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Cell-free DNA in the urine of rats exposed to ionizing radiation.

Abstract：

:Investigation of cell-free DNA (cf-DNA) in body fluids, as a potential biomarker for assessing the effect of ionizing radiation on the organism, is of considerable interest. We investigated changes in the contents of cell-free mitochondrial DNA (cf-mtDNA) and cell-free nuclear DNA (cf-nDNA) in the urine of X-ray-exposed rats. Assays of cf-mtDNA and cf-nDNA were performed by a real-time PCR in rat urine collected before and after irradiation of animals with doses of 3 and 5 Gy. We also determined the presence of mutations in urine cf-mtDNA, as recognized by Surveyor nuclease. A sharp increase in cf-mtDNA and cf-nDNA in the urine of irradiated rats was observed within 24 h after exposure, followed by a decrease to normal levels. In all cases, the contents of cf-mtDNA fragment copies (estimated by gene tRNA) were significantly higher than those of cf-nDNA estimated by gene GAPDH. A certain portion of mutant cf-mtDNA fragments was detected in the urine of exposed rats, whereas they were absent in the urine of the same animals before irradiation. These preliminary data also suggest that the increased levels of urine cf-mtDNA and cf-nDNA may be a potential biomarker for noninvasive assessment of how the organism responds to ionizing radiation influence.

journal_name

Radiat Environ Biophys

journal_title

Radiation and environmental biophysics

authors

Abdullaev SA,Minkabirova GM,Bezlepkin VG,Gaziev AI

doi

10.1007/s00411-015-0599-0

subject

Has Abstract

pub_date

2015-08-01 00:00:00

pages

297-304

issue

3

eissn

0301-634X

issn

1432-2099

journal_volume

54

pub_type

杂志文章

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