Abstract:
:In plants, Potato spindle tuber viroid (PSTVd) replication triggers post-transcriptional gene silencing (PTGS) and RNA-directed DNA methylation (RdDM) of homologous RNA and DNA sequences, respectively. PTGS predominantly occurs in the cytoplasm, but nuclear PTGS has been also reported. In this study, we investigated whether the nuclear replicating PSTVd is able to trigger nuclear PTGS. Transgenic tobacco plants carrying cytoplasmic and nuclear PTGS sensor constructs were PSTVd-infected resulting in the generation of abundant PSTVd-derived small interfering RNAs (vd-siRNAs). Northern blot analysis revealed that, in contrast to the cytoplasmic sensor, the nuclear sensor transcript was not targeted for RNA degradation. Bisulfite sequencing analysis showed that the nuclear PTGS sensor transgene was efficiently targeted for RdDM. Our data suggest that PSTVd fails to trigger nuclear PTGS, and that RdDM and nuclear PTGS are not necessarily coupled.
journal_name
RNA Bioljournal_title
RNA biologyauthors
Dalakouras A,Dadami E,Bassler A,Zwiebel M,Krczal G,Wassenegger Mdoi
10.1080/15476286.2015.1017216subject
Has Abstractpub_date
2015-01-01 00:00:00pages
268-75issue
3eissn
1547-6286issn
1555-8584journal_volume
12pub_type
杂志文章相关文献
RNA Biology文献大全abstract::Sin Nombre hantavirus (SNV) is a New World hantavirus and causes hantavirus cardiopulmonary syndrome. The viral nucleocapsid protein (N) is an RNA chaperone and has multiple functions important in virus replication. The three negative sense RNA segments of hantaviruses form panhandle structures through imperfect hydro...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.7.6.13862
更新日期:2010-11-01 00:00:00
abstract::Fragile X-associated tremor/ataxia syndrome (FXTAS) is a recently identified neurodegenerative disorder affecting older adult males with premutation alleles of the fragile X mental retardation 1 (FMR1) gene. The principal clinical features of FXTAS include progressive intention tremor, gait ataxia, parkinsonism, and a...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.1.2.1035
更新日期:2004-07-01 00:00:00
abstract::With a minimal (250-400 nt), non-protein-coding, circular RNA genome, viroids rely on sequence/structural motifs for replication and colonization of their host plants. These motifs are embedded in a compact secondary structure whose elucidation is crucial to understand how they function. Viroid RNA structure has been ...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2016.1223005
更新日期:2017-08-03 00:00:00
abstract::Changing the abundance of transcripts by regulated RNA degradation is a critical step in the control of various biological pathways. Recently, genome-wide inhibitor-free technologies for determining RNA stabilities in mammalian cells have been developed. In these methods, endogenous RNAs are pulse labeled by uridine a...
journal_title:RNA biology
pub_type: 杂志文章,评审
doi:10.4161/rna.22036
更新日期:2012-10-01 00:00:00
abstract::We had previously proposed that the post-transcriptional regulation through microRNA as a mechanism for incomplete penetrance and variable expressivity, leads to lack of correlation between genotype and phenotype. Here we report the validation of miRNA-target interactions we predicted earlier and demonstrate the regul...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.19318
更新日期:2012-03-01 00:00:00
abstract::Transcription termination delineates the 3' ends of transcripts, prevents otherwise runaway RNA polymerase (RNAP) from intruding into downstream genes and regulatory elements, and enables release of the RNAP for recycling. While other eukaryotic RNAPs require complex cis-signals and/or accessory factors to achieve the...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2015.1116677
更新日期:2016-01-01 00:00:00
abstract::The subcellular localization of the nonsense mediated decay (NMD) of mRNA transcripts bearing premature termination codons has been controversial. Recently, it has been demonstrated that RNA tethering of key mediators of NMD, including human UPF3B, accurately recreates NMD. Here, we have used tethered UPF3B, combined ...
