Abstract:
:Amplification or overexpression of neuronal MYC (MYCN) is associated with poor prognosis of human neuroblastoma. Three isoforms of the MYCN protein have been described as well as a protein encoded by an antisense transcript (MYCNOS) that originates from the opposite strand at the MYCN locus. Recent findings suggest that some antisense long non-coding RNAs (lncRNAs) can play a role in epigenetically regulating gene expression. Here we report that MYCNOS transcripts function as a modulator of the MYCN locus, affecting MYCN promoter usage and recruiting various proteins, including the Ras GTPase-activating protein-binding protein G3BP1, to the upstream MYCN promoter. Overexpression of MYCNOS results in a reduction of upstream MYCN promoter usage and increased MYCN expression, suggesting that the protein-coding MYCNOS also functions as a regulator of MYCN ultimately controlling MYCN transcriptional variants. The observations presented here demonstrate that protein-coding transcripts can regulate gene transcription and can tether regulatory proteins to target loci.
journal_name
RNA Bioljournal_title
RNA biologyauthors
Vadie N,Saayman S,Lenox A,Ackley A,Clemson M,Burdach J,Hart J,Vogt PK,Morris KVdoi
10.1080/15476286.2015.1063773subject
Has Abstractpub_date
2015-01-01 00:00:00pages
893-9issue
8eissn
1547-6286issn
1555-8584journal_volume
12pub_type
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