Abstract:
:The subcellular localization of the nonsense mediated decay (NMD) of mRNA transcripts bearing premature termination codons has been controversial. Recently, it has been demonstrated that RNA tethering of key mediators of NMD, including human UPF3B, accurately recreates NMD. Here, we have used tethered UPF3B, combined with regulation of nuclear mRNA export using the retroviral Rev/RRE system, to examine where UPF3B-mediated mRNA degradation occurs. Our data clearly demonstrate that nuclear mRNA export is required for UPF3B-mediated degradation and moreover show that this degradation is exclusively cytoplasmic, despite the fact that the UPF3B fusion protein used is a nucleocytoplasmic shuttle protein localized predominantly to the nucleus. Moreover, exclusively cytoplasmic NMD occurred whether the target mRNA was exported via the retroviral Rev/RRE pathway or via the Tap: Nxt pathway used by most cellular mRNAs. These data may suggest that truly nuclear NMD, if it occurs, is at least in part mechanistically distinct from cytoplasmic NMD.
journal_name
RNA Bioljournal_title
RNA biologyauthors
Lu S,Cullen BRsubject
Has Abstractpub_date
2004-05-01 00:00:00pages
42-7issue
1eissn
1547-6286issn
1555-8584pii
916journal_volume
1pub_type
杂志文章相关文献
RNA Biology文献大全abstract::Transcription termination delineates the 3' ends of transcripts, prevents otherwise runaway RNA polymerase (RNAP) from intruding into downstream genes and regulatory elements, and enables release of the RNAP for recycling. While other eukaryotic RNAPs require complex cis-signals and/or accessory factors to achieve the...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2015.1116677
更新日期:2016-01-01 00:00:00
abstract::The acquisition of antibiotic resistance by human pathogens poses a significant threat to public health. The mechanisms that control the proliferation and expression of antibiotic resistance genes are not yet completely understood. The aminoglycosides are a historically important class of antibiotics that were introdu...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.25757
更新日期:2013-08-01 00:00:00
abstract::YchF is one of two universally conserved GTPases with unknown cellular function. As a first step toward elucidating YchF's cellular role, we performed a detailed biochemical characterization of the protein from Escherichia coli. Our data from fluorescence titrations not only confirmed the surprising finding that YchFE...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.22131
更新日期:2012-10-01 00:00:00
abstract::Population differences observed for complex traits may be attributed to the combined effect of socioeconomic, environmental, genetic and epigenetic factors. To better understand population differences in complex traits, genome-wide genetic and gene expression differences among ethnic populations have been studied. Her...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.8.4.16029
更新日期:2011-07-01 00:00:00
abstract::tRNA molecules undergo extensive post-transcriptional processing to generate the mature functional tRNA species that are essential for translation in all organisms. These processing steps include the introduction of numerous specific chemical modifications to nucleotide bases and sugars; among these modifications, met...
journal_title:RNA biology
pub_type: 杂志文章,评审
doi:10.1080/15476286.2015.1008358
更新日期:2015-01-01 00:00:00
abstract::The question of how cysteinyl-tRNA(Cys) is synthesized in organisms that lack a canonical cysteinyl-tRNA synthetase (CysRS) is an important open question in understanding protein synthesis. The prolyl-tRNA synthetase (ProRS) of wide ranging organisms has the ability to mis-activate cysteine without editing. This raise...
