Abstract:
:The instructions to make proteins and structural RNAs are laid down in gene sequences. Yet, in certain instances, these primary instructions need to be modified considerably during gene expression, most often at the transcript level. Here we review a case of massive post-transcriptional revisions via trans-splicing and RNA editing, a phenomenon occurring in mitochondria of a recently recognized protist group, the diplonemids. As of now, the various post-transcriptional steps have been cataloged in detail, but how these processes function is still unknown. Since genetic manipulation techniques such as gene replacement and RNA interference have not yet been established for these organisms, alternative strategies have to be deployed. Here, we discuss the experimental and bioinformatics approaches that promise to unravel the molecular machineries of trans-splicing and RNA editing in Diplonema mitochondria.
journal_name
RNA Bioljournal_title
RNA biologyauthors
Valach M,Moreira S,Faktorová D,Lukeš J,Burger Gdoi
10.1080/15476286.2016.1240143subject
Has Abstractpub_date
2016-12-01 00:00:00pages
1204-1211issue
12eissn
1547-6286issn
1555-8584journal_volume
13pub_type
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