 mg/kg) or Spirulina (1500 mg/kg) in food for a similar period. Levels of lipid peroxidation products such as malondialdehyde, c...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Upasani CD,Khera A,Balaraman R

    更新日期:2001-01-01 00:00:00

  • Wheat (Triticum aestivum) peptide (s) mimic gibberellin action and regulate stomatal opening.

    abstract::Wheat peptides (0.5 to 3 KDa M(r)) mimick hormonal activity like that of gibberellins and forced open dark closed stomata. The deionized amphoteric peptides solution after passing through cation and anion exchanger resins was run through Amicon's ultrafilters, 10, 3 and 0.5 kDa (M(r)) cut off system. The 3 to 0.5 kDa ...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Ghosh A,Mandal P,Sircar PK

    更新日期:2010-01-01 00:00:00

  • Effects of silymarin on UDP-glucuronic acid and glucuronidation activity in the rat isolated hepatocytes and liver in relation to D-galactosamine toxicity.

    abstract::Influence of silymarin on UDP-glucuronic acid (UDPGA) and glucuronidation activity of freshly isolated rat hepatocytes in suspension and in rat liver in vivo was examined. Viability of the hepatocytes (> 85%) was not altered in Hank's balanced salt solution at least for 4 hr at 37 degrees C under oxygen. Silymarin at ...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Chrungoo VJ,Reen RK,Singh K,Singh J

    更新日期:1997-03-01 00:00:00

  • Secretion of ligninperoxidase by Penicillium citrinum, Fusarium oxysporum and Aspergillus terreus.

    abstract::Secretion of ligninperoxidase [E.C.1.11.1.7] by Penicillium citrinum, Fusarium oxysporum and Aspergillus terreus in liquid culture growth medium has been demonstrated. Enzymatic characteristics like Km, pH and temperature optima using veratryl alcohol as the organic substrate of ligninperoxidases from above sources ha...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Kumari M,Yadav RS,Yadav KD

    更新日期:2002-07-01 00:00:00

  • Cellular and molecular determinants of ageing.

    abstract::The highly complex nature of the process of ageing implicates both genetic and epigenetic causative factors. A progressive failure of maintenance underlines and typifies this process. The instability of the nuclear and the mitochondrial genomes is an important determinant of ageing. Infidelity and misregulation of gen...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章,评审

    doi:

    authors: Rattan SI

    更新日期:1996-01-01 00:00:00

  • Differential C-heterochromatin distribution in two species of freshwater fish, Anabas testudineus (Bloch.) and Puntius sarana (Hamilton.).

    abstract::Anabas testudineus (2n = 46) had the more conserved pattern of its C-heterochromatin distributed mainly in the centromeric region, whereas Puntius sarana (2n = 50) exhibited a rather unorthodox pattern, many chromosomes showing interstitial, some telomeric and a few chromosomes showing centromeric C-band localization....

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Khuda-Bukhsh AR,Chakrabarti C

    更新日期:2000-03-01 00:00:00

  • Interrelationship between amines level in brain regions of fish, Oreochromis mossambicus during organophosphorus stress.

    abstract::Amines such as dopamine, norepinephrine and epinephrine were analysed in the brain regions of O.mossambicus exposed to quinalphos, phenthoate and their combination for 96 hr. The three types of treatments significantly (P < 0.05) altered the amines level at various intervals in the brain regions. ...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Durairaj S,Selvarajan VR,Devaraj P

    更新日期:1997-06-01 00:00:00

  • Low cost single-step purification of endoglucanase from Aspergillus fumigatus ABK-9.

    abstract::Low cost agro-waste was used as adsorption support for single-step purification of endoglucanase from the culture filtrate of A. fumigatus ABK-9. Among various agro-waste substrates, 1% NaOH pretreated rice bran was proved to be the best for adsorbing about 74.8 and 71.1% of endoglucanase at 4 degrees C and 10 degrees...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Das A,Paul T,Halder SK,Jana A,Ghosh K,Maity C,Das Mohapatra PK,Pati BR,Mondal KC

    更新日期:2013-11-01 00:00:00

  • Antihyperglycemic, antihyperlipidemic and antioxidant effects of Dihar, a polyherbal ayurvedic formulation in streptozotocin induced diabetic rats.

    abstract::Present investigation was undertaken to evaluate antihyperglycemic, antihyperlipidemic and antioxidant activities of Dihar, a polyherbal formulation containing drugs from eight different herbs viz., Syzygium cumini, Momordica charantia, Emblica officinalis, Gymnema sylvestre, Enicostemma littorale, Azadirachta indica,...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Patel SS,Shah RS,Goyal RK