journal_title:RNA biology
pub_type: 杂志文章
doi:
更新日期:2004-05-01 00:00:00
abstract::Nuclear export of mRNA is a critical event in mRNA biogenesis. Passage of mature messenger ribonucleoproteins (mRNPs) through nuclear pore complexes (NPCs) is facilitated by the Mex67/Mtr2 heterodimer. At the NPC cytoplasmic face, the DEAD-box RNA helicase Dbp5 remodels mRNPs by removing Mex67/Mtr2. This remodeling pr...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.7.1.10576
更新日期:2010-01-01 00:00:00
abstract::RNA methylation, catalysed by a set of RNA methyltransferases (RNMTs), modulates RNA structures, properties, and biological functions. RNMTs are increasingly documented to be dysregulated in various human diseases, particularly developmental disorders and cancer. However, the genomic and transcriptomic alterations of ...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2019.1708549
更新日期:2020-04-01 00:00:00
abstract::A very conserved pseudoknot structure has been shown to fold in influenza virus RNA. The pseudoknot encompasses the 3' splice site of segment 8 RNA in both influenza A and B viruses. By sequence comparison of influenza virus strains, we derive a consensus motif that defines a novel RNA pseudoknot family. The orientati...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.7.2.11287
更新日期:2010-03-01 00:00:00
abstract::Conserved RNA secondary structures were predicted in the nucleoprotein (NP) segment of the influenza A virus genome using comparative sequence and structure analysis. A number of structural elements exhibiting nucleotide covariations were identified over the whole segment length, including protein-coding regions. Calc...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.29730
更新日期:2014-01-01 00:00:00
abstract::In a series of conceptual articles published around the millennium, Carl Woese emphasized that evolution of cells is the central problem of evolutionary biology, that the three-domain ribosomal tree of life is an essential framework for reconstructing cellular evolution, and that the evolutionary dynamics of functiona...
journal_title:RNA biology
pub_type: 杂志文章,评审
doi:10.4161/rna.27673
更新日期:2014-01-01 00:00:00
abstract::We report here an in-depth characterization of the aptamer domain of the transcriptional adenine-sensing riboswitch (pbuE) by NMR and fluorescence spectroscopy. By NMR studies, the structure of two aptamer sequences with different lengths of the helix P1, the central element involved in riboswitch conformational switc...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.29439
更新日期:2014-01-01 00:00:00
abstract::RNA-binding proteins (RBPs) are key regulators of posttranscriptional processes such as RNA maturation, localization, turnover and translation. Despite their dysregulation in various diseases including cancer, the landscape of RBP expression in human cancer has not been well elucidated. Here, we built a comprehensive ...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2019.1673657
更新日期:2020-02-01 00:00:00
abstract::The HIV-1 nucleocapsid protein (NC) is a nucleic acid chaperone, which remodels nucleic acid structures so that the most thermodynamically stable conformations are formed. This activity is essential for virus replication and has a critical role in mediating highly specific and efficient reverse transcription. NC's fun...
journal_title:RNA biology
pub_type: 杂志文章,评审
doi:10.4161/rna.7.6.14115
更新日期:2010-11-01 00:00:00
abstract::Fragile X-associated tremor/ataxia syndrome (FXTAS) is a recently identified neurodegenerative disorder affecting older adult males with premutation alleles of the fragile X mental retardation 1 (FMR1) gene. The principal clinical features of FXTAS include progressive intention tremor, gait ataxia, parkinsonism, and a...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.1.2.1035
更新日期:2004-07-01 00:00:00
abstract::Ribonuclease P (RNase P) is a ribonucleoprotein comprised of a catalytic RNA subunit and one or several protein subunits. RNase P is best known for its role in 5'-processing of tRNA precursors. RNase P enzymes from almost all forms of life, including protein-synthesizing organelles, contain an RNase P with a conserved...
journal_title:RNA biology
pub_type: 杂志文章,评审
doi:10.4161/rna.6.4.9241
更新日期:2009-09-01 00:00:00
abstract::Human Enterovirus 71 (EV71) is an emerging pathogen of infectious disease and a serious threat to public health. Currently, there are no antivirals or vaccines to slow down or prevent EV71 infections, thus underscoring the urgency to better understand mechanisms of host-enterovirus interactions. EV71 uses a type I int...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.25107
更新日期:2013-07-01 00:00:00
abstract::Seryl-tRNA synthetase (SerRS) catalyzes the ligation of serine to the 3'-end of serine tRNA (tRNA(Ser)), which is typical of the type-2 tRNAs characterized by a long extra arm. The SerRSs are divided into two types, the archaeal/eukaryal and bacterial types. In this study, we solved the crystal structures of the SerRS...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.5.3.6876
更新日期:2008-07-01 00:00:00
abstract::The RNase II family of enzymes is ubiquitous and has crucial roles in the processing, degradation and quality control of all types of RNA. These exoribonucleases processively degrade RNA from the 3'-end releasing 5'-nucleotide monophosphates. In prokaryotes, RNase II and RNase R have two N-terminal CSD and one C-termi...
