Ductal epithelial cells cultured from human foetal epididymis and vas deferens: relevance to sterility in cystic fibrosis.

abstract：:Cell-free extracts of Xenopus eggs support nuclear assembly and DNA replication in vitro. Extracts supplemented with the protein phosphatase inhibitor microcystin-LR displayed various inhibitory effects at different concentrations of the toxin. In the presence of cycloheximide, additions of microcystin did not induce ...

journal_title：Journal of cell science

authors： Murphy J,Crompton CM,Hainey S,Codd GA,Hutchison CJ

更新日期：1995-01-01 00:00:00

abstract：:Cyclic AMP-dependent protein kinase (cAPK) is the intracellular mediator of signal transduction events involving the adenylate cyclase-cyclic AMP system. A monoclonal antibody (MAb BB1) to the type II regulatory subunit (RII) of cAPK was used in a post-embedding immunogold-labeling procedure to determine the ultrastru...

journal_title：Journal of cell science

authors： Hand AR,Mednieks MI

更新日期：1989-08-01 00:00:00

abstract：:Cell surface glycans are believed to play a role in tumour invasion and metastasis. Yet, we have previously shown that the inhibitors of N-linked glycan processing swainsonine (SW) and 1-deoxynojirimycin (dNM) did not prevent invasion of chick heart fragments by MO4 murine fibrosarcoma cells in organ culture. We now p...

journal_title：Journal of cell science

authors： Bruyneel EA,De Mets M,Dragonetti CH,Hooghe RJ,Di Virgilio S,Mareel MM

更新日期：1990-02-01 00:00:00

abstract：:A monoclonal antibody, specific to phosphoproteins in mitotic HeLa cells was found to crossreact with a similar set of proteins in embryos of the nematode, Caenorhabditis elegans. In C. elegans, as in mammalian cells, the highly conserved antigenic epitope is associated with a family of high molecular weight polypepti...

journal_title：Journal of cell science

authors： Hecht RM,Berg-Zabelshansky M,Rao PN,Davis FM

更新日期：1987-03-01 00:00:00

abstract：:It remains unclear how GPI-anchored proteins (GPIAPs), which lack cytoplasmic domains, transduce signals triggered by specific ligation. Such signal transduction has been speculated to require the ligated GPIAP to associate with membrane-spanning proteins that communicate with obligate cytoplasmic proteins. Transient ...

journal_title：Journal of cell science

authors： Chen Y,Veracini L,Benistant C,Jacobson K

更新日期：2009-11-01 00:00:00

abstract：:Rabbit muscle myosin subfragment S1 decorates 6 nm diameter filaments in Drosophila wing epidermal cells in the arrowhead fashion characteristic of the binding of subfragment S1 to actin filaments. The filaments in question are concentrated between microtubules that are mostly composed of 15 protofilaments and form ce...

journal_title：Journal of cell science

authors： Mogensen MM,Tucker JB

更新日期：1988-11-01 00:00:00

abstract：:Inactivation of the transcription factor and tumor suppressor p53, and overexpression or mutational activation of PIK3CA, which encodes the p110alpha catalytic subunit of phosphatidylinositol-3-kinase (PI3K), are two of the most common deleterious genomic changes in cancer, including in ovarian carcinomas. We investig...

journal_title：Journal of cell science

authors： Astanehe A,Arenillas D,Wasserman WW,Leung PC,Dunn SE,Davies BR,Mills GB,Auersperg N

更新日期：2008-03-01 00:00:00

abstract：:Caveolins are membrane proteins that are the major coat proteins of caveolae, specialized lipid rafts in the plasma membrane that serve as scaffolding sites for many signaling complexes. Among the many signaling molecules associated with caveolins are the Src tyrosine kinases, whose activation regulates numerous cellu...

journal_title：Journal of cell science

authors： Smythe GM,Eby JC,Disatnik MH,Rando TA

更新日期：2003-12-01 00:00:00

abstract：:Human cytomegalovirus (HCMV) establishes life-long latent infection in hematopoietic progenitor cells and circulating monocytes in infected individuals. Myeloid differentiation coupled with immune dysregulation leads to viral reactivation, which can cause severe disease and mortality. Reactivation of latent virus requ...

journal_title：Journal of cell science

authors： Krishna BA,Wass AB,Dooley AL,O'Connor CM

更新日期：2021-01-25 00:00:00

abstract：:ADP-ribosylation factors (Arfs) and ADP-ribosylation factor-like proteins (Arls) are highly conserved small GTPases that function as main regulators of vesicular trafficking and cytoskeletal reorganization. Arl1, the first identified member of the large Arl family, is an important regulator of Golgi complex structure ...