    更新日期:2009-07-01 00:00:00

  • X-ray microanalysis of elements of VA mycorrhizal and non-mycorrhizal Pennisetum pedicellatum roots.

    abstract::Chemical composition of inter and intracellular hyphae was elucidated in Pennisetum pedicellatum roots colonized by Gigaspora margarita by employing energy dispersive X-ray microanalysis. Maximum amount of phosphate was estimated in polyphosphate granules present in the roots. Amount of phosphate was low in fungal hyp...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Ramesh C,Chellappan P,Mahadevan A

    更新日期:2000-04-01 00:00:00

  • Comparative analysis of protein profiles of wild virulent (E156) and aroA-htrA double deletion mutant vaccine strain (S30) of Salmonella enterica subsp. enterica serovar Abortusequi under in vivo and in vitro growth conditions.

    abstract::In the present study, cell lysate and cell supernatant of the both strains i.e., virulent wild type (E156) and mutant (S30) vaccine strains of Salmonella enterica subspecies enterica serovar Abortusequi (S. Abortusequi), grown under varied in vivo and in vitro conditions were subjected to SDS PAGE and western blotting...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Chandra M,Singh BR,Srivastava SK,Chaudhry P,Agrawal RK,Sharma A

    更新日期:2008-09-01 00:00:00

  • Effect of gossypol on few testicular enzymes in mature rats.

    abstract::Effect of oral administration of gossypol acetic acid (15 mg/kg/day) for 10 weeks, on certain enzymes, which may be taken as markers for the different stages of spermatogenesis, was studied in male albino rats. Gossypol produced a significant decrease in hyaluronidase and sorbitol dehydrogenase, while no change was ob...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Nair IN,Bhiwgade DA

    更新日期:1989-12-01 00:00:00

  • The circadian organization of the cardiovascular system in health and disease.

    abstract::In normal conditions, the temporal organization of blood pressure (BP) is mainly controlled by neuroendocrine mechanisms. Above all, the monoaminergic systems (including variations in activity of the autonomous nervous system, and in secretion of biogenic amines) appear to integrate the major driving factors of tempor...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章,评审

    doi:

    authors: Portaluppi F

    更新日期:2014-05-01 00:00:00

  • Antiulcerogenic activity of Satavari mandur--an Ayurvedic herbo-mineral preparation.

    abstract::Satavari mandur (SM) is a herbo-mineral preparation containing Asparagus racemosus, which finds mention in ancient Indian texts for treatment of gastric ulcers. The ulcer protective effect of SM, 125-500 mg/kg given orally, twice daily for three, five and seven days, was studied on cold restraint stress-induced gastri...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Datta GK,Sairam K,Priyambada S,Debnath PK,Goel RK

    更新日期:2002-10-01 00:00:00

  • Interaction of T-DNA border sequences and Ti-plasmid vir functions of Agrobacterium results in differential single-stranded linear T-DNA molecule production and plant transformation.

    abstract::The mechanism of Agrobacterium mediated genetic transformation of plants is dependent upon certain genetic function of the chromosome of the bacterium as well as on Ti-plasmid borne vir loci and the border sequences of T-DNA. The organisationally variable forms of the naturally occurring border sequences amongst Ti-pl...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Das S,Basu D,Mukherjee P,Sen SK

    更新日期:1991-11-01 00:00:00

  • Effect of chronic ethanol administration on testicular antioxidant system and steroidogenic enzyme activity in rats.

    abstract::In order to find out the effect of chronic ethanol administration on testicular antioxidant system and steroidogenic enzyme activity, male rats fed with ethanol 1.6g/kg body weight per day for four weeks were studied. Besides a drastic reduction in body and testis weight, there was decrease in ascorbic acid, reduced g...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Maneesh M,Jayalekshmi H,Dutta S,Chakrabarti A,Vasudevan DM

    更新日期:2005-05-01 00:00:00

  • A method for large-scale multiplication of Curculigo orchioides through bulbil formation from leaf explant in shake flask culture.

    abstract::An efficient method has been developed for large-scale multiplication of Curculigo orchioides (Hypoxidaceae), an endangered medicinal plant, through direct bulbil formation from leaf explants in shake flask cultures. Leaf-segments (7 x 10 mm) were cultured in B5 liquid medium containing KNO3 (200 mgNL-1), (NH4)2SO4 (5...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Suri SS,Arora DK,Ramawat KG

    更新日期:2000-02-01 00:00:00

  • A novel assay method for calcium calmodulin dependent phosphatase from bovine brain extract.