journal_title:RNA biology
pub_type: 杂志文章,评审
doi:10.4161/rna.7.3.11490
更新日期:2010-05-01 00:00:00
abstract::RNA editing in flowering plant mitochondria targets several hundred C nucleotides mostly in mRNAs to be altered to U. Several nuclear encoded genes have been recently identified predominantly in Arabidopsis thaliana which code for proteins involved in specific RNA editing events in plastids or mitochondria. These nucl...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.8.1.14298
更新日期:2011-01-01 00:00:00
abstract::The development of rigorous molecular taxonomy pioneered by Carl Woese has freed evolution science to explore numerous cellular activities that lead to genome change in evolution. These activities include symbiogenesis, inter- and intracellular horizontal DNA transfer, incorporation of DNA from infectious agents, and ...
journal_title:RNA biology
pub_type: 杂志文章,评审
doi:10.4161/rna.27506
更新日期:2014-01-01 00:00:00
abstract::Amplification or overexpression of neuronal MYC (MYCN) is associated with poor prognosis of human neuroblastoma. Three isoforms of the MYCN protein have been described as well as a protein encoded by an antisense transcript (MYCNOS) that originates from the opposite strand at the MYCN locus. Recent findings suggest th...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2015.1063773
更新日期:2015-01-01 00:00:00
abstract::Oncogenic viruses are associated with approximately 15% of human cancers. In viral infections, microRNAs play an important role in host-pathogen interactions. miR-21 is a highly conserved non-coding RNA that not only regulates the development of oncogenic viral diseases, but also responds to the regulation of intracel...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2021.1880756
更新日期:2021-01-27 00:00:00
abstract::The immaculate annotation of all microRNAs (miRNAs) is a prerequisite to study their biological function on a genome-wide scale. However, the original criteria for proper miRNA annotation seem unsuited for the automated analysis of the immense number of small RNA reads available in next generation sequencing (NGS) dat...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.8.3.14333
更新日期:2011-05-01 00:00:00
abstract::Nonsense-mediated decay (NMD) is a RNA surveillance pathway that degrades subsets of normal and aberrant mRNAs. Mutations that perturb NMD cause neurological disorders in humans, suggesting that NMD has roles in the brain. Recently, it was shown that NMD is repressed during neural development to allow for the stabiliz...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.9.1.18010
更新日期:2012-01-01 00:00:00
abstract::Viroids are non-coding single-stranded circular RNA molecules that replicate autonomously in infected host plants causing mild to lethal symptoms. Their genomes contain about 250-400 nucleotides, depending on viroid species. Members of the family Pospiviroidae, like the Potato spindle tuber viroid (PSTVd), replicate v...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2016.1272745
更新日期:2017-03-04 00:00:00
abstract::The duck represents an important reservoir of influenza viruses for transmission to other avian and mammalian hosts, including humans. The increased pathogenicity of the recently emerging clades of highly pathogenic avian influenza (HPAI) viruses of the H5N1 subtype in ducks features systemic viral spread and organ-to...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2019.1669879
更新日期:2020-01-01 00:00:00
abstract::Alzheimer's disease (AD) has devastating consequences for patients during its slow, progressive course. It is important to understand the pathology of AD onset. Recently, circular RNAs (circRNAs) have been found to participate in many human diseases including cancers and neurodegenerative conditions. In this study, we...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2020.1783487
更新日期:2020-12-01 00:00:00
abstract::Circular RNAs (circRNAs) constitute a large class of RNA species formed by the back-splicing of co-linear exons, often within protein-coding transcripts. Despite much progress in the field, it remains elusive whether the majority of circRNAs are merely aberrant splicing by-products with unknown functions, or their pro...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2016.1207036
更新日期:2016-09-01 00:00:00
abstract::tmRNA employs both tRNA-like and mRNA-like properties as it rescues stalled bacterial ribosomes, while targeting the defective mRNA and incomplete nascent protein for degradation. We describe variation of the tmRNA gene (ssrA) and how it informs tmRNA structure and function. Endosymbiont tmRNAs tend to lose secondary ...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.6.4.9172
更新日期:2009-09-01 00:00:00