Abstract：
:Over the last decades, great strides were made in the development of novel implants for the treatment of bone defects. The increasing versatility and complexity of these implant designs request for concurrent advances in means to assess in vivo the course of induced bone formation in preclinical models. Since its discovery, micro-computed tomography (micro-CT) has excelled as powerful high-resolution technique for non-invasive assessment of newly formed bone tissue. However, micro-CT fails to provide spatiotemporal information on biological processes ongoing during bone regeneration. Conversely, due to the versatile applicability and cost-effectiveness, single photon emission computed tomography (SPECT) would be an ideal technique for assessing such biological processes with high sensitivity and for nuclear imaging comparably high resolution (<1 mm). Herein, we employ modular designed poly(ethylene glycol)-based hydrogels that release bone morphogenetic protein to guide the healing of critical sized calvarial bone defects. By combined in vivo longitudinal multi-pinhole SPECT and micro-CT evaluations we determine the spatiotemporal course of bone formation and remodeling within this synthetic hydrogel implant. End point evaluations by high resolution micro-CT and histological evaluation confirm the value of this approach to follow and optimize bone-inducing biomaterials.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Lienemann PS,Metzger S,Kiveliö AS,Blanc A,Papageorgiou P,Astolfo A,Pinzer BR,Cinelli P,Weber FE,Schibli R,Béhé M,Ehrbar Mdoi
10.1038/srep10238subject
Has Abstractpub_date
2015-05-19 00:00:00pages
10238issn
2045-2322pii
srep10238journal_volume
5pub_type
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