journal_title:RNA biology
pub_type: 杂志文章
doi:
更新日期:2004-05-01 00:00:00
abstract::RNA exosomes are large multi-subunit protein complexes involved in controlled and processive 3' to 5' RNA degradation. Exosomes form large molecular chambers and harbor multiple nuclease sites as well as RNA binding regions. This makes a quantitative kinetic analysis of RNA degradation with reliable parameter and erro...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.8.1.14067
更新日期:2011-01-01 00:00:00
abstract::De novo addition of telomeric sequences can occur at broken chromosomes and must be well controlled, which is essential during programmed DNA reorganization processes. In ciliated protozoa an extreme form of DNA-reorganization is observed during macronuclear differentiation after sexual reproduction leading to the eli...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2015.1134414
更新日期:2016-08-02 00:00:00
abstract::In the last years, a number of nuclear genes restoring cytoplasmic male sterility (CMS) have been cloned in various crop species. The majority of these genes have been shown to encode pentatricopeptide repeat proteins (PPR) that act by specifically suppressing the expression of sterility-causing mitochondrial transcri...
journal_title:RNA biology
pub_type: 杂志文章,评审
doi:10.4161/rna.25568
更新日期:2013-01-01 00:00:00
abstract::Amplification or overexpression of neuronal MYC (MYCN) is associated with poor prognosis of human neuroblastoma. Three isoforms of the MYCN protein have been described as well as a protein encoded by an antisense transcript (MYCNOS) that originates from the opposite strand at the MYCN locus. Recent findings suggest th...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2015.1063773
更新日期:2015-01-01 00:00:00
abstract::Bacterial small RNAs (sRNAs) are a class of structural RNAs that often regulate mRNA targets via post-transcriptional base pair interactions. We determined features that discriminate functional from non-functional interactions and assessed the influence of these features on genome-wide target predictions. For this pur...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.20294
更新日期:2012-07-01 00:00:00
abstract::We report here an in-depth characterization of the aptamer domain of the transcriptional adenine-sensing riboswitch (pbuE) by NMR and fluorescence spectroscopy. By NMR studies, the structure of two aptamer sequences with different lengths of the helix P1, the central element involved in riboswitch conformational switc...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.29439
更新日期:2014-01-01 00:00:00
abstract::The genome of most prokaryotes gives rise to surprisingly complex transcriptomes, comprising not only protein-coding mRNAs, often organized as operons, but also harbors dozens or even hundreds of highly structured small regulatory RNAs and unexpectedly large levels of anti-sense transcripts. Comprehensive surveys of p...
journal_title:RNA biology
pub_type: 杂志文章,评审
doi:10.4161/rna.28647
更新日期:2014-01-01 00:00:00
abstract::In an earlier report two P-transposon insertion alleles of the noncoding hsromega gene, hsromega(05241) and P292 were shown to enhance neurodegeneration caused by expression of ataxin-1 protein with expanded poly-Q in a Drosophila model. In present study, we examined the possible relation between hsromega gene express...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.3.1.2559
更新日期:2006-01-01 00:00:00
abstract::Post-transcriptional control of gene expression is mediated via RNA-binding proteins (RBPs) that interact with mRNAs in a combinatorial fashion. While recent global RNA interactome capture experiments expanded the repertoire of cellular RBPs quiet dramatically, little is known about the assembly of RBPs on particular ...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2019.1662268
更新日期:2020-01-01 00:00:00
abstract::The instructions to make proteins and structural RNAs are laid down in gene sequences. Yet, in certain instances, these primary instructions need to be modified considerably during gene expression, most often at the transcript level. Here we review a case of massive post-transcriptional revisions via trans-splicing an...
journal_title:RNA biology
pub_type: 杂志文章,评审
doi:10.1080/15476286.2016.1240143
更新日期:2016-12-01 00:00:00
abstract::In eukaryotes, the 3' ends of RNA polymerase II-transcribed RNAs are generated in the majority of cases by site-specific endonucleolytic cleavage, followed by the addition of a poly(A) tail. Through alternative polyadenylation, a gene can give rise to multiple mRNA isoforms that differ in the length of their 3' UTRs a...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.22570
更新日期:2012-12-01 00:00:00
abstract::Alternative splicing (AS) in response to changing external conditions often requires alterations in the ability of sequence-specific RNA-binding proteins to bind to cis-acting sequences in their target pre-mRNA. While daily oscillations in AS events have been described in several organisms, cis-acting sequences that c...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2018.1502587
更新日期:2018-01-01 00:00:00
abstract::The Hedgehog (HH) signaling pathway has important roles in tumorigenesis and in embryonal patterning. The Glioma-associated oncogene 1 (GLI1) is a key molecule in HH signaling, acting as a transcriptional effector and, moreover, is considered to be a potential therapeutic target for several types of cancer. To extend ...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.23343
更新日期:2013-02-01 00:00:00
abstract::Viroids are the smallest autonomous infectious nucleic acids known today. They are non-coding, unencapsidated, circular RNAs with sizes ranging from 250 to 400 nucleotides and infect certain plants. These RNAs are transcribed by rolling-circle mechanisms in the plant host's nuclei (Pospiviroidae) or chloroplasts (Avsu...
