RNA-template dependent de novo telomere addition.

Abstract:

:De novo addition of telomeric sequences can occur at broken chromosomes and must be well controlled, which is essential during programmed DNA reorganization processes. In ciliated protozoa an extreme form of DNA-reorganization is observed during macronuclear differentiation after sexual reproduction leading to the elimination of specific parts of the germline genome. Regulating these processes involves small noncoding RNAs, but in addition DNA-reordering, excision and amplification require RNA templates deriving from the parental macronucleus. We show that these putative RNA templates can carry telomeric repeats. Microinjection of RNA templates carrying modified telomeres into the developing macronucleus leads to modified telomeres in vegetative cells, providing strong evidence, that de novo addition of telomeres depends on a telomere-containing transcript from the parental macronucleus.

journal_name

RNA Biol

journal_title

RNA biology

authors

Fuhrmann G,Jönsson F,Weil PP,Postberg J,Lipps HJ

doi

10.1080/15476286.2015.1134414

subject

Has Abstract

pub_date

2016-08-02 00:00:00

pages

733-9

issue

8

eissn

1547-6286

issn

1555-8584

journal_volume

13

pub_type

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