journal_title：Journal of cell science

authors： Yu CJ,Lee FJ

更新日期：2017-05-15 00:00:00

abstract：:BMP2 stimulates bone formation and signals preferably through BMP receptor (BMPR) 1A, whereas GDF5 is a cartilage inducer and signals preferably through BMPR1B. Consequently, BMPR1A and BMPR1B are believed to be involved in bone and cartilage formation, respectively. However, their function is not yet fully clarified....

journal_title：Journal of cell science

authors： Mang T,Kleinschmidt-Doerr K,Ploeger F,Schoenemann A,Lindemann S,Gigout A

更新日期：2020-08-21 00:00:00

abstract：:Endocytosis and recycling of membrane proteins are key processes for nutrient uptake, receptor signaling and synaptic transmission. Different steps in these fission and fusion cycles have been proposed to be regulated by physiological changes in plasma membrane (PM) phosphatidylinositol (4,5)-bisphosphate [PtdIns(4,5)...

journal_title：Journal of cell science

authors： Abe N,Inoue T,Galvez T,Klein L,Meyer T

更新日期：2008-05-01 00:00:00

abstract：:Little is known of the molecular basis for the diversity of microtubule structure and function found within the eukaryotic flagellum. Antibodies that discriminate between tyrosinated alpha tubulin and post-translationally detyrosinated alpha tubulin were used to localize these complementary tubulin isoforms in flagell...

journal_title：Journal of cell science

authors： Johnson KA

更新日期：1998-02-01 00:00:00

abstract：:The attachment of polymorphonuclear leukocytes (PMN) to the endothelial lining of blood vessels is an important initial event in the acute inflammatory reaction. Experiments were carried out to examine some of the parameters of this adhesive interaction and the extent to which the substrate (endothelium) plays a role ...

journal_title：Journal of cell science

authors： Hoover RL,Folger R,Haering WA,Ware BR,Karnovsky MJ

更新日期：1980-10-01 00:00:00

abstract：:Shape changes have been determined in human blood lymphocytes stimulated with OAG, diC8, PMA, colchicine or the hexapeptide fNLPNTL in short-term assays (30 min). Distinct types of shape-change responses were observed. Colchicine was active in generating a relatively small proportion of polarized lymphocytes (front-ta...

journal_title：Journal of cell science

authors： Keller HU,Niggli V,Zimmermann A

更新日期：1989-07-01 00:00:00

abstract：:Integrin-mediated cell adhesion is central to cell survival, differentiation and motility. Many cell responses induced by integrins require both receptor occupancy and receptor aggregation, and appear to be regulated by both biochemical and biophysical means. Multidomain extracellular matrix molecules may serve to fos...

journal_title：Journal of cell science

authors： Koo LY,Irvine DJ,Mayes AM,Lauffenburger DA,Griffith LG

更新日期：2002-04-01 00:00:00

abstract：:The cell surface delivery of extracellular matrix (ECM) and integrins is fundamental for cell migration in wound healing and during cancer cell metastasis. This process is not only driven by several soluble NSF attachment protein (SNAP) receptor (SNARE) proteins, which are key players in vesicle transport at the cell ...

journal_title：Journal of cell science

authors： Enrich C,Rentero C,Hierro A,Grewal T

更新日期：2015-03-15 00:00:00

abstract：:The human Ras superfamily of small GTPases controls essential cellular processes such as gene expression and cell proliferation. As their deregulation is widely associated with human cancer, small GTPases and their regulatory proteins have become increasingly attractive for the development of novel therapeutics. Class...

journal_title：Journal of cell science

authors： Koraïchi F,Gence R,Bouchenot C,Grosjean S,Lajoie-Mazenc I,Favre G,Cabantous S

更新日期：2018-01-08 00:00:00

abstract：:The constant shedding and renewal of epithelial cells maintain the protection of epithelial barriers. Interference with the processes of host cell-cycle regulation and barrier integrity permits the bacterial pathogen Neisseria gonorrhoeae to effectively colonize and invade epithelial cells. Here, we show that a gonoco...

journal_title：Journal of cell science

authors： Vielfort K,Söderholm N,Weyler L,Vare D,Löfmark S,Aro H

更新日期：2013-01-01 00:00:00

abstract：:Of the classical compartments of eukaryotic cells, peroxisomes were the last to be discovered. They are small, single-membrane-bound vesicles involved in cellular metabolism, most notably the beta-oxidation of fatty acids. Characterization of their properties and behavior has progressed rather slowly. However, during ...

journal_title：Journal of cell science

authors： van der Zand A,Braakman I,Geuze HJ,Tabak HF

更新日期：2006-03-15 00:00:00

abstract：:Endothelial integrity relies on a mechanical crosstalk between intercellular and cell-matrix interactions. This crosstalk is compromised in hemorrhagic vascular lesions of patients carrying loss-of-function mutations in cerebral cavernous malformation (CCM) genes. RhoA/ROCK-dependent cytoskeletal remodeling is central...