journal_title:RNA biology
pub_type: 杂志文章,评审
doi:10.4161/rna.19810
更新日期:2012-06-01 00:00:00
abstract::Ribosome biogenesis drives cell growth and proliferation, but mechanisms that modulate this process remain poorly understood. For a long time, small rRNA sequences have been widely treated as non-specific degradation products and neglected as garbage sequences. Recently, we identified a new class of antisense ribosoma...
journal_title:RNA biology
pub_type: 杂志文章,评审
doi:10.1080/15476286.2017.1341034
更新日期:2017-11-02 00:00:00
abstract::The genus Xanthomonas comprises a large group of plant-pathogenic bacteria. The infection and bacterial multiplication in the plant tissue depends on the type III secretion system and other virulence determinants. Recent studies revealed that bacterial virulence is also controlled at the post-transcriptional level by ...
journal_title:RNA biology
pub_type: 杂志文章,评审
doi:10.4161/rna.28240
更新日期:2014-01-01 00:00:00
abstract::Transcriptional regulation of microRNA (miRNA) expression is one of the least understood aspects of miRNA biogenesis. In C. elegans the list of miRNAs whose transcriptional control has been described in some detail is currently limited to four: let-7, lin-4, lsy-6, and mir-61. Each of these genes has been shown experi...
journal_title:RNA biology
pub_type: 杂志文章,评审
doi:10.4161/rna.6.1.7574
更新日期:2009-01-01 00:00:00
abstract::Riboswitches are cis-encoded, cis-acting RNA elements that directly sense a physiological signal. Signal response results in a change in RNA structure that impacts gene expression. Elements of this type play an important role in bacteria, where they regulate a variety of fundamental cellular pathways. Riboswitch-media...
journal_title:RNA biology
pub_type: 杂志文章,评审
doi:10.4161/rna.7.1.10757
更新日期:2010-01-01 00:00:00
abstract::The ribosome binding site of Escherichia coli rpoS mRNA, encoding the stationary sigma-factor RpoS, is sequestered by an inhibitory stem-loop structure (iss). Translational activation of rpoS mRNA at low temperature and during exponential growth includes Hfq-facilitated duplex formation between rpoS and the small regu...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.7.6.13768
更新日期:2010-11-01 00:00:00
abstract::Physical entanglement, and particularly knots arise spontaneously in equilibrated polymers that are sufficiently long and densely packed. Biopolymers are no exceptions: knots have long been known to occur in proteins as well as in encapsidated viral DNA. The rapidly growing number of RNA structures has recently made i...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2015.1132069
更新日期:2016-01-01 00:00:00
abstract::U12-type introns are a rare class of nuclear introns that are removed by a dedicated U12-dependent spliceosome and are thought to regulate the expression of their target genes owing through their slower splicing reaction. Recent genome-wide studies on the splicing of U12-type introns are now providing new insights on ...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2014.996454
更新日期:2014-01-01 00:00:00
abstract::In plants, endogenes are less prone to RNA silencing than transgenes. While both can be efficiently targeted by small RNAs for post-transcriptional gene silencing (PTGS), generally only transgene PTGS is accompanied by transitivity, RNA-directed DNA methylation (RdDM) and systemic silencing. In order to investigate wh...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.29623
更新日期:2014-01-01 00:00:00
abstract::RNA editing in flowering plant mitochondria targets several hundred C nucleotides mostly in mRNAs to be altered to U. Several nuclear encoded genes have been recently identified predominantly in Arabidopsis thaliana which code for proteins involved in specific RNA editing events in plastids or mitochondria. These nucl...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.8.1.14298
更新日期:2011-01-01 00:00:00