    abstract::Calcium calmodulin dependent protein ser/thr phosphatase, also referred to as protein phosphatase 2B (PP2B), is rich in neural tissue, and plays an important role in the overall function of the nervous system. Routinely phosphatase assay employs, para-Nitrophenlylphosphate (p-NPP), as a substrate, is also extended to ...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Devaraju KS,Kumar BS,Sureshbabu SV,Gopi A,Saraswathi R,Harish BM

    更新日期:2014-02-01 00:00:00

  • Hepato and reno protective action of Calendula officinalis L. flower extract.

    abstract::Flower extract of C. officinalis L. was evaluated for its protective effect against CCl4 induced acute hepatotoxicity and cisplatin induced nephrotoxicity. The activities of serum marker enzymes of liver injury like glutamate pyruvate transaminase (SGPT), glutamate oxaloacetate transaminase (SGOT) and alkaline phospha...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Preethi KC,Kuttan R

    更新日期:2009-03-01 00:00:00

  • Secretion of metastasis related gangliosides by mouse B16-melanoma in circulation in vivo and in culture media in vitro.

    abstract::Mouse B16LuF1 melanoma cells of lower metastatic potential to lung were treated in vitro with same concentration (50 microM) of gangliosides prepared from plasma of mice bearing lung metastasis of B16LuF5, B16LuF9 or B16LuF10 melanoma cell lines of increasing metastatic potential to lung (LuF1 < LuF5 < LuF9 < LuF10) a...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Saha S,Mohanty KC

    更新日期:2004-10-01 00:00:00

  • Modulation of gamma glutamyl transpeptidase activity in lymphoid system by organochlorine pesticides in rats.

    abstract::Effects of 1,1,1,-trichloro-2,2-bis (p-chlorophenyl) ethane (DDT) and lindane were studied on gamma glutamyl transpeptidase (GGT) activity in different tissues of the lymphoid system in rats. DDT (100 or 200 ppm) and lindane (30 or 80 ppm) exposure for 8 weeks suppressed the GGT activity in a dose dependent manner in ...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Koner BC,Banerjee BD,Ray A

    更新日期:1997-10-01 00:00:00

  • Testicular toxicity of di-n-butyl phthalate in adult rats: effect on marker enzymes of spermatogenesis.

    abstract::Di-n-butyl phthalate (DBP) was administered to adult male rats by gavage at the doses of 250, 500 and 1000 mg/kg body weight/day for 15 days. A significant decrease in epididymal spermatozoa counts was observed at 500 and 1000 mg/kg doses of DBP. The activity of sorbitol dehydrogenase was found to be significantly dec...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Srivastava S,Singh GB,Srivastava SP,Seth PK

    更新日期:1990-01-01 00:00:00

  • Swerchirin induced blood sugar lowering of streptozotocin treated hyperglycemic rats.

    abstract::Effect of Swerchirin (1:8 dihydroxy 3:5 dimethoxy xanthone) isolated from hexane fraction of Swertia chirayita on the blood sugar level of healthy and streptozotocin treated rats was studied. Streptozotocin was administered in citrate buffer (pH-4.5) intravenously / 35 and 65 mg/kg body wt to Charles Foster strain alb...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Saxena AM,Bajpai MB,Mukherjee SK

    更新日期:1991-07-01 00:00:00

  • Occurrence of sopE gene and its phenotypic expression among different serovars of Salmonella enterica isolated from man and animals.

    abstract::Salmonella pathogenesis is a complex phenomenon and a Type III secretion system plays a central role in the development of Salmonella-induced enteritis. One such Type III secretion protein is Salmonella outer protein E (SopE). Prevalence of sopE gene and its phenotypic expression (SopE protein) among different serovar...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Rahman H,Hardt WD,Murugkar HV,Bhattacharyya DK

    更新日期:2005-07-01 00:00:00

  • Precocious recrudescence of seminal vesicle and testis in catfish, Clarias batrachus (Linn.), subjected to a long photoperiod regime.

    abstract::The catfish C. batrachus were exposed to a long photoperiod of 14 hr light during resting--early preparatory (December-February) phases of the reproductive cycle. At 70-day sampling, both the seminal vesicle (SV) and testis registered marked stimulatory effects in comparisons to control fish maintained under approxima...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Singh MS,Joy KP

    更新日期:1998-12-01 00:00:00

  • Concentration of cations in different parts of male reproductive system and their influence on in vitro sperm motility in lizard, Mabuya carinata Schneider.

    abstract::Concentrations of mono- and divalent cations in the testis, three different regions of the epididymis and the vas deferens of lizard, M. carinata were determined. The concentrations of Na+, K+ and Ca2+ increased gradually from testis to vas deferens. However, no significant variation in the levels of Mg2+ was observed...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Aranha I,Bhagya M,Yajurvedi HN

    更新日期:2008-10-01 00:00:00