journal_title：Journal of cell science

authors： Lisowska J,Rödel CJ,Manet S,Miroshnikova YA,Boyault C,Planus E,De Mets R,Lee HH,Destaing O,Mertani H,Boulday G,Tournier-Lasserve E,Balland M,Abdelilah-Seyfried S,Albiges-Rizo C,Faurobert E

更新日期：2018-08-13 00:00:00

abstract：:A key step in cell migration is the dynamic formation and disassembly of adhesions at the front and the concomitant movement and release of adhesions in the rear of the cell. Fibroblasts maintained in the absence of serum have stable adhesions within the rear of the cell and exhibit reduced trailing-edge retraction re...

journal_title：Journal of cell science

authors： Iwanicki MP,Vomastek T,Tilghman RW,Martin KH,Banerjee J,Wedegaertner PB,Parsons JT

更新日期：2008-03-15 00:00:00

abstract：:In hydra and in mammalian cells the monoclonal antibody V recognises an epitope which colocalises with cytoskeletal structures. Using this antibody for expression screening, a cDNA clone (955 bp) was isolated from hydra, which covers an open reading frame for a protein of 294 amino acids with a calculated molecular ma...

journal_title：Journal of cell science

authors： Keppel E,Schaller HC

更新日期：1991-12-01 00:00:00

abstract：:The adaptive immune response depends on the interaction of T cells and antigen-presenting cells at the immune synapse. Formation of the immune synapse and the subsequent T-cell activation are highly dependent on the actin cytoskeleton. In this work, we describe that T cells express drebrin, a neuronal actin-binding pr...

journal_title：Journal of cell science

authors： Pérez-Martínez M,Gordón-Alonso M,Cabrero JR,Barrero-Villar M,Rey M,Mittelbrunn M,Lamana A,Morlino G,Calabia C,Yamazaki H,Shirao T,Vázquez J,González-Amaro R,Veiga E,Sánchez-Madrid F

更新日期：2010-04-01 00:00:00

abstract：:In eukaryotes, mRNA export involves many evolutionarily conserved factors that carry the nascent transcript to the nuclear pore complex (NPC). The THO/TREX complex couples transcription to mRNA export and recruits the mRNA export receptor NXF1 for the transport of messenger ribonucleoprotein particles (mRNP) to the NP...

journal_title：Journal of cell science

authors： Umlauf D,Bonnet J,Waharte F,Fournier M,Stierle M,Fischer B,Brino L,Devys D,Tora L

更新日期：2013-06-15 00:00:00

abstract：:Pex11 homologs and dynamin-related proteins uniquely regulate peroxisome division (cell-cycle-dependent duplication) and proliferation (cell-cycle-independent multiplication). Arabidopsis plants possess five Pex11 homologs designated in this study as AtPex11a, -b, -c, -d and -e. Transcripts for four isoforms were foun...

journal_title：Journal of cell science

authors： Lingard MJ,Trelease RN

更新日期：2006-05-01 00:00:00

abstract：:We investigated mechanisms of the p73alpha-mediated repression of the platelet-derived growth factor beta-receptor (PDGFRB) promoter caused by its interaction with NF-Y. Treatment of cells with the histone deacetylase (HDAC) inhibitor, Trichostatin A, increases PDGFRB promoter activity through the CCAAT motif and coun...

journal_title：Journal of cell science

authors： Uramoto H,Wetterskog D,Hackzell A,Matsumoto Y,Funa K

更新日期：2004-10-15 00:00:00

abstract：:Quantitative experimental data from studies of intracellular processing and transport of secretory and membrane proteins are only rarely evaluated using kinetic modelling. Instead, the analysis of such data is usually limited to calculating either the apparent half-life or the transit time. Neither of these parameters...

journal_title：Journal of cell science

authors： Noe DA,Delenick JC

更新日期：1989-03-01 00:00:00

abstract：:To evaluate the relative mobilities of cell surface glycoconjugates during myogenesis we have studied the redistribution of fluorescein-conjugated plant lectins on L6 rat myogenic cells. Previous experiments had demonstrated that the receptors for the lectins soybean agglutinin (SBA), wheat germ agglutinin, concanaval...

journal_title：Journal of cell science

authors： Sawyer JT,Akeson RA

更新日期：1986-07-01 00:00:00

abstract：:Cytokines, such as interferons, erythropoietin, leptin and most interleukins, signal through type 1 cytokine receptors and activate the canonical JAK-STAT pathway. Aberrant cytokine signalling underlies numerous pathologies and adequate, temporary receptor activation is therefore under tight control. Negative-feedback...

journal_title：Journal of cell science

authors： Wauman J,De Ceuninck L,Vanderroost N,Lievens S,Tavernier J

更新日期：2011-03-15 00:00:00